Journal: BMC Neuroscience

Article Title: Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates

doi: 10.1186/1471-2202-11-7

Figure Lengend Snippet: Adaptation of culture conditions of aNS-1 cells to microplates . aNS-1 cells can be cultured in 96- ( A ) and 384-well ( B ) plates, maintaining the expression of proper NS cells markers (Nestin, Vimentin, BLBP, Olig2), without exiting the cell cycle (Phospho-Histone3 immunoreactivity, P-H3), without differentiation (beta III-tubulin, MAP2 and GFAP absence) or cell death (cleaved caspase-3 absence).

Article Snippet: Primary antibodies used in this work are: anti beta III-tubulin (1:1000; Promega); anti BLBP (1:1000; Chemicon); anti cleaved caspase-3 (1:100; Cell Signaling); anti GFAP (1:750; DAKO); anti MAP2 (1:500; Chemicon); anti Nestin (1:250; Chemicon); anti Olig2 (1:500; Chemicon); anti Phospho Histone3 (1:100; Cell Signaling); anti Vimentin (1:100; Developmental Studies Hybridoma Bank).

Techniques: Cell Culture, Expressing