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Bio-Rad human antibodies
Human Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human antibodies/product/Bio-Rad
Average 93 stars, based on 1 article reviews
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human antibodies - by Bioz Stars, 2023-11
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Bio-Rad mca509g
List of lectin and antibodies.
Mca509g, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mca509g/product/Bio-Rad
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mca509g - by Bioz Stars, 2023-11
93/100 stars

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1) Product Images from "Mechanisms of FH Protection Against Neovascular AMD"

Article Title: Mechanisms of FH Protection Against Neovascular AMD

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2020.00443

List of lectin and antibodies.
Figure Legend Snippet: List of lectin and antibodies.

Techniques Used: Plasmid Preparation


Structured Review

Bio-Rad ox 24 mouse
List of lectin and antibodies.
Ox 24 Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ox 24 mouse/product/Bio-Rad
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ox 24 mouse - by Bioz Stars, 2023-11
93/100 stars

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1) Product Images from "Mechanisms of FH Protection Against Neovascular AMD"

Article Title: Mechanisms of FH Protection Against Neovascular AMD

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2020.00443

List of lectin and antibodies.
Figure Legend Snippet: List of lectin and antibodies.

Techniques Used: Plasmid Preparation


Structured Review

Bio-Rad anti h
Anti H, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti h/product/Bio-Rad
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti h - by Bioz Stars, 2023-11
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Bio-Rad mouse anti cfh monoclonal antibody
Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) <t>Cfh</t> mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L <t>);</t> <t>ELISA</t> control standard curves and protein concentrations in the basal supernatants are shown in the .
Mouse Anti Cfh Monoclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti cfh monoclonal antibody/product/Bio-Rad
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti cfh monoclonal antibody - by Bioz Stars, 2023-11
93/100 stars

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1) Product Images from "Oxidative Stress Increases Endogenous Complement-Dependent Inflammatory and Angiogenic Responses in Retinal Pigment Epithelial Cells Independently of Exogenous Complement Sources"

Article Title: Oxidative Stress Increases Endogenous Complement-Dependent Inflammatory and Angiogenic Responses in Retinal Pigment Epithelial Cells Independently of Exogenous Complement Sources

Journal: Antioxidants

doi: 10.3390/antiox8110548

Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); ELISA control standard curves and protein concentrations in the basal supernatants are shown in the .
Figure Legend Snippet: Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); ELISA control standard curves and protein concentrations in the basal supernatants are shown in the .

Techniques Used: Immunohistochemistry, Protein Concentration, Standard Deviation, Enzyme-linked Immunosorbent Assay


Structured Review

Bio-Rad 92r anti human ccr9 mab
<t>92R</t> mAb identifies human chemokine receptor <t>CCR9.</t> (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.
92r Anti Human Ccr9 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/92r anti human ccr9 mab/product/Bio-Rad
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
92r anti human ccr9 mab - by Bioz Stars, 2023-11
93/100 stars

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1) Product Images from "92R Monoclonal Antibody Inhibits Human CCR9 + Leukemia Cells Growth in NSG Mice Xenografts"

Article Title: 92R Monoclonal Antibody Inhibits Human CCR9 + Leukemia Cells Growth in NSG Mice Xenografts

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2018.00077

92R mAb identifies human chemokine receptor CCR9. (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.
Figure Legend Snippet: 92R mAb identifies human chemokine receptor CCR9. (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.

Techniques Used: Flow Cytometry, Staining, Stable Transfection, Transfection, Plasmid Preparation

92R and 91R mAbs compete with each other for binding to hCCR9. (A) Competitive binding analyses to CCR9 + MOLT-4 cells. Cells were preincubated with unlabeled 3C3, 91R, 92R anti-CCR9 mAbs, or their isotype controls (IgG2a or IgG2b) and, without washing the antibody excess, stained with either biotinylated 91R (top row) or biotinylated 92R (bottom row). After washing, binding of the biotinylated antibodies to the MOLT-4 cells was revealed with streptavidin-FITC and analyzed by flow cytometry. (B) Enzyme-linked immunosorbent assay competitive binding analysis of anti-CCR9 mAbs to the hCCR9 (aa 2–22) peptide using biotin-labeled 91R and 92R in the presence of unlabeled competitors (IgG2a-isotype control, IgG2b-isotype control, 91R, or 92R) and revealed with peroxidase conjugated streptavidin. *** P < 0.001.
Figure Legend Snippet: 92R and 91R mAbs compete with each other for binding to hCCR9. (A) Competitive binding analyses to CCR9 + MOLT-4 cells. Cells were preincubated with unlabeled 3C3, 91R, 92R anti-CCR9 mAbs, or their isotype controls (IgG2a or IgG2b) and, without washing the antibody excess, stained with either biotinylated 91R (top row) or biotinylated 92R (bottom row). After washing, binding of the biotinylated antibodies to the MOLT-4 cells was revealed with streptavidin-FITC and analyzed by flow cytometry. (B) Enzyme-linked immunosorbent assay competitive binding analysis of anti-CCR9 mAbs to the hCCR9 (aa 2–22) peptide using biotin-labeled 91R and 92R in the presence of unlabeled competitors (IgG2a-isotype control, IgG2b-isotype control, 91R, or 92R) and revealed with peroxidase conjugated streptavidin. *** P < 0.001.

Techniques Used: Binding Assay, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Labeling

92R and 91R mAbs failed to inhibit CCL25-mediated migration of the CCR9 + MOLT-4 cells. (A) Migration response of MOLT-4 cells to different CCL25 concentrations. (B) 200 nM CCL25, in the absence of antibody was used to determine the highest number of migrating cells (defined as 100% migration) and to compare with similar experiments where 91R, 92R, or their isotype control antibodies (IgG2g or IgG2a, respectively) were added. In addition, the anti-CCR9 mAb 3C3, known to inhibit the CCL25:CCR9 interaction was used as positive control for migration inhibition.
Figure Legend Snippet: 92R and 91R mAbs failed to inhibit CCL25-mediated migration of the CCR9 + MOLT-4 cells. (A) Migration response of MOLT-4 cells to different CCL25 concentrations. (B) 200 nM CCL25, in the absence of antibody was used to determine the highest number of migrating cells (defined as 100% migration) and to compare with similar experiments where 91R, 92R, or their isotype control antibodies (IgG2g or IgG2a, respectively) were added. In addition, the anti-CCR9 mAb 3C3, known to inhibit the CCL25:CCR9 interaction was used as positive control for migration inhibition.

Techniques Used: Migration, Positive Control, Inhibition


Structured Review

Bio-Rad human recombinant klk6
Human Recombinant Klk6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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human recombinant klk6 - by Bioz Stars, 2023-11
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    Bio-Rad human antibodies
    Human Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad mca509g
    List of lectin and antibodies.
    Mca509g, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad ox 24 mouse
    List of lectin and antibodies.
    Ox 24 Mouse, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad anti h
    List of lectin and antibodies.
    Anti H, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad mouse anti cfh monoclonal antibody
    Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) <t>Cfh</t> mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L <t>);</t> <t>ELISA</t> control standard curves and protein concentrations in the basal supernatants are shown in the .
    Mouse Anti Cfh Monoclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti cfh monoclonal antibody/product/Bio-Rad
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    Bio-Rad 92r anti human ccr9 mab
    <t>92R</t> mAb identifies human chemokine receptor <t>CCR9.</t> (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.
    92r Anti Human Ccr9 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad human recombinant klk6
    <t>92R</t> mAb identifies human chemokine receptor <t>CCR9.</t> (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.
    Human Recombinant Klk6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    List of lectin and antibodies.

    Journal: Frontiers in Immunology

    Article Title: Mechanisms of FH Protection Against Neovascular AMD

    doi: 10.3389/fimmu.2020.00443

    Figure Lengend Snippet: List of lectin and antibodies.

    Article Snippet: Factor H , OX-24 (Mouse) , BIO-RAD , France , MCA509G.

    Techniques: Plasmid Preparation

    List of lectin and antibodies.

    Journal: Frontiers in Immunology

    Article Title: Mechanisms of FH Protection Against Neovascular AMD

    doi: 10.3389/fimmu.2020.00443

    Figure Lengend Snippet: List of lectin and antibodies.

    Article Snippet: Factor H , OX-24 (Mouse) , BIO-RAD , France , MCA509G.

    Techniques: Plasmid Preparation

    Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); ELISA control standard curves and protein concentrations in the basal supernatants are shown in the .

    Journal: Antioxidants

    Article Title: Oxidative Stress Increases Endogenous Complement-Dependent Inflammatory and Angiogenic Responses in Retinal Pigment Epithelial Cells Independently of Exogenous Complement Sources

    doi: 10.3390/antiox8110548

    Figure Lengend Snippet: Oxidative stress induced complement component accumulation in ARPE-19 cells. ( A ) Properdin mRNA levels were increased 24 h following H 2 O 2 treatment. This did not affect ( B ) apical properdin secretion, but was confirmed in the protein level by immunohistochemistry using an ( C – E ) anti-properdin (red) antibody. ( F ) C3 mRNA and ( G ) apical C3 protein secretion were not altered in stressed ARPE-19 cells. Immunohistochemistry using ( H – J ) anti-C3 (green) antibodies showed an increase of cell-associated ( I , J ) C3 after oxidative stress treatment. ( K ) Cfh mRNA and ( L ) CFH apical protein concentration were decreased following H 2 O 2 treatment. ( M – O ) Immunohistochemistry using anti-complement factor H (CFH, purple) antibodies showed an increase in cell-associated ( N , O ) CFH after oxidative stress treatment. Mean with standard deviation is shown, ** p ≤ 0.01, **** p ≤ 0.0001; dotted line depicts untreated control ( A , F , K ); w/o untreated control ( G , G , L ); ELISA control standard curves and protein concentrations in the basal supernatants are shown in the .

    Article Snippet: CFH was quantified in an in-house ELISA with mouse anti-CFH monoclonal antibody (BioRad, Feldkirchen, Germany) as a capture antibody and goat anti-CFH polyclonal antibody (Merck, Darmstadt, Germany) as a detection antibody ( ).

    Techniques: Immunohistochemistry, Protein Concentration, Standard Deviation, Enzyme-linked Immunosorbent Assay

    92R mAb identifies human chemokine receptor CCR9. (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.

    Journal: Frontiers in Immunology

    Article Title: 92R Monoclonal Antibody Inhibits Human CCR9 + Leukemia Cells Growth in NSG Mice Xenografts

    doi: 10.3389/fimmu.2018.00077

    Figure Lengend Snippet: 92R mAb identifies human chemokine receptor CCR9. (A) Representative flow cytometry staining of HEK-293 cells stably transfected with either pCIneo-hCCR9 or the empty pCIneo vector using the mAbs 91R, 92R (empty histograms), or isotype-matched control mAbs (IgG2b and IgG2a, respectively) (filled histograms). (B) MOLT-4 and Jurkat human leukemia cells were stained with the anti-human CCR9 mAb 91R and 92R (empty histograms) or isotype-matched control mAbs (filled histograms) and analyzed by flow cytometry.

    Article Snippet: Murine 91R and 92R anti-human CCR9 mAb were raised after immunization of BALB/c mice with a gene gun (Bio-Rad) particle-mediated DNA administration of the pCIneo plasmid bearing the human CCR9 cDNA, as previously described ( ).

    Techniques: Flow Cytometry, Staining, Stable Transfection, Transfection, Plasmid Preparation

    92R and 91R mAbs compete with each other for binding to hCCR9. (A) Competitive binding analyses to CCR9 + MOLT-4 cells. Cells were preincubated with unlabeled 3C3, 91R, 92R anti-CCR9 mAbs, or their isotype controls (IgG2a or IgG2b) and, without washing the antibody excess, stained with either biotinylated 91R (top row) or biotinylated 92R (bottom row). After washing, binding of the biotinylated antibodies to the MOLT-4 cells was revealed with streptavidin-FITC and analyzed by flow cytometry. (B) Enzyme-linked immunosorbent assay competitive binding analysis of anti-CCR9 mAbs to the hCCR9 (aa 2–22) peptide using biotin-labeled 91R and 92R in the presence of unlabeled competitors (IgG2a-isotype control, IgG2b-isotype control, 91R, or 92R) and revealed with peroxidase conjugated streptavidin. *** P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: 92R Monoclonal Antibody Inhibits Human CCR9 + Leukemia Cells Growth in NSG Mice Xenografts

    doi: 10.3389/fimmu.2018.00077

    Figure Lengend Snippet: 92R and 91R mAbs compete with each other for binding to hCCR9. (A) Competitive binding analyses to CCR9 + MOLT-4 cells. Cells were preincubated with unlabeled 3C3, 91R, 92R anti-CCR9 mAbs, or their isotype controls (IgG2a or IgG2b) and, without washing the antibody excess, stained with either biotinylated 91R (top row) or biotinylated 92R (bottom row). After washing, binding of the biotinylated antibodies to the MOLT-4 cells was revealed with streptavidin-FITC and analyzed by flow cytometry. (B) Enzyme-linked immunosorbent assay competitive binding analysis of anti-CCR9 mAbs to the hCCR9 (aa 2–22) peptide using biotin-labeled 91R and 92R in the presence of unlabeled competitors (IgG2a-isotype control, IgG2b-isotype control, 91R, or 92R) and revealed with peroxidase conjugated streptavidin. *** P < 0.001.

    Article Snippet: Murine 91R and 92R anti-human CCR9 mAb were raised after immunization of BALB/c mice with a gene gun (Bio-Rad) particle-mediated DNA administration of the pCIneo plasmid bearing the human CCR9 cDNA, as previously described ( ).

    Techniques: Binding Assay, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Labeling

    92R and 91R mAbs failed to inhibit CCL25-mediated migration of the CCR9 + MOLT-4 cells. (A) Migration response of MOLT-4 cells to different CCL25 concentrations. (B) 200 nM CCL25, in the absence of antibody was used to determine the highest number of migrating cells (defined as 100% migration) and to compare with similar experiments where 91R, 92R, or their isotype control antibodies (IgG2g or IgG2a, respectively) were added. In addition, the anti-CCR9 mAb 3C3, known to inhibit the CCL25:CCR9 interaction was used as positive control for migration inhibition.

    Journal: Frontiers in Immunology

    Article Title: 92R Monoclonal Antibody Inhibits Human CCR9 + Leukemia Cells Growth in NSG Mice Xenografts

    doi: 10.3389/fimmu.2018.00077

    Figure Lengend Snippet: 92R and 91R mAbs failed to inhibit CCL25-mediated migration of the CCR9 + MOLT-4 cells. (A) Migration response of MOLT-4 cells to different CCL25 concentrations. (B) 200 nM CCL25, in the absence of antibody was used to determine the highest number of migrating cells (defined as 100% migration) and to compare with similar experiments where 91R, 92R, or their isotype control antibodies (IgG2g or IgG2a, respectively) were added. In addition, the anti-CCR9 mAb 3C3, known to inhibit the CCL25:CCR9 interaction was used as positive control for migration inhibition.

    Article Snippet: Murine 91R and 92R anti-human CCR9 mAb were raised after immunization of BALB/c mice with a gene gun (Bio-Rad) particle-mediated DNA administration of the pCIneo plasmid bearing the human CCR9 cDNA, as previously described ( ).

    Techniques: Migration, Positive Control, Inhibition