gfp mouse mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gfp mouse mab
    Gfp Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti gfp mouse monoclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti gfp mouse monoclonal antibody
    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    1) Product Images from "Tissue-specific O- GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning"

    Article Title: Tissue-specific O- GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning

    Journal: eLife

    doi: 10.7554/eLife.91269

    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, and anti-GFP (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
    Figure Legend Snippet: ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, and anti-GFP (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .

    Techniques Used: Expressing, Immunostaining, Staining

    ( A ) Heatmaps showing the mRNA levels (upper) and the normalized O- GlcNAc levels (lower) of the identified ribosomal candidates in different brain regions. ( B ) Immunoprecipitation of ribosomes using FLAG-tagged RpL13A. The expression of RpL13A-FLAG was validated by immunoblotting with anti-FLAG antibody. Ribosomal proteins were visualized using silver staining, and O- GlcNAcylation of ribosomes was analyzed by immunoblotting with anti- O- GlcNAc antibody RL2. ( C ) A compilation of the performance index of the indicated flies in the learning test. Learning defect of flies expressing Cp OGA WT was corrected by selective expression of dMyc in mushroom body. n = 6-7. Each datapoint represents an independent experiment with approximately 200 flies. ( D ) Ex vivo measurement of protein synthesis in mushroom body using the O-propargyl-puromycin (OPP) assay. Brains from the indicated flies were stained with anti-GFP (green) antibody to validate Cp OGA expression, and OPP (gray) to quantify protein synthesis. Nuclei were visualized with DAPI (blue). Outlined areas indicate the cell bodies of Kenyon cells of mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative OPP fluorescent intensity in mushroom body regions. n = 8-12. p -values were determined by unpaired t -test, the stars indicate significant differences (***p<0.001, **p<0.01, *p<0.05). Error bars represent SD. Figure 4—source data 1. Raw data of all western blots for . Figure 4—source data 2. Complete and uncropped membranes of all western blots for . Figure 4—source data 3. Excel spreadsheet containing source data used to generate .
    Figure Legend Snippet: ( A ) Heatmaps showing the mRNA levels (upper) and the normalized O- GlcNAc levels (lower) of the identified ribosomal candidates in different brain regions. ( B ) Immunoprecipitation of ribosomes using FLAG-tagged RpL13A. The expression of RpL13A-FLAG was validated by immunoblotting with anti-FLAG antibody. Ribosomal proteins were visualized using silver staining, and O- GlcNAcylation of ribosomes was analyzed by immunoblotting with anti- O- GlcNAc antibody RL2. ( C ) A compilation of the performance index of the indicated flies in the learning test. Learning defect of flies expressing Cp OGA WT was corrected by selective expression of dMyc in mushroom body. n = 6-7. Each datapoint represents an independent experiment with approximately 200 flies. ( D ) Ex vivo measurement of protein synthesis in mushroom body using the O-propargyl-puromycin (OPP) assay. Brains from the indicated flies were stained with anti-GFP (green) antibody to validate Cp OGA expression, and OPP (gray) to quantify protein synthesis. Nuclei were visualized with DAPI (blue). Outlined areas indicate the cell bodies of Kenyon cells of mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative OPP fluorescent intensity in mushroom body regions. n = 8-12. p -values were determined by unpaired t -test, the stars indicate significant differences (***p<0.001, **p<0.01, *p<0.05). Error bars represent SD. Figure 4—source data 1. Raw data of all western blots for . Figure 4—source data 2. Complete and uncropped membranes of all western blots for . Figure 4—source data 3. Excel spreadsheet containing source data used to generate .

    Techniques Used: Immunoprecipitation, Expressing, Western Blot, Silver Staining, Ex Vivo, Staining


    Figure Legend Snippet:

    Techniques Used: Silver Staining, Protease Inhibitor, Magnetic Beads, SYBR Green Assay, Sequencing, Modification, Software, Microscopy

    primary mouse anti gfp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary mouse anti gfp
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    gfp 4b10 mouse mab  (Cell Signaling Technology Inc)


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    mouse anti gfp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti gfp
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    gfp mouse mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc gfp mouse mab
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    anti gfp mouse  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti gfp mouse
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    anti gfp mouse  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti gfp mouse
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    antibodies mouse anti gfp living color  (Cell Signaling Technology Inc)


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    gfp 4b10 mouse mab  (Cell Signaling Technology Inc)


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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, <t>and</t> <t>anti-GFP</t> (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .
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    ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, and anti-GFP (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .

    Journal: eLife

    Article Title: Tissue-specific O- GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning

    doi: 10.7554/eLife.91269

    Figure Lengend Snippet: ( A ) Scheme for expression of Cp OGA WT or Cp OGA DM in various Drosophila brain structures using different Gal4 drivers. ( B ) Immunostaining of adult Drosophila brains. Brains were stained with anti- O- GlcNAc antibody RL2 (red) to assess O- GlcNAcylation level, and anti-GFP (green) antibody to validate tissue-specific expression of Cp OGA. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. ( C ) Quantification of fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brains. n = 4. ( D ) Immunostaining of adult Drosophila brains. Outlined areas indicate the cell bodies of Kenyon cells in mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative fluorescent intensity of O- GlcNAc staining in Cp OGA WT or Cp OGA DM expressed brain structures. n = 8. ( F ) A compilation of performance index in learning test of the indicated flies expressing either Cp OGA WT or Cp OGA DM . n = 6-8. ( G ) A compilation of learning performance index of flies expressing Cp OGA WT or Cp OGA DM only in the mushroom body at adult stage. n = 6. Each datapoint represents an independent experiment with approximately 200 flies. p- values were determined by unpaired t -test, and the stars indicate significant differences (***p<0.001, **p<0.01 and ns, not significant, p≥0.05). Error bars represent SD. Figure 1—source data 1. Excel spreadsheet containing source data used to generate .

    Article Snippet: Antibody , Anti-GFP Mouse Monoclonal Antibody (4B10) , Cell Signaling Technology , Cat# 2955, RRID: AB_1196614 , WB (1:1000) IF (1:200).

    Techniques: Expressing, Immunostaining, Staining

    ( A ) Heatmaps showing the mRNA levels (upper) and the normalized O- GlcNAc levels (lower) of the identified ribosomal candidates in different brain regions. ( B ) Immunoprecipitation of ribosomes using FLAG-tagged RpL13A. The expression of RpL13A-FLAG was validated by immunoblotting with anti-FLAG antibody. Ribosomal proteins were visualized using silver staining, and O- GlcNAcylation of ribosomes was analyzed by immunoblotting with anti- O- GlcNAc antibody RL2. ( C ) A compilation of the performance index of the indicated flies in the learning test. Learning defect of flies expressing Cp OGA WT was corrected by selective expression of dMyc in mushroom body. n = 6-7. Each datapoint represents an independent experiment with approximately 200 flies. ( D ) Ex vivo measurement of protein synthesis in mushroom body using the O-propargyl-puromycin (OPP) assay. Brains from the indicated flies were stained with anti-GFP (green) antibody to validate Cp OGA expression, and OPP (gray) to quantify protein synthesis. Nuclei were visualized with DAPI (blue). Outlined areas indicate the cell bodies of Kenyon cells of mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative OPP fluorescent intensity in mushroom body regions. n = 8-12. p -values were determined by unpaired t -test, the stars indicate significant differences (***p<0.001, **p<0.01, *p<0.05). Error bars represent SD. Figure 4—source data 1. Raw data of all western blots for . Figure 4—source data 2. Complete and uncropped membranes of all western blots for . Figure 4—source data 3. Excel spreadsheet containing source data used to generate .

    Journal: eLife

    Article Title: Tissue-specific O- GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning

    doi: 10.7554/eLife.91269

    Figure Lengend Snippet: ( A ) Heatmaps showing the mRNA levels (upper) and the normalized O- GlcNAc levels (lower) of the identified ribosomal candidates in different brain regions. ( B ) Immunoprecipitation of ribosomes using FLAG-tagged RpL13A. The expression of RpL13A-FLAG was validated by immunoblotting with anti-FLAG antibody. Ribosomal proteins were visualized using silver staining, and O- GlcNAcylation of ribosomes was analyzed by immunoblotting with anti- O- GlcNAc antibody RL2. ( C ) A compilation of the performance index of the indicated flies in the learning test. Learning defect of flies expressing Cp OGA WT was corrected by selective expression of dMyc in mushroom body. n = 6-7. Each datapoint represents an independent experiment with approximately 200 flies. ( D ) Ex vivo measurement of protein synthesis in mushroom body using the O-propargyl-puromycin (OPP) assay. Brains from the indicated flies were stained with anti-GFP (green) antibody to validate Cp OGA expression, and OPP (gray) to quantify protein synthesis. Nuclei were visualized with DAPI (blue). Outlined areas indicate the cell bodies of Kenyon cells of mushroom body. Scale bar: 100 μm. ( E ) Quantification of relative OPP fluorescent intensity in mushroom body regions. n = 8-12. p -values were determined by unpaired t -test, the stars indicate significant differences (***p<0.001, **p<0.01, *p<0.05). Error bars represent SD. Figure 4—source data 1. Raw data of all western blots for . Figure 4—source data 2. Complete and uncropped membranes of all western blots for . Figure 4—source data 3. Excel spreadsheet containing source data used to generate .

    Article Snippet: Antibody , Anti-GFP Mouse Monoclonal Antibody (4B10) , Cell Signaling Technology , Cat# 2955, RRID: AB_1196614 , WB (1:1000) IF (1:200).

    Techniques: Immunoprecipitation, Expressing, Western Blot, Silver Staining, Ex Vivo, Staining

    Journal: eLife

    Article Title: Tissue-specific O- GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning

    doi: 10.7554/eLife.91269

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-GFP Mouse Monoclonal Antibody (4B10) , Cell Signaling Technology , Cat# 2955, RRID: AB_1196614 , WB (1:1000) IF (1:200).

    Techniques: Silver Staining, Protease Inhibitor, Magnetic Beads, SYBR Green Assay, Sequencing, Modification, Software, Microscopy