mouse anti krt6a  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Alteration of Oral and Perioral Soft Tissue in Mice following Incisor Tooth Extraction

doi: 10.3390/ijms23062987

Figure Lengend Snippet: ( A ) Experimental procedure for the proteomic investigation. Histogram of protein counts grouped according to log2 protein ratios. In total, 1473 proteins were detected in the two groups using high-sensitivity liquid chromatography–tandem mass spectrometry. ( B ) Volcano plot of the statistical significance of differences (Student’s t -test p -value) as a function of the average protein ratios. Fifteen proteins were enriched in UH mouse lips (orange), while 14 were enriched in NH mouse lips (green). Heatmap illustrating quantitative alterations of representative proteins. Enrichment of S100a8, S100a9, keratin 6a (KRT6a), keratin 6b (KRT6b), keratin 8 (KRT8), and keratin 16 (KRT16) expression was detected in UH mouse lips.

Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies: mouse anti-keratin 6a (KRT6a) antibody (1:200 dilution; BioLegend, San Diego, CA, USA), rabbit anti-keratin 6b (KRT6b) antibody (1:200 dilution; Merck Millipore, Waltham, MA, USA), rabbit anti-S100A8 antibody (1:200 dilution; Abcam, Cambridge, UK), and rabbit anti-S100A9 antibody (1:200 dilution; Novus Biologicals, Minneapolis, MN, USA).

Techniques: Liquid Chromatography, Mass Spectrometry, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Alteration of Oral and Perioral Soft Tissue in Mice following Incisor Tooth Extraction

doi: 10.3390/ijms23062987

Figure Lengend Snippet: An upper lip comparison between the non-extraction control (NH) and tooth loss (UH) groups: ( A ) Histological studies of upper lips. The upper lip was more strongly protruded into the oral cavity in UH mice than in NH mice (Panels c–g). ( B ) High-magnification views of the upper lips. After extracting an incisor, the thickness of the upper lip’s epithelium was increased (Panels a and g) (Panel m; ** p < 0.01). The cross-sectional area of the orbicularis oris muscle (arrowheads) was reduced (Panels b and h) (Panel n; ** p < 0.01). KRT6A/6B fluorescence intensity in the granular layer was lower in UH mice than in NH mice (Panels c, d, i, j, o, and p). S100A8/A9 density was significantly higher in UH mice than in NH mice (Panels e, f, k, and l) (Panels q and r; ** p < 0.01).

Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies: mouse anti-keratin 6a (KRT6a) antibody (1:200 dilution; BioLegend, San Diego, CA, USA), rabbit anti-keratin 6b (KRT6b) antibody (1:200 dilution; Merck Millipore, Waltham, MA, USA), rabbit anti-S100A8 antibody (1:200 dilution; Abcam, Cambridge, UK), and rabbit anti-S100A9 antibody (1:200 dilution; Novus Biologicals, Minneapolis, MN, USA).

Techniques: Comparison, Extraction, Control, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Alteration of Oral and Perioral Soft Tissue in Mice following Incisor Tooth Extraction

doi: 10.3390/ijms23062987

Figure Lengend Snippet: Soft-diet feeding affects lip morphology: ( A ) Body weight was significantly higher in the soft-diet feeding (US) group than in the hard-diet feeding (UH) group (Panel b; * p < 0.05, *** p < 0.001). Because the thickness and area of the lamina propria were smaller in US mice than in UH mice (Panels c and d) (Panels e and f; * p < 0.05), lip protrusion into the oral cavity was less severe in US mice. ( B ) The thickness of the epithelium and area of the muscle did not differ between the groups (Panels a, f, k, and l). The fluorescence intensity of KRT6A/6B in the granular layer did not differ between UH and US mice (Panels m and n). The density of S100A8/A9 was not different between the groups (Panels d, e, i, j, o, and p).

Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies: mouse anti-keratin 6a (KRT6a) antibody (1:200 dilution; BioLegend, San Diego, CA, USA), rabbit anti-keratin 6b (KRT6b) antibody (1:200 dilution; Merck Millipore, Waltham, MA, USA), rabbit anti-S100A8 antibody (1:200 dilution; Abcam, Cambridge, UK), and rabbit anti-S100A9 antibody (1:200 dilution; Novus Biologicals, Minneapolis, MN, USA).

Techniques: Fluorescence

Journal: Aging (Albany NY)

Article Title: Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma

doi: 10.18632/aging.104091

Figure Lengend Snippet: KRT6A may participate in regulating TAMs in PDAC. ( A ) Venn diagram of common DEGs between PDAC and ANT and between the M0-high group and the M0-low group. ( B ) Correlation of gene expression between KRT6A and various TAM-related genes in PDAC. ( C ) Heatmap of TAM-related gene expression patterns of the KRT6A-high group and KRT6A-low group in PDAC. ( D ) KEGG (left) and GO (right) pathway enrichment analyses of DEGs between the KRT6A-high group and the KRT6A-low group. ( E , F ) Protein-protein interaction network related to KRT6A using STRING ( E ) and GeneMANIA ( F ). ( G ) Survival analysis of the KRT6A-high group and the KRT6A-low group in TCGA PAAD data (left) and GSE57495 (right).

Article Snippet: For immunofluorescence staining, frozen tissue section samples of PDAC and ANT were incubated with a mixture of rabbit anti-ITGAM (Proteintech; 1:50) and mouse anti-KRT6A (Proteintech; 1:50) antibodies overnight at 4°C after dewaxing, hydrating, antigen retrieval and blocking, followed by incubation with a mixture of Alexa Fluor 488 or Alexa Fluor 555 fluorescence-conjugated secondary antibodies (Abcam; 1: 200) for 1 hour and DAPI for 10 minutes.

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma

doi: 10.18632/aging.104091

Figure Lengend Snippet: Immunofluorescence and immunohistochemistry (IHC) staining by integrating KRT6A and ITGAM expression. ( A ) Immunofluorescence staining of KRT6A (green) and ITGAM (red) in PDAC and ANT frozen tissue sections (100×). ( B ) Pearson's correlation of KRT6A and ITGAM expressing cells in PDAC and ANT. ( C – F ) IHC staining of KRT6A (c) and ITGAM (e) in PDAC and ANT (200×). Column charts were shown in ( D ) for KRT6A and ( F ) for ITGAM. *p<0.001.

Article Snippet: For immunofluorescence staining, frozen tissue section samples of PDAC and ANT were incubated with a mixture of rabbit anti-ITGAM (Proteintech; 1:50) and mouse anti-KRT6A (Proteintech; 1:50) antibodies overnight at 4°C after dewaxing, hydrating, antigen retrieval and blocking, followed by incubation with a mixture of Alexa Fluor 488 or Alexa Fluor 555 fluorescence-conjugated secondary antibodies (Abcam; 1: 200) for 1 hour and DAPI for 10 minutes.

Techniques: Immunofluorescence, Immunohistochemistry, Expressing, Staining

Journal: Aging (Albany NY)

Article Title: Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma

doi: 10.18632/aging.104091

Figure Lengend Snippet: The molecular mechanism by which KRT6A regulates TAMs. ( A ) CEMiTool was used to find gene modules affecting TAMs through KRT6A by dividing TCGA PAAD mRNA-seq data into five groups by the level of KRT6A expression: Grade 0 (G0), Grade 1 (G1), Grade 2 (G2), Grade 3 (G3), and Grade 4 (G4), 6A expression where G0 expressed the lowest amounts of KRT6A (average RPKM = 0) and G4 expressed the highest amounts of KRT6A (average RPKM = 193.24). ( B ) Expression of a gene module (Module 1) in each PDAC patient. ( C ) Major genes and the number of genes in each module. ( D ) Transcription factor PPI network and pathway enrichment of genes in Module 1. ( E ) Cell type enrichment of genes in Module 1.

Article Snippet: For immunofluorescence staining, frozen tissue section samples of PDAC and ANT were incubated with a mixture of rabbit anti-ITGAM (Proteintech; 1:50) and mouse anti-KRT6A (Proteintech; 1:50) antibodies overnight at 4°C after dewaxing, hydrating, antigen retrieval and blocking, followed by incubation with a mixture of Alexa Fluor 488 or Alexa Fluor 555 fluorescence-conjugated secondary antibodies (Abcam; 1: 200) for 1 hour and DAPI for 10 minutes.

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Alteration of tumor-associated macrophage subtypes mediated by KRT6A in pancreatic ductal adenocarcinoma

doi: 10.18632/aging.104091

Figure Lengend Snippet: Genes that were expressed similarly to KRT6A in PDAC were identified using the STEM tool.

Article Snippet: For immunofluorescence staining, frozen tissue section samples of PDAC and ANT were incubated with a mixture of rabbit anti-ITGAM (Proteintech; 1:50) and mouse anti-KRT6A (Proteintech; 1:50) antibodies overnight at 4°C after dewaxing, hydrating, antigen retrieval and blocking, followed by incubation with a mixture of Alexa Fluor 488 or Alexa Fluor 555 fluorescence-conjugated secondary antibodies (Abcam; 1: 200) for 1 hour and DAPI for 10 minutes.

Techniques: