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Cell Signaling Technology Inc mouse anti actin ab
Mouse Anti Actin Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti actin ab/product/Cell Signaling Technology Inc
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
mouse anti actin ab - by Bioz Stars, 2020-09
88/100 stars

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Article Title: NF-κB-induced microRNA-31 promotes epidermal hyperplasia by repressing protein phosphatase 6 in psoriasis
Article Snippet: Mouse anti-actin Ab (1:3,000; Cell Signaling Technology, #3700S), rabbit anti-ppp6c Ab (1:1,000; Abcam, #EPR8764), rat anti-p65 Ab (Cell Signaling Technology, #8242S) and rabbit anti-mouse Ki67 Ab (eBioscience, #42–5698) were used.

Article Title: Activation of ERK and NF-κB during HARE-Mediated Heparin Uptake Require Only One of the Four Endocytic Motifs
Article Snippet: Rabbit anti-phospho-ERK1/2 (p44/42; Thr-P(202) and Tyr-P(204), anti-ERK1/2 and mouse anti-actin Abs were from Cell Signaling (Beverly, MA).

Article Title: A Hyaluronan Receptor for Endocytosis (HARE) Link Domain N-Glycan Is Required for Extracellular Signal-regulated Kinase (ERK) and Nuclear Factor-κB (NF-κB) Signaling in Response to the Uptake of Hyaluronan but Not Heparin, Dermatan Sulfate, or Acetylated Low Density Lipoprotein (LDL) *
Article Snippet: Rabbit anti-phospho-ERK1/2 (p44/42; Thr(P)202 and Tyr(P)204 ), anti-ERK1/2 and anti-IκB-α, and mouse anti-actin Abs were from Cell Signaling (Beverly, MA).

Article Title: Symptoms of systemic lupus erythematosus are diagnosed in leptin transgenic pigs
Article Snippet: Mouse anti-actin Ab (1:5,000; 3700S, Cell Signaling Technology), rabbit anti-p-stat3 Ab (1:1,000; sc-8001, Santa Cruz), rabbit anti-p-erk1/2 Ab (sc-13073, Santa Cruz), and rabbit anti-jak2 Ab (sc-278, Santa Cruz) were used.

Article Title: MicroRNA-31 negatively regulates peripherally derived regulatory T-cell generation by repressing retinoic acid-inducible protein 3
Article Snippet: Mouse anti-actin Ab (1:3,000) (Cell Signaling Technology), rabbit anti-Gprc5a Ab (1:1,000) (Dr Jiong Deng provided) were used.

Article Title: A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice
Article Snippet: Mouse anti-FLAG M2 antibody (1:2000 dilution, Sigma, Cat# F1804) and mouse anti-actin antibody (1:1,000 dilution, Cell Signaling Technology, Cat# 8457L) were used to detect Cas9 and actin.

Article Title: Inhibition of Influenza A Virus Replication by TRIM14 via Its Multifaceted Protein–Protein Interaction With NP
Article Snippet: Mouse anti-c-myc and mouse anti-beta actin antibody were from Cell Signaling Technology.

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    Cell Signaling Technology Inc monoclonal β actin
    Downregulation of p53 expression suppresses the pro-apoptotic effect of liriodenine. (A) Western blot analysis revealing the expression of p53 at different times following treatment with 10 μM liriodenine. <t>β-actin</t> was used as the internal control. Blots were repeated at least three times for statistical analysis. (B) Statistical analysis of p53 expression at different times following treatment with 10 μm liriodenine. ** P
    Monoclonal β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal β actin/product/Cell Signaling Technology Inc
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    monoclonal β actin - by Bioz Stars, 2020-09
    99/100 stars
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    88
    Cell Signaling Technology Inc mouse monoclonal anti serum against β actin
    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-9 (cleaved caspase-9) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase-9 (35 KDa) and <t>β-actin</t> as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P
    Mouse Monoclonal Anti Serum Against β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti serum against β actin/product/Cell Signaling Technology Inc
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti serum against β actin - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc rabbit anti β actin antibody
    A : anti-phosphorylated (p)TRPC6 (Thr 69 ) recognized pThr 70 in HEK-293 cells expressing hTRPC6 WT or hTRPC6 S322A but not cells expressing hTRPC6 T70A . B : anti-pTRPC6 (Thr 69 ) antibody detected pTRPC6 in the whole kidney and mouse primary podocyte lysates. Whole kidney protein extract ( left ) and primary podocyte cell lysates ( right ) from WT and TRPC6-deficient knockout (KO) mice were stained for pTRPC6, TRPC6, and <t>β-Actin.</t> C : time course of TRPC6 dephosphorylation in response to chronic ANG II stimulation. ANG II dephosphorylation occurred within 6 h of the initiation of ANG II treatment.
    Rabbit Anti β Actin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti β actin antibody/product/Cell Signaling Technology Inc
    Average 93 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    rabbit anti β actin antibody - by Bioz Stars, 2020-09
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    91
    Cell Signaling Technology Inc α sma antibody
    Silencing of SNHG7 inhibited cardiac remodeling after MI. ( A ) qRT-PCR analysis showing that AAV9-sh-SNHG7 reversed the up-regulation of SNHG7 in MI mice; GAPDH mRNA served as an internal control, and AAV9-sh-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ( B , C ) 4 weeks after MI, echocardiographic findings showed that the silencing of SNHG7 improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. ( D ) mRNA expression of collagen 1α1 and collagen 3α1 were measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=10 mice per group. ( E ) Western blotting analysis showing elevation of protein levels of collagen 1 and <t>α-SMA</t> in peri-infarcted tissue of left ventricle of mice after MI. Data was presented mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 mice per group. ** P
    α Sma Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α sma antibody/product/Cell Signaling Technology Inc
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    α sma antibody - by Bioz Stars, 2020-09
    91/100 stars
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    Downregulation of p53 expression suppresses the pro-apoptotic effect of liriodenine. (A) Western blot analysis revealing the expression of p53 at different times following treatment with 10 μM liriodenine. β-actin was used as the internal control. Blots were repeated at least three times for statistical analysis. (B) Statistical analysis of p53 expression at different times following treatment with 10 μm liriodenine. ** P

    Journal: Oncology Letters

    Article Title: Liriodenine induces the apoptosis of human laryngocarcinoma cells via the upregulation of p53 expression

    doi: 10.3892/ol.2014.2834

    Figure Lengend Snippet: Downregulation of p53 expression suppresses the pro-apoptotic effect of liriodenine. (A) Western blot analysis revealing the expression of p53 at different times following treatment with 10 μM liriodenine. β-actin was used as the internal control. Blots were repeated at least three times for statistical analysis. (B) Statistical analysis of p53 expression at different times following treatment with 10 μm liriodenine. ** P

    Article Snippet: The rabbit anti-human monoclonal p53 (1:500 dilution), polyclonal -vascular epidermal growth factor (VEGF; 1:1,000 dilution), polyclonal -cleaved caspase-3 (1:500 dilution) and monoclonal -β-actin (1:5,000 dilution) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

    Techniques: Expressing, Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-9 (cleaved caspase-9) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase-9 (35 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-9 (cleaved caspase-9) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase-9 (35 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein levels of Bax and Bcl2 in the rat kidney tissue. (A) Representative Western blots showing specific bands for Bax, Bcl2 and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein levels of Bax and Bcl2 in the rat kidney tissue. (A) Representative Western blots showing specific bands for Bax, Bcl2 and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase 8 (cleaved caspase 8) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase 8 (10 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase 8 (cleaved caspase 8) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase 8 (10 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein levels of Bax and Bcl2 in the rat liver tissue. (A) Representative Western blots showing specific bands for Bax, Bcl2 and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein levels of Bax and Bcl2 in the rat liver tissue. (A) Representative Western blots showing specific bands for Bax, Bcl2 and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat liver tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole liver homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-8 (cleaved caspase-8) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-8 (10 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-8 (cleaved caspase-8) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-8 (10 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. ** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-9 (cleaved caspase-9) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-9 (35 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-9 (cleaved caspase-9) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-9 (35 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Effect of Nigella sativa fixed oil on ethanol toxicity in rats

    doi:

    Figure Lengend Snippet: Effect of NSO (0.5 ml/kg) and ethanol on the protein level of caspase-3 (cleaved caspase-3) in the rat kidney tissue. (A) Representative Western blots showing specific bands for cleaved caspase-3 (17 KDa) and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from whole kidney homogenate were applied in each lane. (B) Densitometric data of protein analysis. Data are expressed as the mean ± SEM. *** P

    Article Snippet: The primary antibodies were rabbit monoclonal anti-serum against Bcl2 (Cell Signaling, #2870), rabbit monoclonal anti-serum against caspase-3 (Cell Signaling, #9665), rabbit monoclonal anti-serum against caspase-8 (Cell Signaling, #4790), rabbit monoclonal anti-serum against caspase-9 (Cell Signaling, #9506) and rabbit polyclonal anti-serum against Bax (Cell Signaling, #2772) and mouse monoclonal anti serum against β-actin (Cell Signaling, # 3700).

    Techniques: Western Blot

    A : anti-phosphorylated (p)TRPC6 (Thr 69 ) recognized pThr 70 in HEK-293 cells expressing hTRPC6 WT or hTRPC6 S322A but not cells expressing hTRPC6 T70A . B : anti-pTRPC6 (Thr 69 ) antibody detected pTRPC6 in the whole kidney and mouse primary podocyte lysates. Whole kidney protein extract ( left ) and primary podocyte cell lysates ( right ) from WT and TRPC6-deficient knockout (KO) mice were stained for pTRPC6, TRPC6, and β-Actin. C : time course of TRPC6 dephosphorylation in response to chronic ANG II stimulation. ANG II dephosphorylation occurred within 6 h of the initiation of ANG II treatment.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Phosphodiesterase 5 inhibition ameliorates angiontensin II-induced podocyte dysmotility via the protein kinase G-mediated downregulation of TRPC6 activity

    doi: 10.1152/ajprenal.00212.2013

    Figure Lengend Snippet: A : anti-phosphorylated (p)TRPC6 (Thr 69 ) recognized pThr 70 in HEK-293 cells expressing hTRPC6 WT or hTRPC6 S322A but not cells expressing hTRPC6 T70A . B : anti-pTRPC6 (Thr 69 ) antibody detected pTRPC6 in the whole kidney and mouse primary podocyte lysates. Whole kidney protein extract ( left ) and primary podocyte cell lysates ( right ) from WT and TRPC6-deficient knockout (KO) mice were stained for pTRPC6, TRPC6, and β-Actin. C : time course of TRPC6 dephosphorylation in response to chronic ANG II stimulation. ANG II dephosphorylation occurred within 6 h of the initiation of ANG II treatment.

    Article Snippet: Protein immunoblot analysis was then performed using rabbit polyclonal anti-TRPC6 antibody (Abcam, Cambridge, MA), rabbit polycolonal anti-phospho-TRPC6 [pTRPC6 (Thr69 )] antibody commercially generated by Biomatik (Wilmington, DE) using the published sequence reported by Nishida et al. , and rabbit anti-β-actin antibody (Cell Signaling Technologies).

    Techniques: Expressing, Knock-Out, Mouse Assay, Staining, De-Phosphorylation Assay

    Silencing of SNHG7 inhibited cardiac remodeling after MI. ( A ) qRT-PCR analysis showing that AAV9-sh-SNHG7 reversed the up-regulation of SNHG7 in MI mice; GAPDH mRNA served as an internal control, and AAV9-sh-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ( B , C ) 4 weeks after MI, echocardiographic findings showed that the silencing of SNHG7 improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. ( D ) mRNA expression of collagen 1α1 and collagen 3α1 were measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=10 mice per group. ( E ) Western blotting analysis showing elevation of protein levels of collagen 1 and α-SMA in peri-infarcted tissue of left ventricle of mice after MI. Data was presented mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 mice per group. ** P

    Journal: Aging (Albany NY)

    Article Title: LncRNA SNHG7 promotes cardiac remodeling by upregulating ROCK1 via sponging miR-34-5p

    doi: 10.18632/aging.103269

    Figure Lengend Snippet: Silencing of SNHG7 inhibited cardiac remodeling after MI. ( A ) qRT-PCR analysis showing that AAV9-sh-SNHG7 reversed the up-regulation of SNHG7 in MI mice; GAPDH mRNA served as an internal control, and AAV9-sh-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ( B , C ) 4 weeks after MI, echocardiographic findings showed that the silencing of SNHG7 improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. ( D ) mRNA expression of collagen 1α1 and collagen 3α1 were measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=10 mice per group. ( E ) Western blotting analysis showing elevation of protein levels of collagen 1 and α-SMA in peri-infarcted tissue of left ventricle of mice after MI. Data was presented mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 mice per group. ** P

    Article Snippet: The antibodies used were listed as follows:collagen 1 antibody was produced by Proteintech Group (Wuhan, China), α-SMA antibody by Cell Signaling Technology (Danvers, MA, USA), secondary antibodies IRDye700 (mouse) or IRDye800 (rabbit) by LICOR (Lincoln, Nebraska, USA).

    Techniques: Quantitative RT-PCR, Mouse Assay, Expressing, Western Blot

    Silencing of lncRNA SNHG7 alleviated TGF-β1-induced fibrogenesis in cardiac fibroblasts. ( A , B ) Suppression of SNHG7 attenuated the increase in collagen 1 α 1 and collagen 3 α 1 expression induced by TGF-β1, as measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( C ) Western blotting analysis showing that knockdown of SNHG7 attenuated TGF-β1-induced fibrotic protein expression (Collagen I and α-SMA); GAPDH served as a loading control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( D ) MTT assay for the assessment of cell viability. Transfection of si-SNHG7 with or without AMO-34-5p in cardiac fibroblasts treated with TGF-β1 for 24h. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( E ) EdU staining for the assessment of cell proliferation in cardiac fibroblasts inhibiting SNHG7 in the presence or absence of AMO-34-5p mimics. Scale bars represented 50 μm. ( F ) Representative images of immunofluorescence staining showing that knockdown of SNHG7 abated the TGF-β1-induced fibroblast-myofibroblast transition, which was promoted by AMO-34-5p. Scale bars represented 50 μm. ** P

    Journal: Aging (Albany NY)

    Article Title: LncRNA SNHG7 promotes cardiac remodeling by upregulating ROCK1 via sponging miR-34-5p

    doi: 10.18632/aging.103269

    Figure Lengend Snippet: Silencing of lncRNA SNHG7 alleviated TGF-β1-induced fibrogenesis in cardiac fibroblasts. ( A , B ) Suppression of SNHG7 attenuated the increase in collagen 1 α 1 and collagen 3 α 1 expression induced by TGF-β1, as measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( C ) Western blotting analysis showing that knockdown of SNHG7 attenuated TGF-β1-induced fibrotic protein expression (Collagen I and α-SMA); GAPDH served as a loading control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( D ) MTT assay for the assessment of cell viability. Transfection of si-SNHG7 with or without AMO-34-5p in cardiac fibroblasts treated with TGF-β1 for 24h. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( E ) EdU staining for the assessment of cell proliferation in cardiac fibroblasts inhibiting SNHG7 in the presence or absence of AMO-34-5p mimics. Scale bars represented 50 μm. ( F ) Representative images of immunofluorescence staining showing that knockdown of SNHG7 abated the TGF-β1-induced fibroblast-myofibroblast transition, which was promoted by AMO-34-5p. Scale bars represented 50 μm. ** P

    Article Snippet: The antibodies used were listed as follows:collagen 1 antibody was produced by Proteintech Group (Wuhan, China), α-SMA antibody by Cell Signaling Technology (Danvers, MA, USA), secondary antibodies IRDye700 (mouse) or IRDye800 (rabbit) by LICOR (Lincoln, Nebraska, USA).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, MTT Assay, Transfection, Staining, Immunofluorescence

    Overexpression of miR-34-5p ameliorated cardiac fibrosis in the mice after MI. ( A ) Intravenous injection of AAV9-miR-34-5p via tail increased miR-34-5p expression in normal mice, as measured by qRT-PCR; GAPDH served as an internal control, and AAV9-scramble served as a negative control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. ( B , C ) Four weeks after MI, echocardiographic imaging showed that the overexpression of miR-34-5p improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. ( D ) qRT-PCR analysis showing that AAV9-miR-34-5p injection reversed the up-regulation of collagen 1α1 and collagen 3α1 in MI mice; GAPDH mRNA served as an internal control, and AAV9-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ( E ) Protein levels of collagen 1 and α-SMA were measured by western blot; GAPDH served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ** P

    Journal: Aging (Albany NY)

    Article Title: LncRNA SNHG7 promotes cardiac remodeling by upregulating ROCK1 via sponging miR-34-5p

    doi: 10.18632/aging.103269

    Figure Lengend Snippet: Overexpression of miR-34-5p ameliorated cardiac fibrosis in the mice after MI. ( A ) Intravenous injection of AAV9-miR-34-5p via tail increased miR-34-5p expression in normal mice, as measured by qRT-PCR; GAPDH served as an internal control, and AAV9-scramble served as a negative control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. ( B , C ) Four weeks after MI, echocardiographic imaging showed that the overexpression of miR-34-5p improved ejection fraction (EF) and fraction shortening (FS). Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=12 mice per group. ( D ) qRT-PCR analysis showing that AAV9-miR-34-5p injection reversed the up-regulation of collagen 1α1 and collagen 3α1 in MI mice; GAPDH mRNA served as an internal control, and AAV9-scramble served as an additional control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ( E ) Protein levels of collagen 1 and α-SMA were measured by western blot; GAPDH served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 mice per group. ** P

    Article Snippet: The antibodies used were listed as follows:collagen 1 antibody was produced by Proteintech Group (Wuhan, China), α-SMA antibody by Cell Signaling Technology (Danvers, MA, USA), secondary antibodies IRDye700 (mouse) or IRDye800 (rabbit) by LICOR (Lincoln, Nebraska, USA).

    Techniques: Over Expression, Mouse Assay, Injection, Expressing, Quantitative RT-PCR, Negative Control, Two Tailed Test, Imaging, Western Blot

    The differential expression of SNHG7 in cardiac tissues and cardiac fibroblast. ( A ) Quantification of the total fibrotic area using Image-J. Fibrosis areas of sham-operated group and MI group were detected by Masson staining. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=7 mice per group. ( B ) mRNA expression of collagen 1α1 and collagen 3α1 were measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=6 mice per group. ( C ) Protein levels of collagen 1 and α-SMA were measured by western blotting analysis; GAPDH served as an internal control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=6 mice per group. ( D ) qRT-PCR analysis showing upregulation of lncRNA SNHG7 in the peri-infarcted and infarcted areas of left ventricle of mice after MI. Data was presented mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 mice per group. ( E ) qRT-PCR analysis showing elevation of lncRNA SNHG7 in cardiac fibroblasts after treatment with TGF-β1 (10 ng/mL) for 24h. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. ** P

    Journal: Aging (Albany NY)

    Article Title: LncRNA SNHG7 promotes cardiac remodeling by upregulating ROCK1 via sponging miR-34-5p

    doi: 10.18632/aging.103269

    Figure Lengend Snippet: The differential expression of SNHG7 in cardiac tissues and cardiac fibroblast. ( A ) Quantification of the total fibrotic area using Image-J. Fibrosis areas of sham-operated group and MI group were detected by Masson staining. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=7 mice per group. ( B ) mRNA expression of collagen 1α1 and collagen 3α1 were measured by qRT-PCR; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=6 mice per group. ( C ) Protein levels of collagen 1 and α-SMA were measured by western blotting analysis; GAPDH served as an internal control. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=6 mice per group. ( D ) qRT-PCR analysis showing upregulation of lncRNA SNHG7 in the peri-infarcted and infarcted areas of left ventricle of mice after MI. Data was presented mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 mice per group. ( E ) qRT-PCR analysis showing elevation of lncRNA SNHG7 in cardiac fibroblasts after treatment with TGF-β1 (10 ng/mL) for 24h. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. ** P

    Article Snippet: The antibodies used were listed as follows:collagen 1 antibody was produced by Proteintech Group (Wuhan, China), α-SMA antibody by Cell Signaling Technology (Danvers, MA, USA), secondary antibodies IRDye700 (mouse) or IRDye800 (rabbit) by LICOR (Lincoln, Nebraska, USA).

    Techniques: Expressing, Staining, Two Tailed Test, Mouse Assay, Quantitative RT-PCR, Western Blot

    ROCK1 was a direct target of miR-34-5p and mediated the anti-fibrotic function of miR-34-5p. ( A ) The predicted binding sites of ROCK1 and miR-34-5p. ( B ) Luciferase reporter activities of chimeric vectors carrying the luciferase gene and a fragment of the 3’ UTR of ROCK1 containing the wild type or mutant miR-34-5p binding sites. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. ( C , D ) qRT-PCR analysis showing that overexpression of miR-34-5p inhibited the mRNA level of ROCK1 and AMO-34-5p transfection elevated the mRNA level of ROCK1; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( E ) Protein levels of collagen 1 and α-SMA were measured by western blotting; GAPDH served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 independent cell cultures. ( F ) Representative images of immunofluorescence staining showing that overexpression of miR-34-5p diminished fibroblast-myofibroblast transition induced by forced expression of ROCK1. Scale bars represented 50 μm. ** P

    Journal: Aging (Albany NY)

    Article Title: LncRNA SNHG7 promotes cardiac remodeling by upregulating ROCK1 via sponging miR-34-5p

    doi: 10.18632/aging.103269

    Figure Lengend Snippet: ROCK1 was a direct target of miR-34-5p and mediated the anti-fibrotic function of miR-34-5p. ( A ) The predicted binding sites of ROCK1 and miR-34-5p. ( B ) Luciferase reporter activities of chimeric vectors carrying the luciferase gene and a fragment of the 3’ UTR of ROCK1 containing the wild type or mutant miR-34-5p binding sites. Data was presented as mean ± SEM; two-tailed t test was used for the statistical analysis. n=5 independent cell cultures. ( C , D ) qRT-PCR analysis showing that overexpression of miR-34-5p inhibited the mRNA level of ROCK1 and AMO-34-5p transfection elevated the mRNA level of ROCK1; GAPDH mRNA served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=5 independent cell cultures. ( E ) Protein levels of collagen 1 and α-SMA were measured by western blotting; GAPDH served as an internal control. Data was presented as mean ± SEM; one-way ANOVA was used for the statistical analysis. n=6 independent cell cultures. ( F ) Representative images of immunofluorescence staining showing that overexpression of miR-34-5p diminished fibroblast-myofibroblast transition induced by forced expression of ROCK1. Scale bars represented 50 μm. ** P

    Article Snippet: The antibodies used were listed as follows:collagen 1 antibody was produced by Proteintech Group (Wuhan, China), α-SMA antibody by Cell Signaling Technology (Danvers, MA, USA), secondary antibodies IRDye700 (mouse) or IRDye800 (rabbit) by LICOR (Lincoln, Nebraska, USA).

    Techniques: Binding Assay, Luciferase, Mutagenesis, Two Tailed Test, Quantitative RT-PCR, Over Expression, Transfection, Western Blot, Immunofluorescence, Staining, Expressing