Journal: Oncology Reports

Article Title: Exosomal PD‑L1 promotes the formation of an immunosuppressive microenvironment in gastric diffuse large B‑cell lymphoma

doi: 10.3892/or.2023.8525

Figure Lengend Snippet: Protein expression levels of protein markers of B cells and the immune microenvironment in tissue specimens of gastric diffuse large B-cell lymphoma. (A and B) Immunohistochemistry images of protein expression levels of the proliferative B-cell markers CD20, CD79a, CD5, CD10, Ki67 and PD-L1 as well as the immune microenvironmental markers CD4, CD8 and PD-1 in two representative tissue specimens of GDLBCL. Scale bar=50 µm. PD-L1, programmed death-ligand 1.

Article Snippet: The antibodies used for IHC staining were as follows: Rabbit anti-PD-1 (1:200, clone D4W2J, cat. no. 86163S, Cell Signaling Technologies, Inc.), rabbit anti-PD-L1 (1:500, 17952-1-AP, Proteintech Group, Inc.), mouse anti-CD8 (1:5,000, clone 1G2B10, cat. no. 66868-1-Ig, Proteintech Group, Inc.), rabbit anti-CD5 (1:800, clone E8X3S, cat. no. 39300S, Cell Signaling Technologies, Inc.), rabbit anti-CD10 (1:500, clone E5P7S, cat. no. 65534S, Cell Signaling Technologies, Inc.), rabbit anti-CD20 (1:200, clone E7B7T, cat. no. 48750S, Cell Signaling Technologies, Inc.), rabbit anti-CD79a (1:250, clone D1X5C, cat. no. 13333S, Cell Signaling Technologies, Inc.), mouse anti-Ki67 (1:2,000, clone 8D5, cat. no. 9449S, Cell Signaling Technologies, Inc.), goat anti-mouse IgG (HRP-linked, 1:2,000, cat. no. AP124P, MilliporeSigma), and goat anti-rabbit IgG (HRP-linked, 1:2,000, cat. no. AP132P, MilliporeSigma).

Techniques: Expressing, Immunohistochemistry

Journal: Oncology Reports

Article Title: Exosomal PD‑L1 promotes the formation of an immunosuppressive microenvironment in gastric diffuse large B‑cell lymphoma

doi: 10.3892/or.2023.8525

Figure Lengend Snippet: The relationship between the protein level of PD-L1 in plasma exosomes and the immune microenvironment of gastric diffuse large B-cell lymphoma.

Article Snippet: The antibodies used for IHC staining were as follows: Rabbit anti-PD-1 (1:200, clone D4W2J, cat. no. 86163S, Cell Signaling Technologies, Inc.), rabbit anti-PD-L1 (1:500, 17952-1-AP, Proteintech Group, Inc.), mouse anti-CD8 (1:5,000, clone 1G2B10, cat. no. 66868-1-Ig, Proteintech Group, Inc.), rabbit anti-CD5 (1:800, clone E8X3S, cat. no. 39300S, Cell Signaling Technologies, Inc.), rabbit anti-CD10 (1:500, clone E5P7S, cat. no. 65534S, Cell Signaling Technologies, Inc.), rabbit anti-CD20 (1:200, clone E7B7T, cat. no. 48750S, Cell Signaling Technologies, Inc.), rabbit anti-CD79a (1:250, clone D1X5C, cat. no. 13333S, Cell Signaling Technologies, Inc.), mouse anti-Ki67 (1:2,000, clone 8D5, cat. no. 9449S, Cell Signaling Technologies, Inc.), goat anti-mouse IgG (HRP-linked, 1:2,000, cat. no. AP124P, MilliporeSigma), and goat anti-rabbit IgG (HRP-linked, 1:2,000, cat. no. AP132P, MilliporeSigma).

Techniques:

Journal: bioRxiv

Article Title: Targeting Ras signaling excitability in cancer cells through combined inhibition of FAK and PI3K

doi: 10.1101/2023.06.12.544386

Figure Lengend Snippet: (A) Proliferation of cells treated with indicated concentrations of PF-04691502 and VS-6063 tracked using the RealTime-Glo MT Cell Viability Assay. (B) Flow cytometry analysis of caspase-3 and Ki-67 staining of HeLa cells treated with indicated concentrations of PF-04691502 and VS-6063 for 8h, 12h, and 24h. (C) Example of cell cycle phase determination by Ki-67 and DAPI staining. In (B) and (C), red boxes indicate M phase cells. (D-E) Quantification of M phase (red boxes) and caspase-3 (+) cells in (B). The numbers represent mean ± SEM of n=3 experiments.

Article Snippet: The cells were then incubated with DAPI (10 mg/mL, 1:1000), mouse anti-human Ki-67 (Cell Signaling #9449, 1:400), and rabbit anti-human cleaved caspase 3 antibodies (Cell Signaling #9661, 1:800) at room temperature for 1 hour.

Techniques: Viability Assay, Flow Cytometry, Staining

Journal: iScience

Article Title: Tumor-suppressive role of the musculoaponeurotic fibrosarcoma gene in colorectal cancer

doi: 10.1016/j.isci.2023.106478

Figure Lengend Snippet: Key resources table

Article Snippet: They were incubated overnight at 4°C with anti-mouse monoclonal rabbit antibody against Ki-67 (#12202, Cell Signaling Technology) at dilutions of 1:200.

Techniques: Recombinant, Transfection, DNA Extraction, CCK-8 Assay, SYBR Green Assay, Plasmid Preparation, Amplification, Bradford Protein Assay, Microarray, RNA Sequencing Assay, Negative Control, Expressing, Sequencing, Software, CRISPR

Journal: Cell reports

Article Title: Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration

doi: 10.1016/j.celrep.2022.110473

Figure Lengend Snippet: KEY RESOURCES TABLE Primary and secondary antibodies

Article Snippet: LTL was purchased from Vector laboratories (cat. no. NC0127927). . Antibodies: Anti-pSTAT3 Tyrosine 705 (cat. no. 9145) and anti-STAT3 (cat. no. 9139), anti-Ki67 (cat. no. 9449), anti-F4/80 (cat. no. 30325), pH2AX (cat. no. 9718), pSMAD2 (cat. no. 18338) were purchased from Cell Signaling Technology (Danvers, MA).

Techniques: