mouse anti human α sma monoclonal antibody (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc mouse anti human α sma monoclonal antibody

The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) <t>α</t> <t>-SMA</t> level in LX-2. The levels of <t>α</t> <t>-SMA</t> protein were determined by Western blot using anti- <t>α</t> <t>-SMA</t> antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

Mouse Anti Human α Sma Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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mouse anti human α sma monoclonal antibody - by Bioz Stars, 2023-03

86/100 stars

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1) Product Images from "Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways"

Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2012/960128

Figure Legend Snippet: The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

Techniques Used: Inhibition, Western Blot, Negative Control

The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

Figure Legend Snippet: The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

Techniques Used: Inhibition, Western Blot, Negative Control

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mouse anti human α sma monoclonal antibody (Cell Signaling Technology Inc)

Cell Signaling Technology Inc mouse anti human α sma monoclonal antibody

mouse anti human α sma monoclonal antibody - by Bioz Stars, 2023-03

86/100 stars

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Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

doi: 10.1155/2012/960128

Figure Lengend Snippet: The effects of SA-B on p38 MAPK pathway via Inhibition of ERK and Smad signaling. (a) P38 phosphorylation in LX-2 cells. The levels of phosphorylated p38 protein were determined by Western blot using anti-phospho-p38 antibodies. The levels of total p38 protein were determined by Western blot using anti-p38 antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ■■■ Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ▲▲▲ Significant difference versus TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + PD ( n = 3, P < 0.001). (b) α -SMA level in LX-2. The levels of α -SMA protein were determined by Western blot using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.001), ▵▵▵ Significant difference SM4 + TGF + PD and SM4 + TGF + SA-B ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SA-B, TGF + SA-B + PD ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ▵▵ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); *Significant difference versus SM4 + TGF + PD, SM4 + TGF + SA-B, and TGF + SA-B + PD ( n = 3, P < 0.05).

Article Snippet: Smad4 polyclonal antibody, p-p38 monoclonal antibody, p38, p-MEK and MEK monoclonal antibody were purchased from Cell Signaling Technology (Beverly, MA, USA); mouse anti-human α -SMA monoclonal antibody and Trizol reagent were purchased from Sigma (St Louis, MO, USA).

Techniques: Inhibition, Western Blot, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF- β 1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

doi: 10.1155/2012/960128

Figure Lengend Snippet: The effects of SA-B on ERK signaling via inhibition of p38 MAPK and Smad signaling. (a) MEK phosphorylation in LX-2 cells. The levels of phosphorylated MEK protein were determined by Western blot using anti-phospho-MEK antibodies. The levels of total MEK protein were determined by Western blot using anti-MEK antibodies. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). ### Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.001); **Significant difference versus TGF ( n = 3, P < 0.01); ▲▲▲ Significant difference versus SM4 + TGF, SM4 + TGF + SB, and TGF + SA-B + SB ( n = 3, P < 0.01). (b) α -SMA levels in LX-2. The levels of α -SMA protein were determined using anti- α -SMA antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ⋄⋄ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); Significant difference versus SM4 + TGF ( n = 3, P < 0.001); ***Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.001). (c) Col. I level in LX-2. The levels of Col. I protein were determined by Western blot using anti-Col. I antibodies. Blotting with anti-GAPDH antibodies was conducted as a protein loading control. ## Significant difference versus Control, Negative control, and SRV4 ( n = 3, P < 0.01); ▲▲ Significant difference versus TGF ( n = 3, P < 0.01); ◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.05); ◊◊ Significant difference versus SM4 + TGF ( n = 3, P < 0.01); **Significant difference versus SM4 + TGF + SB, SM4 + TGF + SA-B, and TGF + SA-B + SB ( n = 3, P < 0.01).

Techniques: Inhibition, Western Blot, Negative Control