mouse anti bax  (Boster Bio)

Journal: Molecular Medicine Reports

Article Title: Hippocampal mitogen-activated protein kinase activation is associated with intermittent hypoxia in a rat model of obstructive sleep apnea syndrome

doi: 10.3892/mmr.2015.4505

Figure Lengend Snippet: Bax-positive cells were detected using immunohistochemistry in the hippocampus after hypoxia (magnification, ×400). (A) Few Bax-positive cells in the control group. (B) Numerous Bax-positive cells with brown staining at the 6th week in continued hypoxia. (C) The number of Bax-positive cells was maximal at the 8th week in continued hypoxia; (D) Bax-positive cells at the 2nd week and (E) at the 8th week in the intermittent hypoxia group. Bax, Bcl-2-associated X protein.

Article Snippet: Furthermore, Bcl-2 rabbit polyclonal anti-mouse antibody and Bcl-2-associated X protein (Bax) polyclonal rabbit anti-mouse antibody were used (both from Wuhan Boster Biological Engineering Co., Ltd., Wuhan, China).

Techniques: Immunohistochemistry, Staining

Journal: Molecular Medicine Reports

Article Title: Hippocampal mitogen-activated protein kinase activation is associated with intermittent hypoxia in a rat model of obstructive sleep apnea syndrome

doi: 10.3892/mmr.2015.4505

Figure Lengend Snippet: Bcl-2-positive cells revealed by immunohistochemistry in the hippocampus following hypoxia (magnification, ×400). (A) Few Bcl-2-positive cells in the control group; (B) Numerous Bcl-2-positive cells with brown-staining at the 6th week, and (C) decreased number of positive cells at the 8th week in the continued hypoxia group; (D) Numerous Bcl-2 positive cells at the 2nd week and (E) decreased number of positive cells at the 6th week in the intermittent hypoxia group. Bcl-2, B cell lymphoma-2.

Article Snippet: Furthermore, Bcl-2 rabbit polyclonal anti-mouse antibody and Bcl-2-associated X protein (Bax) polyclonal rabbit anti-mouse antibody were used (both from Wuhan Boster Biological Engineering Co., Ltd., Wuhan, China).

Techniques: Immunohistochemistry, Staining

Journal: American Journal of Translational Research

Article Title: Inhibition of glioblastoma growth and invasion by 125I brachytherapy in rat glioma model

doi:

Figure Lengend Snippet: The implantation of 125 I seeds induced the mitochondria-dependent apoptotic pathway compared with the control. A: Western Blotting analysis of Bax, Bcl-2, pro-Caspase-3, Caspase-3, pro-Caspase-8, cleaved-Caspase-8, PARP, P53, β-Actin expression.

Article Snippet: After electrophoresis, proteins were electrotransferred to nitrocellulose membrane and immune complexes were formed by incubation of the proteins probed with primary antibodies, mouse anti-Bax, rabbit anti-Bcl-2, rabbit anti-Caspase-3, rabbit anti-Caspase-8 and rabbit anti-p53, rabbit anti-PARP, mouse anti-MMP-2, mouse anti-MMP-9 and mouse anti-Actin (Boster, Inc. China) for overnight at 4°C.

Techniques: Western Blot, Expressing

Journal: PLoS ONE

Article Title: Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function

doi: 10.1371/journal.pone.0052404

Figure Lengend Snippet: ScFvs against Fn1–3 protect cells from H 2 O 2 –induced death and increase the ratio of Bcl-2/Bax. SK-N-SH cells were seeded into poly-D-lysine (PDL)-pretreated culture plates coated with L1/ecd (as positive control), non-immune human IgG (as negative control), and scFvs I4, I6, I13, or I27 and maintained for 5 days in serum-free medium. In addition, SK-N-SH cells grown for 12 hours were pre-incubated with 250 µM H 2 O 2 for 2 hours in serum-free medium. After removal of H 2 O 2 , scFvs I13 and I27 were added at the indicated concentrations and cells were cultured for additional 24 hours. ( A ) ScFvs treatment for 5 days. Cell survival was measured by the MST-8 assay. ( B ) L1/ecd, non-immune human IgG, scFvs I4, I6, I13 or I27 (all at 16.5 µM) treatment for 24 hours for Western blot assay of Bcl-2 and Bax protein expression. ( C ) ScFv treatment for 12 hours. ( D ) ScFv treatment for 24 hours. ( C, D ) Cell viability was measured by the MST-8 assay. Data represent mean values ± SEM from four independent experiments. Asterisks denote significant differences from control. *** p

Article Snippet: Twenty five µg protein was subjected to 12% SDS-PAGE, transferred onto a PVDF membrane (Millipore, Temecula, CA, USA), and probed with rabbit anti-human src (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-phospho-src (Santa Cruz Biotechnology), mouse anti-phospho-Erk1/2 (Beyotime), rabbit anti-human Erk1/2 (Beyotime), rabbit anti-human Bcl-2 (Biosis, Bei Jing, China), mouse anti-human Bax, mouse anti-human GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (BOSTER, Wu Han, China).

Techniques: Positive Control, Negative Control, Incubation, Cell Culture, Microscale Thermophoresis, Western Blot, Expressing

Journal: Oncology Letters

Article Title: F10, a novel hydatidiform mole-associated gene, inhibits the paclitaxel sensitivity of A549 lung cancer cells by downregulating BAX and caspase-3

doi: 10.3892/ol.2017.5749

Figure Lengend Snippet: F10 reduced BAX and caspase-3 protein expression levels in paclitaxel-treated A549 cells. (A) The expression of BAX and caspase-3 was determined by a western blot analysis of paclitaxel-treated untransfected A549 cells, A549-mock cells or A549-F10 cells. The band signals of BAX and caspase-3 were normalized to that of β-actin. (B) The expression levels of BAX in paclitaxel-treated A549-F10 cells was lower compared with the untransfected A549 cells and A549-mock cells (*P

Article Snippet: The mouse monoclonal anti-human BAX (cat. no. BA0315) and anti-human β-actin (cat. no. BM0626) antibodies were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot