mouse anti human tra 1 81 monoclonal antibody  (Millipore)


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    Millipore mouse anti human tra 1 81 monoclonal antibody
    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, <t>TRA-1-81,</t> OCT-4 and NANOG.
    Mouse Anti Human Tra 1 81 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human tra 1 81 monoclonal antibody/product/Millipore
    Average 87 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    mouse anti human tra 1 81 monoclonal antibody - by Bioz Stars, 2020-08
    87/100 stars

    Images

    1) Product Images from "X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging"

    Article Title: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2014.2044

    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.
    Figure Legend Snippet: Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Techniques Used: Staining, Expressing, Binding Assay, Amplification, Real-time Polymerase Chain Reaction

    2) Product Images from "X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging"

    Article Title: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2014.2044

    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.
    Figure Legend Snippet: Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Techniques Used: Staining, Expressing, Binding Assay, Amplification, Real-time Polymerase Chain Reaction

    3) Product Images from "X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging"

    Article Title: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2014.2044

    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.
    Figure Legend Snippet: Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Techniques Used: Staining, Expressing, Binding Assay, Amplification, Real-time Polymerase Chain Reaction

    4) Product Images from "X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging"

    Article Title: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2014.2044

    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.
    Figure Legend Snippet: Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Techniques Used: Staining, Expressing, Binding Assay, Amplification, Real-time Polymerase Chain Reaction

    Related Articles

    Far Western Blot:

    Article Title: Autoantibodies to Endothelial Cell Surface ATP Synthase, the Endogenous Receptor for Hsp60, Might Play a Pathogenic Role in Vasculatides
    Article Snippet: .. Western and Far Western blotting Proteins were transferred onto PVDF membranes and probed with anti-hsp60 mAb (clone LK-1, Calbiochem) or anti-ATP synthase mAb (clone 3D5, Abcam), amplified with biotinylated anti-Ig Ab (Jackson), and developed with horseradish peroxydase (HRP)-conjugated streptavidin (Amersham). .. For the Far WB experiments, PVDF membranes were first incubated with 2 µg/ml recombinant hsp60 (Sigma), and then probed with anti-hsp60 mAb before development.

    Western Blot:

    Article Title: Autoantibodies to Endothelial Cell Surface ATP Synthase, the Endogenous Receptor for Hsp60, Might Play a Pathogenic Role in Vasculatides
    Article Snippet: .. Western and Far Western blotting Proteins were transferred onto PVDF membranes and probed with anti-hsp60 mAb (clone LK-1, Calbiochem) or anti-ATP synthase mAb (clone 3D5, Abcam), amplified with biotinylated anti-Ig Ab (Jackson), and developed with horseradish peroxydase (HRP)-conjugated streptavidin (Amersham). .. For the Far WB experiments, PVDF membranes were first incubated with 2 µg/ml recombinant hsp60 (Sigma), and then probed with anti-hsp60 mAb before development.

    Article Title: Unusual Kinetic and Structural Properties Control Rapid Assembly and Turnover of Actin in the Parasite Toxoplasma gondii D⃞
    Article Snippet: .. In some experiments, fractions were detected by Western blotting using rabbit anti-TgACT1 antiserum ( ) or mAb C4 (Chemicon International, Temecula, CA), followed by peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories), and chemiluminescence using ECL Plus (Amersham Biosciences). ..

    Incubation:

    Article Title: Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis
    Article Snippet: .. We added human mAbs, 5F5, 2G6, 1D1, and control isotype 6A at 5 μg/mL (100 μl/well), or 5 μg/mL anti-NR1 mAb (MAB 1586 R1JHL, Millipore), in triplicate samples, and incubated for 1 h at 37 °C. .. After three additional PBST washes, secondary antibodies were added, either an anti-human IgG HRP conjugate (9040–05 SouthernBiotech, Birmingham, AL) or anti-mouse IgG HRP conjugate (1010–05, Southern Biotech), at 1:2000 and incubated for 1 h at 37 °C, followed by 3 washes.

    Amplification:

    Article Title: Autoantibodies to Endothelial Cell Surface ATP Synthase, the Endogenous Receptor for Hsp60, Might Play a Pathogenic Role in Vasculatides
    Article Snippet: .. Western and Far Western blotting Proteins were transferred onto PVDF membranes and probed with anti-hsp60 mAb (clone LK-1, Calbiochem) or anti-ATP synthase mAb (clone 3D5, Abcam), amplified with biotinylated anti-Ig Ab (Jackson), and developed with horseradish peroxydase (HRP)-conjugated streptavidin (Amersham). .. For the Far WB experiments, PVDF membranes were first incubated with 2 µg/ml recombinant hsp60 (Sigma), and then probed with anti-hsp60 mAb before development.

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  • 87
    Millipore mouse anti human nanog homeobox nanog monoclonal antibody monoclonal antibody cat no mabd24a4
    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), <t>Nanog</t> <t>homeobox</t> (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.
    Mouse Anti Human Nanog Homeobox Nanog Monoclonal Antibody Monoclonal Antibody Cat No Mabd24a4, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human nanog homeobox nanog monoclonal antibody monoclonal antibody cat no mabd24a4/product/Millipore
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    mouse anti human nanog homeobox nanog monoclonal antibody monoclonal antibody cat no mabd24a4 - by Bioz Stars, 2020-08
    87/100 stars
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    Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Journal: International Journal of Molecular Medicine

    Article Title: X chromosome inactivation in human parthenogenetic embryonic stem cells following prolonged passaging

    doi: 10.3892/ijmm.2014.2044

    Figure Lengend Snippet: Pluripotentiality of human parthenogenetic embryonic stem cell line-2 (hPES-2). (A) Alkaline phosphatase (AP) staining, (B) embryoid bodies (EBs), (C) pluripotency gene expression, including octamer-binding transcription factor 4 (OCT-4), REX1 [also referred to as zinc-finger protein-42 (Zfp42)], SRY (sex determining region Y)-box 2 (SOX2), Nanog homeobox (NANOG), Lin-28 homolog A (LIN28) and nucleophosmin (β-actin was used as a control), amplified by real-time PCR, and (D) pluripotency immunofluorescent markers, including stage-specific embryonic antigen (SSEA)3, SSEA4, tumor-rejection antigen (TRA)-1-60, TRA-1-81, OCT-4 and NANOG.

    Article Snippet: Briefly, for immunofluorescence staining, the hPES-2 cell colonies were incubated with the following primary antibodies against stage-specific embryonic antigens: rat anti-human stage-specific embryonic antigen (SSEA)3 monoclonal antibody (Cat. no. LV1528429), rat anti-human SSEA4 monoclonal antibody (Cat. no. LV1488380), mouse anti-human tumor-rejection antigen (TRA)-1-60 monoclonal antibody (Cat. no. LV1541028), mouse anti-human TRA-1-81 monoclonal antibody (Cat. no. LV1580855); mouse anti-human octamer-binding transcription factor 4 (OCT-4) monoclonal antibody (Cat. no. MAB4419A4) and mouse anti-human Nanog homeobox (NANOG) monoclonal antibody (Cat. no. MABD24A4) (all from Millipore) (all were used at 1:100).

    Techniques: Staining, Expressing, Binding Assay, Amplification, Real-time Polymerase Chain Reaction