Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: Primers Used in qPCR

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Sequencing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: Primers Used in Chromatin Isolation by RNA Precipitation (CHIRP)

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Isolation, Sequencing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: BCAR4 regulated the downstream proteins of GLI2. Results of chromatin isolation by RNA precipitation (CHIRP) assay showed that levels of the GLI2 downstream proteins (A) RPS3, (B) IL-6, (C) MUC5AC, and (D) TGF-β1 were dramatically higher in the BCAR4 group. * p < 0.05, compared with the LacZ [negative control (NC)] group.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Isolation, Negative Control

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: BCAR4 regulated GLI2 expression. The mRNA expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group of (A) A549 and (B) HCC827 cells. * p < 0.05, ** p < 0.01, compared with the NC group. (C–F) In both cell lines, the protein expression of RPS3, IL-6, MUC5AC, and TGF-β1 was significantly lower in the si-BCAR4 group. * p < 0.05, ** p < 0.01, compared with the NC group.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Expressing

Journal: Oncology Research

Article Title: lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 ( GLI2 )

doi: 10.3727/096504018X15220594629967

Figure Lengend Snippet: GLI2 downstream protein expression was positively correlated with BCAR4 expression. (A) Results of the qRT-PCR showed that the expression levels of RPS3, IL-6, MUC5AC, and TGF-β1 were much higher in NSCLC tissues than in adjacent tissues. * p < 0.05, compared with adjacent tissues. The expression levels of (B) RPS3, (C) IL-6, (D) MUC5AC, and (E) TGF-β1 were positively correlated with BCAR4 expression.

Article Snippet: The primary antibodies were as follows: mouse anti-human RPS3, IL-6, MUC5AC, TGF-β1, and GAPDH (1:500; Cell Signaling Technology Inc., Beverly, MA, USA).

Techniques: Expressing, Quantitative RT-PCR