mouse anti human cd8 antibody  (Agilent technologies)

 
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    Agilent technologies mouse anti human cd8 antibody
    Ex vivo PD-1 expression in skin and blood derived CD4 + and <t>CD8</t> + T cells
    Mouse Anti Human Cd8 Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human cd8 antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human cd8 antibody - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "The Characterization of Varicella Zoster Virus Specific T Cells In Skin and Blood During Ageing"

    Article Title: The Characterization of Varicella Zoster Virus Specific T Cells In Skin and Blood During Ageing

    Journal: The Journal of investigative dermatology

    doi: 10.1038/jid.2015.63

    Ex vivo PD-1 expression in skin and blood derived CD4 + and CD8 + T cells
    Figure Legend Snippet: Ex vivo PD-1 expression in skin and blood derived CD4 + and CD8 + T cells

    Techniques Used: Ex Vivo, Expressing, Derivative Assay

    2) Product Images from "Natural killer cells in the human lung tumor microenvironment display immune inhibitory functions"

    Article Title: Natural killer cells in the human lung tumor microenvironment display immune inhibitory functions

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1136/jitc-2020-001054

    Clinical impact of NKp46 + cells in patients with non-small cell lung carcinoma. (A, B) Patients from the retrospective cohort (cohort 3) were splitted into two groups according to the density of intratumoral NKp46 + cells (A) or CD8 + cells (B). Separation was done by median and their overall survival was analyzed. (C) Patients with low density (CD8 + cells Low ) or high density (CD8 + cells High ) of CD8 + cells were splitted into two groups according to their number of intratumoral Nkp46 + cells and the overall survival were done in each group. Separation was done by median. (D, E) CD8 density of patients from the validation cohort (cohort 2) was assessed by immunohistochemistry on paraffin-embedded slides and the correlation with cytotoxic T-lymphocyte-associated protein 4 (CTLA4) mRNA expression in natural killer (NK) cells was calculated using Pearson correlation test with the GraphPad software (D). (E) Patients from validation cohort (cohort 2) were split in two groups according to the density of intratumoral CD8 + cells (using median) and the expression of CTLA4 mRNA was analyzed in each group. Statistical analyses were performed by the Mann-Whitney non-parametric test with the GraphPad software.
    Figure Legend Snippet: Clinical impact of NKp46 + cells in patients with non-small cell lung carcinoma. (A, B) Patients from the retrospective cohort (cohort 3) were splitted into two groups according to the density of intratumoral NKp46 + cells (A) or CD8 + cells (B). Separation was done by median and their overall survival was analyzed. (C) Patients with low density (CD8 + cells Low ) or high density (CD8 + cells High ) of CD8 + cells were splitted into two groups according to their number of intratumoral Nkp46 + cells and the overall survival were done in each group. Separation was done by median. (D, E) CD8 density of patients from the validation cohort (cohort 2) was assessed by immunohistochemistry on paraffin-embedded slides and the correlation with cytotoxic T-lymphocyte-associated protein 4 (CTLA4) mRNA expression in natural killer (NK) cells was calculated using Pearson correlation test with the GraphPad software (D). (E) Patients from validation cohort (cohort 2) were split in two groups according to the density of intratumoral CD8 + cells (using median) and the expression of CTLA4 mRNA was analyzed in each group. Statistical analyses were performed by the Mann-Whitney non-parametric test with the GraphPad software.

    Techniques Used: Immunohistochemistry, Expressing, Software, MANN-WHITNEY

    3) Product Images from "Expression of the costimulatory molecule B7-H3 is associated with prolonged survival in human pancreatic cancer"

    Article Title: Expression of the costimulatory molecule B7-H3 is associated with prolonged survival in human pancreatic cancer

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-9-463

    Representative immunohistochemical stainings for B7-H3 (A, C) and CD8 (B, D) in pancreatic cancer tissues . (A) Pancreatic cancer tissue section with strong B7-H3 immunoreactivity. (B) Consecutive section with immunostaining for CD8 shows the infiltration of numerous CD8+ T cells (arrows). (C) Pancreatic cancer tissue section with weak tumor B7-H3 immunoreactivity. (D) Consecutive section with immunostaining for CD8 shows no CD8+ tumor-infiltrating T cells.
    Figure Legend Snippet: Representative immunohistochemical stainings for B7-H3 (A, C) and CD8 (B, D) in pancreatic cancer tissues . (A) Pancreatic cancer tissue section with strong B7-H3 immunoreactivity. (B) Consecutive section with immunostaining for CD8 shows the infiltration of numerous CD8+ T cells (arrows). (C) Pancreatic cancer tissue section with weak tumor B7-H3 immunoreactivity. (D) Consecutive section with immunostaining for CD8 shows no CD8+ tumor-infiltrating T cells.

    Techniques Used: Immunohistochemistry, Immunostaining

    Semi-quantitative analysis of CD8+ T cells in pancreatic cancer . In areas with high tumor B7-H3 expression, the prevalence of CD8+ T cells was significantly increased compared to areas with low tumor B7-H3 expression (p = 0.018).
    Figure Legend Snippet: Semi-quantitative analysis of CD8+ T cells in pancreatic cancer . In areas with high tumor B7-H3 expression, the prevalence of CD8+ T cells was significantly increased compared to areas with low tumor B7-H3 expression (p = 0.018).

    Techniques Used: Expressing

    4) Product Images from "Epigenetic silencing of Th1 type chemokines shapes tumor immunity and immunotherapy"

    Article Title: Epigenetic silencing of Th1 type chemokines shapes tumor immunity and immunotherapy

    Journal: Nature

    doi: 10.1038/nature15520

    Epigenetic reprogramming alters immunotherapy a–c , Effects of DZNep and 5-AZA dC on ID8 mouse ovarian cancer progression. The ID8 tumor bearing mice (C57BL/6) were treated with DZNep and 5-AZA dC. ( a ) Tumor growth was recorded by Bioluminescence imaging and quantified by calculating the total flux (photons per second). The representative images and tumor volume at day 24 are shown. Day 0: tumor inoculation. ( b ) Tumor-infiltrating CD8 + T cells were quantified by immunohistochemistry staining (IHC) and expressed as the mean ± SEM per high-power field. ( c ) Tumor CXCL9 mRNA was quantified by real-time PCR. (mean/SEM, n = 5 per group, * P
    Figure Legend Snippet: Epigenetic reprogramming alters immunotherapy a–c , Effects of DZNep and 5-AZA dC on ID8 mouse ovarian cancer progression. The ID8 tumor bearing mice (C57BL/6) were treated with DZNep and 5-AZA dC. ( a ) Tumor growth was recorded by Bioluminescence imaging and quantified by calculating the total flux (photons per second). The representative images and tumor volume at day 24 are shown. Day 0: tumor inoculation. ( b ) Tumor-infiltrating CD8 + T cells were quantified by immunohistochemistry staining (IHC) and expressed as the mean ± SEM per high-power field. ( c ) Tumor CXCL9 mRNA was quantified by real-time PCR. (mean/SEM, n = 5 per group, * P

    Techniques Used: Mouse Assay, Imaging, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction

    EZH2/H3K27 and DNMT1 interaction affects clinical outcome a–b, Representative images of immunohistochemistry staining of EZH2 ( a ) and DNMT1 ( b ) in human ovarian cancer tissues. The levels of DNMT1 and EZH2 expression in the tumor were assessed by H-score method. c–d, The association between EZH2 ( c ), DNMT1 ( d ) and patient disease free survival in high-grade serous ovarian cancer. The high and low levels of EZH2 and DNMT1 were determined by the median values (see Extended Data Table 1 ). e, Relative impact of EZH2, DNMT1 and CD8 on patient disease free survival in high-grade serous ovarian cancer. The time-dependent receiver operating characteristic (ROC) curve analysis was applied to evaluate the predictive accuracy of each marker for disease free survival. AUC, the area under the ROC curve. (T = 60). f, Impact of the two parameters (EZH2 and DNMT1) on patient disease free survival. The analysis was performed on patients with high-grade serous ovarian cancer. Multiple comparisons were performed in long-rank test. EZH2 low DNMT1 low group (n = 49) vs EZH2 high DNMT1 high (n = 55) P
    Figure Legend Snippet: EZH2/H3K27 and DNMT1 interaction affects clinical outcome a–b, Representative images of immunohistochemistry staining of EZH2 ( a ) and DNMT1 ( b ) in human ovarian cancer tissues. The levels of DNMT1 and EZH2 expression in the tumor were assessed by H-score method. c–d, The association between EZH2 ( c ), DNMT1 ( d ) and patient disease free survival in high-grade serous ovarian cancer. The high and low levels of EZH2 and DNMT1 were determined by the median values (see Extended Data Table 1 ). e, Relative impact of EZH2, DNMT1 and CD8 on patient disease free survival in high-grade serous ovarian cancer. The time-dependent receiver operating characteristic (ROC) curve analysis was applied to evaluate the predictive accuracy of each marker for disease free survival. AUC, the area under the ROC curve. (T = 60). f, Impact of the two parameters (EZH2 and DNMT1) on patient disease free survival. The analysis was performed on patients with high-grade serous ovarian cancer. Multiple comparisons were performed in long-rank test. EZH2 low DNMT1 low group (n = 49) vs EZH2 high DNMT1 high (n = 55) P

    Techniques Used: Immunohistochemistry, Staining, Expressing, Marker

    Epigenetic reprogramming alters cancer T cell immunotherapy Effects of GSK126 and 5-AZA dC on T cell immunotherapy. Ovarian cancer-bearing NSG mice were treated with GSK126, 5-AZA dC and/or anti-CXCR3, and received TAA-specific CD8 + T cell transfusion. Tumor volume ( a ), tumor chemokine expression ( b, c ), tumor-infiltrating T cells and blood T cells ( d, e ) were shown. CXCL10 transcript was quantified in tumor and immune cells from tumor tissues in NSG model ( f ). Tumor and immune cells (10 6 /ml) were isolated from ovarian cancer from patients, and were treated for 48 hours in the indicated conditions. CXCL10 protein was measured by ELISA ( g ) (mean/SD, n = 5, * P
    Figure Legend Snippet: Epigenetic reprogramming alters cancer T cell immunotherapy Effects of GSK126 and 5-AZA dC on T cell immunotherapy. Ovarian cancer-bearing NSG mice were treated with GSK126, 5-AZA dC and/or anti-CXCR3, and received TAA-specific CD8 + T cell transfusion. Tumor volume ( a ), tumor chemokine expression ( b, c ), tumor-infiltrating T cells and blood T cells ( d, e ) were shown. CXCL10 transcript was quantified in tumor and immune cells from tumor tissues in NSG model ( f ). Tumor and immune cells (10 6 /ml) were isolated from ovarian cancer from patients, and were treated for 48 hours in the indicated conditions. CXCL10 protein was measured by ELISA ( g ) (mean/SD, n = 5, * P

    Techniques Used: Mouse Assay, Expressing, Isolation, Enzyme-linked Immunosorbent Assay

    5) Product Images from "Evolution of neoantigen landscape during immune checkpoint blockade in non-small cell lung cancer"

    Article Title: Evolution of neoantigen landscape during immune checkpoint blockade in non-small cell lung cancer

    Journal: Cancer discovery

    doi: 10.1158/2159-8290.CD-16-0828

    Emergence of resistance to immune checkpoint blockade is associated with elimination of mutation associated neoantigens by loss of heterozygosity and a more diverse T-cell repertoire independent of PD-L1 expression Panel A shows computed tomographic (CT) images of patient CGLU117 at baseline, at the time of therapeutic response and at time of acquired resistance. Pre-treatment CT image of the abdomen, demonstrates a right adrenal mass (T1, circled), radiologic tumor regression is noted after 2 months of treatment, followed by disease relapse at 4 months from treatment initiation with a markedly increased right adrenal metastasis (T2, circled). 3 rd follow up CT demonstrates further disease progression in the adrenal lesion. Tumor burden kinetics for target lesions by RECIST criteria are shown in panel B. Peripheral T cell expansion of a subset of intratumoral clones was noted to peak at the time of response and decrease to baseline levels at the time of resistance (panel C). Productive TCR frequency denotes the frequency of a specific rearrangement that can produce a functional protein receptor among all productive rearrangements. Panels D and E show B allele frequency graphs for chromosome 17, a value of 0.5 indicates a heterozygous genotype whereas allelic imbalance is observed as a deviation from 0.5. The region that undergoes LOH in the resistant tumor (panel E, orange box) contains 3 mutation associated neoantigens that are thus eliminated. No differences in CD8+ T cell density (panel F, G) or PD-L1 expression (panel H, I) were observed between baseline and resistant tumors.
    Figure Legend Snippet: Emergence of resistance to immune checkpoint blockade is associated with elimination of mutation associated neoantigens by loss of heterozygosity and a more diverse T-cell repertoire independent of PD-L1 expression Panel A shows computed tomographic (CT) images of patient CGLU117 at baseline, at the time of therapeutic response and at time of acquired resistance. Pre-treatment CT image of the abdomen, demonstrates a right adrenal mass (T1, circled), radiologic tumor regression is noted after 2 months of treatment, followed by disease relapse at 4 months from treatment initiation with a markedly increased right adrenal metastasis (T2, circled). 3 rd follow up CT demonstrates further disease progression in the adrenal lesion. Tumor burden kinetics for target lesions by RECIST criteria are shown in panel B. Peripheral T cell expansion of a subset of intratumoral clones was noted to peak at the time of response and decrease to baseline levels at the time of resistance (panel C). Productive TCR frequency denotes the frequency of a specific rearrangement that can produce a functional protein receptor among all productive rearrangements. Panels D and E show B allele frequency graphs for chromosome 17, a value of 0.5 indicates a heterozygous genotype whereas allelic imbalance is observed as a deviation from 0.5. The region that undergoes LOH in the resistant tumor (panel E, orange box) contains 3 mutation associated neoantigens that are thus eliminated. No differences in CD8+ T cell density (panel F, G) or PD-L1 expression (panel H, I) were observed between baseline and resistant tumors.

    Techniques Used: Mutagenesis, Expressing, Clone Assay, Functional Assay

    Related Articles

    Incubation:

    Article Title: Evolution of neoantigen landscape during immune checkpoint blockade in non-small cell lung cancer
    Article Snippet: Samples with membranous PD-L1 staining with an intensity score of 2+ in at least 1% of cells were classified as PD-L1 positive. .. Similarly, slides were deparaffinized, rehydrated, antigen retrieved and incubated with a mouse anti-human CD8 antibody (Dako, CA) diluted 1:100 overnight at 4°C, followed by a 30-minute incubation with the FLEX+ polymer system. ..

    Staining:

    Article Title: Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction
    Article Snippet: Paraffin-embedded TMA sections were deparaffinized and stained with rabbit anti-human EZH2 (1:200, 187395, Invitrogen) and followed by Polymer-HRP and DAB Chromogen treatment. .. TMA sections were subsequently stained with mouse anti-human CD8 (1:50, M7103, Dako), followed by treatment with Rabbit-Mouse LINK, Polymer-AP, Permanent Red Chromogen and hematoxylin counterstaining. .. The TMA cores were quantified and analyzed for expression of EZH2+ and CD8+ T cells with an Aperio imaging system (Genetix).

    Article Title: Decreased TNF-α synthesis by macrophages restricts cutaneous immunosurveillance by memory CD4+ T cells during aging
    Article Snippet: .. Skin sections from normal and C. albicans –injected skin (n = 25 young, 26 old) were stained with optimal dilutions of purified mouse anti–human CD3 antibody (Dako), purified mouse anti–human CD4 antibody (BD), purified mouse anti–human CD8 antibody (Dako), purified mouse anti–human CD163 antibody (Acris), and anti–TNF-α FITC (BD). .. Rabbit anti–mouse horseradish peroxidase–conjugated antibody (Dako) was added to detect anti-CD3, -CD4, and -CD8.

    Injection:

    Article Title: Decreased TNF-α synthesis by macrophages restricts cutaneous immunosurveillance by memory CD4+ T cells during aging
    Article Snippet: .. Skin sections from normal and C. albicans –injected skin (n = 25 young, 26 old) were stained with optimal dilutions of purified mouse anti–human CD3 antibody (Dako), purified mouse anti–human CD4 antibody (BD), purified mouse anti–human CD8 antibody (Dako), purified mouse anti–human CD163 antibody (Acris), and anti–TNF-α FITC (BD). .. Rabbit anti–mouse horseradish peroxidase–conjugated antibody (Dako) was added to detect anti-CD3, -CD4, and -CD8.

    Purification:

    Article Title: Decreased TNF-α synthesis by macrophages restricts cutaneous immunosurveillance by memory CD4+ T cells during aging
    Article Snippet: .. Skin sections from normal and C. albicans –injected skin (n = 25 young, 26 old) were stained with optimal dilutions of purified mouse anti–human CD3 antibody (Dako), purified mouse anti–human CD4 antibody (BD), purified mouse anti–human CD8 antibody (Dako), purified mouse anti–human CD163 antibody (Acris), and anti–TNF-α FITC (BD). .. Rabbit anti–mouse horseradish peroxidase–conjugated antibody (Dako) was added to detect anti-CD3, -CD4, and -CD8.

    Immunohistochemistry:

    Article Title: Regulation of Pulmonary Graft-versus-Host Disease by IL-26+CD26+CD4 T Lymphocytes
    Article Snippet: For detection of collagen, slides were stained with Azan-Mallory staining. .. Abs used in immunohistochemistry were anti-human CD4 mAb (4B12, mouse IgG1) and anti-human CD8 mAb (C8/144B, mouse IgG1) from Dako (Tokyo, Japan), anti-human CD26 goat pAb (AF1180) from R & D Systems, anti–IL-26 rabbit pAb (bs-2626R) from Bioss (Wobum, MA), and anti–IL-17A rabbit pAb (H-132) from Santa Cruz Biotechnology (Santa Cruz, CA). .. Immunohistochemical staining for CD4, CD8, CD26, or IL-26 was performed at the Department of Pathology, Keio University School of Medicine (Tokyo, Japan), as described previously ( ).

    Immunodetection:

    Article Title: Natural killer cells in the human lung tumor microenvironment display immune inhibitory functions
    Article Snippet: Heat-mediated antigen retrieval was performed using the EnVision FLEX Target Retrieval Solutions (Agilent, Dako, California, USA) at pH9 for 30 min on a PT-Link (Dako). .. Immunodetection of CD8 expression was done using a mouse anti-human CD8 antibody (Clone C8/144B). (Dako) at 1.6 µg/mL for 30 min in Dako REAL Antibody Diluent (Dako). .. Signal intensity was improved with EnVision +System-HRP-labeled Polymers anti-mouse (Dako) and peroxidase was detected using diaminobenzidine +Substrate—Chromogen System (Dako).

    Expressing:

    Article Title: Natural killer cells in the human lung tumor microenvironment display immune inhibitory functions
    Article Snippet: Heat-mediated antigen retrieval was performed using the EnVision FLEX Target Retrieval Solutions (Agilent, Dako, California, USA) at pH9 for 30 min on a PT-Link (Dako). .. Immunodetection of CD8 expression was done using a mouse anti-human CD8 antibody (Clone C8/144B). (Dako) at 1.6 µg/mL for 30 min in Dako REAL Antibody Diluent (Dako). .. Signal intensity was improved with EnVision +System-HRP-labeled Polymers anti-mouse (Dako) and peroxidase was detected using diaminobenzidine +Substrate—Chromogen System (Dako).

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    • mouse anti human cd8 antibodies  (Agilent technologies)
    86
    Agilent technologies mouse anti human cd8 antibodies
    Lymphocytes traffic to peripheral lymphoid tissues after adoptive transfer. A Diagram displaying the transfer protocol. B Donor bulk PBMC were transferred from MCM7 into MCM6. MCM6 was euthanized 24 hours post transfer and its tissues were processed. Cells selectively trafficked to different peripheral lymphoid organs post-transfer. C Immunohistochemical staining of a lymph node section showing PKH67+ cells (green), CD20 antibody staining (red) and <t>CD8</t> antibody staining (blue) collected with a confocal microscope at 200X.
    Mouse Anti Human Cd8 Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human cd8 antibodies/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human cd8 antibodies - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    mouse anti human cd8 mab  (Agilent technologies)
    86
    Agilent technologies mouse anti human cd8 mab
    Correlation of the number of <t>CD8</t> + T cells before and after NAC with the HMGB1 and calreticulin score, and patient survival in breast cancer patients. (A) Evaluation of the number of CD8 + T cells before and after NAC. Representative IHC of CD8 is shown. (B) Evaluation of the number of CD8 + T cells before and after NAC in pathological response. (C) Correlation of the HMGB1/calreticulin score with the number of CD8 + T cells before and after NAC. (D) Correlation of the degree of infiltrating CD8 + T cells before and after NAC with clinical outcomes. *p
    Mouse Anti Human Cd8 Mab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human cd8 mab/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human cd8 mab - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    monoclonal mouse anti human cd8  (Agilent technologies)
    86
    Agilent technologies monoclonal mouse anti human cd8
    Representative features of histologic and immunohistochemical analyses Histologic features of invasive oral squamous cell carcinomas are shown (hematoxylin and eosin stain, x200; A, B, C ). Immunostaining of PD-L1 revealed no staining (0; D ), weak positivity (1+; E ), and moderate to strong positivity (2+; F ). Tumor-infiltrating lymphocytes tended to be variably correlated with PD-L1 expression. The infiltrating lymphoid cells were immunostained with CD3 (G, H, I) , CD4 (J, K, L) , <t>CD8</t> (M, N, O) , PD-1 (P, Q, R) , FoxP3 (S, T, U) , and CD20 (V, W, X) .
    Monoclonal Mouse Anti Human Cd8, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human cd8/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti human cd8 - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier
    anti cd8  (Agilent technologies)
    99
    Agilent technologies anti cd8
    Accumulation of <t>CD8+</t> T-lymphocytes in tumor regions with expression of cFTH1. A , IHC staining of FTH1, CD3, CD4 and CD8 in TNBC whole tissue sections (yellow arrows: CD4+ T-lymphocytes; red arrows: CD8+ T-lymphocytes); B , Box plots summarize the observation in (A). CD4+/CD8+ ratio was significantly increased in TNBC samples with high expression of cFTH1. Data are presented as the median ± interquartile range (Mann-Whitney U test, n = 15 per group); C , A proposed functional model of cFTH1 in the context of the antigen processing and presentation pathway: Ferritin complex captures intracellular Fe 2+ ions (green dots) and converts them into Fe 3+ ions (yellow dots). Accumulation of Fe 3+ iron in tumor cells increases in response to inflammation, and IFN γ (red dots) can enhance the inflammatory response of tumor cells. Together, inflammatory signals can enhance processing of cytosolic antigens through the proteasome, heat shock proteins (HSP), and antigen peptide transporters (TAP), resulting in antigen presentation by major histocompatibility complex class I (MHC I). This in turn attracts CD8+ T-lymphocytes that recognize tumor cells and induce the apoptotic process. On the other hand, cFTH1 can be transported from the cytoplasm to the nucleus. nFTH1 protects from DNA damage caused by reactive oxygen species (ROS) (dashed arrow). Solid lines: observed interactions; Dashed line: hypothetical events; Green arrows: molecular functions of cFTH1; Red arrow: molecular function of nFTH1; Black arrows: pathways driven by FTH1.
    Anti Cd8, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8/product/Agilent technologies
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    anti cd8 - by Bioz Stars, 2021-04
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    Image Search Results


    Lymphocytes traffic to peripheral lymphoid tissues after adoptive transfer. A Diagram displaying the transfer protocol. B Donor bulk PBMC were transferred from MCM7 into MCM6. MCM6 was euthanized 24 hours post transfer and its tissues were processed. Cells selectively trafficked to different peripheral lymphoid organs post-transfer. C Immunohistochemical staining of a lymph node section showing PKH67+ cells (green), CD20 antibody staining (red) and CD8 antibody staining (blue) collected with a confocal microscope at 200X.

    Journal: PLoS ONE

    Article Title: Allogeneic Lymphocytes Persist and Traffic in Feral MHC-Matched Mauritian Cynomolgus Macaques

    doi: 10.1371/journal.pone.0002384

    Figure Lengend Snippet: Lymphocytes traffic to peripheral lymphoid tissues after adoptive transfer. A Diagram displaying the transfer protocol. B Donor bulk PBMC were transferred from MCM7 into MCM6. MCM6 was euthanized 24 hours post transfer and its tissues were processed. Cells selectively trafficked to different peripheral lymphoid organs post-transfer. C Immunohistochemical staining of a lymph node section showing PKH67+ cells (green), CD20 antibody staining (red) and CD8 antibody staining (blue) collected with a confocal microscope at 200X.

    Article Snippet: Free floating sections were incubated overnight on a rocking platform at 4°C with purified mouse-anti-human CD8 antibodies (Dako) diluted 1∶200 in a blocking solution of PBS-H containing 2% normal goat serum.

    Techniques: Adoptive Transfer Assay, Immunohistochemistry, Staining, Microscopy

    Correlation of the number of CD8 + T cells before and after NAC with the HMGB1 and calreticulin score, and patient survival in breast cancer patients. (A) Evaluation of the number of CD8 + T cells before and after NAC. Representative IHC of CD8 is shown. (B) Evaluation of the number of CD8 + T cells before and after NAC in pathological response. (C) Correlation of the HMGB1/calreticulin score with the number of CD8 + T cells before and after NAC. (D) Correlation of the degree of infiltrating CD8 + T cells before and after NAC with clinical outcomes. *p

    Journal: Oncology Reports

    Article Title: Immunogenic tumor cell death induced by chemotherapy in patients with breast cancer and esophageal squamous cell carcinoma

    doi: 10.3892/or.2017.6097

    Figure Lengend Snippet: Correlation of the number of CD8 + T cells before and after NAC with the HMGB1 and calreticulin score, and patient survival in breast cancer patients. (A) Evaluation of the number of CD8 + T cells before and after NAC. Representative IHC of CD8 is shown. (B) Evaluation of the number of CD8 + T cells before and after NAC in pathological response. (C) Correlation of the HMGB1/calreticulin score with the number of CD8 + T cells before and after NAC. (D) Correlation of the degree of infiltrating CD8 + T cells before and after NAC with clinical outcomes. *p

    Article Snippet: Thereafter, the sections were incubated with diluted normal blocking serum for 20 min and incubated with one of the following: mouse anti-human HMGB1 mAb (cat. no. SAB1403925, clone 2F6, 3 mg/ml; Sigma-Aldrich, Tokyo, Japan) overnight at 4°C; mouse anti-human calreticulin mAb (cat. no. ab22683, 5 mg/ml; Abcam, Cambridge, UK) for 2 h at 37°C; or mouse anti-human CD8 mAb (cat. no. M7103, 1.6 µg/ml; Dako) overnight at 4°C.

    Techniques: Immunohistochemistry

    Representative features of histologic and immunohistochemical analyses Histologic features of invasive oral squamous cell carcinomas are shown (hematoxylin and eosin stain, x200; A, B, C ). Immunostaining of PD-L1 revealed no staining (0; D ), weak positivity (1+; E ), and moderate to strong positivity (2+; F ). Tumor-infiltrating lymphocytes tended to be variably correlated with PD-L1 expression. The infiltrating lymphoid cells were immunostained with CD3 (G, H, I) , CD4 (J, K, L) , CD8 (M, N, O) , PD-1 (P, Q, R) , FoxP3 (S, T, U) , and CD20 (V, W, X) .

    Journal: Oncotarget

    Article Title: Clinicopathologic implications of the miR-197/PD-L1 axis in oral squamous cell carcinoma

    doi: 10.18632/oncotarget.19842

    Figure Lengend Snippet: Representative features of histologic and immunohistochemical analyses Histologic features of invasive oral squamous cell carcinomas are shown (hematoxylin and eosin stain, x200; A, B, C ). Immunostaining of PD-L1 revealed no staining (0; D ), weak positivity (1+; E ), and moderate to strong positivity (2+; F ). Tumor-infiltrating lymphocytes tended to be variably correlated with PD-L1 expression. The infiltrating lymphoid cells were immunostained with CD3 (G, H, I) , CD4 (J, K, L) , CD8 (M, N, O) , PD-1 (P, Q, R) , FoxP3 (S, T, U) , and CD20 (V, W, X) .

    Article Snippet: The following primary antibodies were used: polyclonal rabbit anti-human PD-L1 (clone ab153991, Abcam, Cambridge, UK; 1:1000), monoclonal rabbit anti-human PD-1 (clone #6796-1, Epitomics, Burlingame, CA, USA; 1:300), monoclonal rabbit anti-human CD4 (clone SP35, Catalog no. 790-4423, Ventana Medical Systems), monoclonal mouse anti-human CD8 (clone C8/144B, Catalog no. IR623, Dako, Carpinteria, CA, USA), mouse monoclonal anti-human FoxP3 (clone #236A/E7, Catalog no. ab20034, Abcam; 1:50), rabbit monoclonal anti-human CD3 (clone #SP7, Catalog no. RM-9107-S, Thermo Scientific, Waltham, MA, USA; 1:100), mouse monoclonal anti-human CD20 (clone #L26, Catalog no. IR604, Dako).

    Techniques: Immunohistochemistry, H&E Stain, Immunostaining, Staining, Expressing

    Dot plots of relative miR-197 expression level and tumor-infiltrating lymphocytes (TILs) according to PD-L1 expression and T stages MiR-197 expression level (A) and the numbers of CD3+ (B) , CD4+ (C) , CD8+ (D) , PD-1+ (E) , FoxP3+ (F) , and CD20+ (G) tumor-infiltrating lymphocytes (TILs) were illustrated according to PD-L1 level. PD-L1 expression is inversely correlated with miR-197 level (A) but tended to have a positive correlation with TILs (B–G). MiR-197 level is higher in T4 disease than in T1-T3 disease (H) .

    Journal: Oncotarget

    Article Title: Clinicopathologic implications of the miR-197/PD-L1 axis in oral squamous cell carcinoma

    doi: 10.18632/oncotarget.19842

    Figure Lengend Snippet: Dot plots of relative miR-197 expression level and tumor-infiltrating lymphocytes (TILs) according to PD-L1 expression and T stages MiR-197 expression level (A) and the numbers of CD3+ (B) , CD4+ (C) , CD8+ (D) , PD-1+ (E) , FoxP3+ (F) , and CD20+ (G) tumor-infiltrating lymphocytes (TILs) were illustrated according to PD-L1 level. PD-L1 expression is inversely correlated with miR-197 level (A) but tended to have a positive correlation with TILs (B–G). MiR-197 level is higher in T4 disease than in T1-T3 disease (H) .

    Article Snippet: The following primary antibodies were used: polyclonal rabbit anti-human PD-L1 (clone ab153991, Abcam, Cambridge, UK; 1:1000), monoclonal rabbit anti-human PD-1 (clone #6796-1, Epitomics, Burlingame, CA, USA; 1:300), monoclonal rabbit anti-human CD4 (clone SP35, Catalog no. 790-4423, Ventana Medical Systems), monoclonal mouse anti-human CD8 (clone C8/144B, Catalog no. IR623, Dako, Carpinteria, CA, USA), mouse monoclonal anti-human FoxP3 (clone #236A/E7, Catalog no. ab20034, Abcam; 1:50), rabbit monoclonal anti-human CD3 (clone #SP7, Catalog no. RM-9107-S, Thermo Scientific, Waltham, MA, USA; 1:100), mouse monoclonal anti-human CD20 (clone #L26, Catalog no. IR604, Dako).

    Techniques: Expressing

    Accumulation of CD8+ T-lymphocytes in tumor regions with expression of cFTH1. A , IHC staining of FTH1, CD3, CD4 and CD8 in TNBC whole tissue sections (yellow arrows: CD4+ T-lymphocytes; red arrows: CD8+ T-lymphocytes); B , Box plots summarize the observation in (A). CD4+/CD8+ ratio was significantly increased in TNBC samples with high expression of cFTH1. Data are presented as the median ± interquartile range (Mann-Whitney U test, n = 15 per group); C , A proposed functional model of cFTH1 in the context of the antigen processing and presentation pathway: Ferritin complex captures intracellular Fe 2+ ions (green dots) and converts them into Fe 3+ ions (yellow dots). Accumulation of Fe 3+ iron in tumor cells increases in response to inflammation, and IFN γ (red dots) can enhance the inflammatory response of tumor cells. Together, inflammatory signals can enhance processing of cytosolic antigens through the proteasome, heat shock proteins (HSP), and antigen peptide transporters (TAP), resulting in antigen presentation by major histocompatibility complex class I (MHC I). This in turn attracts CD8+ T-lymphocytes that recognize tumor cells and induce the apoptotic process. On the other hand, cFTH1 can be transported from the cytoplasm to the nucleus. nFTH1 protects from DNA damage caused by reactive oxygen species (ROS) (dashed arrow). Solid lines: observed interactions; Dashed line: hypothetical events; Green arrows: molecular functions of cFTH1; Red arrow: molecular function of nFTH1; Black arrows: pathways driven by FTH1.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Ferritin Heavy Chain in Triple Negative Breast Cancer: A Favorable Prognostic Marker that Relates to a Cluster of Differentiation 8 Positive (CD8+) Effector T-cell Response *

    doi: 10.1074/mcp.M113.037176

    Figure Lengend Snippet: Accumulation of CD8+ T-lymphocytes in tumor regions with expression of cFTH1. A , IHC staining of FTH1, CD3, CD4 and CD8 in TNBC whole tissue sections (yellow arrows: CD4+ T-lymphocytes; red arrows: CD8+ T-lymphocytes); B , Box plots summarize the observation in (A). CD4+/CD8+ ratio was significantly increased in TNBC samples with high expression of cFTH1. Data are presented as the median ± interquartile range (Mann-Whitney U test, n = 15 per group); C , A proposed functional model of cFTH1 in the context of the antigen processing and presentation pathway: Ferritin complex captures intracellular Fe 2+ ions (green dots) and converts them into Fe 3+ ions (yellow dots). Accumulation of Fe 3+ iron in tumor cells increases in response to inflammation, and IFN γ (red dots) can enhance the inflammatory response of tumor cells. Together, inflammatory signals can enhance processing of cytosolic antigens through the proteasome, heat shock proteins (HSP), and antigen peptide transporters (TAP), resulting in antigen presentation by major histocompatibility complex class I (MHC I). This in turn attracts CD8+ T-lymphocytes that recognize tumor cells and induce the apoptotic process. On the other hand, cFTH1 can be transported from the cytoplasm to the nucleus. nFTH1 protects from DNA damage caused by reactive oxygen species (ROS) (dashed arrow). Solid lines: observed interactions; Dashed line: hypothetical events; Green arrows: molecular functions of cFTH1; Red arrow: molecular function of nFTH1; Black arrows: pathways driven by FTH1.

    Article Snippet: Anti-CD3 (rabbit-anti-human polyclonal antibody) and anti-CD8 (mouse-anti-human monoclonal antibody, clone name: C8/C144B) were purchased from Dako Denmark A/S (Glostrup, Denmark).

    Techniques: Expressing, Immunohistochemistry, Staining, MANN-WHITNEY, Functional Assay