Structured Review

Millipore mouse anti flag m2 monoclonal antibody
Mouse Anti Flag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag m2 monoclonal antibody - by Bioz Stars, 2024-05
86/100 stars

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Millipore mouse anti flag tag m2 monoclonal antibody
Mouse Anti Flag Tag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag tag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag tag m2 monoclonal antibody - by Bioz Stars, 2024-05
86/100 stars

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Structured Review

Millipore mouse anti flag m2 monoclonal antibody
Mouse Anti Flag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag m2 monoclonal antibody - by Bioz Stars, 2024-05
86/100 stars

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Structured Review

Millipore mouse anti flag tag m2 monoclonal antibody
Mouse Anti Flag Tag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti flag tag m2 monoclonal antibody/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti flag tag m2 monoclonal antibody - by Bioz Stars, 2024-05
86/100 stars

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Structured Review

Millipore anti flag m2 mouse monoclonal
Anti Flag M2 Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti flag m2 mouse monoclonal/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti flag m2 mouse monoclonal - by Bioz Stars, 2024-05
86/100 stars

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mouse m2 monoclonal anti flag primary antibody  (Millipore)

 
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    Structured Review

    Millipore mouse m2 monoclonal anti flag primary antibody
    NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express <t>FLAG-tagged</t> NKT secrete NKT (∼19 kDa) in the medium, as shown using <t>anti-FLAG</t> immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.
    Mouse M2 Monoclonal Anti Flag Primary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse m2 monoclonal anti flag primary antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse m2 monoclonal anti flag primary antibody - by Bioz Stars, 2024-05
    86/100 stars

    Images

    1) Product Images from "Drosophila noktochor regulates night sleep via a local mushroom body circuit"

    Article Title: Drosophila noktochor regulates night sleep via a local mushroom body circuit

    Journal: iScience

    doi: 10.1016/j.isci.2024.109106

    NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express FLAG-tagged NKT secrete NKT (∼19 kDa) in the medium, as shown using anti-FLAG immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.
    Figure Legend Snippet: NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express FLAG-tagged NKT secrete NKT (∼19 kDa) in the medium, as shown using anti-FLAG immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.

    Techniques Used: In Vitro, Cell Culture, In Vivo, Recombinant, Immunoprecipitation, Plasmid Preparation, Positive Control, Transgenic Assay, Fluorescence, Expressing


    Figure Legend Snippet:

    Techniques Used: Recombinant, Generated, Expressing, Mutagenesis


    Structured Review

    Millipore anti flag m2 mouse monoclonal
    Lytic reactivation after depletion of LANA. ( A ) Immunofluorescence microscopy. iSLK-OsTIR1-mAID-LANA BAC16 cells and iSLK-OsTIR1-BAC16 WT cells were treated with or without 2 μM 5-Ph-IAA (± IAA) for 24 h, treated with or without 1-μg/mL doxycycline plus 1.5-mM sodium butyrate (±D/N) for another 24 h, and then subjected to immunofluorescence staining with <t>anti-FLAG,</t> LANA, and K8α antibodies. Bar, 100 μm. ( B ) Western blotting. iSLK-OsTIR1-mAID-LANA BAC16 cells and iSLK-OsTIR1-BAC16 WT cells were treated with 2-μM 5-Ph-IAA (IAA) for 0, 1.5, 3.0, 6.0, and 24.0 h, and then treated with or without 1-μg/mL doxycycline plus 1.5-mM sodium butyrate (±D/N) for an additional 24 h. The total cell lysate was prepared and subjected to immunoblotting with indicated antibodies. ( C ) Real-time qPCR. Total RNA was extracted and subjected to real-time qPCR. 18S rRNA was used as an internal standard for normalization, and the IAA (−) D/N (−) was set as 1.
    Anti Flag M2 Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag m2 mouse monoclonal/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti flag m2 mouse monoclonal - by Bioz Stars, 2024-05
    86/100 stars

    Images

    1) Product Images from "Kaposi’s sarcoma-associated herpesvirus (KSHV) LANA prevents KSHV episomes from degradation"

    Article Title: Kaposi’s sarcoma-associated herpesvirus (KSHV) LANA prevents KSHV episomes from degradation

    Journal: Journal of Virology

    doi: 10.1128/jvi.01268-23

    Lytic reactivation after depletion of LANA. ( A ) Immunofluorescence microscopy. iSLK-OsTIR1-mAID-LANA BAC16 cells and iSLK-OsTIR1-BAC16 WT cells were treated with or without 2 μM 5-Ph-IAA (± IAA) for 24 h, treated with or without 1-μg/mL doxycycline plus 1.5-mM sodium butyrate (±D/N) for another 24 h, and then subjected to immunofluorescence staining with anti-FLAG, LANA, and K8α antibodies. Bar, 100 μm. ( B ) Western blotting. iSLK-OsTIR1-mAID-LANA BAC16 cells and iSLK-OsTIR1-BAC16 WT cells were treated with 2-μM 5-Ph-IAA (IAA) for 0, 1.5, 3.0, 6.0, and 24.0 h, and then treated with or without 1-μg/mL doxycycline plus 1.5-mM sodium butyrate (±D/N) for an additional 24 h. The total cell lysate was prepared and subjected to immunoblotting with indicated antibodies. ( C ) Real-time qPCR. Total RNA was extracted and subjected to real-time qPCR. 18S rRNA was used as an internal standard for normalization, and the IAA (−) D/N (−) was set as 1.
    Figure Legend Snippet: Lytic reactivation after depletion of LANA. ( A ) Immunofluorescence microscopy. iSLK-OsTIR1-mAID-LANA BAC16 cells and iSLK-OsTIR1-BAC16 WT cells were treated with or without 2 μM 5-Ph-IAA (± IAA) for 24 h, treated with or without 1-μg/mL doxycycline plus 1.5-mM sodium butyrate (±D/N) for another 24 h, and then subjected to immunofluorescence staining with anti-FLAG, LANA, and K8α antibodies. Bar, 100 μm. ( B ) Western blotting. iSLK-OsTIR1-mAID-LANA BAC16 cells and iSLK-OsTIR1-BAC16 WT cells were treated with 2-μM 5-Ph-IAA (IAA) for 0, 1.5, 3.0, 6.0, and 24.0 h, and then treated with or without 1-μg/mL doxycycline plus 1.5-mM sodium butyrate (±D/N) for an additional 24 h. The total cell lysate was prepared and subjected to immunoblotting with indicated antibodies. ( C ) Real-time qPCR. Total RNA was extracted and subjected to real-time qPCR. 18S rRNA was used as an internal standard for normalization, and the IAA (−) D/N (−) was set as 1.

    Techniques Used: Immunofluorescence, Microscopy, Staining, Western Blot


    Structured Review

    Millipore mouse monoclonal anti flag m2
    Mouse Monoclonal Anti Flag M2, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti flag m2/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti flag m2 - by Bioz Stars, 2024-05
    86/100 stars

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    Structured Review

    Millipore mouse monoclonal anti flag m2 antibody
    Mouse Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti flag m2 antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti flag m2 antibody - by Bioz Stars, 2024-05
    86/100 stars

    Images


    Structured Review

    Millipore mouse monoclonal anti flag m2
    Mouse Monoclonal Anti Flag M2, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti flag m2/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti flag m2 - by Bioz Stars, 2024-05
    86/100 stars

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    Millipore mouse anti flag m2 monoclonal antibody
    Mouse Anti Flag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti flag m2 monoclonal antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti flag m2 monoclonal antibody - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Millipore mouse anti flag tag m2 monoclonal antibody
    Mouse Anti Flag Tag M2 Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti flag tag m2 monoclonal antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti flag tag m2 monoclonal antibody - by Bioz Stars, 2024-05
    86/100 stars
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    86
    Millipore anti flag m2 mouse monoclonal
    Anti Flag M2 Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag m2 mouse monoclonal/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti flag m2 mouse monoclonal - by Bioz Stars, 2024-05
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      Buy from Supplier

    86
    Millipore mouse m2 monoclonal anti flag primary antibody
    NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express <t>FLAG-tagged</t> NKT secrete NKT (∼19 kDa) in the medium, as shown using <t>anti-FLAG</t> immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.
    Mouse M2 Monoclonal Anti Flag Primary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse m2 monoclonal anti flag primary antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse m2 monoclonal anti flag primary antibody - by Bioz Stars, 2024-05
    86/100 stars
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    86
    Millipore mouse monoclonal anti flag m2
    NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express <t>FLAG-tagged</t> NKT secrete NKT (∼19 kDa) in the medium, as shown using <t>anti-FLAG</t> immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.
    Mouse Monoclonal Anti Flag M2, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti flag m2/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti flag m2 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Millipore mouse monoclonal anti flag m2 antibody
    NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express <t>FLAG-tagged</t> NKT secrete NKT (∼19 kDa) in the medium, as shown using <t>anti-FLAG</t> immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.
    Mouse Monoclonal Anti Flag M2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti flag m2 antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti flag m2 antibody - by Bioz Stars, 2024-05
    86/100 stars
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    NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express FLAG-tagged NKT secrete NKT (∼19 kDa) in the medium, as shown using anti-FLAG immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.

    Journal: iScience

    Article Title: Drosophila noktochor regulates night sleep via a local mushroom body circuit

    doi: 10.1016/j.isci.2024.109106

    Figure Lengend Snippet: NKT is secreted in vitro from cultured cells and in vivo from mushroom body α′/β′ neurons (A) Recombinant HEK293 cells that express FLAG-tagged NKT secrete NKT (∼19 kDa) in the medium, as shown using anti-FLAG immunoprecipitation (lane 1). Residual tagged-NKT is detected in the cell lysate (lane 2), but none is present in the medium from cultured cells that do not express the NKT-FLAG plasmid (PCD media, lane 3). As expected, an anti-FLAG signal is present in positive control lane 4 that was loaded with FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP, indicated by ∗). (B) RB35B12-Gal4>UAS-NKT-GFP double transgenic flies show expected fluorescence in MB neurons (upper left panel). Decreased NKT-GFP fluorescence, an index of protein secretion, was observed following KCl-induced neuron depolarization (bottom left panel). No significant difference was observed between KCl-treated and non-treated brains that expressed soluble GFP alone (right panels). (C and D) Quantification of corresponding GFP pixel signal intensity observed in treated, vs. non-treated brains. Student’s t test was utilized to compare the “No KCl” and “KCl” treatment groups for GFP and NKT-GFP expression. ns, not significant, ∗∗, p < 0.01.

    Article Snippet: Mouse M2 monoclonal anti-FLAG primary antibody purchased from Millipore Sigma was added to 5% milk in TBST for a final dilution of 1:250 and incubated overnight.

    Techniques: In Vitro, Cell Culture, In Vivo, Recombinant, Immunoprecipitation, Plasmid Preparation, Positive Control, Transgenic Assay, Fluorescence, Expressing

    Journal: iScience

    Article Title: Drosophila noktochor regulates night sleep via a local mushroom body circuit

    doi: 10.1016/j.isci.2024.109106

    Figure Lengend Snippet:

    Article Snippet: Mouse M2 monoclonal anti-FLAG primary antibody purchased from Millipore Sigma was added to 5% milk in TBST for a final dilution of 1:250 and incubated overnight.

    Techniques: Recombinant, Generated, Expressing, Mutagenesis