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Biochrom mononuclear cells mnc
Mononuclear Cells Mnc, supplied by Biochrom, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 17 article reviews
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mononuclear cells mnc - by Bioz Stars, 2020-09
91/100 stars

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Flow Cytometry:

Article Title: Bispecific antibody releasing-mesenchymal stromal cell machinery for retargeting T cells towards acute myeloid leukemia blasts
Article Snippet: .. Flow cytometry killing assay with fresh AML samples Upon approval by the local institutional review board, mononuclear cell (MNC) samples of consenting AML patients with hyperleukocytosis were prepared by gradient centrifugation over polydextran based separating solution Biocoll (Biochrom). .. The redirection of autologous T cells towards AML blasts by the bsAb-releasing hMSCs was investigated by co-cultivation of 1 × 104 pre-cultured (48 h), genetically modified hMSCs with 1 × 105 AML patient-derived MNCs.

Isolation:

Article Title: The prognostic and predictive value of IKZF1 and IKZF3 expression in T-cells in patients with multiple myeloma
Article Snippet: .. To analyse the expression of IKZF1/3 proteins in T-cells, Mononuclear cells (MNC) freshly isolated from the PB/BM using ficoll-hypaque density gradient centrifugation (Biochrom) were frozen in freezing medium (90% heat inactivated fetal calf serum (FCS) from PAA Laboratories, Germany, and 10% Dimethyl sulfoxide (DMSO) from SERVA Electrophoresis GmbH) in cryogenic vials. ..

Article Title: The dual PPARα/γ agonist aleglitazar increases the number and function of endothelial progenitor cells: implications for vascular function and atherogenesis
Article Snippet: .. To select for EPC, mononuclear cells (MNC) were isolated from spleen homogenates from WT, ApoE−/− and eNOS−/− mice by Ficoll density gradient centrifugation (Biocoll Separating Solution; Biochrom, Berlin, Germany) and cultured on fibronectin-coated culture dishes in endothelial basal medium (EBM; Lonza, Wuppertal, Germany) with supplements (1 μg·mL−1 hydrocortisone, 3 μg·mL−1 bovine brain extract, 30 μg·mL−1 gentamicin, 50 μg·mL−1 amphotericin B, 10 μg·mL−1 human endothelial growth factor and 20% fetal calf serum; Gensch et al ., ; Pöss et al ., ; Werner et al ., ). ..

Article Title: Synergistic effects of telmisartan and simvastatin on endothelial progenitor cells
Article Snippet: .. Briefly, mononuclear cells (MNC) were isolated using Ficoll density gradient (Biocoll, Biochrom, Berlin, Germany) and seeded into 24-well tissue culture plates pre-coated with human fibronectin (Sigma, Steinheim am Albuch, Germany) at 4 × 106 (acLDL/lectin staining) or 1 × 106 (EC-CFU) cells/ml in endothelial basal medium (EBM) and supplements as recommended by the manufacturer (Promocell, Heidelberg, Germany). .. Stimulation of EPC Cells were incubated for 72 hrs with telmisartan in concentrations of 1, 10 or 100 μmol/l; angiotensin II 1 μmol/l; telmisartan 10 μmol/l and angiotensin II 1 μmol/l; simvastatin 1 μmol/l; telmisartan 10 or 100 μmol/l and simvastatin 1 μmol/l; GW9662 5 μmol/l; PD123319 1 μmol/l; TNFα 25 ng/well.

Article Title: Donor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells
Article Snippet: .. Briefly, bone fragments were flushed with PBS and mononuclear cells (MNC) were subsequently isolated by density gradient on Biocoll (Biochrom AG, Berlin, Germany). .. Adipose tissue (AT)-derived MSC were isolated from lipoaspirates that were washed in 9 g/L NaCl, centrifuged at 300×g for 10 minutes and the middle layer was subsequently digested with 2 g/L collagenase type I (Biochrom; specific activity 125–250 Mandl Units/mL of powder substance) for 45 min at 37°C on a horizontal rotary shaker .

Cytometry:

Article Title: Bispecific antibody releasing-mesenchymal stromal cell machinery for retargeting T cells towards acute myeloid leukemia blasts
Article Snippet: .. Flow cytometry killing assay with fresh AML samples Upon approval by the local institutional review board, mononuclear cell (MNC) samples of consenting AML patients with hyperleukocytosis were prepared by gradient centrifugation over polydextran based separating solution Biocoll (Biochrom). .. The redirection of autologous T cells towards AML blasts by the bsAb-releasing hMSCs was investigated by co-cultivation of 1 × 104 pre-cultured (48 h), genetically modified hMSCs with 1 × 105 AML patient-derived MNCs.

Cell Culture:

Article Title: The dual PPARα/γ agonist aleglitazar increases the number and function of endothelial progenitor cells: implications for vascular function and atherogenesis
Article Snippet: .. To select for EPC, mononuclear cells (MNC) were isolated from spleen homogenates from WT, ApoE−/− and eNOS−/− mice by Ficoll density gradient centrifugation (Biocoll Separating Solution; Biochrom, Berlin, Germany) and cultured on fibronectin-coated culture dishes in endothelial basal medium (EBM; Lonza, Wuppertal, Germany) with supplements (1 μg·mL−1 hydrocortisone, 3 μg·mL−1 bovine brain extract, 30 μg·mL−1 gentamicin, 50 μg·mL−1 amphotericin B, 10 μg·mL−1 human endothelial growth factor and 20% fetal calf serum; Gensch et al ., ; Pöss et al ., ; Werner et al ., ). ..

Mouse Assay:

Article Title: The dual PPARα/γ agonist aleglitazar increases the number and function of endothelial progenitor cells: implications for vascular function and atherogenesis
Article Snippet: .. To select for EPC, mononuclear cells (MNC) were isolated from spleen homogenates from WT, ApoE−/− and eNOS−/− mice by Ficoll density gradient centrifugation (Biocoll Separating Solution; Biochrom, Berlin, Germany) and cultured on fibronectin-coated culture dishes in endothelial basal medium (EBM; Lonza, Wuppertal, Germany) with supplements (1 μg·mL−1 hydrocortisone, 3 μg·mL−1 bovine brain extract, 30 μg·mL−1 gentamicin, 50 μg·mL−1 amphotericin B, 10 μg·mL−1 human endothelial growth factor and 20% fetal calf serum; Gensch et al ., ; Pöss et al ., ; Werner et al ., ). ..

Expressing:

Article Title: The prognostic and predictive value of IKZF1 and IKZF3 expression in T-cells in patients with multiple myeloma
Article Snippet: .. To analyse the expression of IKZF1/3 proteins in T-cells, Mononuclear cells (MNC) freshly isolated from the PB/BM using ficoll-hypaque density gradient centrifugation (Biochrom) were frozen in freezing medium (90% heat inactivated fetal calf serum (FCS) from PAA Laboratories, Germany, and 10% Dimethyl sulfoxide (DMSO) from SERVA Electrophoresis GmbH) in cryogenic vials. ..

Lysis:

Article Title: Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1
Article Snippet: .. Briefly, mononuclear cells (MNC) of one cord blood were obtained by Ficoll gradient separation (density 1.077 g/cm3 ; Biochrom AG; Berlin, Germany) followed by lysis of remaining erythrocytes with ammonium chloride (pharmacy of the university medical centre Duesseldorf). .. 5–7∗106 CB MNC/mL were cultured in DMEM low glucose (Lonza Inc.; Allendale, NJ, USA) with 30% FCS (Perbio/Fisher Scientific; Bonn, Germany), 1∗10−7 M dexamethasone (Sigma-Aldrich; St.Louis; MO, USA), penicillin/streptomycin and L-glutamine (PSG; all Lonza Inc.) until detection of adherent growing colonies.

Staining:

Article Title: Synergistic effects of telmisartan and simvastatin on endothelial progenitor cells
Article Snippet: .. Briefly, mononuclear cells (MNC) were isolated using Ficoll density gradient (Biocoll, Biochrom, Berlin, Germany) and seeded into 24-well tissue culture plates pre-coated with human fibronectin (Sigma, Steinheim am Albuch, Germany) at 4 × 106 (acLDL/lectin staining) or 1 × 106 (EC-CFU) cells/ml in endothelial basal medium (EBM) and supplements as recommended by the manufacturer (Promocell, Heidelberg, Germany). .. Stimulation of EPC Cells were incubated for 72 hrs with telmisartan in concentrations of 1, 10 or 100 μmol/l; angiotensin II 1 μmol/l; telmisartan 10 μmol/l and angiotensin II 1 μmol/l; simvastatin 1 μmol/l; telmisartan 10 or 100 μmol/l and simvastatin 1 μmol/l; GW9662 5 μmol/l; PD123319 1 μmol/l; TNFα 25 ng/well.

Gradient Centrifugation:

Article Title: Bispecific antibody releasing-mesenchymal stromal cell machinery for retargeting T cells towards acute myeloid leukemia blasts
Article Snippet: .. Flow cytometry killing assay with fresh AML samples Upon approval by the local institutional review board, mononuclear cell (MNC) samples of consenting AML patients with hyperleukocytosis were prepared by gradient centrifugation over polydextran based separating solution Biocoll (Biochrom). .. The redirection of autologous T cells towards AML blasts by the bsAb-releasing hMSCs was investigated by co-cultivation of 1 × 104 pre-cultured (48 h), genetically modified hMSCs with 1 × 105 AML patient-derived MNCs.

Article Title: The prognostic and predictive value of IKZF1 and IKZF3 expression in T-cells in patients with multiple myeloma
Article Snippet: .. To analyse the expression of IKZF1/3 proteins in T-cells, Mononuclear cells (MNC) freshly isolated from the PB/BM using ficoll-hypaque density gradient centrifugation (Biochrom) were frozen in freezing medium (90% heat inactivated fetal calf serum (FCS) from PAA Laboratories, Germany, and 10% Dimethyl sulfoxide (DMSO) from SERVA Electrophoresis GmbH) in cryogenic vials. ..

Article Title: Perforin-independent rejection of transplanted human stem cells
Article Snippet: .. Mononuclear cells (MNC) were obtained from discarded umbilical cord blood (UCB) samples by density gradient centrifugation using Biocoll separating solution (Biochrom AG, Berlin, Germany). .. CD34+ cells were obtained by double selection with MACS columns using the CD34 Progenitor Isolation Kit (Miltenyi Biotech, Bergisch-Gladbach, Germany).

Article Title: Serum after Autologous Transplantation Stimulates Proliferation and Expansion of Human Hematopoietic Progenitor Cells
Article Snippet: .. Mononuclear cells (MNC) were separated by density gradient centrifugation on Biocoll (Biochrom KG, Berlin, Germany) and CD34+ cells were enriched using the human CD34 Micro Bead Kit on a MiniMACS system according to the manufacturer's instructions (Miltenyi Biotec GmbH, Bergisch-Gladbach, Germany) as described before . .. Isolation of mesenchymal stromal cells from human bone marrow MSC were isolated from the caput femoris after the patient's written consent using guidelines specifically approved by the Ethic Committee of RWTH Aachen University (Permit Number: EK128/09).

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  • 85
    Biochrom cfse labelled pbmcs
    Analysis for proliferation of CD4+ T cells after delivery of the TT-derived T-cell epitope to slanDCs via the novel immuno targeting system. (A) <t>CFSE</t> labeled <t>PBMCs</t> were prepared and incubated at 37°C for eight days with either full length TT (b) or the linker module containing (d) or lacking (c) the TT-derived T-cell epitope TT p and analysed by FACS. The data indicate that PBMCs of the selected donor contain anti-TT memory T cells that can be recalled by full length TT and to a less extent by the TT p peptide linker but not by the linker peptide lacking the TT p epitope. Untreated PBMCs (a). (B) CFSE labelled PBMCs were incubated with either the antigen-containing scaffold (c) or the single components (a,b) at 4°C. Unbound material was removed by washing.
    Cfse Labelled Pbmcs, supplied by Biochrom, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfse labelled pbmcs/product/Biochrom
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cfse labelled pbmcs - by Bioz Stars, 2020-09
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    88
    Biochrom pbmc isolation peripheral blood mononuclear cells pbmcs
    Elevated Tim-3 expression on circulating CD4 and CD8 T cells in HBV-infected individuals . (A) <t>PBMCs</t> from normal controls, AHB patients (early phase), MCHB and SCHB patients were stained with antibodies against CD3, CD4/CD8, and Tim-3. An isotype-matched antibody was used as a negative control. (B) The percentage of Tim-3 cells within CD4+ and CD8+ T cell populations are increased in AHB and CHB patients and further enhanced in the severe stage of CHB. Each dot represents an individual data point and the horizontal lines represent the mean. The Mann-Whitney U test was used to compare differences among groups. (C) There is a significantly positive correlation between the frequency of Tim-3-expressing CD4 + and CD8 + T cells in the studied subjects. Spearman test was performed for correlation analysis.
    Pbmc Isolation Peripheral Blood Mononuclear Cells Pbmcs, supplied by Biochrom, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc isolation peripheral blood mononuclear cells pbmcs/product/Biochrom
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc isolation peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
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    94
    Biochrom pbmcs
    Activation of allogeneic T cells by <t>DCs</t> . <t>PBMCs</t> from healthy unrelated donors were stained with CFSE and cocultured with DCs at a ratio of 10:1. After 6 days, proliferation was assessed by flow cytometry. (A) Representative examples of unstimulated cells and proliferating cells are shown in the presence of PHA (3 μg/mL) and after coculture with DCs generated from HCs and two AML patients (AML #5, AML #12). (B) Stimulation index of DCs generated from HCs and AML patients with cocktail C, CP, R, and RP (n = 3-9) are summarized as mean ± SD.
    Pbmcs, supplied by Biochrom, used in various techniques. Bioz Stars score: 94/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Biochrom
    Average 94 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    94/100 stars
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    Analysis for proliferation of CD4+ T cells after delivery of the TT-derived T-cell epitope to slanDCs via the novel immuno targeting system. (A) CFSE labeled PBMCs were prepared and incubated at 37°C for eight days with either full length TT (b) or the linker module containing (d) or lacking (c) the TT-derived T-cell epitope TT p and analysed by FACS. The data indicate that PBMCs of the selected donor contain anti-TT memory T cells that can be recalled by full length TT and to a less extent by the TT p peptide linker but not by the linker peptide lacking the TT p epitope. Untreated PBMCs (a). (B) CFSE labelled PBMCs were incubated with either the antigen-containing scaffold (c) or the single components (a,b) at 4°C. Unbound material was removed by washing.

    Journal: PLoS ONE

    Article Title: A Novel Modular Antigen Delivery System for Immuno Targeting of Human 6-sulfo LacNAc-Positive Blood Dendritic Cells (SlanDCs)

    doi: 10.1371/journal.pone.0016315

    Figure Lengend Snippet: Analysis for proliferation of CD4+ T cells after delivery of the TT-derived T-cell epitope to slanDCs via the novel immuno targeting system. (A) CFSE labeled PBMCs were prepared and incubated at 37°C for eight days with either full length TT (b) or the linker module containing (d) or lacking (c) the TT-derived T-cell epitope TT p and analysed by FACS. The data indicate that PBMCs of the selected donor contain anti-TT memory T cells that can be recalled by full length TT and to a less extent by the TT p peptide linker but not by the linker peptide lacking the TT p epitope. Untreated PBMCs (a). (B) CFSE labelled PBMCs were incubated with either the antigen-containing scaffold (c) or the single components (a,b) at 4°C. Unbound material was removed by washing.

    Article Snippet: Medium used for culturing of CFSE-labelled PBMCs consisted of RPMI 1640 (Biochrom, Berlin, Germany) supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 1% (v/v) nonessential amino acids, 100 µg/ml penicillin, 100 µg/ml streptomycin (all from Biochrom, Berlin, Germany) and 10% (v/v) human serum (CC pro, Neustadt, Germany).

    Techniques: Derivative Assay, Labeling, Incubation, FACS

    Elevated Tim-3 expression on circulating CD4 and CD8 T cells in HBV-infected individuals . (A) PBMCs from normal controls, AHB patients (early phase), MCHB and SCHB patients were stained with antibodies against CD3, CD4/CD8, and Tim-3. An isotype-matched antibody was used as a negative control. (B) The percentage of Tim-3 cells within CD4+ and CD8+ T cell populations are increased in AHB and CHB patients and further enhanced in the severe stage of CHB. Each dot represents an individual data point and the horizontal lines represent the mean. The Mann-Whitney U test was used to compare differences among groups. (C) There is a significantly positive correlation between the frequency of Tim-3-expressing CD4 + and CD8 + T cells in the studied subjects. Spearman test was performed for correlation analysis.

    Journal: Virology Journal

    Article Title: Tim-3 expression on peripheral T cell subsets correlates with disease progression in hepatitis B infection

    doi: 10.1186/1743-422X-8-113

    Figure Lengend Snippet: Elevated Tim-3 expression on circulating CD4 and CD8 T cells in HBV-infected individuals . (A) PBMCs from normal controls, AHB patients (early phase), MCHB and SCHB patients were stained with antibodies against CD3, CD4/CD8, and Tim-3. An isotype-matched antibody was used as a negative control. (B) The percentage of Tim-3 cells within CD4+ and CD8+ T cell populations are increased in AHB and CHB patients and further enhanced in the severe stage of CHB. Each dot represents an individual data point and the horizontal lines represent the mean. The Mann-Whitney U test was used to compare differences among groups. (C) There is a significantly positive correlation between the frequency of Tim-3-expressing CD4 + and CD8 + T cells in the studied subjects. Spearman test was performed for correlation analysis.

    Article Snippet: PBMC isolation Peripheral blood mononuclear cells (PBMCs) were isolated from fresh-heparinized blood by standard Ficoll-Hypaque density centrifugation (Biochrom, Berline, Germany).

    Techniques: Expressing, Infection, Staining, Negative Control, MANN-WHITNEY

    Activation of allogeneic T cells by DCs . PBMCs from healthy unrelated donors were stained with CFSE and cocultured with DCs at a ratio of 10:1. After 6 days, proliferation was assessed by flow cytometry. (A) Representative examples of unstimulated cells and proliferating cells are shown in the presence of PHA (3 μg/mL) and after coculture with DCs generated from HCs and two AML patients (AML #5, AML #12). (B) Stimulation index of DCs generated from HCs and AML patients with cocktail C, CP, R, and RP (n = 3-9) are summarized as mean ± SD.

    Journal: Journal of Translational Medicine

    Article Title: Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission

    doi: 10.1186/1479-5876-9-151

    Figure Lengend Snippet: Activation of allogeneic T cells by DCs . PBMCs from healthy unrelated donors were stained with CFSE and cocultured with DCs at a ratio of 10:1. After 6 days, proliferation was assessed by flow cytometry. (A) Representative examples of unstimulated cells and proliferating cells are shown in the presence of PHA (3 μg/mL) and after coculture with DCs generated from HCs and two AML patients (AML #5, AML #12). (B) Stimulation index of DCs generated from HCs and AML patients with cocktail C, CP, R, and RP (n = 3-9) are summarized as mean ± SD.

    Article Snippet: Cell isolation and generation of mature DCs from PBMCs In brief, PBMCs were isolated by Ficoll/Hypaque (Biochrom, Berlin, Germany) density gradient centrifugation.

    Techniques: Activation Assay, Staining, Flow Cytometry, Cytometry, Generated

    Activation of NK cells by DCs . Allogeneic and autologous non-adherent PBMCs were cocultured with DCs at a ratio of 10:1 for 24 h. Cells were stained for CD3, CD56, CD69 and IFN-γ expression and analyzed by flow cytometry. (A) Shown is the percentage of CD69 expression on CD3 - /CD56 + gated NK cells. Representative histograms of unstimulated NK cells, IL-2 (500 IU/mL) activated NK cells, DCs + autologous NK cells (upper panel) and DCs + allogeneic NK cells (lower panel) of one AML patient are shown (cocktail R was used for DC maturation). The dot blots below show the corresponding intracellular IFN-γ staining, with PMA/ionomycin serving as a positive control. (B) Summary of the percentage of CD69 expression on CD3 - /CD56 + NK cells after coculture with allogeneic and autologous NK cells from HC and AML patients. (C) Production of IFN-γ after coculture of DCs and isolated NK cells was assessed by ELISA. DCs were generated and cocultured with allogeneic NK cells at a ratio of 1:10 for 24 h. Supernatant was analyzed by standard ELISA for IFN-γ. The comparison of HC and AML patients is shown using the four different maturation cocktails. As a positive control NK cells were stimulated with IL-2.

    Journal: Journal of Translational Medicine

    Article Title: Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission

    doi: 10.1186/1479-5876-9-151

    Figure Lengend Snippet: Activation of NK cells by DCs . Allogeneic and autologous non-adherent PBMCs were cocultured with DCs at a ratio of 10:1 for 24 h. Cells were stained for CD3, CD56, CD69 and IFN-γ expression and analyzed by flow cytometry. (A) Shown is the percentage of CD69 expression on CD3 - /CD56 + gated NK cells. Representative histograms of unstimulated NK cells, IL-2 (500 IU/mL) activated NK cells, DCs + autologous NK cells (upper panel) and DCs + allogeneic NK cells (lower panel) of one AML patient are shown (cocktail R was used for DC maturation). The dot blots below show the corresponding intracellular IFN-γ staining, with PMA/ionomycin serving as a positive control. (B) Summary of the percentage of CD69 expression on CD3 - /CD56 + NK cells after coculture with allogeneic and autologous NK cells from HC and AML patients. (C) Production of IFN-γ after coculture of DCs and isolated NK cells was assessed by ELISA. DCs were generated and cocultured with allogeneic NK cells at a ratio of 1:10 for 24 h. Supernatant was analyzed by standard ELISA for IFN-γ. The comparison of HC and AML patients is shown using the four different maturation cocktails. As a positive control NK cells were stimulated with IL-2.

    Article Snippet: Cell isolation and generation of mature DCs from PBMCs In brief, PBMCs were isolated by Ficoll/Hypaque (Biochrom, Berlin, Germany) density gradient centrifugation.

    Techniques: Activation Assay, Staining, Expressing, Flow Cytometry, Cytometry, Positive Control, Isolation, Enzyme-linked Immunosorbent Assay, Generated

    Efficient in vitro generation of P4 B*15 - and P1 B*07 -specific CD8 + T cells from naive T cells of CLL patients and HBDs. Representative tetramer stainings of CD8 + T cells after three cycles of aAPC-based in vitro priming using CD8 + T cells derived from HLA-matched HBDs primed with (A) the HLA-B*15-restricted peptide HQKRPIPIKY (P4 B*15 ) and (B) the HLA-B*07-restricted peptide RPIPIKYKAM (P1 B*07 ) as well as from HLA-matched MYD88 WT CLL patient (CLL-05) primed with (C) the HLA-B*07-restricted peptide RPIPIKYKAM (P1 B*07 ): 1st column: tetramer staining of CD8 + T cells primed with the MYD88 L265P -derived peptide; 2nd column: control staining with HLA-matched tetramer containing a non-relevant control peptide on CD8 + T cells derived from the same population as T cells depicted in the 1st column; 3rd column: ex vivo tetramer staining of CD8 + T cells. In vitro primings with HBD-derived PBMCs were performed in six (P1 B*07 ) and three (P4 B*15 ) independent replicates, respectively. For the in vitro priming with PBMCs of CLL patients two independent replicates were conducted. Abbreviations: neg., negative.

    Journal: Oncoimmunology

    Article Title: HLA class I-restricted MYD88 L265P-derived peptides as specific targets for lymphoma immunotherapy

    doi: 10.1080/2162402X.2016.1219825

    Figure Lengend Snippet: Efficient in vitro generation of P4 B*15 - and P1 B*07 -specific CD8 + T cells from naive T cells of CLL patients and HBDs. Representative tetramer stainings of CD8 + T cells after three cycles of aAPC-based in vitro priming using CD8 + T cells derived from HLA-matched HBDs primed with (A) the HLA-B*15-restricted peptide HQKRPIPIKY (P4 B*15 ) and (B) the HLA-B*07-restricted peptide RPIPIKYKAM (P1 B*07 ) as well as from HLA-matched MYD88 WT CLL patient (CLL-05) primed with (C) the HLA-B*07-restricted peptide RPIPIKYKAM (P1 B*07 ): 1st column: tetramer staining of CD8 + T cells primed with the MYD88 L265P -derived peptide; 2nd column: control staining with HLA-matched tetramer containing a non-relevant control peptide on CD8 + T cells derived from the same population as T cells depicted in the 1st column; 3rd column: ex vivo tetramer staining of CD8 + T cells. In vitro primings with HBD-derived PBMCs were performed in six (P1 B*07 ) and three (P4 B*15 ) independent replicates, respectively. For the in vitro priming with PBMCs of CLL patients two independent replicates were conducted. Abbreviations: neg., negative.

    Article Snippet: Peripheral blood mononuclear cells (PBMCs) from patients as well as PBMCs from healthy blood donors (HBDs) were isolated by density gradient centrifugation (Biocoll, Biochrom GmbH, L 6113).

    Techniques: In Vitro, Derivative Assay, Staining, Ex Vivo