Journal: Molecular and cellular endocrinology

Article Title: DUAL INHIBITION OF ERK1/2 AND AKT PATHWAYS IS REQUIRED TO SUPPRESS THE GROWTH AND SURVIVAL OF ENDOMETRIOTIC CELLS AND LESIONS

doi: 10.1016/j.mce.2018.12.011

Figure Lengend Snippet: Details of the antibody used.

Article Snippet: Anti-human mouse monoclonal Cyclin D3 , Cell Signaling , 2936 , 1:1000.

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Downregulated miR-195 Detected in Preeclamptic Placenta Affects Trophoblast Cell Invasion via Modulating ActRIIA Expression

doi: 10.1371/journal.pone.0038875

Figure Lengend Snippet: A. Real-time PCR to reveal miR-195 level in HTR8/SVneo cells transfected with scramble siRNA (NC) or miR-195 mimics (miR-195). **, compared with NC, p <0.01. B. Western blot analysis to show change of CCND1 and CCND3 protein level in HTR8/SVneo cells transfected with NC or miR-195. Upper panels, typical results of Western blotting; Lower panels, bar charts representing the statistical analysis by ANOVA according to three independent experiments. The densities of CCND1 and CCND3 were adjusted by that of Actin in the same blot, and the values were presented as Mean±SEM.

Article Snippet: The antibodies used included goat anti-human ActRIIA (R&D, Minneapolis, USA), rabbit anti-human CCND1 (Abcam, Cambridge, UK), mouse anti-human CCND3 (Cell Signaling Technology, Beverly, MA, USA), mouse anti-human Actin (Cell Signaling Technology, Beverly, MA, USA), and mouse anti-human GAPDH (Ambion, Austin, Texas, USA).

Techniques: Real-time Polymerase Chain Reaction, Transfection, Western Blot

Journal: Cancer gene therapy

Article Title: Intra-Tumoral Delivery of shRNA Targeting Cyclin D1 Attenuates Pancreatic Cancer Growth

doi: 10.1038/cgt.2009.75

Figure Lengend Snippet: ASPC-1 (a) and BxPC3 (b) cells that were either not infected (parental cells: P) or infected with the mock-infected lentivirus pll37 (M), or with pll37 containing sequences encoding luciferase (L), cyclin D1 specific shRNA 8 (8) or 10 (10), or their combination (K). Cell lysates, prepared 72 h after infection, were subjected to immunoblotting using anti-cyclin D1 antibodies. Membranes were re-probed with anti-tubulin antibody to assess equivalent lane loading.

Article Snippet: Briefly, PVDF membranes were incubated overnight with a 1:1000 dilution of anti-cyclin D1 human anti-mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA), washed, and incubated for 30 minutes with a secondary horseradish peroxidase-conjugated antibody.

Techniques: Infection, Luciferase, shRNA, Western Blot

Journal: Cancer gene therapy

Article Title: Intra-Tumoral Delivery of shRNA Targeting Cyclin D1 Attenuates Pancreatic Cancer Growth

doi: 10.1038/cgt.2009.75

Figure Lengend Snippet: ASPC-1 and BxPC3 cells that were either not infected (parental cells: P) or infected with the mock-infected lentivirus pll37 (E), or pll37 containing sequences encoding luciferase (L), or cyclin D1 specific shRNA 8 (8) or 10 (10), or their combination (K). (a) Anchorage-dependent growth was determined by the MTT assay. Data are the means ± SE of triplicate determinations from three independent experiments. *p<0.05, **p<0.01 when compared with Luc. ( b) Anchorage-independent growth was determined in soft agar. Data are the means ± SE of triplicate determinations from three independent experiments. **p<0.01 when compared with Luc. ( c) Cell invasion was determined in Transwell chambers. Data are the means ± SE of triplicate determinations from three independent experiments. **p<0.01 when compared with Luc.

Article Snippet: Briefly, PVDF membranes were incubated overnight with a 1:1000 dilution of anti-cyclin D1 human anti-mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA), washed, and incubated for 30 minutes with a secondary horseradish peroxidase-conjugated antibody.

Techniques: Infection, Luciferase, shRNA, MTT Assay

Journal: Cancer gene therapy

Article Title: Intra-Tumoral Delivery of shRNA Targeting Cyclin D1 Attenuates Pancreatic Cancer Growth

doi: 10.1038/cgt.2009.75

Figure Lengend Snippet: ASPC-1 or BxPC3 cells were injected subcutaneously in athymic nude mice. After tumors reached a volume of 30–40 mm 3 , they were either injected with buffer (ASPC-1) or with luciferase-directed (sh-Luc) or cyclin D1-directed (sh-D1) shRNA-lentiviruses. Tumor volumes were calculated in mm 3 as described in the Methods section. Data are expressed as means ± SEM from 8 mice in each group at each time point. *p<0.05 when compared with respective controls.

Article Snippet: Briefly, PVDF membranes were incubated overnight with a 1:1000 dilution of anti-cyclin D1 human anti-mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA), washed, and incubated for 30 minutes with a secondary horseradish peroxidase-conjugated antibody.

Techniques: Injection, Luciferase, shRNA

Journal: Cancer gene therapy

Article Title: Intra-Tumoral Delivery of shRNA Targeting Cyclin D1 Attenuates Pancreatic Cancer Growth

doi: 10.1038/cgt.2009.75

Figure Lengend Snippet: (a) Immunohistochemical analysis. Representative immunostaining for Ki-67, CD31 and VEGF in ASPC-1-derived tumors that were injected in vivo with buffer (Control), a luciferase-directed shRNA-lentivirus (sh-Luc), or a cyclin D1-directed shRNA-lentivirus (sh-D1). Original magnification, x 20. (b) Quantitative morphometry for Ki-67, CD31, and VEGF immunoreactivity from the tumors shown in (a). Data are the means ± SEM from three tumors/group, with 10 random fields analyzed for each tumor. * P <0.05, **p<0.01 when compared with respective controls.

Article Snippet: Briefly, PVDF membranes were incubated overnight with a 1:1000 dilution of anti-cyclin D1 human anti-mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA), washed, and incubated for 30 minutes with a secondary horseradish peroxidase-conjugated antibody.

Techniques: Immunohistochemical staining, Immunostaining, Derivative Assay, Injection, In Vivo, Luciferase, shRNA

Journal: Cancer gene therapy

Article Title: Intra-Tumoral Delivery of shRNA Targeting Cyclin D1 Attenuates Pancreatic Cancer Growth

doi: 10.1038/cgt.2009.75

Figure Lengend Snippet: (a) Immunohistochemical analysis. Representative immunostaining for Ki-67, CD31 and VEGF in tumors BxPC3-derived tumors that were injected in vivo with buffer (Control), a luciferase-directed shRNA-lentivirus (sh-Luc), or a cyclin D1-directed shRNA-lentivirus (sh-D1). Original magnification, x 20. (b) Quantitative morphometry for Ki-67, CD31, and VEGF immunoreactivity. Data are the means ± SEM from three tumors/cell line/group. * P <0.05, **p<0.01 when compared with respective controls.

Article Snippet: Briefly, PVDF membranes were incubated overnight with a 1:1000 dilution of anti-cyclin D1 human anti-mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA), washed, and incubated for 30 minutes with a secondary horseradish peroxidase-conjugated antibody.

Techniques: Immunohistochemical staining, Immunostaining, Derivative Assay, Injection, In Vivo, Luciferase, shRNA