mouse anti mers cov n iggs  (Sino Biological)


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    Name:
    MERS CoV Spike Protein S1 Antibody Mouse MAb
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant MERS CoV NCoV Novel coronavirus Spike Protein S1 Catalog 40069 V08H AFS88936 1 Met1 Glu725 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    40069-MM23
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    MERS CoV
    Applications:
    ELISA
    Immunogen:
    Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein S1 Protein (Catalog#40069-V08H)
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-RBD Antibody, Anti-S Antibody, Anti-s1 Antibody, Anti-Spike RBD Antibody
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
    Buy from Supplier


    Structured Review

    Sino Biological mouse anti mers cov n iggs
    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold <t>MERS-CoV</t> suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant MERS CoV NCoV Novel coronavirus Spike Protein S1 Catalog 40069 V08H AFS88936 1 Met1 Glu725 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/mouse anti mers cov n iggs/product/Sino Biological
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti mers cov n iggs - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV"

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV

    Journal: Journal of Virology

    doi: 10.1128/JVI.01622-20

    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P
    Figure Legend Snippet: Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P

    Techniques Used: Incubation, Confocal Microscopy, MANN-WHITNEY

    HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P
    Figure Legend Snippet: HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P

    Techniques Used: Incubation, Confocal Microscopy

    2) Product Images from "HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV"

    Article Title: HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV

    Journal: bioRxiv

    doi: 10.1101/2020.03.29.014183

    HTCC blocks interaction between the virus and its entry receptor. Pre-cooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or mock in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red) and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Co-localisation of DPP4 with MERS-CoV-N was determined by confocal microscopy and is presented as Manders’ M2 coefficient. The decrease in colocalization was statistically significant (P
    Figure Legend Snippet: HTCC blocks interaction between the virus and its entry receptor. Pre-cooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or mock in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red) and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Co-localisation of DPP4 with MERS-CoV-N was determined by confocal microscopy and is presented as Manders’ M2 coefficient. The decrease in colocalization was statistically significant (P

    Techniques Used: Incubation, Confocal Microscopy

    Coronavirus internalization into susceptible cells is hampered by HTCC. (A) Pre-cooled HAE cultures were incubated with ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in PFA and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of three independent experiments, each performed in triplicate. * P
    Figure Legend Snippet: Coronavirus internalization into susceptible cells is hampered by HTCC. (A) Pre-cooled HAE cultures were incubated with ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in PFA and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of three independent experiments, each performed in triplicate. * P

    Techniques Used: Incubation, Confocal Microscopy

    3) Product Images from "HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV"

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV

    Journal: Journal of Virology

    doi: 10.1128/JVI.01622-20

    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P
    Figure Legend Snippet: Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P

    Techniques Used: Incubation, Confocal Microscopy, MANN-WHITNEY

    HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P
    Figure Legend Snippet: HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P

    Techniques Used: Incubation, Confocal Microscopy

    Related Articles

    Incubation:

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV
    Article Snippet: Immunostaining and confocal imaging.Fixed cells were permeabilized with 0.1% Triton X-100–1× PBS and incubated overnight at 4°C in 1× PBS supplemented with 5% bovine serum albumin (BSA) and 0.5% Tween 20. .. To visualize MERS-CoV particles, cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (Sino Biological, China) (1:1,000 dilution), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (Thermo Fisher Scientific, Poland) (2.5 μg/ml). .. Actin filaments were stained using phalloidin coupled with Alexa Fluor 633 (Thermo Fisher Scientific, Poland) (0.2 U/ml).

    Article Title: HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV
    Article Snippet: Immunostaining and confocal imagingFixed cells were permeabilized with 0.1% Triton X-100 in 1 × PBS and incubated overnight at 4°C in 1× PBS supplemented with 5% bovine serum albumin (BSA) and 0.5% Tween 20. .. To visualize MERS-CoV particles, cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml; Thermo Fisher Scientific, Poland). .. Actin filaments was stained using phalloidin coupled with Alexa Fluor 633 (0.2 U/ml; Thermo Fisher Scientific, Poland).

    other:

    Article Title: Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells
    Article Snippet: The MERS-CoV spike was detected with either the in-house mouse anti-MERS-CoV spike immune serum or a rabbit anti-MERS-CoV spike antibody from Sino Biological (40069-RP02).

    Blocking Assay:

    Article Title: Antagonism of dsRNA-Induced Innate Immune Pathways by NS4a and NS4b Accessory Proteins during MERS Coronavirus Infection
    Article Snippet: Blots were probed sequentially with antibodies and between antibody treatments were stripped using Thermo Scientific Restore Western blot stripping buffer (catalog no. 21059). .. Blots were blocked with 5% nonfat milk and probed with the following antibodies diluted in the same blocking buffer: anti-PKR (phospho-T446 [E120]) rabbit monoclonal antibody (MAb) at 1:1,000 (Abcam 32036), anti-PKR (D7F7) rabbit MAb at 1:1,000 (Cell Signaling Technology no. 12297), anti-GAPDH (anti-glyceraldehyde-3-phosphate dehydrogenase [14C10]) rabbit MAb (Cell Signaling Technology no. 2118) at 1:1,000, SinoBiological anti-MERS N mouse MAb at 1:1,000, anti-NS4a rabbit serum at 1:500 (obtained from Luis Enjuanes, Spanish National Centre for Biotechnology) , and anti-NS4b rabbit serum at 1:500 (obtained from Robert Silverman, Cleveland Clinic) ( ). .. Lysates were harvested and run on SDS-PAGE gels as described above.

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  • 99
    Sino Biological mouse anti mers cov n iggs
    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold <t>MERS-CoV</t> suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P
    Mouse Anti Mers Cov N Iggs, supplied by Sino Biological, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti mers cov n iggs/product/Sino Biological
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti mers cov n iggs - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    95
    Sino Biological mers cov nucleocapsid protein rabbit antibody
    Single-dose vaccination with ChAdOx1 <t>MERS</t> protects rhesus macaques against bronchointerstitial pneumonia caused by <t>MERS-CoV</t> challenge. Rhesus macaques were vaccinated with a prime-boost or prime-only regimen of ChAdOx1 MERS, or with ChAdOx1 GFP and challenged with MERS-CoV. (A) Ventrodorsal thoracic radiographs collected on 6 DPI. A marker (R) indicates right side of animal. No pathologic changes were observed in animals vaccinated with ChAdOx1 MERS via a prime-boost or prime-only regimen. Animal vaccinated with ChAdOx1 GFP shows focally extensive area of increased pulmonary opacity and deviation of the cardiac silhouette, highlighted in the circle located in the middle and caudal lung lobes. (B) Thoracic radiographs of each animal were scored per lung lobe resulting in a maximum score of 18. Values were averaged per group per day, shown is mean with standard deviation. See Table S2 for more details. (C) Gross pathology of lungs shows no pathologic changes in ChAdOx1 MERS vaccinated animals, and focally extensive areas of consolidation in left cranial, middle and caudal lung lobes in control animals (asterisks). (D) Gross lung lesions were scored for each lung lobe, ventral and dorsal. Values were averaged per group, shown is mean with standard deviation. (E) Lung tissue sections were stained with hematoxylin and eosin. Moderate numbers of lymphocyte accumulation around pulmonary arterioles (asterisks), and mild thickening of alveolar septae by lymphocytes and macrophages (arrow) in lung tissue of animals vaccinated with ChAdOx1 MERS. Marked bronchointerstitial pneumonia with abundant pulmonary edema and fibrin (asterisks), type II pneumocyte hyperplasia (arrow), and increased numbers of alveolar macrophages (arrowhead) in lung tissue of control animals. Magnification = 200x. (F) Lung to body weight ratio was determined for all animals at necropsy. Shown is mean with standard deviation. (G) Lung tissue sections were stained with antibody against MERS-CoV antigen, which is visible as a red-brown staining. No immunoreactivity was found in ChAdOx1 MERS vaccinated animals, whereas multifocal immunoreactivity of type I and II pneumocytes could be found in lung tissue of ChAdOx1 GFP vaccinated animals. (H) Lung tissue sections were scored on severity of lesions (0=no lesions, 1=1-10%, 2=11-25%, 3=26-50%, 4=51-75%, 5=76-100%) and averaged per group. Shown is mean with standard deviation. A = bronchointerstitial pneumonia; B = type II pneumocyte hyperplasia; C = Hemorrhages, edema, fibrin deposits. Statistical significance between groups was determined via two-tailed unpaired Student’s t-test. * = p-value
    Mers Cov Nucleocapsid Protein Rabbit Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov nucleocapsid protein rabbit antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov nucleocapsid protein rabbit antibody - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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    Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P

    Journal: Journal of Virology

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV

    doi: 10.1128/JVI.01622-20

    Figure Lengend Snippet: Coronavirus internalization into susceptible cells is hampered by HTCC. (A and B) Precooled HAE cultures were incubated with an ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in paraformaldehyde (PFA) and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of results from three independent experiments, each performed in triplicate. Mann-Whitney test, *** * , P

    Article Snippet: Mouse anti-MERS-CoV N IgGs (Sino Biological, China) (1:1,000 dilution) and horseradish peroxidase (HRP)-labeled rabbit anti-mouse IgG (Dako, Denmark) (65 ng/ml) were used to detect MERS-CoV nucleocapsid protein.

    Techniques: Incubation, Confocal Microscopy, MANN-WHITNEY

    HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P

    Journal: Journal of Virology

    Article Title: HTCC as a Polymeric Inhibitor of SARS-CoV-2 and MERS-CoV

    doi: 10.1128/JVI.01622-20

    Figure Lengend Snippet: HTCC blocks the interaction between the virus and its entry receptor. Precooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or subjected to mock treatment in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red), and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Colocalization of DPP4 with MERS-CoV-N was determined by confocal microscopy, and the results are presented as Manders’ M2 coefficient values after excluding nonspecific nuclear signal. Colocalization analysis was carried out with ImageJ JACoP (Just Another Colocalization Plugin). The decrease in colocalization was statistically significant ( P

    Article Snippet: Mouse anti-MERS-CoV N IgGs (Sino Biological, China) (1:1,000 dilution) and horseradish peroxidase (HRP)-labeled rabbit anti-mouse IgG (Dako, Denmark) (65 ng/ml) were used to detect MERS-CoV nucleocapsid protein.

    Techniques: Incubation, Confocal Microscopy

    HTCC blocks interaction between the virus and its entry receptor. Pre-cooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or mock in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red) and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Co-localisation of DPP4 with MERS-CoV-N was determined by confocal microscopy and is presented as Manders’ M2 coefficient. The decrease in colocalization was statistically significant (P

    Journal: bioRxiv

    Article Title: HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV

    doi: 10.1101/2020.03.29.014183

    Figure Lengend Snippet: HTCC blocks interaction between the virus and its entry receptor. Pre-cooled Huh7 cells were incubated for 3 h at 4°C with ice-cold MERS-CoV or mock in the presence or absence of HTCC-63 (100 μg/ml). Next, cells were fixed with PFA and immunostained for MERS-CoV-N (green), DPP4 (red) and nuclear DNA (blue). MERS-CoV interaction with the DPP4 protein was analyzed with confocal microscopy. Co-localisation of DPP4 with MERS-CoV-N was determined by confocal microscopy and is presented as Manders’ M2 coefficient. The decrease in colocalization was statistically significant (P

    Article Snippet: To visualize MERS-CoV particles, cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml; Thermo Fisher Scientific, Poland).

    Techniques: Incubation, Confocal Microscopy

    Coronavirus internalization into susceptible cells is hampered by HTCC. (A) Pre-cooled HAE cultures were incubated with ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in PFA and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of three independent experiments, each performed in triplicate. * P

    Journal: bioRxiv

    Article Title: HTCC as a highly effective polymeric inhibitor of SARS-CoV-2 and MERS-CoV

    doi: 10.1101/2020.03.29.014183

    Figure Lengend Snippet: Coronavirus internalization into susceptible cells is hampered by HTCC. (A) Pre-cooled HAE cultures were incubated with ice-cold MERS-CoV suspension in the presence or absence of HTCC-63 (200 μg/ml) for 2 h at 37°C. Next, cells were fixed in PFA and immunostained for MERS-CoV N protein and actin. Virus entry was analyzed with confocal microscopy. The data shown are representative of three independent experiments, each performed in triplicate. * P

    Article Snippet: To visualize MERS-CoV particles, cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml; Thermo Fisher Scientific, Poland).

    Techniques: Incubation, Confocal Microscopy

    Single-dose vaccination with ChAdOx1 MERS protects rhesus macaques against bronchointerstitial pneumonia caused by MERS-CoV challenge. Rhesus macaques were vaccinated with a prime-boost or prime-only regimen of ChAdOx1 MERS, or with ChAdOx1 GFP and challenged with MERS-CoV. (A) Ventrodorsal thoracic radiographs collected on 6 DPI. A marker (R) indicates right side of animal. No pathologic changes were observed in animals vaccinated with ChAdOx1 MERS via a prime-boost or prime-only regimen. Animal vaccinated with ChAdOx1 GFP shows focally extensive area of increased pulmonary opacity and deviation of the cardiac silhouette, highlighted in the circle located in the middle and caudal lung lobes. (B) Thoracic radiographs of each animal were scored per lung lobe resulting in a maximum score of 18. Values were averaged per group per day, shown is mean with standard deviation. See Table S2 for more details. (C) Gross pathology of lungs shows no pathologic changes in ChAdOx1 MERS vaccinated animals, and focally extensive areas of consolidation in left cranial, middle and caudal lung lobes in control animals (asterisks). (D) Gross lung lesions were scored for each lung lobe, ventral and dorsal. Values were averaged per group, shown is mean with standard deviation. (E) Lung tissue sections were stained with hematoxylin and eosin. Moderate numbers of lymphocyte accumulation around pulmonary arterioles (asterisks), and mild thickening of alveolar septae by lymphocytes and macrophages (arrow) in lung tissue of animals vaccinated with ChAdOx1 MERS. Marked bronchointerstitial pneumonia with abundant pulmonary edema and fibrin (asterisks), type II pneumocyte hyperplasia (arrow), and increased numbers of alveolar macrophages (arrowhead) in lung tissue of control animals. Magnification = 200x. (F) Lung to body weight ratio was determined for all animals at necropsy. Shown is mean with standard deviation. (G) Lung tissue sections were stained with antibody against MERS-CoV antigen, which is visible as a red-brown staining. No immunoreactivity was found in ChAdOx1 MERS vaccinated animals, whereas multifocal immunoreactivity of type I and II pneumocytes could be found in lung tissue of ChAdOx1 GFP vaccinated animals. (H) Lung tissue sections were scored on severity of lesions (0=no lesions, 1=1-10%, 2=11-25%, 3=26-50%, 4=51-75%, 5=76-100%) and averaged per group. Shown is mean with standard deviation. A = bronchointerstitial pneumonia; B = type II pneumocyte hyperplasia; C = Hemorrhages, edema, fibrin deposits. Statistical significance between groups was determined via two-tailed unpaired Student’s t-test. * = p-value

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: Single-dose vaccination with ChAdOx1 MERS protects rhesus macaques against bronchointerstitial pneumonia caused by MERS-CoV challenge. Rhesus macaques were vaccinated with a prime-boost or prime-only regimen of ChAdOx1 MERS, or with ChAdOx1 GFP and challenged with MERS-CoV. (A) Ventrodorsal thoracic radiographs collected on 6 DPI. A marker (R) indicates right side of animal. No pathologic changes were observed in animals vaccinated with ChAdOx1 MERS via a prime-boost or prime-only regimen. Animal vaccinated with ChAdOx1 GFP shows focally extensive area of increased pulmonary opacity and deviation of the cardiac silhouette, highlighted in the circle located in the middle and caudal lung lobes. (B) Thoracic radiographs of each animal were scored per lung lobe resulting in a maximum score of 18. Values were averaged per group per day, shown is mean with standard deviation. See Table S2 for more details. (C) Gross pathology of lungs shows no pathologic changes in ChAdOx1 MERS vaccinated animals, and focally extensive areas of consolidation in left cranial, middle and caudal lung lobes in control animals (asterisks). (D) Gross lung lesions were scored for each lung lobe, ventral and dorsal. Values were averaged per group, shown is mean with standard deviation. (E) Lung tissue sections were stained with hematoxylin and eosin. Moderate numbers of lymphocyte accumulation around pulmonary arterioles (asterisks), and mild thickening of alveolar septae by lymphocytes and macrophages (arrow) in lung tissue of animals vaccinated with ChAdOx1 MERS. Marked bronchointerstitial pneumonia with abundant pulmonary edema and fibrin (asterisks), type II pneumocyte hyperplasia (arrow), and increased numbers of alveolar macrophages (arrowhead) in lung tissue of control animals. Magnification = 200x. (F) Lung to body weight ratio was determined for all animals at necropsy. Shown is mean with standard deviation. (G) Lung tissue sections were stained with antibody against MERS-CoV antigen, which is visible as a red-brown staining. No immunoreactivity was found in ChAdOx1 MERS vaccinated animals, whereas multifocal immunoreactivity of type I and II pneumocytes could be found in lung tissue of ChAdOx1 GFP vaccinated animals. (H) Lung tissue sections were scored on severity of lesions (0=no lesions, 1=1-10%, 2=11-25%, 3=26-50%, 4=51-75%, 5=76-100%) and averaged per group. Shown is mean with standard deviation. A = bronchointerstitial pneumonia; B = type II pneumocyte hyperplasia; C = Hemorrhages, edema, fibrin deposits. Statistical significance between groups was determined via two-tailed unpaired Student’s t-test. * = p-value

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Marker, Standard Deviation, Staining, Two Tailed Test

    ChAdOx1 MERS provides cross-protection against different MERS-CoV strains in the mouse model. (A) Survival curves of ChAdOx1 MERS vaccinated (solid line) and ChAdOx1 GFP vaccinated (dashed line) hDPP4 mice challenged with MERS-CoV. (B) Infectious virus titers in lung tissue collected on 3 DPI from hDPP4 mice challenged with MERS-CoV. Shown is mean titer with standard deviation.

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: ChAdOx1 MERS provides cross-protection against different MERS-CoV strains in the mouse model. (A) Survival curves of ChAdOx1 MERS vaccinated (solid line) and ChAdOx1 GFP vaccinated (dashed line) hDPP4 mice challenged with MERS-CoV. (B) Infectious virus titers in lung tissue collected on 3 DPI from hDPP4 mice challenged with MERS-CoV. Shown is mean titer with standard deviation.

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Mouse Assay, Standard Deviation

    Maximum likelihood phylogeny of all published full-length MERS-CoV nucleotide sequences. Strains highlighted in green: human isolates used in this study; strains highlighted in blue: camel isolates used in this study; strain highlighted in red: ChAdOx1 MERS strain.

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: Maximum likelihood phylogeny of all published full-length MERS-CoV nucleotide sequences. Strains highlighted in green: human isolates used in this study; strains highlighted in blue: camel isolates used in this study; strain highlighted in red: ChAdOx1 MERS strain.

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques:

    Vaccination of rhesus macaques with ChAdOx1 MERS elicits a humoral immune response. Serum samples were collected from non-human primates at times of vaccination (−56 DPI and -28 DPI), 14 days later and at challenge. (A) Overview of experimental timeline. V-M = vaccination with ChAdOx1 MERS; V-G = vaccination with ChAdOx1 GFP; E = exam; C = challenge and exam; N = exam and necropsy (B) Two-fold serial-diluted serum samples were tested for MERS-CoV S-specific antibodies using ELISA. (C) ELISA-positive two-fold serial-diluted serum samples were tested for neutralizing antibodies against MERS-CoV in VeroE6 cells. Line = geometric mean, dotted line = limit of detection. Statistical significance between -28 DPI and -14 DPI in the prime-boost group was determined via one-tailed paired Student’s t-test. ** = p-value

    Journal: bioRxiv

    Article Title: A single dose of ChAdOx1 MERS provides broad protective immunity against a variety of MERS-CoV strains

    doi: 10.1101/2020.04.13.036293

    Figure Lengend Snippet: Vaccination of rhesus macaques with ChAdOx1 MERS elicits a humoral immune response. Serum samples were collected from non-human primates at times of vaccination (−56 DPI and -28 DPI), 14 days later and at challenge. (A) Overview of experimental timeline. V-M = vaccination with ChAdOx1 MERS; V-G = vaccination with ChAdOx1 GFP; E = exam; C = challenge and exam; N = exam and necropsy (B) Two-fold serial-diluted serum samples were tested for MERS-CoV S-specific antibodies using ELISA. (C) ELISA-positive two-fold serial-diluted serum samples were tested for neutralizing antibodies against MERS-CoV in VeroE6 cells. Line = geometric mean, dotted line = limit of detection. Statistical significance between -28 DPI and -14 DPI in the prime-boost group was determined via one-tailed paired Student’s t-test. ** = p-value

    Article Snippet: Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000.

    Techniques: Enzyme-linked Immunosorbent Assay, One-tailed Test

    IHC detection of virus antigen expression in mouse tissue after challenge with MERS-CoV. Lung (A–C) and trachea (D–F) sections were assessed using rabbit polyclonal antibody to MERS-CoV nucleoprotein (NP) 3 days after the MERS-CoV challenge. The dark purple spot marked the inflammatory cell infiltration, and the brown particle marked the antigen of MERS-CoV. The MERS-CoV was located mainly in the trachea. Additionally, the lung tissue showed MERS-CoV expression in all immunized groups.

    Journal: Vaccine

    Article Title: The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection

    doi: 10.1016/j.vaccine.2016.11.064

    Figure Lengend Snippet: IHC detection of virus antigen expression in mouse tissue after challenge with MERS-CoV. Lung (A–C) and trachea (D–F) sections were assessed using rabbit polyclonal antibody to MERS-CoV nucleoprotein (NP) 3 days after the MERS-CoV challenge. The dark purple spot marked the inflammatory cell infiltration, and the brown particle marked the antigen of MERS-CoV. The MERS-CoV was located mainly in the trachea. Additionally, the lung tissue showed MERS-CoV expression in all immunized groups.

    Article Snippet: Rabbit-serum-derived polyclonal antibody against nucleoprotein (cat: 100213-RP02; Sino Biological Inc., Beijing, CHN) was incubated with the sections at 1:1000 dilution; goat anti-rabbit (cat: pv-9001; ZSGB-Bio, Beijing, CHN) secondary antibody was used at 1:2000, and sections were evaluated using light microscopy.

    Techniques: Immunohistochemistry, Expressing

    rNTD or rRBD vaccination reduced respiratory tract pathology in mice after MERS-CoV challenge. Representative results of hematoxylin-eosin (HE) staining in the lung (A – C) and trachea (D – F) of mock-treated or immunized mice. Severe lesions including the loss of pulmonary alveolus (represented by the white vacuole) and diffuse inflammatory cell infiltration (represented by the dark purple point) are shown (figure A). In contrast, milder lesions were observed among mice immunized with rRBD (figure B) or NTD (figure C), as the pulmonary alveolus was highly visible with less inflammatory cell infiltration. Inflammatory cell infiltration and impaired epithelium of the tunica mucosa bronchiorum were seen in the mock group (D). rRBD (E) or rNTD (F) alleviated the pathologic damage in the trachea of immunized mice. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Vaccine

    Article Title: The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection

    doi: 10.1016/j.vaccine.2016.11.064

    Figure Lengend Snippet: rNTD or rRBD vaccination reduced respiratory tract pathology in mice after MERS-CoV challenge. Representative results of hematoxylin-eosin (HE) staining in the lung (A – C) and trachea (D – F) of mock-treated or immunized mice. Severe lesions including the loss of pulmonary alveolus (represented by the white vacuole) and diffuse inflammatory cell infiltration (represented by the dark purple point) are shown (figure A). In contrast, milder lesions were observed among mice immunized with rRBD (figure B) or NTD (figure C), as the pulmonary alveolus was highly visible with less inflammatory cell infiltration. Inflammatory cell infiltration and impaired epithelium of the tunica mucosa bronchiorum were seen in the mock group (D). rRBD (E) or rNTD (F) alleviated the pathologic damage in the trachea of immunized mice. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Rabbit-serum-derived polyclonal antibody against nucleoprotein (cat: 100213-RP02; Sino Biological Inc., Beijing, CHN) was incubated with the sections at 1:1000 dilution; goat anti-rabbit (cat: pv-9001; ZSGB-Bio, Beijing, CHN) secondary antibody was used at 1:2000, and sections were evaluated using light microscopy.

    Techniques: Mouse Assay, Staining

    Description of the N-terminal domain (NTD) immunogen and vaccination schedule. (A) The location of the NTD protein on the Middle East respiratory syndrome coronavirus MERS-CoV spike (S) protein. The recombinant (r)NTD protein consists of 336 amino acid (aa) residues (18–353) of S protein. A gp67 signal peptide (SP) was added to the N terminus for expression of the rNTD protein. (B) Purified rNTD protein detected by SDS-PAGE (left) and Western blot (right). The purified rNTD protein was separated by a 10% SDS-PAGE and stained with 0.25% Coomassie brilliant blue. Anti-NTD polyclonal antibody and infrared ray-labeled secondary antibody were used for the Western blot assay. Lane 1: protein molecular weight marker; lane 2: purified rNTD protein. (C). Vaccination schedule and detection. Mice received three vaccinations consisting of 5 or 10 μg of rNTD protein combined with adjuvants at 4-week intervals. Sera were collected at the indicated times to analyze the humoral immune response. Six mice from each group were sacrificed 2 weeks after the last immunization. The spleens were harvested for enzyme-linked immunospot (ELISpot), intracellular cytokine staining (ICS), and cytometric bead array (CBA) assays. In parallel experiments, the remaining mice were challenged with MERS-CoV to detect the protective effect elicited by the rNTD protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Vaccine

    Article Title: The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection

    doi: 10.1016/j.vaccine.2016.11.064

    Figure Lengend Snippet: Description of the N-terminal domain (NTD) immunogen and vaccination schedule. (A) The location of the NTD protein on the Middle East respiratory syndrome coronavirus MERS-CoV spike (S) protein. The recombinant (r)NTD protein consists of 336 amino acid (aa) residues (18–353) of S protein. A gp67 signal peptide (SP) was added to the N terminus for expression of the rNTD protein. (B) Purified rNTD protein detected by SDS-PAGE (left) and Western blot (right). The purified rNTD protein was separated by a 10% SDS-PAGE and stained with 0.25% Coomassie brilliant blue. Anti-NTD polyclonal antibody and infrared ray-labeled secondary antibody were used for the Western blot assay. Lane 1: protein molecular weight marker; lane 2: purified rNTD protein. (C). Vaccination schedule and detection. Mice received three vaccinations consisting of 5 or 10 μg of rNTD protein combined with adjuvants at 4-week intervals. Sera were collected at the indicated times to analyze the humoral immune response. Six mice from each group were sacrificed 2 weeks after the last immunization. The spleens were harvested for enzyme-linked immunospot (ELISpot), intracellular cytokine staining (ICS), and cytometric bead array (CBA) assays. In parallel experiments, the remaining mice were challenged with MERS-CoV to detect the protective effect elicited by the rNTD protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Rabbit-serum-derived polyclonal antibody against nucleoprotein (cat: 100213-RP02; Sino Biological Inc., Beijing, CHN) was incubated with the sections at 1:1000 dilution; goat anti-rabbit (cat: pv-9001; ZSGB-Bio, Beijing, CHN) secondary antibody was used at 1:2000, and sections were evaluated using light microscopy.

    Techniques: Recombinant, Expressing, Purification, SDS Page, Western Blot, Staining, Labeling, Molecular Weight, Marker, Mouse Assay, Enzyme-linked Immunospot, Crocin Bleaching Assay