human tlr2 antibody  (Hycult Biotech)


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    Structured Review

    Hycult Biotech human tlr2 antibody
    Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with <t>TLR2</t> agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001
    Human Tlr2 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tlr2 antibody/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tlr2 antibody - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma"

    Article Title: Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma

    Journal: Medical Oncology (Northwood, London, England)

    doi: 10.1007/s12032-022-01664-5

    Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with TLR2 agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001
    Figure Legend Snippet: Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with TLR2 agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001

    Techniques Used: Enzyme-linked Immunosorbent Assay

    The effect of TLR2 neutralising antibody on IL-6 production in SCC-4 cells. ( A ) DOK and ( B ) SCC-4 cells were untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam2CSK4 (0.2 μg/ml) and Pam3CSK4 (0.2 μg/ml) for 24 h. Culture supernatants were analysed for IL-6 secretion by ELISA. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Dunnett’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001
    Figure Legend Snippet: The effect of TLR2 neutralising antibody on IL-6 production in SCC-4 cells. ( A ) DOK and ( B ) SCC-4 cells were untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam2CSK4 (0.2 μg/ml) and Pam3CSK4 (0.2 μg/ml) for 24 h. Culture supernatants were analysed for IL-6 secretion by ELISA. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Dunnett’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Techniques Used: Enzyme-linked Immunosorbent Assay

    TLR2 and TLR6 expression levels in SCC-4 cells and DOK cells. DOK and SCC-4 cells were left untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam3CSK4 (0.05 μg/ml) or Pam2CSK4 (0.05 μg/ml) for 24 h. Lysates were collected and samples were run on 12% SDS-PAGE gel. Western Blot were probed with anti-TLR2 and anti-TLR6 antibodies with anti-GAPDH used as a loading control. Experiment was done in triplicate. Densitometric analysis was performed using image lab software on TLR2, TLR6 and GAPDH blots. Sample blots for ( A ) DOK and ( B ) SCC-4 and collated densitometry data for ( C ) TLR2 and ( D ) TLR6 are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using two-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001
    Figure Legend Snippet: TLR2 and TLR6 expression levels in SCC-4 cells and DOK cells. DOK and SCC-4 cells were left untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam3CSK4 (0.05 μg/ml) or Pam2CSK4 (0.05 μg/ml) for 24 h. Lysates were collected and samples were run on 12% SDS-PAGE gel. Western Blot were probed with anti-TLR2 and anti-TLR6 antibodies with anti-GAPDH used as a loading control. Experiment was done in triplicate. Densitometric analysis was performed using image lab software on TLR2, TLR6 and GAPDH blots. Sample blots for ( A ) DOK and ( B ) SCC-4 and collated densitometry data for ( C ) TLR2 and ( D ) TLR6 are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using two-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Techniques Used: Expressing, SDS Page, Western Blot, Software

    Survival rate in anoikis assays DOK and SCC-4 cells treated with rhIL-6, IL-6 neutralising antibody, IL-6Ra antibody, IL-6Ra + rhIL-6, Pam2CSK4, anti-TLR2 neutralising antibody + Pam2CSK4, and anti-TLR2 neutralising antibody + Pam2CSK4 for 24 h. Tissue culture plates were coated with 200 μl poly-(hydroxyethyl methacrylic) acid (poly-HEMA) thus inhibiting the ability of the cells to attach to the tissue culture plastic. ( A ) DOK and ( B ) SCC-4 cells were then seeded at 100,000 cells/well and allowed to attach. For those cells to be treated with 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, 40 μg/ml IL-6Ra antibody, 40 μg/ml IL-6Ra + 30 ng/ml rhIL-6, 0.2 μg/ml Pam2CSK4, and 10 μg/ml anti-TLR2 neutralising antibody + 0.2 μg/ml Pam2CSK4, complete medium was used as control, as well as medium containing 10 μg/ml isotype IgG antibody. After 24 h, 50 μl of alamarBlue ® dye was added to each well and incubated again at 37 °C/5% CO 2 for 3.5 h. SCC-4 cells demonstrated significant resistance to anoikis (% survival) when treated with 30 ng/ml rhIL-6 and 0.2 μg/ml Pam2CSK4 compared to the control group. There is a100% survival of adherent cells in the uncoated plates. Fluorescence was measured using a SpectraMax Gemini plate reader at excitation wavelength 544 nm and emission wavelength 590 nm. Results were plotted using Graphpad prism 8. Results represent at least three experiments performed in duplicate (mean ± SEM ( n )). Statistical analysis was performed one-way ANOVA with a post hoc Tukey’s test to compare mean values among the groups, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001
    Figure Legend Snippet: Survival rate in anoikis assays DOK and SCC-4 cells treated with rhIL-6, IL-6 neutralising antibody, IL-6Ra antibody, IL-6Ra + rhIL-6, Pam2CSK4, anti-TLR2 neutralising antibody + Pam2CSK4, and anti-TLR2 neutralising antibody + Pam2CSK4 for 24 h. Tissue culture plates were coated with 200 μl poly-(hydroxyethyl methacrylic) acid (poly-HEMA) thus inhibiting the ability of the cells to attach to the tissue culture plastic. ( A ) DOK and ( B ) SCC-4 cells were then seeded at 100,000 cells/well and allowed to attach. For those cells to be treated with 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, 40 μg/ml IL-6Ra antibody, 40 μg/ml IL-6Ra + 30 ng/ml rhIL-6, 0.2 μg/ml Pam2CSK4, and 10 μg/ml anti-TLR2 neutralising antibody + 0.2 μg/ml Pam2CSK4, complete medium was used as control, as well as medium containing 10 μg/ml isotype IgG antibody. After 24 h, 50 μl of alamarBlue ® dye was added to each well and incubated again at 37 °C/5% CO 2 for 3.5 h. SCC-4 cells demonstrated significant resistance to anoikis (% survival) when treated with 30 ng/ml rhIL-6 and 0.2 μg/ml Pam2CSK4 compared to the control group. There is a100% survival of adherent cells in the uncoated plates. Fluorescence was measured using a SpectraMax Gemini plate reader at excitation wavelength 544 nm and emission wavelength 590 nm. Results were plotted using Graphpad prism 8. Results represent at least three experiments performed in duplicate (mean ± SEM ( n )). Statistical analysis was performed one-way ANOVA with a post hoc Tukey’s test to compare mean values among the groups, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001

    Techniques Used: Incubation, Fluorescence

    mouse anti human tlr2 mab  (Hycult Biotech)


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    Structured Review

    Hycult Biotech mouse anti human tlr2 mab
    ( A ) <t>TLR2</t> expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.
    Mouse Anti Human Tlr2 Mab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human tlr2 mab/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human tlr2 mab - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "miR-1915 and miR-1225-5p Regulate the Expression of CD133, PAX2 and TLR2 in Adult Renal Progenitor Cells"

    Article Title: miR-1915 and miR-1225-5p Regulate the Expression of CD133, PAX2 and TLR2 in Adult Renal Progenitor Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0068296

    ( A ) TLR2 expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.
    Figure Legend Snippet: ( A ) TLR2 expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Transfection, Immunofluorescence, Staining

    human tlr2 antibody  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
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    Structured Review

    Hycult Biotech human tlr2 antibody
    Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with <t>TLR2</t> agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001
    Human Tlr2 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tlr2 antibody/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tlr2 antibody - by Bioz Stars, 2023-09
    93/100 stars

    Images

    1) Product Images from "Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma"

    Article Title: Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma

    Journal: Medical Oncology (Northwood, London, England)

    doi: 10.1007/s12032-022-01664-5

    Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with TLR2 agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001
    Figure Legend Snippet: Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with TLR2 agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001

    Techniques Used: Enzyme-linked Immunosorbent Assay

    The effect of TLR2 neutralising antibody on IL-6 production in SCC-4 cells. ( A ) DOK and ( B ) SCC-4 cells were untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam2CSK4 (0.2 μg/ml) and Pam3CSK4 (0.2 μg/ml) for 24 h. Culture supernatants were analysed for IL-6 secretion by ELISA. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Dunnett’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001
    Figure Legend Snippet: The effect of TLR2 neutralising antibody on IL-6 production in SCC-4 cells. ( A ) DOK and ( B ) SCC-4 cells were untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam2CSK4 (0.2 μg/ml) and Pam3CSK4 (0.2 μg/ml) for 24 h. Culture supernatants were analysed for IL-6 secretion by ELISA. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Dunnett’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Techniques Used: Enzyme-linked Immunosorbent Assay

    TLR2 and TLR6 expression levels in SCC-4 cells and DOK cells. DOK and SCC-4 cells were left untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam3CSK4 (0.05 μg/ml) or Pam2CSK4 (0.05 μg/ml) for 24 h. Lysates were collected and samples were run on 12% SDS-PAGE gel. Western Blot were probed with anti-TLR2 and anti-TLR6 antibodies with anti-GAPDH used as a loading control. Experiment was done in triplicate. Densitometric analysis was performed using image lab software on TLR2, TLR6 and GAPDH blots. Sample blots for ( A ) DOK and ( B ) SCC-4 and collated densitometry data for ( C ) TLR2 and ( D ) TLR6 are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using two-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001
    Figure Legend Snippet: TLR2 and TLR6 expression levels in SCC-4 cells and DOK cells. DOK and SCC-4 cells were left untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam3CSK4 (0.05 μg/ml) or Pam2CSK4 (0.05 μg/ml) for 24 h. Lysates were collected and samples were run on 12% SDS-PAGE gel. Western Blot were probed with anti-TLR2 and anti-TLR6 antibodies with anti-GAPDH used as a loading control. Experiment was done in triplicate. Densitometric analysis was performed using image lab software on TLR2, TLR6 and GAPDH blots. Sample blots for ( A ) DOK and ( B ) SCC-4 and collated densitometry data for ( C ) TLR2 and ( D ) TLR6 are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using two-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Techniques Used: Expressing, SDS Page, Western Blot, Software

    Survival rate in anoikis assays DOK and SCC-4 cells treated with rhIL-6, IL-6 neutralising antibody, IL-6Ra antibody, IL-6Ra + rhIL-6, Pam2CSK4, anti-TLR2 neutralising antibody + Pam2CSK4, and anti-TLR2 neutralising antibody + Pam2CSK4 for 24 h. Tissue culture plates were coated with 200 μl poly-(hydroxyethyl methacrylic) acid (poly-HEMA) thus inhibiting the ability of the cells to attach to the tissue culture plastic. ( A ) DOK and ( B ) SCC-4 cells were then seeded at 100,000 cells/well and allowed to attach. For those cells to be treated with 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, 40 μg/ml IL-6Ra antibody, 40 μg/ml IL-6Ra + 30 ng/ml rhIL-6, 0.2 μg/ml Pam2CSK4, and 10 μg/ml anti-TLR2 neutralising antibody + 0.2 μg/ml Pam2CSK4, complete medium was used as control, as well as medium containing 10 μg/ml isotype IgG antibody. After 24 h, 50 μl of alamarBlue ® dye was added to each well and incubated again at 37 °C/5% CO 2 for 3.5 h. SCC-4 cells demonstrated significant resistance to anoikis (% survival) when treated with 30 ng/ml rhIL-6 and 0.2 μg/ml Pam2CSK4 compared to the control group. There is a100% survival of adherent cells in the uncoated plates. Fluorescence was measured using a SpectraMax Gemini plate reader at excitation wavelength 544 nm and emission wavelength 590 nm. Results were plotted using Graphpad prism 8. Results represent at least three experiments performed in duplicate (mean ± SEM ( n )). Statistical analysis was performed one-way ANOVA with a post hoc Tukey’s test to compare mean values among the groups, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001
    Figure Legend Snippet: Survival rate in anoikis assays DOK and SCC-4 cells treated with rhIL-6, IL-6 neutralising antibody, IL-6Ra antibody, IL-6Ra + rhIL-6, Pam2CSK4, anti-TLR2 neutralising antibody + Pam2CSK4, and anti-TLR2 neutralising antibody + Pam2CSK4 for 24 h. Tissue culture plates were coated with 200 μl poly-(hydroxyethyl methacrylic) acid (poly-HEMA) thus inhibiting the ability of the cells to attach to the tissue culture plastic. ( A ) DOK and ( B ) SCC-4 cells were then seeded at 100,000 cells/well and allowed to attach. For those cells to be treated with 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, 40 μg/ml IL-6Ra antibody, 40 μg/ml IL-6Ra + 30 ng/ml rhIL-6, 0.2 μg/ml Pam2CSK4, and 10 μg/ml anti-TLR2 neutralising antibody + 0.2 μg/ml Pam2CSK4, complete medium was used as control, as well as medium containing 10 μg/ml isotype IgG antibody. After 24 h, 50 μl of alamarBlue ® dye was added to each well and incubated again at 37 °C/5% CO 2 for 3.5 h. SCC-4 cells demonstrated significant resistance to anoikis (% survival) when treated with 30 ng/ml rhIL-6 and 0.2 μg/ml Pam2CSK4 compared to the control group. There is a100% survival of adherent cells in the uncoated plates. Fluorescence was measured using a SpectraMax Gemini plate reader at excitation wavelength 544 nm and emission wavelength 590 nm. Results were plotted using Graphpad prism 8. Results represent at least three experiments performed in duplicate (mean ± SEM ( n )). Statistical analysis was performed one-way ANOVA with a post hoc Tukey’s test to compare mean values among the groups, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001

    Techniques Used: Incubation, Fluorescence

    t2 5 monoclonal anti tlr2 ab  (Hycult Biotech)


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    Hycult Biotech t2 5 monoclonal anti tlr2 ab
    T2 5 Monoclonal Anti Tlr2 Ab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t2 5 monoclonal anti tlr2 ab/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t2 5 monoclonal anti tlr2 ab - by Bioz Stars, 2023-09
    93/100 stars

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    t2 5  (Hycult Biotech)


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    Hycult Biotech t2 5
    T2 5, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t2 5/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t2 5 - by Bioz Stars, 2023-09
    93/100 stars

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    antibodies anti tlr2 mouse igg1 mab t2 5  (Hycult Biotech)


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    Hycult Biotech antibodies anti tlr2 mouse igg1 mab t2 5
    C57BL/6 mice or <t>TLR2</t> KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.
    Antibodies Anti Tlr2 Mouse Igg1 Mab T2 5, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies anti tlr2 mouse igg1 mab t2 5/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies anti tlr2 mouse igg1 mab t2 5 - by Bioz Stars, 2023-09
    93/100 stars

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    1) Product Images from "TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock"

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    Journal: Shock (Augusta, Ga.)

    doi: 10.1097/SHK.0000000000000650

    C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.
    Figure Legend Snippet: C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.

    Techniques Used:

    using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.
    Figure Legend Snippet: using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.

    Techniques Used: Generated

    C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.
    Figure Legend Snippet: C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.

    Techniques Used: Injection, In Vitro, Incubation, Translocation Assay

    (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm
    Figure Legend Snippet: (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm

    Techniques Used: Staining, Labeling, Marker

    C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)
    Figure Legend Snippet: C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)

    Techniques Used:

    anti tlr2 mouse igg1 mab t2 5  (Hycult Biotech)


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    Hycult Biotech anti tlr2 mouse igg1 mab t2 5
    C57BL/6 mice or <t>TLR2</t> KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.
    Anti Tlr2 Mouse Igg1 Mab T2 5, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tlr2 mouse igg1 mab t2 5/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti tlr2 mouse igg1 mab t2 5 - by Bioz Stars, 2023-09
    93/100 stars

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    1) Product Images from "TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock"

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    Journal: Shock (Augusta, Ga.)

    doi: 10.1097/SHK.0000000000000650

    C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.
    Figure Legend Snippet: C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.

    Techniques Used:

    using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.
    Figure Legend Snippet: using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.

    Techniques Used: Generated

    C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.
    Figure Legend Snippet: C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.

    Techniques Used: Injection, In Vitro, Incubation, Translocation Assay

    (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm
    Figure Legend Snippet: (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm

    Techniques Used: Staining, Labeling, Marker

    C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)
    Figure Legend Snippet: C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)

    Techniques Used:

    mouse anti human tlr2 mab  (Hycult Biotech)


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    Hycult Biotech mouse anti human tlr2 mab
    The repair induced by tARPCs was mediated by the Toll-like receptor-2. ( A ) BrdU cell proliferation assays showed that <t>TLR2</t> + tARPCs were able to induce the repair of cisplatin damaged RPTECs in the co-culture system. On the contrary, the TLR2 − tARPCs and the TLR2 + gARPCs did not change the proliferation rate of damaged cells. ( B ) Cisplatin damaged RPTECs showed a limited proliferation rate in co-culture with tARPCs when TLR2 on renal progenitors was neutralized by a specific TLR2 blocking antibody. Plots represent 5 independent experiments using ARPCs from 5 different subjects; **P < 0.005, ***P < 0.0005.
    Mouse Anti Human Tlr2 Mab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human tlr2 mab/product/Hycult Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human tlr2 mab - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration"

    Article Title: Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-08474-0

    The repair induced by tARPCs was mediated by the Toll-like receptor-2. ( A ) BrdU cell proliferation assays showed that TLR2 + tARPCs were able to induce the repair of cisplatin damaged RPTECs in the co-culture system. On the contrary, the TLR2 − tARPCs and the TLR2 + gARPCs did not change the proliferation rate of damaged cells. ( B ) Cisplatin damaged RPTECs showed a limited proliferation rate in co-culture with tARPCs when TLR2 on renal progenitors was neutralized by a specific TLR2 blocking antibody. Plots represent 5 independent experiments using ARPCs from 5 different subjects; **P < 0.005, ***P < 0.0005.
    Figure Legend Snippet: The repair induced by tARPCs was mediated by the Toll-like receptor-2. ( A ) BrdU cell proliferation assays showed that TLR2 + tARPCs were able to induce the repair of cisplatin damaged RPTECs in the co-culture system. On the contrary, the TLR2 − tARPCs and the TLR2 + gARPCs did not change the proliferation rate of damaged cells. ( B ) Cisplatin damaged RPTECs showed a limited proliferation rate in co-culture with tARPCs when TLR2 on renal progenitors was neutralized by a specific TLR2 blocking antibody. Plots represent 5 independent experiments using ARPCs from 5 different subjects; **P < 0.005, ***P < 0.0005.

    Techniques Used: Co-Culture Assay, Blocking Assay

    Difference of TLR2 expression in gARPCs and tARPCs following exposure to necrotic cell supernatants. Immunofluorescence experiments on ARPCs showing the TLR2 expression at basal conditions and after exposure to necrotic cell supernatants. ( A – D ) immunofluorescence showed the expression of TLR2 (green) in gARPCs, gARPCs + necrotic supernatant, tARPCs, and tARPCs + necrotic supernatans, respectively. ( E – H ) Immunofluorescence showed the expression of CD133 (red) in gARPCs, gARPCs + necrotic supernatant, tARPCs, and tARPCs + necrotic supernatants, respectively. ( I – L ) Double-label immunofluorescence showed the expression of TLR2 (green) and CD133 (red) in gARPCs, gARPCs + necrotic supernatant, tARPCs, and tARPCs + necrotic supernatant, respectively. Nuclei were stained with TO-PRO-3 (blue). Original magnification 40x. ( M ) Quantification of TLR2 expression by calculating the pixel ratio of positive cells in 10 different fields. TLR2 expression was significantly higher in tARPCs in basal condition and following exposure to necrotic cell supernatants. Reprinted from [Kidney International] , Supplementary Figure , Copyright (2013), with permission from Elsevier.
    Figure Legend Snippet: Difference of TLR2 expression in gARPCs and tARPCs following exposure to necrotic cell supernatants. Immunofluorescence experiments on ARPCs showing the TLR2 expression at basal conditions and after exposure to necrotic cell supernatants. ( A – D ) immunofluorescence showed the expression of TLR2 (green) in gARPCs, gARPCs + necrotic supernatant, tARPCs, and tARPCs + necrotic supernatans, respectively. ( E – H ) Immunofluorescence showed the expression of CD133 (red) in gARPCs, gARPCs + necrotic supernatant, tARPCs, and tARPCs + necrotic supernatants, respectively. ( I – L ) Double-label immunofluorescence showed the expression of TLR2 (green) and CD133 (red) in gARPCs, gARPCs + necrotic supernatant, tARPCs, and tARPCs + necrotic supernatant, respectively. Nuclei were stained with TO-PRO-3 (blue). Original magnification 40x. ( M ) Quantification of TLR2 expression by calculating the pixel ratio of positive cells in 10 different fields. TLR2 expression was significantly higher in tARPCs in basal condition and following exposure to necrotic cell supernatants. Reprinted from [Kidney International] , Supplementary Figure , Copyright (2013), with permission from Elsevier.

    Techniques Used: Expressing, Immunofluorescence, Staining

    Inhibin-A and decorin were involved in the RPTEC repair. ( A ) Preconditioned supernatants from co-cultures induced an increase of RPTEC proliferation rate. Supernatants treated with 1 U/ml Rnase did not influence the proliferation rate. ( B – C ) Representative micrographs of transmission electron microscopy showing the release of MVs from the surface of a tARPC. Micrographs show the extrusion of MVs from the surface of the tARPC. Ultrathin sections, stained with led citrate were viewed by ZEISS EM910 electron microscope. Image acquisitions were performed with magnification of ×16000. ( D ) MVs isolated from the medium of regenerative condition (cisplatin-damaged RPTECs in co-culture with tARPCs), carried high levels of Inhb-A mRNA, these levels are comparable to the ones found in the supernatant of non-damaged RPTECs. Moreover, Inhb-A levels sharply decreased when TLR2 on tARPCs was blocked. ( E ) MVs isolated from the medium of the regenerative condition (cisplatin-damaged RPTECs in co-culture with tARPCs), carried high levels of Decorin mRNA, these levels were even higher than the ones detected in the supernatant of non-damaged RPTECs. Moreover, Decorin levels sharply decreased when TLR2 on tARPCs was blocked. ( F ) Inhb-A protein level increased in the regenerative condition (cisplatin-damaged RPTECs in co-culture with tARPCs), and this increase was abrogated when TLR2 receptor was blocked reaching levels similar to those obtained in the damaged condition. Inhb-A did not increased in the co-cultures of gARPCs with damaged-RPTECs. ( G ) BrdU cell proliferation assays showed that if we treated the regenerative medium (cisplatin-damaged RPTECs in co-culture with tARPCs) with the Inhb-A blocking antibody the damaged-RPTEC failed to recover their proliferation rate. Plots represent 5 independent experiments using tARPCs from 5 different subjects; *P < 0.05, **P < 0.005.
    Figure Legend Snippet: Inhibin-A and decorin were involved in the RPTEC repair. ( A ) Preconditioned supernatants from co-cultures induced an increase of RPTEC proliferation rate. Supernatants treated with 1 U/ml Rnase did not influence the proliferation rate. ( B – C ) Representative micrographs of transmission electron microscopy showing the release of MVs from the surface of a tARPC. Micrographs show the extrusion of MVs from the surface of the tARPC. Ultrathin sections, stained with led citrate were viewed by ZEISS EM910 electron microscope. Image acquisitions were performed with magnification of ×16000. ( D ) MVs isolated from the medium of regenerative condition (cisplatin-damaged RPTECs in co-culture with tARPCs), carried high levels of Inhb-A mRNA, these levels are comparable to the ones found in the supernatant of non-damaged RPTECs. Moreover, Inhb-A levels sharply decreased when TLR2 on tARPCs was blocked. ( E ) MVs isolated from the medium of the regenerative condition (cisplatin-damaged RPTECs in co-culture with tARPCs), carried high levels of Decorin mRNA, these levels were even higher than the ones detected in the supernatant of non-damaged RPTECs. Moreover, Decorin levels sharply decreased when TLR2 on tARPCs was blocked. ( F ) Inhb-A protein level increased in the regenerative condition (cisplatin-damaged RPTECs in co-culture with tARPCs), and this increase was abrogated when TLR2 receptor was blocked reaching levels similar to those obtained in the damaged condition. Inhb-A did not increased in the co-cultures of gARPCs with damaged-RPTECs. ( G ) BrdU cell proliferation assays showed that if we treated the regenerative medium (cisplatin-damaged RPTECs in co-culture with tARPCs) with the Inhb-A blocking antibody the damaged-RPTEC failed to recover their proliferation rate. Plots represent 5 independent experiments using tARPCs from 5 different subjects; *P < 0.05, **P < 0.005.

    Techniques Used: Transmission Assay, Electron Microscopy, Staining, Microscopy, Isolation, Co-Culture Assay, Blocking Assay

    anti tlr2 mouse igg1 mab t2 5  (Hycult Biotech)


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    Hycult Biotech anti tlr2 mouse igg1 mab t2 5
    Anti Tlr2 Mouse Igg1 Mab T2 5, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tlr2 mouse igg1 mab t2 5/product/Hycult Biotech
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    mouse  (Hycult Biotech)


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    Hycult Biotech mouse
    Mouse, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse/product/Hycult Biotech
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    rat tlr2 staining mouse igg2a human anti tlr2 monoclonal  (Hycult Biotech)


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    Hycult Biotech rat tlr2 staining mouse igg2a human anti tlr2 monoclonal
    Rat Tlr2 Staining Mouse Igg2a Human Anti Tlr2 Monoclonal, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat tlr2 staining mouse igg2a human anti tlr2 monoclonal/product/Hycult Biotech
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    • human tlr2 antibody  (Hycult Biotech)
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    Hycult Biotech human tlr2 antibody
    Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with <t>TLR2</t> agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001
    Human Tlr2 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human tlr2 mab  (Hycult Biotech)
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    Hycult Biotech mouse anti human tlr2 mab
    ( A ) <t>TLR2</t> expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.
    Mouse Anti Human Tlr2 Mab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human tlr2 mab/product/Hycult Biotech
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    t2 5 monoclonal anti tlr2 ab  (Hycult Biotech)
    93
    Hycult Biotech t2 5 monoclonal anti tlr2 ab
    ( A ) <t>TLR2</t> expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.
    T2 5 Monoclonal Anti Tlr2 Ab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t2 5 monoclonal anti tlr2 ab/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
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    t2 5  (Hycult Biotech)
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    Image Search Results


    Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with TLR2 agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma

    doi: 10.1007/s12032-022-01664-5

    Figure Lengend Snippet: Cytokine production in PGK, DOK and SCC-4 cells. PGK, DOK and SCC-4 were untreated or stimulated for 24 h with TLR2 agonists: Pam3CSK4 (0.2 μg/ml), Pam2CSK4 (0.2 μg/ml) or TLR4 agonist LPS (0.5 μg/ml). Levels of ( A ) IL-6, ( B ) IL-11, ( C ) IL-8 and ( D ) TNF-α in the culture supernatants were determined by ELISA. Results were plotted using Graphpad prism 8. Data shown are representative of at least three independent experiments. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Tukey’s test to compare mean values between untreated and treated groups within the cell line, * p < 0.05; ** p < 0.01; *** p < 0.001

    Article Snippet: Antibody information: Human anti-IL-6Ra (MAB227), Mouse monoclonal IgG 1 , clone 17506 (R&D systems); Human IL-6 (MAB2061), 1936 (R&D systems); Isotype Control (MAB004), mouse monoclonal IgG 2B , clone 20116 (R&D systems); Human TLR2 antibody (HM1054), mouse monoclonal IgG 1, Clone T2.5 (Hycult Biotech).

    Techniques: Enzyme-linked Immunosorbent Assay

    The effect of TLR2 neutralising antibody on IL-6 production in SCC-4 cells. ( A ) DOK and ( B ) SCC-4 cells were untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam2CSK4 (0.2 μg/ml) and Pam3CSK4 (0.2 μg/ml) for 24 h. Culture supernatants were analysed for IL-6 secretion by ELISA. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Dunnett’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma

    doi: 10.1007/s12032-022-01664-5

    Figure Lengend Snippet: The effect of TLR2 neutralising antibody on IL-6 production in SCC-4 cells. ( A ) DOK and ( B ) SCC-4 cells were untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam2CSK4 (0.2 μg/ml) and Pam3CSK4 (0.2 μg/ml) for 24 h. Culture supernatants were analysed for IL-6 secretion by ELISA. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Dunnett’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Article Snippet: Antibody information: Human anti-IL-6Ra (MAB227), Mouse monoclonal IgG 1 , clone 17506 (R&D systems); Human IL-6 (MAB2061), 1936 (R&D systems); Isotype Control (MAB004), mouse monoclonal IgG 2B , clone 20116 (R&D systems); Human TLR2 antibody (HM1054), mouse monoclonal IgG 1, Clone T2.5 (Hycult Biotech).

    Techniques: Enzyme-linked Immunosorbent Assay

    TLR2 and TLR6 expression levels in SCC-4 cells and DOK cells. DOK and SCC-4 cells were left untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam3CSK4 (0.05 μg/ml) or Pam2CSK4 (0.05 μg/ml) for 24 h. Lysates were collected and samples were run on 12% SDS-PAGE gel. Western Blot were probed with anti-TLR2 and anti-TLR6 antibodies with anti-GAPDH used as a loading control. Experiment was done in triplicate. Densitometric analysis was performed using image lab software on TLR2, TLR6 and GAPDH blots. Sample blots for ( A ) DOK and ( B ) SCC-4 and collated densitometry data for ( C ) TLR2 and ( D ) TLR6 are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using two-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma

    doi: 10.1007/s12032-022-01664-5

    Figure Lengend Snippet: TLR2 and TLR6 expression levels in SCC-4 cells and DOK cells. DOK and SCC-4 cells were left untreated or pre-treated with anti-TLR2 Ab (10 μg/ml) for 1 h, prior to stimulation with Pam3CSK4 (0.05 μg/ml) or Pam2CSK4 (0.05 μg/ml) for 24 h. Lysates were collected and samples were run on 12% SDS-PAGE gel. Western Blot were probed with anti-TLR2 and anti-TLR6 antibodies with anti-GAPDH used as a loading control. Experiment was done in triplicate. Densitometric analysis was performed using image lab software on TLR2, TLR6 and GAPDH blots. Sample blots for ( A ) DOK and ( B ) SCC-4 and collated densitometry data for ( C ) TLR2 and ( D ) TLR6 are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using two-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, * p < 0.05; ** p < 0.01; *** p < 0.001

    Article Snippet: Antibody information: Human anti-IL-6Ra (MAB227), Mouse monoclonal IgG 1 , clone 17506 (R&D systems); Human IL-6 (MAB2061), 1936 (R&D systems); Isotype Control (MAB004), mouse monoclonal IgG 2B , clone 20116 (R&D systems); Human TLR2 antibody (HM1054), mouse monoclonal IgG 1, Clone T2.5 (Hycult Biotech).

    Techniques: Expressing, SDS Page, Western Blot, Software

    Survival rate in anoikis assays DOK and SCC-4 cells treated with rhIL-6, IL-6 neutralising antibody, IL-6Ra antibody, IL-6Ra + rhIL-6, Pam2CSK4, anti-TLR2 neutralising antibody + Pam2CSK4, and anti-TLR2 neutralising antibody + Pam2CSK4 for 24 h. Tissue culture plates were coated with 200 μl poly-(hydroxyethyl methacrylic) acid (poly-HEMA) thus inhibiting the ability of the cells to attach to the tissue culture plastic. ( A ) DOK and ( B ) SCC-4 cells were then seeded at 100,000 cells/well and allowed to attach. For those cells to be treated with 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, 40 μg/ml IL-6Ra antibody, 40 μg/ml IL-6Ra + 30 ng/ml rhIL-6, 0.2 μg/ml Pam2CSK4, and 10 μg/ml anti-TLR2 neutralising antibody + 0.2 μg/ml Pam2CSK4, complete medium was used as control, as well as medium containing 10 μg/ml isotype IgG antibody. After 24 h, 50 μl of alamarBlue ® dye was added to each well and incubated again at 37 °C/5% CO 2 for 3.5 h. SCC-4 cells demonstrated significant resistance to anoikis (% survival) when treated with 30 ng/ml rhIL-6 and 0.2 μg/ml Pam2CSK4 compared to the control group. There is a100% survival of adherent cells in the uncoated plates. Fluorescence was measured using a SpectraMax Gemini plate reader at excitation wavelength 544 nm and emission wavelength 590 nm. Results were plotted using Graphpad prism 8. Results represent at least three experiments performed in duplicate (mean ± SEM ( n )). Statistical analysis was performed one-way ANOVA with a post hoc Tukey’s test to compare mean values among the groups, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma

    doi: 10.1007/s12032-022-01664-5

    Figure Lengend Snippet: Survival rate in anoikis assays DOK and SCC-4 cells treated with rhIL-6, IL-6 neutralising antibody, IL-6Ra antibody, IL-6Ra + rhIL-6, Pam2CSK4, anti-TLR2 neutralising antibody + Pam2CSK4, and anti-TLR2 neutralising antibody + Pam2CSK4 for 24 h. Tissue culture plates were coated with 200 μl poly-(hydroxyethyl methacrylic) acid (poly-HEMA) thus inhibiting the ability of the cells to attach to the tissue culture plastic. ( A ) DOK and ( B ) SCC-4 cells were then seeded at 100,000 cells/well and allowed to attach. For those cells to be treated with 30 ng/ml rhIL-6, 10 μg/ml IL-6 neutralising antibody, 40 μg/ml IL-6Ra antibody, 40 μg/ml IL-6Ra + 30 ng/ml rhIL-6, 0.2 μg/ml Pam2CSK4, and 10 μg/ml anti-TLR2 neutralising antibody + 0.2 μg/ml Pam2CSK4, complete medium was used as control, as well as medium containing 10 μg/ml isotype IgG antibody. After 24 h, 50 μl of alamarBlue ® dye was added to each well and incubated again at 37 °C/5% CO 2 for 3.5 h. SCC-4 cells demonstrated significant resistance to anoikis (% survival) when treated with 30 ng/ml rhIL-6 and 0.2 μg/ml Pam2CSK4 compared to the control group. There is a100% survival of adherent cells in the uncoated plates. Fluorescence was measured using a SpectraMax Gemini plate reader at excitation wavelength 544 nm and emission wavelength 590 nm. Results were plotted using Graphpad prism 8. Results represent at least three experiments performed in duplicate (mean ± SEM ( n )). Statistical analysis was performed one-way ANOVA with a post hoc Tukey’s test to compare mean values among the groups, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001

    Article Snippet: Antibody information: Human anti-IL-6Ra (MAB227), Mouse monoclonal IgG 1 , clone 17506 (R&D systems); Human IL-6 (MAB2061), 1936 (R&D systems); Isotype Control (MAB004), mouse monoclonal IgG 2B , clone 20116 (R&D systems); Human TLR2 antibody (HM1054), mouse monoclonal IgG 1, Clone T2.5 (Hycult Biotech).

    Techniques: Incubation, Fluorescence

    ( A ) TLR2 expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.

    Journal: PLoS ONE

    Article Title: miR-1915 and miR-1225-5p Regulate the Expression of CD133, PAX2 and TLR2 in Adult Renal Progenitor Cells

    doi: 10.1371/journal.pone.0068296

    Figure Lengend Snippet: ( A ) TLR2 expression levels were analyzed by real-time PCR following transfection with 25 nM miR-1225-5p mimic. Increasing the amount of miR-1225-5p within ARPCs resulted in a 1.3 fold reduction of TLR2 mRNA levels 24 hours after transfection. Expression data were normalized on the housekeeping gene β-actin. Data are representative of three independent experiments (means ± SEM), *p<0.01. ( B–C ) TLR2 protein expression after transfection with 50 nM miR-1225-5p mimic. A strong reduction of TLR2 in ARPC was found after 3 days from transfection with miR-1225-5p mimic, as shown by immunofluorescence staining. To-pro-3 counterstains nuclei (blue). Original view X63.

    Article Snippet: The cells were blocked for 1 h (BSA in PBS, pH 7.4) and then incubated with a mouse anti-human TLR2 mAb (Hycult Biotechnology) and a mouse anti-human CD133 mAb (Miltenyi Biotec) overnight at 4°C.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Immunofluorescence, Staining

    C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.

    Article Snippet: Antibodies Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques:

    using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.

    Article Snippet: Antibodies Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: Generated

    C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.

    Article Snippet: Antibodies Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: Injection, In Vitro, Incubation, Translocation Assay

    (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm

    Article Snippet: Antibodies Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: Staining, Labeling, Marker

    C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)

    Article Snippet: Antibodies Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques:

    C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: C57BL/6 mice or TLR2 KO mice underwent H/R procedure and were sacrificed at 6h or 20h TP. (A) IL-6, (B) IP10 (C) KC, (D) MIG, (E) MCP-1, (F) ALT or (G) AST plasma parameters were displayed. n=6–8 per experimental group, line and * represent p<0.05, Data represent mean+/−SEM.

    Article Snippet: Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques:

    using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: using TLR2 KO and C57/Bl6 mice WT/WT, WT/KO, KO/KO and KO/WT recipient/donor chimeric mice combinations were generated. Chimeric mice underwent H/R procedure and were sacrificed at 20h TP. (A) ALT, (B) IL-6, (C) IP10, (D) MCP-1, and (E) MIG plasma parameters were displayed. n=6–8 per experimental group, * p<0.05, Data represent mean+/−SEM.

    Article Snippet: Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: Generated

    C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: C57/BL6 mice were injected i.p. with thioglycollate and peritoneal macrophages were harvested. Macrophages were stimulated in vitro with (A) PAM3CSK (1μg/ml), (B) ultrapure LPS (2ng/ml) or (C) PBS for 15min, 30min or 45min after incubation with different mAB (T2.5, 5E3, T2.5+5E3 or control, 10μg/ml). Translocation of NFκB p65 were measured and displayed in arbitrary units (n=8 or 24) per experimental group. Blue line represent mean of un-stimulated control (49+/−4 AU). Black lines represent *p<0.05 for 30min TP comparing groups, data represent mean+/−SD.

    Article Snippet: Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: Injection, In Vitro, Incubation, Translocation Assay

    (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: (A) H&E staining of liver tissue sections from monoclonal TLR2 Antibody T2.5 (labeled as “TLR2”) or corresponding isotype control mAB (labeled as “control”) pretreated animals are displayed. Mice underwent H/R or sham procedure (A1 – A4). (B) H&E staining of paraformaldehyde-fixed and paraffin-embedded lung from anti-TLR2 or isotype control mAB are displayed. Mice underwent H/R or sham procedure (B1 – B4). Plasma ALT levels of untreated control animals (white bars) or ALT plasma levels of isotype control mAB (black bars) or anti-TLR2 mAB (grey bars) treated mice after H/R procedure are displayed. Blue line represent mean of sham group plasma levels of mice receiving isotype control mAB (C). Average of lung injury using interstitial edema, alveolar edema, hemorrhage, and neutrophil infiltrates as marker (scale in arbitrary units from 0=min to 16=max) White bar displays sham mice receiving isotype control or anti TLR2 mAB, grey and black bars displays corresponding H/R group (D). N=6–16 per experimental group, *p<0.05 (C) or *p<0.01 (D), Data represent mean+/−SEM, scale bar 100μm

    Article Snippet: Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: Staining, Labeling, Marker

    C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)

    Journal: Shock (Augusta, Ga.)

    Article Title: TLR2 on bone marrow and non-bone marrow derived cells regulates inflammation and organ injury in cooperation with TLR4 during resuscitated hemorrhagic shock

    doi: 10.1097/SHK.0000000000000650

    Figure Lengend Snippet: C57/Bl6 mice were pretreated with 100μg of control mAB or T2.5 mAB and/or 5E3 mAB 30min before H/R procedure. (A) ALT plasma levels in U/l are displayed, (B–F) IL-6, MIG, MCP-1, IP-10 and KC are shown at two different time points (6h and 20h TP). significant differences between two groups are marked by a line on top of the bars. Blue lines illustrate mean plasma levels of sham control mice, dotted line illustrate uninjured control plasma levels. (n=6–14 per experimental group, *p<0.05, Data represent mean+/−SEM)

    Article Snippet: Anti-TLR2 mouse IgG1 mAb T2.5 was from HyCult Biotechnology (Uden, Neitherlands) and has been described previously ( 23 ).

    Techniques: