mononuclear cells mncs  (Millipore)


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    Structured Review

    Millipore mononuclear cells mncs
    Ca 2+ /hypoxia-treated <t>hUCB-MSCs</t> maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of <t>MNCs</t> from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P
    Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 92 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells"

    Article Title: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

    Journal: Clinical and Translational Medicine

    doi: 10.1186/s40169-017-0168-z

    Ca 2+ /hypoxia-treated hUCB-MSCs maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of MNCs from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P
    Figure Legend Snippet: Ca 2+ /hypoxia-treated hUCB-MSCs maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of MNCs from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P

    Techniques Used: Isolation, Concentration Assay, Cell Counting

    2) Product Images from "Protective and Detrimental Roles for Regulatory T Cells in a Viral Model for Multiple Sclerosis"

    Article Title: Protective and Detrimental Roles for Regulatory T Cells in a Viral Model for Multiple Sclerosis

    Journal: Brain pathology (Zurich, Switzerland)

    doi: 10.1111/bpa.12119

    Cytokine responses to TMEV during the acute stage of infection. MNCs from the iTreg-early mice (open bars) produced more anti-inflammatory cytokine IL-10 ( A ) and IL-4 ( B ) and less inflammatory cytokine IFN-γ ( C ) and IL-17 ( D ) in response to ConA
    Figure Legend Snippet: Cytokine responses to TMEV during the acute stage of infection. MNCs from the iTreg-early mice (open bars) produced more anti-inflammatory cytokine IL-10 ( A ) and IL-4 ( B ) and less inflammatory cytokine IFN-γ ( C ) and IL-17 ( D ) in response to ConA

    Techniques Used: Infection, Mouse Assay, Produced

    Immune responses to TMEV during the acute stage of infection. A. MNCs were isolated from spleens of iTreg-early mice (open bars) or control mice (closed bars) on the peak of the acute stage and stimulated with TMEV at a MOI of 5, 1, 0.1 or by irradiated
    Figure Legend Snippet: Immune responses to TMEV during the acute stage of infection. A. MNCs were isolated from spleens of iTreg-early mice (open bars) or control mice (closed bars) on the peak of the acute stage and stimulated with TMEV at a MOI of 5, 1, 0.1 or by irradiated

    Techniques Used: Infection, Isolation, Mouse Assay, Irradiation

    Related Articles

    Centrifugation:

    Article Title: PD-L1 Expression on Circulating CD34+ Hematopoietic Stem Cells Closely Correlated with T-cell Apoptosis in Chronic Hepatitis C Infected Patients
    Article Snippet: .. Mononuclear cells (MNCs) were then isolated by density-gradient centrifugation of Ficoll 400 (Ficoll® Paque Plus, GE17-1440-02, Sigma-Aldrich, Germany) as previously described ( ). .. Then CD34+ cells were isolated by using CD34+ MiniMacs high-gradient magnetic separation column (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instruction.

    Gradient Centrifugation:

    Article Title: Simultaneous Measurement of Human Hematopoietic Stem and Progenitor Cells In Blood Using Multi-color Flow Cytometry
    Article Snippet: .. Mononuclear cells (MNCs) were purified by Histopaque 1077 (Sigma-Aldrich) gradient centrifugation. .. The MNC fraction was washed twice by centrifugation and resuspended in HBSS at room temperature to remove residual platelets.

    Article Title: CXCR7-dependent angiogenic mononuclear cell trafficking regulates tumor progression in multiple myeloma
    Article Snippet: .. Mononuclear cells (MNCs) from the BM and peripheral blood (PB) of MM patients and healthy subjects were obtained by Ficoll (Sigma-Aldrich, St. Louis, MO) gradient centrifugation, as previously described. .. Primary MM cells were obtained using CD138+ micro-bead selection (Miltenyi Biotec, Auburn, CA).

    Article Title: Decitabine enhances stem cell antigen-1 expression in cigarette smoke extract-induced emphysema in animal model
    Article Snippet: .. Mononuclear cells (MNCs) from bone marrow of C57BL/6J mice was isolated by Ficoll density gradient centrifugation (Histopaque-1083, Sigma), and then inoculated into culture flask with density of (3–5) × 106 /mL, cultured with endothelial growth medium-2 (EGM-2) in presence of 5% fetal bovine serum (SingleQuots®, Lonza, Switzerland) under an atmosphere of 95% humidity, 5% CO2 at 37℃. ..

    Isolation:

    Article Title: Hypoxia-Induced Endothelial Progenitor Cell Function Is Blunted in Angiotensinogen Knockout Mice
    Article Snippet: .. Mononuclear cells (MNCs) were isolated from WT or AGT+/− mouse bone marrow (BM) or peripheral blood (PB) using a Histopaque-1083 density gradient based method (Sigma, USA). .. Freshly isolated MNCs were resuspended in EGM-2 media (Lonza, Switzerland) supplemented with 5% fetal bovine serum (FBS, Hyclone, USA), basic fibroblast growth factor (bFGF), VEGF, insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), ascorbic acid and heparin, and seeded onto 60 mm dishes (2 × 107 cells/dish).

    Article Title: Protective and Detrimental Roles for Regulatory T Cells in a Viral Model for Multiple Sclerosis
    Article Snippet: .. Spleens were removed from TMEV-infected mice during the acute or chronic stage of disease, mononuclear cells (MNCs) were isolated using Histopaque® 1083 (Sigma-Aldrich) ( ). .. A volume of 100 μl of 2 × 105 MNCs in RPMI 1640 supplemented with 1% glutamine, 1% antibiotics, 50 μM 2-mercaptoethanol, and 10% FBS was added to each well of 96-well plates.

    Article Title: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells
    Article Snippet: .. To isolate and expand MSCs from cord blood, mononuclear cells (MNCs) were isolated using a Ficoll–Hypaque solution (d = 1.077 g/cm3 ; Sigma). ..

    Article Title: Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
    Article Snippet: .. The isolation of mononuclear cells (MNCs) was performed according to the method of Boyum [ ] modified using a Histopaque™ 1.077 density gradient (Sigma-Aldrich, São Paulo, Brazil). .. EPCs (CD133+ ) were selected using CD133-coupled magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer's instructions.

    Article Title: PD-L1 Expression on Circulating CD34+ Hematopoietic Stem Cells Closely Correlated with T-cell Apoptosis in Chronic Hepatitis C Infected Patients
    Article Snippet: .. Mononuclear cells (MNCs) were then isolated by density-gradient centrifugation of Ficoll 400 (Ficoll® Paque Plus, GE17-1440-02, Sigma-Aldrich, Germany) as previously described ( ). .. Then CD34+ cells were isolated by using CD34+ MiniMacs high-gradient magnetic separation column (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instruction.

    Article Title: Decitabine enhances stem cell antigen-1 expression in cigarette smoke extract-induced emphysema in animal model
    Article Snippet: .. Mononuclear cells (MNCs) from bone marrow of C57BL/6J mice was isolated by Ficoll density gradient centrifugation (Histopaque-1083, Sigma), and then inoculated into culture flask with density of (3–5) × 106 /mL, cultured with endothelial growth medium-2 (EGM-2) in presence of 5% fetal bovine serum (SingleQuots®, Lonza, Switzerland) under an atmosphere of 95% humidity, 5% CO2 at 37℃. ..

    Cell Culture:

    Article Title: Decitabine enhances stem cell antigen-1 expression in cigarette smoke extract-induced emphysema in animal model
    Article Snippet: .. Mononuclear cells (MNCs) from bone marrow of C57BL/6J mice was isolated by Ficoll density gradient centrifugation (Histopaque-1083, Sigma), and then inoculated into culture flask with density of (3–5) × 106 /mL, cultured with endothelial growth medium-2 (EGM-2) in presence of 5% fetal bovine serum (SingleQuots®, Lonza, Switzerland) under an atmosphere of 95% humidity, 5% CO2 at 37℃. ..

    Purification:

    Article Title: Simultaneous Measurement of Human Hematopoietic Stem and Progenitor Cells In Blood Using Multi-color Flow Cytometry
    Article Snippet: .. Mononuclear cells (MNCs) were purified by Histopaque 1077 (Sigma-Aldrich) gradient centrifugation. .. The MNC fraction was washed twice by centrifugation and resuspended in HBSS at room temperature to remove residual platelets.

    Modification:

    Article Title: Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
    Article Snippet: .. The isolation of mononuclear cells (MNCs) was performed according to the method of Boyum [ ] modified using a Histopaque™ 1.077 density gradient (Sigma-Aldrich, São Paulo, Brazil). .. EPCs (CD133+ ) were selected using CD133-coupled magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer's instructions.

    Mouse Assay:

    Article Title: Protective and Detrimental Roles for Regulatory T Cells in a Viral Model for Multiple Sclerosis
    Article Snippet: .. Spleens were removed from TMEV-infected mice during the acute or chronic stage of disease, mononuclear cells (MNCs) were isolated using Histopaque® 1083 (Sigma-Aldrich) ( ). .. A volume of 100 μl of 2 × 105 MNCs in RPMI 1640 supplemented with 1% glutamine, 1% antibiotics, 50 μM 2-mercaptoethanol, and 10% FBS was added to each well of 96-well plates.

    Article Title: Decitabine enhances stem cell antigen-1 expression in cigarette smoke extract-induced emphysema in animal model
    Article Snippet: .. Mononuclear cells (MNCs) from bone marrow of C57BL/6J mice was isolated by Ficoll density gradient centrifugation (Histopaque-1083, Sigma), and then inoculated into culture flask with density of (3–5) × 106 /mL, cultured with endothelial growth medium-2 (EGM-2) in presence of 5% fetal bovine serum (SingleQuots®, Lonza, Switzerland) under an atmosphere of 95% humidity, 5% CO2 at 37℃. ..

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    Millipore culture pbmcs
    Features of ssRNA40-induced LILRA3 expression. a Kinetics of LILRA3 expression was compared to other ssRNA40 inducible cytokines. <t>PBMCs</t> from two donors were stimulated with ssRNA40 or ssRNA41 and the RNA was harvested at various time points. qPCR for LILRA3 , TNF , IFNb , IFNg , and IL1b expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (pooled cDNA from 10. b Effect of cytokine inhibition on LILRA3 expression. PBMCs from two donors were stimulated overnight with ssRNA40 together with neutralizing agents anakinra, certolizumab, tocilizumab and IFN-γ neutralizing antibodies and analysed for LILRA3 and IL6 expression by qPCR. Repeated measures ANOVA was used to calculate difference of ssRNA40+ neutralization to ssRNA40 alone and results displayed as mean ± SD
    Culture Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore pbmc specimens against sd1 lps
    Fecal s-IgA antibody titers to <t>SD1-LPS</t> in group1 (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol) monkeys, expressed as MN with MN ± SE, after challenge on day 00 and re-challenge on day 31 with SD11617 strain.
    Pbmc Specimens Against Sd1 Lps, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore human pbmcs
    Imatinib inhibits TNF-α production in human myeloid cells. Human <t>PBMCs,</t> monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml <t>LPS.</t> ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.
    Human Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/Millipore
    Average 93 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
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    94
    Millipore host pbmcs
    Effects of the various concentrations of <t>rHcMTF-12</t> on <t>PBMC</t> migration in vitro . Cells were treated with control buffer, pET32a protein and multiple concentrations of rHcMTF-12. The migration percentage was determined randomly. The difference between the mean values was calculated using ANOVA. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. Data are representative of three independent experiments. * P
    Host Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/host pbmcs/product/Millipore
    Average 94 stars, based on 1 article reviews
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    Features of ssRNA40-induced LILRA3 expression. a Kinetics of LILRA3 expression was compared to other ssRNA40 inducible cytokines. PBMCs from two donors were stimulated with ssRNA40 or ssRNA41 and the RNA was harvested at various time points. qPCR for LILRA3 , TNF , IFNb , IFNg , and IL1b expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (pooled cDNA from 10. b Effect of cytokine inhibition on LILRA3 expression. PBMCs from two donors were stimulated overnight with ssRNA40 together with neutralizing agents anakinra, certolizumab, tocilizumab and IFN-γ neutralizing antibodies and analysed for LILRA3 and IL6 expression by qPCR. Repeated measures ANOVA was used to calculate difference of ssRNA40+ neutralization to ssRNA40 alone and results displayed as mean ± SD

    Journal: Retrovirology

    Article Title: TLR8 regulation of LILRA3 in monocytes is abrogated in human immunodeficiency virus infection and correlates to CD4 counts and virus loads

    doi: 10.1186/s12977-016-0248-y

    Figure Lengend Snippet: Features of ssRNA40-induced LILRA3 expression. a Kinetics of LILRA3 expression was compared to other ssRNA40 inducible cytokines. PBMCs from two donors were stimulated with ssRNA40 or ssRNA41 and the RNA was harvested at various time points. qPCR for LILRA3 , TNF , IFNb , IFNg , and IL1b expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (pooled cDNA from 10. b Effect of cytokine inhibition on LILRA3 expression. PBMCs from two donors were stimulated overnight with ssRNA40 together with neutralizing agents anakinra, certolizumab, tocilizumab and IFN-γ neutralizing antibodies and analysed for LILRA3 and IL6 expression by qPCR. Repeated measures ANOVA was used to calculate difference of ssRNA40+ neutralization to ssRNA40 alone and results displayed as mean ± SD

    Article Snippet: PBMC and monocyte isolation and culture PBMCs were isolated using density gradient centrifugation by layering blood onto Biocoll (Millipore) and centrifugation at 1200×g for 20 min without brake.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Inhibition, Neutralization

    LILRA3 induction by TLRs. a Induction of LILRA3 and IL6 expression by a panel of TLR agonists. LILRA3 and IL6 expression was measured, using qPCR, as fold change to the unstimulated control from PBMCs stimulated for 24 h with Pam3CSK4 (P3C), heat killed Listeria monocytogenes (HKLM), polyinosinic-polycytidylic acid (Poly I:C) in high molecular weight (HMW) and low molecular weight (LMW) forms, lipopolysaccharide (LPS), flagellin, synthetic diacylated lipoprotein FSL-1, Imiquimod (R837), ssRNA40/LyoVec and CpG oligonucleotide ODN 2006. b Induction of LILRA3 by ssRNA40 on the transcriptional level. LILRA3 expression was measured as fold change to the unstimulated control from PBMCs stimulated for 24 h with uridine-rich HIV-derived ssRNA40, using ssRNA41 as control (n = 8). Wilcoxon matched-pairs signed rank test was used to compare the median between ssRNA41 and ssRNA40 stimulated LILRA3 expression. Scatter dot plot overlayed on bar graph displayed as median with interquartile range. c Induction of LILRA3 in PBMCS by ssRNA40 on the protein level. 30 × 10 6 /mL PBMCs from LILRA3 + / + and LILRA3 − / − donors were stimulated with 5 μg/mL ssRNA40. 40 µL from each supernatant was loaded and Ponceau S staining was used as loading control. The membrane was blotted for LILRA3 and IL6, using recombinant LILRA3 as protein control. d Expression of ssRNA40-induced LILRA3 by monocytes. PBMCs stimulated overnight with ssRNA40 and LPS were purified for CD14 + monocytes using magnetic-activated cell sorting. The resulting CD14 + positive and depleted fractions were analysed via qPCR for LILRA3 expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (*p

    Journal: Retrovirology

    Article Title: TLR8 regulation of LILRA3 in monocytes is abrogated in human immunodeficiency virus infection and correlates to CD4 counts and virus loads

    doi: 10.1186/s12977-016-0248-y

    Figure Lengend Snippet: LILRA3 induction by TLRs. a Induction of LILRA3 and IL6 expression by a panel of TLR agonists. LILRA3 and IL6 expression was measured, using qPCR, as fold change to the unstimulated control from PBMCs stimulated for 24 h with Pam3CSK4 (P3C), heat killed Listeria monocytogenes (HKLM), polyinosinic-polycytidylic acid (Poly I:C) in high molecular weight (HMW) and low molecular weight (LMW) forms, lipopolysaccharide (LPS), flagellin, synthetic diacylated lipoprotein FSL-1, Imiquimod (R837), ssRNA40/LyoVec and CpG oligonucleotide ODN 2006. b Induction of LILRA3 by ssRNA40 on the transcriptional level. LILRA3 expression was measured as fold change to the unstimulated control from PBMCs stimulated for 24 h with uridine-rich HIV-derived ssRNA40, using ssRNA41 as control (n = 8). Wilcoxon matched-pairs signed rank test was used to compare the median between ssRNA41 and ssRNA40 stimulated LILRA3 expression. Scatter dot plot overlayed on bar graph displayed as median with interquartile range. c Induction of LILRA3 in PBMCS by ssRNA40 on the protein level. 30 × 10 6 /mL PBMCs from LILRA3 + / + and LILRA3 − / − donors were stimulated with 5 μg/mL ssRNA40. 40 µL from each supernatant was loaded and Ponceau S staining was used as loading control. The membrane was blotted for LILRA3 and IL6, using recombinant LILRA3 as protein control. d Expression of ssRNA40-induced LILRA3 by monocytes. PBMCs stimulated overnight with ssRNA40 and LPS were purified for CD14 + monocytes using magnetic-activated cell sorting. The resulting CD14 + positive and depleted fractions were analysed via qPCR for LILRA3 expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (*p

    Article Snippet: PBMC and monocyte isolation and culture PBMCs were isolated using density gradient centrifugation by layering blood onto Biocoll (Millipore) and centrifugation at 1200×g for 20 min without brake.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Molecular Weight, Derivative Assay, Staining, Recombinant, Purification, FACS

    LILRA3 stimulation of cytokines and antigen presenting cells. a qPCR of LILRA3 induced gene expression of LILRA3 , IL - 6 , IL - 1A , IL - 1B and IL - 10 . Due to the different optimal concentrations of LILRA3 for various donors, the gene expression of all four donors were shown, with the corresponding mean ± SD. b Effect of LILRA3 on the antigen presentation mechanism of monocytes and B-cells. PBMCs were stimulated with varying concentrations of LILRA3 for 48 h and analysed by flow cytometry for expression of CD80, CD86, HLA-DR and HLA-ABC on monocytes (CD14 + CD33 + ) and B-cells (CD3 + CD19 + ). On monocytes, upregulation of HLA-ABC and CD80 was observed, whereas CD86 was downregulated. On B-cells, there was a slight but significant upregulation of HLA-DR and CD86. 1-way ANOVA with repeated measures Dunett post test was used to compare to 0 ng/mL LILRA3 control. Results expressed as mean ± SD from six donors. (*p

    Journal: Retrovirology

    Article Title: TLR8 regulation of LILRA3 in monocytes is abrogated in human immunodeficiency virus infection and correlates to CD4 counts and virus loads

    doi: 10.1186/s12977-016-0248-y

    Figure Lengend Snippet: LILRA3 stimulation of cytokines and antigen presenting cells. a qPCR of LILRA3 induced gene expression of LILRA3 , IL - 6 , IL - 1A , IL - 1B and IL - 10 . Due to the different optimal concentrations of LILRA3 for various donors, the gene expression of all four donors were shown, with the corresponding mean ± SD. b Effect of LILRA3 on the antigen presentation mechanism of monocytes and B-cells. PBMCs were stimulated with varying concentrations of LILRA3 for 48 h and analysed by flow cytometry for expression of CD80, CD86, HLA-DR and HLA-ABC on monocytes (CD14 + CD33 + ) and B-cells (CD3 + CD19 + ). On monocytes, upregulation of HLA-ABC and CD80 was observed, whereas CD86 was downregulated. On B-cells, there was a slight but significant upregulation of HLA-DR and CD86. 1-way ANOVA with repeated measures Dunett post test was used to compare to 0 ng/mL LILRA3 control. Results expressed as mean ± SD from six donors. (*p

    Article Snippet: PBMC and monocyte isolation and culture PBMCs were isolated using density gradient centrifugation by layering blood onto Biocoll (Millipore) and centrifugation at 1200×g for 20 min without brake.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Flow Cytometry, Cytometry

    Fecal s-IgA antibody titers to SD1-LPS in group1 (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol) monkeys, expressed as MN with MN ± SE, after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Journal: Apmis

    Article Title: Evaluation of an intragastric challenge model for Shigella dysenteriae 1 in rhesus monkeys (Macaca mulatta) for the pre-clinical assessment of Shigella vaccine formulations

    doi: 10.1111/apm.12168

    Figure Lengend Snippet: Fecal s-IgA antibody titers to SD1-LPS in group1 (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol) monkeys, expressed as MN with MN ± SE, after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Article Snippet: Antibody-secreting cell assay Enzyme-linked immunospot (ELISPOT) assay was performed to enumerate IgA, IgG, and IgM ASCs on fresh PBMC specimens against SD1 LPS and SD1 Invaplex antigens using Multiscreen Immobilon-P filtration, 96-well plates, EMD Millipore Corporation, Billerica, MA, USA .

    Techniques:

    IgA, IgG, and IgM antibody titers against SD1-LPS in group 1 monkeys (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol), expressed as geometric mean titer (GMT) with GMT±SE after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Journal: Apmis

    Article Title: Evaluation of an intragastric challenge model for Shigella dysenteriae 1 in rhesus monkeys (Macaca mulatta) for the pre-clinical assessment of Shigella vaccine formulations

    doi: 10.1111/apm.12168

    Figure Lengend Snippet: IgA, IgG, and IgM antibody titers against SD1-LPS in group 1 monkeys (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol), expressed as geometric mean titer (GMT) with GMT±SE after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Article Snippet: Antibody-secreting cell assay Enzyme-linked immunospot (ELISPOT) assay was performed to enumerate IgA, IgG, and IgM ASCs on fresh PBMC specimens against SD1 LPS and SD1 Invaplex antigens using Multiscreen Immobilon-P filtration, 96-well plates, EMD Millipore Corporation, Billerica, MA, USA .

    Techniques:

    Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The kinase inhibitor imatinib mesylate inhibits TNF-? production in vitro and prevents TNF-dependent acute hepatic inflammation

    doi: 10.1073/pnas.0501758102

    Figure Lengend Snippet: Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Article Snippet: Human PBMCs or CD14-selected monocytes were stimulated with LPS at a concentration of 100 ng/ml for 12 h in the presence of either solvent or the indicated kinase inhibitor ( , SB203580, PD98059, and JNK-Inhibitor II, all used in a final concentration of 35 μM and purchased from Calbiochem).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing

    Effects of the various concentrations of rHcMTF-12 on PBMC migration in vitro . Cells were treated with control buffer, pET32a protein and multiple concentrations of rHcMTF-12. The migration percentage was determined randomly. The difference between the mean values was calculated using ANOVA. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. Data are representative of three independent experiments. * P

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Effects of the various concentrations of rHcMTF-12 on PBMC migration in vitro . Cells were treated with control buffer, pET32a protein and multiple concentrations of rHcMTF-12. The migration percentage was determined randomly. The difference between the mean values was calculated using ANOVA. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. Data are representative of three independent experiments. * P

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: Migration, In Vitro, Derivative Assay

    Relative expression of multiple cytokines in goat PBMCs stimulated by rHcMTF-12. Cells were incubated with rHcMTF-12 for 48 h, and the mRNAs encoding IL-2, IL-4, IL-6, IL-10, TGF-β1 and IFN-γ were quantified by real-time PCR. The significant level was set at * P

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Relative expression of multiple cytokines in goat PBMCs stimulated by rHcMTF-12. Cells were incubated with rHcMTF-12 for 48 h, and the mRNAs encoding IL-2, IL-4, IL-6, IL-10, TGF-β1 and IFN-γ were quantified by real-time PCR. The significant level was set at * P

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction

    Decreased level of cell proliferation by rHcMTF-12 in vitro . Cells were simulated with ConA and treated with control buffer (PBS), pET32a protein and various concentrations of rHcMTF-12 protein for 72 h. A proliferation test was conducted by CCK-8 and values were measured at 450 nm wavelength (OD 450 ) using microplate spectrophotometer. Cell proliferation index was calculated by considering the OD 450 values in the controls as 100%. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. The data are expressed as the mean ± standard error (SE) of three independent experiments. ** P

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Decreased level of cell proliferation by rHcMTF-12 in vitro . Cells were simulated with ConA and treated with control buffer (PBS), pET32a protein and various concentrations of rHcMTF-12 protein for 72 h. A proliferation test was conducted by CCK-8 and values were measured at 450 nm wavelength (OD 450 ) using microplate spectrophotometer. Cell proliferation index was calculated by considering the OD 450 values in the controls as 100%. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. The data are expressed as the mean ± standard error (SE) of three independent experiments. ** P

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: In Vitro, CCK-8 Assay, Spectrophotometry, Derivative Assay

    Binding of rHcMTF-12 protein with goat PBMCs. The cells were incubated with rat sera anti-rHcMTF-12 IgG, negative rat IgG or anti-pET32a protein IgG as the primary antibody followed by Cy3-labeled goat anti-rat IgG as the secondary antibody. Red fluorescence on surface of cells showed target protein staining (Cy3) and cell nuclei were visualized by DAPI (blue). No fluorescence was observed in the PBS control or pET32 control groups. Scale-bars : 10 μm

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Binding of rHcMTF-12 protein with goat PBMCs. The cells were incubated with rat sera anti-rHcMTF-12 IgG, negative rat IgG or anti-pET32a protein IgG as the primary antibody followed by Cy3-labeled goat anti-rat IgG as the secondary antibody. Red fluorescence on surface of cells showed target protein staining (Cy3) and cell nuclei were visualized by DAPI (blue). No fluorescence was observed in the PBS control or pET32 control groups. Scale-bars : 10 μm

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: Binding Assay, Incubation, Labeling, Fluorescence, Staining