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Agilent technologies mouse anti human egfr monoclonal antibody
<t>EGFR</t> (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by <t>immunohistochemistry.</t> (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).
Mouse Anti Human Egfr Monoclonal Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti human egfr monoclonal antibody/product/Agilent technologies
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Price from $9.99 to $1999.99
mouse anti human egfr monoclonal antibody - by Bioz Stars, 2021-06
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1) Product Images from "Adrenal cortical neoplasms: a study of clinicopathological features related to epidermal growth factor receptor gene status"

Article Title: Adrenal cortical neoplasms: a study of clinicopathological features related to epidermal growth factor receptor gene status

Journal: Diagnostic Pathology

doi: 10.1186/1746-1596-9-19

EGFR (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by immunohistochemistry. (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).
Figure Legend Snippet: EGFR (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by immunohistochemistry. (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).

Techniques Used: Expressing, Immunohistochemistry, Fluorescence In Situ Hybridization, In Situ Hybridization

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Expressing:

Article Title: CAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells
Article Snippet: Then cells were washed again and stained with allophycocyanin (APC)-conjugated streptavidin (Jackson ImmunoResearch, West Grove, PA). .. To determine wtEGFR and/or EGFRvIII expression on the surface of GB cells, the cells were incubated with mouse monoclonal anti-human EGFR (clone H11, DAKO, Carpinteria, CA) which recognizes both wild-type EGFR and its mutant form (EGFRvIII), followed by staining with APC conjugated goat anti-mouse IgG second antibody. .. Reverse transcription PCR To detect EGFR mRNA expression in glioma cells, RNA was extracted from the cell lines with RNeasy Mini Kit (Qiagen, Hilden, Germany) and quantified with NanoDrop (Thermo Fisher, Wilmington, DE).

Article Title: Adrenal cortical neoplasms: a study of clinicopathological features related to epidermal growth factor receptor gene status
Article Snippet: .. Immunohistochemical study EGFR protein expression was evaluated by immunohistochemistry using a mouse anti-human EGFR monoclonal antibody (clone 2-18C9, Pharm Dx kit, Dako North America, Inc., Via Real, Carpinteria, CA, USA), according to the manufacturer’s instructions. ..

Incubation:

Article Title: CAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells
Article Snippet: Then cells were washed again and stained with allophycocyanin (APC)-conjugated streptavidin (Jackson ImmunoResearch, West Grove, PA). .. To determine wtEGFR and/or EGFRvIII expression on the surface of GB cells, the cells were incubated with mouse monoclonal anti-human EGFR (clone H11, DAKO, Carpinteria, CA) which recognizes both wild-type EGFR and its mutant form (EGFRvIII), followed by staining with APC conjugated goat anti-mouse IgG second antibody. .. Reverse transcription PCR To detect EGFR mRNA expression in glioma cells, RNA was extracted from the cell lines with RNeasy Mini Kit (Qiagen, Hilden, Germany) and quantified with NanoDrop (Thermo Fisher, Wilmington, DE).

Mutagenesis:

Article Title: CAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells
Article Snippet: Then cells were washed again and stained with allophycocyanin (APC)-conjugated streptavidin (Jackson ImmunoResearch, West Grove, PA). .. To determine wtEGFR and/or EGFRvIII expression on the surface of GB cells, the cells were incubated with mouse monoclonal anti-human EGFR (clone H11, DAKO, Carpinteria, CA) which recognizes both wild-type EGFR and its mutant form (EGFRvIII), followed by staining with APC conjugated goat anti-mouse IgG second antibody. .. Reverse transcription PCR To detect EGFR mRNA expression in glioma cells, RNA was extracted from the cell lines with RNeasy Mini Kit (Qiagen, Hilden, Germany) and quantified with NanoDrop (Thermo Fisher, Wilmington, DE).

Staining:

Article Title: CAR-Engineered NK Cells Targeting Wild-Type EGFR and EGFRvIII Enhance Killing of Glioblastoma and Patient-Derived Glioblastoma Stem Cells
Article Snippet: Then cells were washed again and stained with allophycocyanin (APC)-conjugated streptavidin (Jackson ImmunoResearch, West Grove, PA). .. To determine wtEGFR and/or EGFRvIII expression on the surface of GB cells, the cells were incubated with mouse monoclonal anti-human EGFR (clone H11, DAKO, Carpinteria, CA) which recognizes both wild-type EGFR and its mutant form (EGFRvIII), followed by staining with APC conjugated goat anti-mouse IgG second antibody. .. Reverse transcription PCR To detect EGFR mRNA expression in glioma cells, RNA was extracted from the cell lines with RNeasy Mini Kit (Qiagen, Hilden, Germany) and quantified with NanoDrop (Thermo Fisher, Wilmington, DE).

Immunohistochemistry:

Article Title: Acquired/intratumoral mutation of KRAS during metastatic progression of colorectal carcinogenesis
Article Snippet: Almost all of the procedures were performed using a BenchMark XT IHC/ISH Staining Module (Roche Diagnostics K.K; Tokyo, Japan). .. Deparaffinized 4-μm specimens were used for IHC along with anti-human EGFR mouse monoclonal antibody (clone 2-18C9, Dako Japan; Tokyo, Japan), anti-human KRAS mouse monoclonal antibody (clone ab55391, Abcam Japan; Tokyo, Japan), and polyclonal rabbit anti-human GSTP (311-H, Medical and Biological Laboratories Co., Ltd.; Nagoya, Japan). ..

Article Title: Adrenal cortical neoplasms: a study of clinicopathological features related to epidermal growth factor receptor gene status
Article Snippet: .. Immunohistochemical study EGFR protein expression was evaluated by immunohistochemistry using a mouse anti-human EGFR monoclonal antibody (clone 2-18C9, Pharm Dx kit, Dako North America, Inc., Via Real, Carpinteria, CA, USA), according to the manufacturer’s instructions. ..

Immunofluorescence:

Article Title: Localization and Molecular Characterization of human Breast Cancer Initiating Cells from heterogeneous population of Breast Cancer Mesenchymal Stem cells by Immunofluorescence Microscopy
Article Snippet: Immunofluorescence Microscopy of hBCMSCs cell line Freshly prepared coverslip cultures were washed twice with ice-cold PBS and then fixed with 4% paraformaldehyde for 20 minutes at 4°C. .. Immunofluorescence for various cancer genes such as p53 (Mouse Anti Human p53 protein, DAKO Cytomation, Denmark), RB1 (Anti Human Retinoblastoma gene, DAKO A/S, Denmark), Hras (Hras Monoclonal IgG, Santacruz Biotechnology), EGFR (Mouse Anti Human EGFR H11, DAKO Corporation, CA, USA), Ki67 (Purified AntiHumanKi67, BD Pharmingen™, SanDiego CA), GST (Mouse Anti Human glutathione-s-transferase pi DAKO Corporation, CA,USA), ETS1 (ETS1 Rabbit Polyclonal IgG, Santacruz Biotechnology), ATF2 (ATF2 Rabbit Polyclonal IgG, Santacruz Biotechnology), have been carried out using respective specific antibodies present in cancer cells. ..

Purification:

Article Title: Localization and Molecular Characterization of human Breast Cancer Initiating Cells from heterogeneous population of Breast Cancer Mesenchymal Stem cells by Immunofluorescence Microscopy
Article Snippet: Immunofluorescence Microscopy of hBCMSCs cell line Freshly prepared coverslip cultures were washed twice with ice-cold PBS and then fixed with 4% paraformaldehyde for 20 minutes at 4°C. .. Immunofluorescence for various cancer genes such as p53 (Mouse Anti Human p53 protein, DAKO Cytomation, Denmark), RB1 (Anti Human Retinoblastoma gene, DAKO A/S, Denmark), Hras (Hras Monoclonal IgG, Santacruz Biotechnology), EGFR (Mouse Anti Human EGFR H11, DAKO Corporation, CA, USA), Ki67 (Purified AntiHumanKi67, BD Pharmingen™, SanDiego CA), GST (Mouse Anti Human glutathione-s-transferase pi DAKO Corporation, CA,USA), ETS1 (ETS1 Rabbit Polyclonal IgG, Santacruz Biotechnology), ATF2 (ATF2 Rabbit Polyclonal IgG, Santacruz Biotechnology), have been carried out using respective specific antibodies present in cancer cells. ..

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  • 86
    Agilent technologies mouse anti human egfr monoclonal antibody
    <t>EGFR</t> (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by <t>immunohistochemistry.</t> (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).
    Mouse Anti Human Egfr Monoclonal Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human egfr monoclonal antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human egfr monoclonal antibody - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    97
    Agilent technologies egfr pharmdx kit
    Immunohistochemical <t>EGFR</t> staining with four different antibodies showing differences in levels of EGFR expression in the same specimen of a squamous cell carcinoma (SSC) (original magnification × 400). (A) Staining intensity with Dako <t>PharmDx</t> 2+, (B) Staining intensity with 31G7 2+, (C) Staining intensity with 2.1E1 3+, (D) Staining intensity with SP84 1+.
    Egfr Pharmdx Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr pharmdx kit/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egfr pharmdx kit - by Bioz Stars, 2021-06
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    86
    Agilent technologies anti human egfr mouse monoclonal antibody
    Immunohistochemical analyses of <t>EGFR,</t> KRAS and GSTP. (A) Negative immunoreactivity for EGFR at the primary lesion and (B) positive immunoreactivity at the site of lung metastasis in case 7, (C) negative immunoreactivity for KRAS (G12D) at the primary
    Anti Human Egfr Mouse Monoclonal Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human egfr mouse monoclonal antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human egfr mouse monoclonal antibody - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    99
    Agilent technologies antibodies against egfr
    Unidirectional phosphorylation of <t>EGFRvIII</t> by <t>EGFR</t> correlates with proliferation and transformation. (A) Chemical genetic approach: as-allele-selective irreversible inhibitor 4TB did not affect EGFR or EGFRvIII, while as-alleles of these kinases (EGFR
    Antibodies Against Egfr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against egfr/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against egfr - by Bioz Stars, 2021-06
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    Image Search Results


    EGFR (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by immunohistochemistry. (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).

    Journal: Diagnostic Pathology

    Article Title: Adrenal cortical neoplasms: a study of clinicopathological features related to epidermal growth factor receptor gene status

    doi: 10.1186/1746-1596-9-19

    Figure Lengend Snippet: EGFR (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by immunohistochemistry. (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).

    Article Snippet: Immunohistochemical study EGFR protein expression was evaluated by immunohistochemistry using a mouse anti-human EGFR monoclonal antibody (clone 2-18C9, Pharm Dx kit, Dako North America, Inc., Via Real, Carpinteria, CA, USA), according to the manufacturer’s instructions.

    Techniques: Expressing, Immunohistochemistry, Fluorescence In Situ Hybridization, In Situ Hybridization

    Immunohistochemical EGFR staining with four different antibodies showing differences in levels of EGFR expression in the same specimen of a squamous cell carcinoma (SSC) (original magnification × 400). (A) Staining intensity with Dako PharmDx 2+, (B) Staining intensity with 31G7 2+, (C) Staining intensity with 2.1E1 3+, (D) Staining intensity with SP84 1+.

    Journal: Diagnostic Pathology

    Article Title: Correlation of EGFR expression, gene copy number and clinicopathological status in NSCLC

    doi: 10.1186/s13000-014-0165-0

    Figure Lengend Snippet: Immunohistochemical EGFR staining with four different antibodies showing differences in levels of EGFR expression in the same specimen of a squamous cell carcinoma (SSC) (original magnification × 400). (A) Staining intensity with Dako PharmDx 2+, (B) Staining intensity with 31G7 2+, (C) Staining intensity with 2.1E1 3+, (D) Staining intensity with SP84 1+.

    Article Snippet: Immunohistochemistry EGFR protein expression was assessed by immunohistochemistry on 2 μm deparaffinized TMA sections, using four EGFR specific antibodies: the Food and Drug Administration (FDA) approved Dako EGFR PharmDx kit (clone 2-18C9, mouse monoclonal, prediluted, DAKO, Corp., Glostrup, Denmark), Zymed antibody (clone 31G7, mouse monoclonal, 1:30, Zymed laboratories, San Francisco, CA), Zytomed antibody (clone 2.1E1, mouse monoclonal, 1:100, Zytomed Systems, Berlin, Germany) and antibody clone SP84 (rabbit monoclonal, 1:100, Spring Bioscience, CA).

    Techniques: Immunohistochemistry, Staining, Expressing

    Immunohistochemical analyses of EGFR, KRAS and GSTP. (A) Negative immunoreactivity for EGFR at the primary lesion and (B) positive immunoreactivity at the site of lung metastasis in case 7, (C) negative immunoreactivity for KRAS (G12D) at the primary

    Journal: Oncology Letters

    Article Title: Acquired/intratumoral mutation of KRAS during metastatic progression of colorectal carcinogenesis

    doi: 10.3892/ol.2011.543

    Figure Lengend Snippet: Immunohistochemical analyses of EGFR, KRAS and GSTP. (A) Negative immunoreactivity for EGFR at the primary lesion and (B) positive immunoreactivity at the site of lung metastasis in case 7, (C) negative immunoreactivity for KRAS (G12D) at the primary

    Article Snippet: Deparaffinized 4-μm specimens were used for IHC along with anti-human EGFR mouse monoclonal antibody (clone 2-18C9, Dako Japan; Tokyo, Japan), anti-human KRAS mouse monoclonal antibody (clone ab55391, Abcam Japan; Tokyo, Japan), and polyclonal rabbit anti-human GSTP (311-H, Medical and Biological Laboratories Co., Ltd.; Nagoya, Japan).

    Techniques: Immunohistochemistry

    Unidirectional phosphorylation of EGFRvIII by EGFR correlates with proliferation and transformation. (A) Chemical genetic approach: as-allele-selective irreversible inhibitor 4TB did not affect EGFR or EGFRvIII, while as-alleles of these kinases (EGFR

    Journal: Cancer cell

    Article Title: EGFR phosphorylates tumor-derived EGFRvIII driving STAT3/5 and progression in glioblastoma

    doi: 10.1016/j.ccr.2013.09.004

    Figure Lengend Snippet: Unidirectional phosphorylation of EGFRvIII by EGFR correlates with proliferation and transformation. (A) Chemical genetic approach: as-allele-selective irreversible inhibitor 4TB did not affect EGFR or EGFRvIII, while as-alleles of these kinases (EGFR

    Article Snippet: Sections were immunostained with antibodies against EGFR (mouse monoclonal DAK-H1-WT, Dako, diluted 1:200), EGFRvIII (rabbit polyclonal 6549, Celldex, diluted 1:5000), p-STAT3 (Tyr705) (rabbit monoclonal antibody D3A7 or mouse monoclonal antibody 3E2, Cell Signaling, each diluted 1:50).

    Techniques: Transformation Assay

    Co-expression of EGFR and EGFRvIII enhances malignancy. (A, B) Anchorage-independent growth of LN-229:parent, LN-229:EGFR, LN-229:EGFRvIII, or LN-229:EGFR/EGFRvIII cells was measured by colony formation in soft agar, in the presence or absence of EGF

    Journal: Cancer cell

    Article Title: EGFR phosphorylates tumor-derived EGFRvIII driving STAT3/5 and progression in glioblastoma

    doi: 10.1016/j.ccr.2013.09.004

    Figure Lengend Snippet: Co-expression of EGFR and EGFRvIII enhances malignancy. (A, B) Anchorage-independent growth of LN-229:parent, LN-229:EGFR, LN-229:EGFRvIII, or LN-229:EGFR/EGFRvIII cells was measured by colony formation in soft agar, in the presence or absence of EGF

    Article Snippet: Sections were immunostained with antibodies against EGFR (mouse monoclonal DAK-H1-WT, Dako, diluted 1:200), EGFRvIII (rabbit polyclonal 6549, Celldex, diluted 1:5000), p-STAT3 (Tyr705) (rabbit monoclonal antibody D3A7 or mouse monoclonal antibody 3E2, Cell Signaling, each diluted 1:50).

    Techniques: Expressing

    Co-expression of EGFR and EGFRvIII is associated with phosphorylation of STAT proteins. (A) LN-229:parent, LN-229:EGFR, LN-229:EGFRvIII, or LN-229:EGFR/EGFRvIII cells were serum-staved for 24 hr then treated with or without EGF (50 ng/ml) for 15 min prior

    Journal: Cancer cell

    Article Title: EGFR phosphorylates tumor-derived EGFRvIII driving STAT3/5 and progression in glioblastoma

    doi: 10.1016/j.ccr.2013.09.004

    Figure Lengend Snippet: Co-expression of EGFR and EGFRvIII is associated with phosphorylation of STAT proteins. (A) LN-229:parent, LN-229:EGFR, LN-229:EGFRvIII, or LN-229:EGFR/EGFRvIII cells were serum-staved for 24 hr then treated with or without EGF (50 ng/ml) for 15 min prior

    Article Snippet: Sections were immunostained with antibodies against EGFR (mouse monoclonal DAK-H1-WT, Dako, diluted 1:200), EGFRvIII (rabbit polyclonal 6549, Celldex, diluted 1:5000), p-STAT3 (Tyr705) (rabbit monoclonal antibody D3A7 or mouse monoclonal antibody 3E2, Cell Signaling, each diluted 1:50).

    Techniques: Expressing

    EGF treatment of LN-229:EGFR/EGFRvIII cells leads to prolonged phosphorylation of EGFR, EGFRvIII, and STAT3/5. (A) LN-229:parent, LN-229:EGFR, LN-229:EGFRvIII, or LN-229:EGFR/EGFRvIII cells were serum-starved for 24 hr then treated with EGF (50 ng/ml)

    Journal: Cancer cell

    Article Title: EGFR phosphorylates tumor-derived EGFRvIII driving STAT3/5 and progression in glioblastoma

    doi: 10.1016/j.ccr.2013.09.004

    Figure Lengend Snippet: EGF treatment of LN-229:EGFR/EGFRvIII cells leads to prolonged phosphorylation of EGFR, EGFRvIII, and STAT3/5. (A) LN-229:parent, LN-229:EGFR, LN-229:EGFRvIII, or LN-229:EGFR/EGFRvIII cells were serum-starved for 24 hr then treated with EGF (50 ng/ml)

    Article Snippet: Sections were immunostained with antibodies against EGFR (mouse monoclonal DAK-H1-WT, Dako, diluted 1:200), EGFRvIII (rabbit polyclonal 6549, Celldex, diluted 1:5000), p-STAT3 (Tyr705) (rabbit monoclonal antibody D3A7 or mouse monoclonal antibody 3E2, Cell Signaling, each diluted 1:50).

    Techniques:

    EGFRvIII serves as a substrate for EGFR. LN-229 cells stably transduced with retroviral constructs indicated were serum-starved for 24 hr then treated with or without EGF (50 ng/ml) for 15 min prior to harvest. Lysates were immunoblotted using antisera

    Journal: Cancer cell

    Article Title: EGFR phosphorylates tumor-derived EGFRvIII driving STAT3/5 and progression in glioblastoma

    doi: 10.1016/j.ccr.2013.09.004

    Figure Lengend Snippet: EGFRvIII serves as a substrate for EGFR. LN-229 cells stably transduced with retroviral constructs indicated were serum-starved for 24 hr then treated with or without EGF (50 ng/ml) for 15 min prior to harvest. Lysates were immunoblotted using antisera

    Article Snippet: Sections were immunostained with antibodies against EGFR (mouse monoclonal DAK-H1-WT, Dako, diluted 1:200), EGFRvIII (rabbit polyclonal 6549, Celldex, diluted 1:5000), p-STAT3 (Tyr705) (rabbit monoclonal antibody D3A7 or mouse monoclonal antibody 3E2, Cell Signaling, each diluted 1:50).

    Techniques: Stable Transfection, Transduction, Construct

    Detection of EGFR and EGFRvIII in primary human glioblastoma. (A) Graphical analysis of immunohistochemical data from Table S1. (B-C) Immunohistochemical staining of a primary human GBM with EGFR-specific (top panel) or EGFRvIII-specific antibody (bottom

    Journal: Cancer cell

    Article Title: EGFR phosphorylates tumor-derived EGFRvIII driving STAT3/5 and progression in glioblastoma

    doi: 10.1016/j.ccr.2013.09.004

    Figure Lengend Snippet: Detection of EGFR and EGFRvIII in primary human glioblastoma. (A) Graphical analysis of immunohistochemical data from Table S1. (B-C) Immunohistochemical staining of a primary human GBM with EGFR-specific (top panel) or EGFRvIII-specific antibody (bottom

    Article Snippet: Sections were immunostained with antibodies against EGFR (mouse monoclonal DAK-H1-WT, Dako, diluted 1:200), EGFRvIII (rabbit polyclonal 6549, Celldex, diluted 1:5000), p-STAT3 (Tyr705) (rabbit monoclonal antibody D3A7 or mouse monoclonal antibody 3E2, Cell Signaling, each diluted 1:50).

    Techniques: Immunohistochemistry, Staining