Structured Review

Abcam mouse anti occludin
TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker <t>occludin.</t> Representative images
Mouse Anti Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti occludin/product/Abcam
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti occludin - by Bioz Stars, 2020-08
91/100 stars

Images

1) Product Images from "Endothelial Surface N-Glycans Mediate Monocyte Adhesion and Are Targets for Anti-inflammatory Effects of Peroxisome Proliferator-activated Receptor ? Ligands *"

Article Title: Endothelial Surface N-Glycans Mediate Monocyte Adhesion and Are Targets for Anti-inflammatory Effects of Peroxisome Proliferator-activated Receptor ? Ligands *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M111.247981

TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker occludin. Representative images
Figure Legend Snippet: TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker occludin. Representative images

Techniques Used: Staining, Marker

Related Articles

Incubation:

Article Title: Endothelial Surface N-Glycans Mediate Monocyte Adhesion and Are Targets for Anti-inflammatory Effects of Peroxisome Proliferator-activated Receptor ? Ligands *
Article Snippet: .. After blocking, coverslips were incubated with mouse anti-occludin (1:200, Abcam) in PBS containing 5% goat serum overnight at 4 °C. .. Following washing, cells were incubated with goat anti-mouse Alexa Fluor 594 (1:1000, Molecular Probes) in PBS with 5% goat serum for 1 h at RT.

Immunofluorescence:

Article Title: Novel mechanism regulating endothelial permeability via T-cadherin-dependent VE-cadherin phosphorylation and clathrin-mediated endocytosis
Article Snippet: .. Antibodies and reagents The following antibodies were used: rabbit anti-T-cad (ProSci Inc.); mouse anti-VE-cadherin, rabbit anti-phospho VE-cadherin (Y658), and rabbit anti-phospho VE-cadherin (Y731) (Abcam); mouse anti-N-cadherin (Santa Cruz); mouse anti-β-catenin and mouse anti-phospho-β-catenin (T41) (Abcam); mouse anti-p120ctn (Santa Cruz); rabbit anti-clathrin (Abcam); rabbit anti- PAK1 and rabbit anti-phospho-PAK1 (T212) (Abcam); rabbit anti-ROCK-II (Santa Cruz); rabbit anti-Src and rabbit anti-phospho-Src (Y418) (Abcam); mouse anti-p38 (Sigma) and rabbit anti-phospho-p38 (T180+Y182) (Abcam); rabbit anti-Erk1+Erk2 ant rabbit anti-phospho-Erk1+Erk2 (T185+T202) (Abcam); mouse anti-ZO-1 (Invitrogen); mouse anti-occludin (Abcam); mouse anti-claudin-5 (Invitrogen); mouse anti caveolin-1 (BD); rabbit anti-EEA1 (Abcam); rabbit anti-LAMP1 (Abcam); LysotrackerRed® (Invitrogen); phalloidin AlexaFluor® 488 (Molecular Probes); secondary anti-mouse and anti-rabbit AlexaFluor® 488 or AlexaFluor® 594 antibodies (Molecular Probes) were used in immunofluorescence techniques. .. Rabbit anti-GAPDH (Santa Cruz) antibody was used in Western blotting for protein loading control; the secondary antibodies in Western blotting were HRP-conjugated donkey anti-mouse or donkey anti-rabbit IgG (Jackson Immuno-Research Laboratories).

Blocking Assay:

Article Title: Endothelial Surface N-Glycans Mediate Monocyte Adhesion and Are Targets for Anti-inflammatory Effects of Peroxisome Proliferator-activated Receptor ? Ligands *
Article Snippet: .. After blocking, coverslips were incubated with mouse anti-occludin (1:200, Abcam) in PBS containing 5% goat serum overnight at 4 °C. .. Following washing, cells were incubated with goat anti-mouse Alexa Fluor 594 (1:1000, Molecular Probes) in PBS with 5% goat serum for 1 h at RT.

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  • 91
    Abcam mouse anti occludin
    TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker <t>occludin.</t> Representative images
    Mouse Anti Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti occludin/product/Abcam
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti occludin - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    99
    Abcam anti occludin
    Representative micrographs of distribution of <t>occludin</t> between groups. The tight junctions protein Occludin (green) and nuclei (blue) images were obtained by immunofluorescence analysis of tissue sections of rats jejunums (200X). Arrows indicate areas of staining. Sections from at least 4 mice were examined for each condition.
    Anti Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti occludin/product/Abcam
    Average 99 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    anti occludin - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Abcam rabbit anti occludin
    USP53 interacts with TJP1 and TJP2 via its C-terminal tail. A , GFP–USP53 fusion constructs, depicted schematically in C , were injectoporated into cochlear explants from P4 wild-type (WT) mice. GFP fusion proteins containing the C-terminal tail of USP53 localized to TJs. B , GFP and GFP–USP53 fusion constructs were expressed in HEK293T cells and immunoprecipitated with anti-GFP antibody (Abcam). Extracts were directly analyzed for the expression of GFP-tagged USP53 constructs using anti-GFP antibody (Sigma). An asterisk marks a nonspecific cross-reacting band at ∼80 kDa used as a loading control. Native TJ proteins TJP1 and TJP2, but not <t>occludin</t> (OCLN), coprecipitated specifically with GFP fusion proteins that contain the C-terminal tail of USP53. IB, Immunoblot; IP, immunoprecipitation. C , Schematic diagram of the GFP–USP53 fusion proteins and TJPs used for biochemical experiments and their predicted molecular sizes. D , HEK293T cells were transfected with the constructs indicated on top of each panel. Immunoprecipitations were performed with anti-GFP antibody (Abcam) that recognizes GFP–USP53 fusion proteins followed by Western blotting with anti-FLAG antibody or anti-GFP antibody (Abcam). 2xFLAG–TJP2 coprecipitated with TJP2–GFP (TJP2 homodimerization), as well as with GFP–USP53 constructs containing a stretch of 328 aa adjacent to the catalytic domain. Neither the catalytic domain nor GFP alone precipitated 2xFLAG–TJP2. E , Schematic representation of C-terminal deletion constructs of USP53 used in D . The region containing the TJP2 interaction site is highlighted in blue. Scale bar: A , 5 μm.
    Rabbit Anti Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti occludin/product/Abcam
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    rabbit anti occludin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker occludin. Representative images

    Journal: The Journal of Biological Chemistry

    Article Title: Endothelial Surface N-Glycans Mediate Monocyte Adhesion and Are Targets for Anti-inflammatory Effects of Peroxisome Proliferator-activated Receptor ? Ligands *

    doi: 10.1074/jbc.M111.247981

    Figure Lengend Snippet: TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker occludin. Representative images

    Article Snippet: After blocking, coverslips were incubated with mouse anti-occludin (1:200, Abcam) in PBS containing 5% goat serum overnight at 4 °C.

    Techniques: Staining, Marker

    Representative micrographs of distribution of occludin between groups. The tight junctions protein Occludin (green) and nuclei (blue) images were obtained by immunofluorescence analysis of tissue sections of rats jejunums (200X). Arrows indicate areas of staining. Sections from at least 4 mice were examined for each condition.

    Journal: PLoS ONE

    Article Title: Comparison of Melatonin, Hypertonic Saline, and Hydroxyethyl Starch for Resuscitation of Secondary Intra-Abdominal Hypertension in an Animal Model

    doi: 10.1371/journal.pone.0161688

    Figure Lengend Snippet: Representative micrographs of distribution of occludin between groups. The tight junctions protein Occludin (green) and nuclei (blue) images were obtained by immunofluorescence analysis of tissue sections of rats jejunums (200X). Arrows indicate areas of staining. Sections from at least 4 mice were examined for each condition.

    Article Snippet: The membranes were incubated with anti-phospho-Akt (1:2000; Cell Signaling Technology, Danvers, MA, USA), anti-Akt (1:1000; Cell Signaling Technology, Danvers, MA, USA), anti- zonula occludens-1(ZO-1) (1:50; Abcam, Cambridge, MA, USA), and anti-occludin (1:100; Abcam, Cambridge, MA, USA) antibodies overnight at 4°C.

    Techniques: Immunofluorescence, Staining, Mouse Assay

    Expression of total and phosphorylated protein kinase B (p-Akt) and tight junction proteins in intestine between groups. The intestinal sample extracts were immunoblotted for the expression of phosphorylated (p)-Akt, total (t)-Akt, ZO-1 and occludin. Representative images of the Western blots are shown (A). Expression of p-Akt and t-Akt among groups (B); Expression of ZO-1 among groups; (C). Expression of occludin among groups. Data are presented as means ± S.E.M., n = 8 in each group. Data are presented as means ± S.E.M., n = 8 in each group. * P

    Journal: PLoS ONE

    Article Title: Comparison of Melatonin, Hypertonic Saline, and Hydroxyethyl Starch for Resuscitation of Secondary Intra-Abdominal Hypertension in an Animal Model

    doi: 10.1371/journal.pone.0161688

    Figure Lengend Snippet: Expression of total and phosphorylated protein kinase B (p-Akt) and tight junction proteins in intestine between groups. The intestinal sample extracts were immunoblotted for the expression of phosphorylated (p)-Akt, total (t)-Akt, ZO-1 and occludin. Representative images of the Western blots are shown (A). Expression of p-Akt and t-Akt among groups (B); Expression of ZO-1 among groups; (C). Expression of occludin among groups. Data are presented as means ± S.E.M., n = 8 in each group. Data are presented as means ± S.E.M., n = 8 in each group. * P

    Article Snippet: The membranes were incubated with anti-phospho-Akt (1:2000; Cell Signaling Technology, Danvers, MA, USA), anti-Akt (1:1000; Cell Signaling Technology, Danvers, MA, USA), anti- zonula occludens-1(ZO-1) (1:50; Abcam, Cambridge, MA, USA), and anti-occludin (1:100; Abcam, Cambridge, MA, USA) antibodies overnight at 4°C.

    Techniques: Expressing, Western Blot

    EPS-1 Relieved DSS-Induced Colonic Epithelial Tight Junction Disruption in Mice. ( A ) Relative protein levels of Occludin, E-cadherin and Claudin1 in the colon tissues normalized to β-actin. ( B ) Protein expression of Occludin, E-cadherin, and Claudin-1 in the colon tissues were analyzed by Western blot. Values with different superscript letters (a, b,) are significantly different ( p

    Journal: Molecules

    Article Title: A Role of Exopolysaccharide Produced by Streptococcus thermophilus in the Intestinal Inflammation and Mucosal Barrier in Caco-2 Monolayer and Dextran Sulphate Sodium-Induced Experimental Murine Colitis

    doi: 10.3390/molecules24030513

    Figure Lengend Snippet: EPS-1 Relieved DSS-Induced Colonic Epithelial Tight Junction Disruption in Mice. ( A ) Relative protein levels of Occludin, E-cadherin and Claudin1 in the colon tissues normalized to β-actin. ( B ) Protein expression of Occludin, E-cadherin, and Claudin-1 in the colon tissues were analyzed by Western blot. Values with different superscript letters (a, b,) are significantly different ( p

    Article Snippet: Then the proteins were electro-transferred to PVDF member at 200 mA for 2 h. The membranes were incubated with primary antibodies: anti-β-actin (1:2500, Bioss, Beijing, China), Anti-Occludin (1:50,000, Abcam, Cambridge, MA, USA), anti-E-cadherin (1:1000, Abcam, USA), anti-Claudin1 (1:2000, Abcam, USA) for 12 h at 4 °C.

    Techniques: Mouse Assay, Expressing, Western Blot

    EPS-1 Protected intestinal barrier integrity from the disruption by LPS in Caco-2 monolayer. ( A ) The changes of transepithelial electrical resistance (TER) before and after treatment with LPS (10 μg/mL) and/or EPS-1 (2 mg/mL) for 24 h in Caco-2 monolayer. ( B ) FITC-dextran (4 kDa) permeability measurement in 21-day cultured Caco-2 monolayer after LPS and/or EPS-1 treatment. ( C ) The levels of pro-inflammatory cytokines in the Caco-2 cells from each treatment group. ( D ) Protein expression of Occludin, E-cadherin and Claudin1 in the Caco-2 cells from each treatment group. ( E ) Relative protein levels of Occludin, E-cadherin and Claudin1 in Caco-2 cells normalized to β-actin. Values with different superscript letters (a, b, c) are significantly different ( p

    Journal: Molecules

    Article Title: A Role of Exopolysaccharide Produced by Streptococcus thermophilus in the Intestinal Inflammation and Mucosal Barrier in Caco-2 Monolayer and Dextran Sulphate Sodium-Induced Experimental Murine Colitis

    doi: 10.3390/molecules24030513

    Figure Lengend Snippet: EPS-1 Protected intestinal barrier integrity from the disruption by LPS in Caco-2 monolayer. ( A ) The changes of transepithelial electrical resistance (TER) before and after treatment with LPS (10 μg/mL) and/or EPS-1 (2 mg/mL) for 24 h in Caco-2 monolayer. ( B ) FITC-dextran (4 kDa) permeability measurement in 21-day cultured Caco-2 monolayer after LPS and/or EPS-1 treatment. ( C ) The levels of pro-inflammatory cytokines in the Caco-2 cells from each treatment group. ( D ) Protein expression of Occludin, E-cadherin and Claudin1 in the Caco-2 cells from each treatment group. ( E ) Relative protein levels of Occludin, E-cadherin and Claudin1 in Caco-2 cells normalized to β-actin. Values with different superscript letters (a, b, c) are significantly different ( p

    Article Snippet: Then the proteins were electro-transferred to PVDF member at 200 mA for 2 h. The membranes were incubated with primary antibodies: anti-β-actin (1:2500, Bioss, Beijing, China), Anti-Occludin (1:50,000, Abcam, Cambridge, MA, USA), anti-E-cadherin (1:1000, Abcam, USA), anti-Claudin1 (1:2000, Abcam, USA) for 12 h at 4 °C.

    Techniques: Permeability, Cell Culture, Expressing

    USP53 interacts with TJP1 and TJP2 via its C-terminal tail. A , GFP–USP53 fusion constructs, depicted schematically in C , were injectoporated into cochlear explants from P4 wild-type (WT) mice. GFP fusion proteins containing the C-terminal tail of USP53 localized to TJs. B , GFP and GFP–USP53 fusion constructs were expressed in HEK293T cells and immunoprecipitated with anti-GFP antibody (Abcam). Extracts were directly analyzed for the expression of GFP-tagged USP53 constructs using anti-GFP antibody (Sigma). An asterisk marks a nonspecific cross-reacting band at ∼80 kDa used as a loading control. Native TJ proteins TJP1 and TJP2, but not occludin (OCLN), coprecipitated specifically with GFP fusion proteins that contain the C-terminal tail of USP53. IB, Immunoblot; IP, immunoprecipitation. C , Schematic diagram of the GFP–USP53 fusion proteins and TJPs used for biochemical experiments and their predicted molecular sizes. D , HEK293T cells were transfected with the constructs indicated on top of each panel. Immunoprecipitations were performed with anti-GFP antibody (Abcam) that recognizes GFP–USP53 fusion proteins followed by Western blotting with anti-FLAG antibody or anti-GFP antibody (Abcam). 2xFLAG–TJP2 coprecipitated with TJP2–GFP (TJP2 homodimerization), as well as with GFP–USP53 constructs containing a stretch of 328 aa adjacent to the catalytic domain. Neither the catalytic domain nor GFP alone precipitated 2xFLAG–TJP2. E , Schematic representation of C-terminal deletion constructs of USP53 used in D . The region containing the TJP2 interaction site is highlighted in blue. Scale bar: A , 5 μm.

    Journal: The Journal of Neuroscience

    Article Title: Progressive Hearing Loss in Mice Carrying a Mutation in Usp53

    doi: 10.1523/JNEUROSCI.1965-15.2015

    Figure Lengend Snippet: USP53 interacts with TJP1 and TJP2 via its C-terminal tail. A , GFP–USP53 fusion constructs, depicted schematically in C , were injectoporated into cochlear explants from P4 wild-type (WT) mice. GFP fusion proteins containing the C-terminal tail of USP53 localized to TJs. B , GFP and GFP–USP53 fusion constructs were expressed in HEK293T cells and immunoprecipitated with anti-GFP antibody (Abcam). Extracts were directly analyzed for the expression of GFP-tagged USP53 constructs using anti-GFP antibody (Sigma). An asterisk marks a nonspecific cross-reacting band at ∼80 kDa used as a loading control. Native TJ proteins TJP1 and TJP2, but not occludin (OCLN), coprecipitated specifically with GFP fusion proteins that contain the C-terminal tail of USP53. IB, Immunoblot; IP, immunoprecipitation. C , Schematic diagram of the GFP–USP53 fusion proteins and TJPs used for biochemical experiments and their predicted molecular sizes. D , HEK293T cells were transfected with the constructs indicated on top of each panel. Immunoprecipitations were performed with anti-GFP antibody (Abcam) that recognizes GFP–USP53 fusion proteins followed by Western blotting with anti-FLAG antibody or anti-GFP antibody (Abcam). 2xFLAG–TJP2 coprecipitated with TJP2–GFP (TJP2 homodimerization), as well as with GFP–USP53 constructs containing a stretch of 328 aa adjacent to the catalytic domain. Neither the catalytic domain nor GFP alone precipitated 2xFLAG–TJP2. E , Schematic representation of C-terminal deletion constructs of USP53 used in D . The region containing the TJP2 interaction site is highlighted in blue. Scale bar: A , 5 μm.

    Article Snippet: Additional antibodies were as follows: mouse anti-Sox2 (Santa Cruz Biotechnology), rabbit anti-myosin VIIa (Proteus Biosciences), mouse anti-calbindin (Sigma), chicken anti-GFP (Rockland), rabbit anti-GFP (Sigma), rabbit anti-GFP (Abcam), rabbit anti-ZO-1 and anti-ZO-2 (Life Technologies), rabbit anti-occludin (Abcam), rabbit anti-UCHL3 (Cell Signaling Technology), anti-ZO-1 Alexa Fluor 488 conjugate (Life Technologies), anti-FLAG horseradish peroxidase (HRP) conjugate (Sigma), Alexa Fluor 488 and 568 anti-rabbit and anti-mouse (Life Technologies), Alexa Fluor 488 anti-chicken (Life Technologies), Alexa Fluor 350 and 488 phalloidin (Life Technologies), and HRP-conjugated anti-rabbit (GE Healthcare).

    Techniques: Construct, Mouse Assay, Immunoprecipitation, Expressing, Transfection, Western Blot