Review



mouse anti human c5b 9 fitc  (Hycult Biotech)


Bioz Verified Symbol Hycult Biotech is a verified supplier
Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 92
      Buy from Supplier

    Structured Review

    Hycult Biotech mouse anti human c5b 9 fitc
    ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement <t>C5b-9</t> and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.
    Mouse Anti Human C5b 9 Fitc, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human c5b 9 fitc/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    mouse anti human c5b 9 fitc - by Bioz Stars, 2025-07
    92/100 stars

    Images

    1) Product Images from "Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood"

    Article Title: Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-04898-w

    ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement C5b-9 and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.
    Figure Legend Snippet: ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement C5b-9 and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.

    Techniques Used: Activation Assay, Coagulation, Enzyme-linked Immunosorbent Assay, Expressing, Concentration Assay, Incubation



    Similar Products

    mouse anti human c5b 9 fitc  (Hycult Biotech)
    92
    Hycult Biotech mouse anti human c5b 9 fitc
    ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement <t>C5b-9</t> and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.
    Mouse Anti Human C5b 9 Fitc, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human c5b 9 fitc/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    mouse anti human c5b 9 fitc - by Bioz Stars, 2025-07
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement C5b-9 and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.

    Journal: Scientific Reports

    Article Title: Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood

    doi: 10.1038/s41598-017-04898-w

    Figure Lengend Snippet: ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement C5b-9 and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.

    Article Snippet: Rabbit anti-human C3b/c FITC (A0062; Dako), mouse anti-human C5b-9 FITC (clone aE11; HM2167F, Hycult Biotech), and mouse anti-human CD62E FITC (clone 1.2B6, MCA883F, AbD Serotec) were diluted in PBS/1% BSA and incubated for 60 min at RT followed by three washes.

    Techniques: Activation Assay, Coagulation, Enzyme-linked Immunosorbent Assay, Expressing, Concentration Assay, Incubation