Journal: Frontiers in Cellular and Infection Microbiology
Article Title: KCTD5 and Ubiquitin Proteasome Signaling Are Required for Helicobacter pylori Adherence
Figure Lengend Snippet: Ubiquitination of total proteins and proteasome activity in AGS cells co-cultured with H. pylori . (A) Representative immunoblot of total ubiquitinated protein from AGS cells infected with H. pylori (MOI = 300) for 8 h, in presence or absence of 2.5 μM Lactacystin. Tubulin is shown as loading control and CagA as infection control. (B) Quantification of proteasome activity in AGS cells infected with H. pylori wild type and its isogenic mutants cagA, vacA and, in presence of 2.5 μM Lactacystin for 8 h. Asterisk represents significant difference ( p
Article Snippet: Anti-Tubulin mouse monoclonal (Catalog number: T5168, Sigma-Aldrich, St Louis, Missouri, USA) and anti-CagA mouse monoclonal antibodies (Catalog number: sc-28368, Santa Cruz Biotechnology, Dallas, Texas, USA) were used as loading and H. pylori -infection controls, respectively.
Techniques: Activity Assay, Cell Culture, Infection