Journal: Biomedical Reports
Article Title: Suppression of the increasing level of acetylcholine-stimulated intracellular Ca2+ in guinea pig airway smooth muscle cells by mabuterol
Figure Lengend Snippet: Data of (A and B) immunocytochemical identification of the cells preloaded with α-smooth muscle actin and (C and D) immunofluorescent identification of the cells preloaded with fluorescein isothiocyanate. The images in (A) and (B) were obtained under an inverted system microscope (magnification, x200 and x400, respectively) and those in (C) and (D) under an inverted fluorescent microscope (magnification, x200 and x400, respectively) in the condition of absorption peak at 492 nm and emission peak at 520 nm. The magnified cells in (B) are various shapes, including irregular triangle and fusiform, are illustrated with a thin arrow and common arrow, respectively. The arrow in (D) also indicates airway smooth muscle cells in fusiform identified with immunofluorescent staining.
Article Snippet: Mouse anti-α-smooth muscle actin (α-SMA), 5% bovine serum albumin (BSA), streptavidin-biotin complex (SABC) immunohistochemical staining kit and 3,3′ diaminobenzidine (DAB) chromogenic reagent kit were all purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China).
Techniques: Microscopy, Staining