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Santa Cruz Biotechnology mouse anti caga
Mouse Anti Caga, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti caga/product/Santa Cruz Biotechnology
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mouse anti caga - by Bioz Stars, 2020-09
93/100 stars

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Article Snippet: Mouse anti-CagA, and anti-IgG were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

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    Santa Cruz Biotechnology mouse anti caga monoclonal antibody
    Effect of anthocyanins on secretion of H. pylori <t>CagA</t> and <t>VacA.</t> H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days and proteins assessed by Western blot. (A), secreted CagA and VacA in culture media. (B), secreted H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. (C) , intracellular CagA and VacA. (D) , intracellular H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. Representative image from five independent experiments.
    Mouse Anti Caga Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti caga monoclonal antibody/product/Santa Cruz Biotechnology
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mouse anti caga monoclonal antibody - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology rabbit anti caga
    Surface localization of <t>CagA</t> in wild-type and HpΔcagI strains. ( A ) Immunofluorescence microscopy showing surface localization of CagA in wild-type and HpΔcagI strains . Wild-type and HpΔcagI cells were fixed and one set was permeabilized with 0.2% Triton X-100. M, NP and P stand for merge, non-permeabilized and permeabilized cell respectively. Primary antibodies used in IFM are indicated. Secondary antibodies used were Alexa fluor 488 (green colour) and Alexa fluor 594 (red colour) conjugated. ( B and C ) Immunogold electron microscopy (IEM) showing surface localization of CagA in Hp Δ <t>cagI</t> and wild-type H. pylori (Hp) strains . Immunogold labeling of H. pylori strains ultrathin sections were performed as described in Materials and Methods. ( B ) HpΔcagI cells were stained with anti-CagA and gold-labeled secondary antibody. ( C ) Wild-type H. pylori cells were stained with anti-CagA and gold-labeled secondary antibody. ( D ) HpΔcagA cells were stained with anti-CagA and gold-labeled secondary antibody. ( E ) Pre-immune (preimm.) serum was used as negative control. Scale bars indicate 100 nm. Arrowheads indicate location of gold-labeled secondary antibody.
    Rabbit Anti Caga, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti caga/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti caga - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Effect of anthocyanins on secretion of H. pylori CagA and VacA. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days and proteins assessed by Western blot. (A), secreted CagA and VacA in culture media. (B), secreted H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. (C) , intracellular CagA and VacA. (D) , intracellular H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. Representative image from five independent experiments.

    Journal: International Journal of Medical Sciences

    Article Title: Inhibitory Effects of Anthocyanins on Secretion of Helicobacter pylori CagA and VacA Toxins

    doi: 10.7150/ijms.5094

    Figure Lengend Snippet: Effect of anthocyanins on secretion of H. pylori CagA and VacA. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days and proteins assessed by Western blot. (A), secreted CagA and VacA in culture media. (B), secreted H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. (C) , intracellular CagA and VacA. (D) , intracellular H. pylori proteins reacting with rabbit anti- H. pylori polyclonal antibody. Representative image from five independent experiments.

    Article Snippet: Membranes were blocked with 5% skim milk for 30 minutes and then incubated with mouse anti-CagA monoclonal antibody (Santa Cruz Biotechnology, CA, USA), rabbit anti-VacA polyclonal antibody (Santa Cruz Biotechnology) or rabbit anti-Helicobacter pylori polyclonal antibody (this study).

    Techniques: Cell Culture, Western Blot

    Ubiquitination of total proteins and proteasome activity in AGS cells co-cultured with H. pylori . (A) Representative immunoblot of total ubiquitinated protein from AGS cells infected with H. pylori (MOI = 300) for 8 h, in presence or absence of 2.5 μM Lactacystin. Tubulin is shown as loading control and CagA as infection control. (B) Quantification of proteasome activity in AGS cells infected with H. pylori wild type and its isogenic mutants cagA, vacA and, in presence of 2.5 μM Lactacystin for 8 h. Asterisk represents significant difference ( p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence

    doi: 10.3389/fcimb.2017.00450

    Figure Lengend Snippet: Ubiquitination of total proteins and proteasome activity in AGS cells co-cultured with H. pylori . (A) Representative immunoblot of total ubiquitinated protein from AGS cells infected with H. pylori (MOI = 300) for 8 h, in presence or absence of 2.5 μM Lactacystin. Tubulin is shown as loading control and CagA as infection control. (B) Quantification of proteasome activity in AGS cells infected with H. pylori wild type and its isogenic mutants cagA, vacA and, in presence of 2.5 μM Lactacystin for 8 h. Asterisk represents significant difference ( p

    Article Snippet: Anti-Tubulin mouse monoclonal (Catalog number: T5168, Sigma-Aldrich, St Louis, Missouri, USA) and anti-CagA mouse monoclonal antibodies (Catalog number: sc-28368, Santa Cruz Biotechnology, Dallas, Texas, USA) were used as loading and H. pylori -infection controls, respectively.

    Techniques: Activity Assay, Cell Culture, Infection

    Surface localization of CagA in wild-type and HpΔcagI strains. ( A ) Immunofluorescence microscopy showing surface localization of CagA in wild-type and HpΔcagI strains . Wild-type and HpΔcagI cells were fixed and one set was permeabilized with 0.2% Triton X-100. M, NP and P stand for merge, non-permeabilized and permeabilized cell respectively. Primary antibodies used in IFM are indicated. Secondary antibodies used were Alexa fluor 488 (green colour) and Alexa fluor 594 (red colour) conjugated. ( B and C ) Immunogold electron microscopy (IEM) showing surface localization of CagA in Hp Δ cagI and wild-type H. pylori (Hp) strains . Immunogold labeling of H. pylori strains ultrathin sections were performed as described in Materials and Methods. ( B ) HpΔcagI cells were stained with anti-CagA and gold-labeled secondary antibody. ( C ) Wild-type H. pylori cells were stained with anti-CagA and gold-labeled secondary antibody. ( D ) HpΔcagA cells were stained with anti-CagA and gold-labeled secondary antibody. ( E ) Pre-immune (preimm.) serum was used as negative control. Scale bars indicate 100 nm. Arrowheads indicate location of gold-labeled secondary antibody.

    Journal: PLoS ONE

    Article Title: Cag Type IV Secretion System: CagI Independent Bacterial Surface Localization of CagA

    doi: 10.1371/journal.pone.0074620

    Figure Lengend Snippet: Surface localization of CagA in wild-type and HpΔcagI strains. ( A ) Immunofluorescence microscopy showing surface localization of CagA in wild-type and HpΔcagI strains . Wild-type and HpΔcagI cells were fixed and one set was permeabilized with 0.2% Triton X-100. M, NP and P stand for merge, non-permeabilized and permeabilized cell respectively. Primary antibodies used in IFM are indicated. Secondary antibodies used were Alexa fluor 488 (green colour) and Alexa fluor 594 (red colour) conjugated. ( B and C ) Immunogold electron microscopy (IEM) showing surface localization of CagA in Hp Δ cagI and wild-type H. pylori (Hp) strains . Immunogold labeling of H. pylori strains ultrathin sections were performed as described in Materials and Methods. ( B ) HpΔcagI cells were stained with anti-CagA and gold-labeled secondary antibody. ( C ) Wild-type H. pylori cells were stained with anti-CagA and gold-labeled secondary antibody. ( D ) HpΔcagA cells were stained with anti-CagA and gold-labeled secondary antibody. ( E ) Pre-immune (preimm.) serum was used as negative control. Scale bars indicate 100 nm. Arrowheads indicate location of gold-labeled secondary antibody.

    Article Snippet: Rabbit anti-CagI (α-CagI), mice anti-CagH (α-CagH) and rabbit anti-CagA (Santa Cruz, USA) antibodies were used at a 1:10,000, 1:4000, 1:10,000 dilutions respectively.

    Techniques: Immunofluorescence, Microscopy, Electron Microscopy, Labeling, Staining, Negative Control