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Ficoll-Paque Pharmacia mononuclear cells mncs
Mononuclear Cells Mncs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mononuclear cells mncs/product/Ficoll-Paque Pharmacia
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mononuclear cells mncs - by Bioz Stars, 2021-03
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Isolation:

Article Title: Dose-Dependent Circulating Immunoglobulin A Antibody-Secreting Cell and Serum Antibody Responses in Swedish Volunteers to an Oral Inactivated Enterotoxigenic Escherichia coli Vaccine
Article Snippet: .. Mononuclear cells (MNCs) from heparinized venous blood were isolated by standard gradient centrifugation on Ficoll-Paque (Pharmacia Biotech AB, Uppsala, Sweden). .. The numbers of IgA-secreting cells and antigen-specific IgA ASCs were measured by a micromodification of the original enzyme-linked immunospot assay ( , ).

Article Title: Clonal expansion is a characteristic feature of the B-cell repertoire of patients with rheumatoid arthritis
Article Snippet: .. Mononuclear cells (MNCs) were isolated from cell suspensions from blood, synovial fluid, and synovial tissue by density gradient centrifugation (Ficoll-Paque; Pharmacia LKB Biotechnology, Piscataway, NJ, USA). .. Isolation of DNA and RNA, and preparation of complementary DNA Genomic DNA was isolated from MNCs using the Puregene DNA Isolation Kit (Gentra Systems, Mineapolis, MN, USA) and total RNA was isolated using Ultraspec RNA (Biotech Laboratories, Houston, TX, USA).

Article Title: Chemical, physical and biological triggers of evolutionary conserved Bcl-xL-mediated apoptosis
Article Snippet: .. Mononuclear cells (MNCs) were isolated by density gradient separation (Ficoll-Paque PREMIUM; GE Healthcare, Little Chalfont, Buckinghamshire, UK) and immediately analyzed or vitally frozen for later use. .. Cells were maintained in mononuclear cell medium (MCM; Promocell, Heidelberg, Germany) or in a 25% HS-5 CM plus 75% RPMI 1640 medium mix.

Article Title: Preclinical platform for long-term evaluation of immuno-oncology drugs using hCD34+ humanized mouse model
Article Snippet: .. Mononuclear cells (MNCs) were separated from hUCB using Ficoll-Paque Plus density gradient centrifugation. hCD34+ cells were isolated from MNCs using a magnetic activated cell sorting (MACS) human CD34 MicroBead Kit (Miltenyi Biotec, Spain) according to the manufacturer’s instructions. .. The purity of hCD34+ cells was determined using a CytoFLEX flow cytometer.

Article Title: miR-34b Targets HSF1 to Suppress Cell Survival in Acute Myeloid Leukemia
Article Snippet: Cell Culture The human leukemia cell line HL-60 was purchased from American Type Cell Culture Collection (Manassas, VA, USA) and OCI-AML3 from DSMZ (Braunschweig, Germany) and deemed free of mycoplasma and bacterial contaminants. .. Primary AML blasts or mononuclear cells (MNCs) from peripheral blood samples of healthy donors were isolated by density-gradient centrifugation using the Ficoll-Paque Plus kits (Ficoll, Piscataway, NJ, USA). .. Blood cells with specific surface markers were further purified from MNCs of healthy donors by cell sorting with antibodies (APC-CD11b, PE-CD14).

Article Title: Endothelial progenitor cells with stem cells enhance osteogenic efficacy
Article Snippet: MSCs were isolated according to the method previously reported [ ]. .. Mononuclear cells (MNCs) were isolated from all heparinized BM aspirates by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare, Uppsala, Sweden). .. The MNCs were then washed in PBS and cultured in culture flasks (Corning, New York, USA) with α-MEM (HyClone, Logan, USA) containing 10% (V/V) fetal bovine serum (GIBCO, Carlsbad, USA).

Gradient Centrifugation:

Article Title: Dose-Dependent Circulating Immunoglobulin A Antibody-Secreting Cell and Serum Antibody Responses in Swedish Volunteers to an Oral Inactivated Enterotoxigenic Escherichia coli Vaccine
Article Snippet: .. Mononuclear cells (MNCs) from heparinized venous blood were isolated by standard gradient centrifugation on Ficoll-Paque (Pharmacia Biotech AB, Uppsala, Sweden). .. The numbers of IgA-secreting cells and antigen-specific IgA ASCs were measured by a micromodification of the original enzyme-linked immunospot assay ( , ).

Article Title: Clonal expansion is a characteristic feature of the B-cell repertoire of patients with rheumatoid arthritis
Article Snippet: .. Mononuclear cells (MNCs) were isolated from cell suspensions from blood, synovial fluid, and synovial tissue by density gradient centrifugation (Ficoll-Paque; Pharmacia LKB Biotechnology, Piscataway, NJ, USA). .. Isolation of DNA and RNA, and preparation of complementary DNA Genomic DNA was isolated from MNCs using the Puregene DNA Isolation Kit (Gentra Systems, Mineapolis, MN, USA) and total RNA was isolated using Ultraspec RNA (Biotech Laboratories, Houston, TX, USA).

Article Title: Preclinical platform for long-term evaluation of immuno-oncology drugs using hCD34+ humanized mouse model
Article Snippet: .. Mononuclear cells (MNCs) were separated from hUCB using Ficoll-Paque Plus density gradient centrifugation. hCD34+ cells were isolated from MNCs using a magnetic activated cell sorting (MACS) human CD34 MicroBead Kit (Miltenyi Biotec, Spain) according to the manufacturer’s instructions. .. The purity of hCD34+ cells was determined using a CytoFLEX flow cytometer.

Article Title: Endothelial progenitor cells with stem cells enhance osteogenic efficacy
Article Snippet: MSCs were isolated according to the method previously reported [ ]. .. Mononuclear cells (MNCs) were isolated from all heparinized BM aspirates by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare, Uppsala, Sweden). .. The MNCs were then washed in PBS and cultured in culture flasks (Corning, New York, USA) with α-MEM (HyClone, Logan, USA) containing 10% (V/V) fetal bovine serum (GIBCO, Carlsbad, USA).

FACS:

Article Title: Preclinical platform for long-term evaluation of immuno-oncology drugs using hCD34+ humanized mouse model
Article Snippet: .. Mononuclear cells (MNCs) were separated from hUCB using Ficoll-Paque Plus density gradient centrifugation. hCD34+ cells were isolated from MNCs using a magnetic activated cell sorting (MACS) human CD34 MicroBead Kit (Miltenyi Biotec, Spain) according to the manufacturer’s instructions. .. The purity of hCD34+ cells was determined using a CytoFLEX flow cytometer.

Magnetic Cell Separation:

Article Title: Preclinical platform for long-term evaluation of immuno-oncology drugs using hCD34+ humanized mouse model
Article Snippet: .. Mononuclear cells (MNCs) were separated from hUCB using Ficoll-Paque Plus density gradient centrifugation. hCD34+ cells were isolated from MNCs using a magnetic activated cell sorting (MACS) human CD34 MicroBead Kit (Miltenyi Biotec, Spain) according to the manufacturer’s instructions. .. The purity of hCD34+ cells was determined using a CytoFLEX flow cytometer.

Centrifugation:

Article Title: miR-34b Targets HSF1 to Suppress Cell Survival in Acute Myeloid Leukemia
Article Snippet: Cell Culture The human leukemia cell line HL-60 was purchased from American Type Cell Culture Collection (Manassas, VA, USA) and OCI-AML3 from DSMZ (Braunschweig, Germany) and deemed free of mycoplasma and bacterial contaminants. .. Primary AML blasts or mononuclear cells (MNCs) from peripheral blood samples of healthy donors were isolated by density-gradient centrifugation using the Ficoll-Paque Plus kits (Ficoll, Piscataway, NJ, USA). .. Blood cells with specific surface markers were further purified from MNCs of healthy donors by cell sorting with antibodies (APC-CD11b, PE-CD14).

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    Ficoll-Paque Pharmacia pbmcs
    Semiquantitative <t>PCR</t> analysis to evaluate differences in proviral load. (A) PCR to detect BLV pol in <t>PBMC</t> from treated rabbits. Rabbit PBMC were harvested at 1, 4, and 10 months postinoculation and subjected to PCR with BLV pol primers KB560 and KB561 (10 × 10 4 PBMC) or β- actin to control for sample variation (2 × 10 4 PBMC). Each panel is labeled with month of sample harvest. Lanes are labeled with the source of PBMC DNA by rabbit number and treatment, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). In lower panels, the corresponding rabbit samples are designated by the matching parallel black lines. Positions of BLV pol amplicon (591 bp) and β- actin amplicon (594 bp) are designated. *, not determined (ND) because sample was not available; †, not determined because the animal died before harvest. (B) PCR standard curve. PBMC lysate harvested at 1 month postinoculation from BLV rabbit 44-10 was serially diluted in a range of 5 × 10 2 to 5 × 10 4 PBMC and subjected to PCR with BLV pol primers KB560 and KB561. Each lane is labeled with the number of cells used for PCR amplification, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). The arrow on the left indicates the position of BLV pol amplicon (591 bp), and the lines on the right indicate positions of 676- and 571-bp DNA size markers.
    Pbmcs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Ficoll-Paque Pharmacia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Ficoll-Paque Pharmacia pbmc preparation method
    Trima-associated gene expression signatures (A) Identification of gene expression patterns across all <t>PBMC</t> cell-types (left) linked to method of PBMC isolation (e.g., <t>Ficoll</t> or Trima; right). n = 15,340 cells. (B) Identification of Trima-specific marker genes in classical monocytes (left) and NK cells (right). n = 2,303 classical monocytes, n = 1,545 NK cells. Marker gene expression values are depicted as log1p-normalized counts.
    Pbmc Preparation Method, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc preparation method/product/Ficoll-Paque Pharmacia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc preparation method - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Ficoll-Paque Pharmacia hla class i typing peripheral blood mononuclear cells pbmcs
    <t>HLA-HIV</t> associations in Mesoamerican cohorts using 5 HIV clinical parameters (univariable analysis). Associations between the expression of HLA <t>class</t> I alleles and 5 HIV clinical parameters (pVL, CD4 count, Z-score, CD4% and CD4/CD8 ratio) were investigated for alleles with frequency equal or greater than 5 in HIV-1 clade B-infected ART-naïve individuals from the pooled MEX/CAM cohort ( A ), only in MEX cohort ( B ) or only in CAM cohort ( C ). Associations were evaluated using the Mann-Whitney U test and multiple tests were addressed using q-values. Boxplots of only significant (p
    Hla Class I Typing Peripheral Blood Mononuclear Cells Pbmcs, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla class i typing peripheral blood mononuclear cells pbmcs/product/Ficoll-Paque Pharmacia
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hla class i typing peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Semiquantitative PCR analysis to evaluate differences in proviral load. (A) PCR to detect BLV pol in PBMC from treated rabbits. Rabbit PBMC were harvested at 1, 4, and 10 months postinoculation and subjected to PCR with BLV pol primers KB560 and KB561 (10 × 10 4 PBMC) or β- actin to control for sample variation (2 × 10 4 PBMC). Each panel is labeled with month of sample harvest. Lanes are labeled with the source of PBMC DNA by rabbit number and treatment, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). In lower panels, the corresponding rabbit samples are designated by the matching parallel black lines. Positions of BLV pol amplicon (591 bp) and β- actin amplicon (594 bp) are designated. *, not determined (ND) because sample was not available; †, not determined because the animal died before harvest. (B) PCR standard curve. PBMC lysate harvested at 1 month postinoculation from BLV rabbit 44-10 was serially diluted in a range of 5 × 10 2 to 5 × 10 4 PBMC and subjected to PCR with BLV pol primers KB560 and KB561. Each lane is labeled with the number of cells used for PCR amplification, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). The arrow on the left indicates the position of BLV pol amplicon (591 bp), and the lines on the right indicate positions of 676- and 571-bp DNA size markers.

    Journal: Journal of Virology

    Article Title: Bovine Leukemia Virus Structural Gene Vectors Are Immunogenic and Lack Pathogenicity in a Rabbit Model

    doi:

    Figure Lengend Snippet: Semiquantitative PCR analysis to evaluate differences in proviral load. (A) PCR to detect BLV pol in PBMC from treated rabbits. Rabbit PBMC were harvested at 1, 4, and 10 months postinoculation and subjected to PCR with BLV pol primers KB560 and KB561 (10 × 10 4 PBMC) or β- actin to control for sample variation (2 × 10 4 PBMC). Each panel is labeled with month of sample harvest. Lanes are labeled with the source of PBMC DNA by rabbit number and treatment, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). In lower panels, the corresponding rabbit samples are designated by the matching parallel black lines. Positions of BLV pol amplicon (591 bp) and β- actin amplicon (594 bp) are designated. *, not determined (ND) because sample was not available; †, not determined because the animal died before harvest. (B) PCR standard curve. PBMC lysate harvested at 1 month postinoculation from BLV rabbit 44-10 was serially diluted in a range of 5 × 10 2 to 5 × 10 4 PBMC and subjected to PCR with BLV pol primers KB560 and KB561. Each lane is labeled with the number of cells used for PCR amplification, FLK(BLV) (positive control DNA [100 ng] from BLV-producing fetal lamb kidney cells), or marker (pGem DNA size standard [Promega]). The arrow on the left indicates the position of BLV pol amplicon (591 bp), and the lines on the right indicate positions of 676- and 571-bp DNA size markers.

    Article Snippet: To prepare PBMCs samples for PCR, PBMCs were isolated by Ficoll-Paque (Pharmacia) gradient centrifugation and washed three times with phosphate-buffered saline.

    Techniques: Polymerase Chain Reaction, Labeling, Positive Control, Marker, Amplification

    Trima-associated gene expression signatures (A) Identification of gene expression patterns across all PBMC cell-types (left) linked to method of PBMC isolation (e.g., Ficoll or Trima; right). n = 15,340 cells. (B) Identification of Trima-specific marker genes in classical monocytes (left) and NK cells (right). n = 2,303 classical monocytes, n = 1,545 NK cells. Marker gene expression values are depicted as log1p-normalized counts.

    Journal: bioRxiv

    Article Title: No detectable alloreactive transcriptional responses during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

    doi: 10.1101/2020.02.12.946509

    Figure Lengend Snippet: Trima-associated gene expression signatures (A) Identification of gene expression patterns across all PBMC cell-types (left) linked to method of PBMC isolation (e.g., Ficoll or Trima; right). n = 15,340 cells. (B) Identification of Trima-specific marker genes in classical monocytes (left) and NK cells (right). n = 2,303 classical monocytes, n = 1,545 NK cells. Marker gene expression values are depicted as log1p-normalized counts.

    Article Snippet: Moreover, our results demonstrate how PBMC preparation method (e.g., Ficoll-Paque density gradient centrifugation with or without Trima filtration) and sample multiplexing technology (e.g., SCMK) can introduce confounding variables into scRNA-seq data.

    Techniques: Expressing, Isolation, Marker

    Exploring alloreactivity in CD4+ T-cell subset and classical monocytes (A) Marker genes used for CD4+ T-cell subtype annotations (left) in Ficoll PBMCs. n = 6,879 CD4+ T-cells. (B) Evenly-subsetted classical monocyte gene expression space grouped according to donor ID (e.g., A, B, C) and mixing status (e.g., - = unmixed, + = mixed). n = 384 classical monocytes. (C) Quantitative inter-sample comparison using Jensen-Shannon Divergence (JSD) and hierarchical clustering demonstrates similarity between evenly-subsetted classical monocytes from distinct healthy donors regardless of mixing. (D) Expression of genes known to be up-regulated (e.g., IFNG and CD40LG) or down-regulated (e.g., DUSP1 and FOS) in lymphocytes during an allogenic response.

    Journal: bioRxiv

    Article Title: No detectable alloreactive transcriptional responses during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

    doi: 10.1101/2020.02.12.946509

    Figure Lengend Snippet: Exploring alloreactivity in CD4+ T-cell subset and classical monocytes (A) Marker genes used for CD4+ T-cell subtype annotations (left) in Ficoll PBMCs. n = 6,879 CD4+ T-cells. (B) Evenly-subsetted classical monocyte gene expression space grouped according to donor ID (e.g., A, B, C) and mixing status (e.g., - = unmixed, + = mixed). n = 384 classical monocytes. (C) Quantitative inter-sample comparison using Jensen-Shannon Divergence (JSD) and hierarchical clustering demonstrates similarity between evenly-subsetted classical monocytes from distinct healthy donors regardless of mixing. (D) Expression of genes known to be up-regulated (e.g., IFNG and CD40LG) or down-regulated (e.g., DUSP1 and FOS) in lymphocytes during an allogenic response.

    Article Snippet: Moreover, our results demonstrate how PBMC preparation method (e.g., Ficoll-Paque density gradient centrifugation with or without Trima filtration) and sample multiplexing technology (e.g., SCMK) can introduce confounding variables into scRNA-seq data.

    Techniques: Marker, Expressing

    HLA-HIV associations in Mesoamerican cohorts using 5 HIV clinical parameters (univariable analysis). Associations between the expression of HLA class I alleles and 5 HIV clinical parameters (pVL, CD4 count, Z-score, CD4% and CD4/CD8 ratio) were investigated for alleles with frequency equal or greater than 5 in HIV-1 clade B-infected ART-naïve individuals from the pooled MEX/CAM cohort ( A ), only in MEX cohort ( B ) or only in CAM cohort ( C ). Associations were evaluated using the Mann-Whitney U test and multiple tests were addressed using q-values. Boxplots of only significant (p

    Journal: Scientific Reports

    Article Title: Novel HLA class I associations with HIV-1 control in a unique genetically admixed population

    doi: 10.1038/s41598-018-23849-7

    Figure Lengend Snippet: HLA-HIV associations in Mesoamerican cohorts using 5 HIV clinical parameters (univariable analysis). Associations between the expression of HLA class I alleles and 5 HIV clinical parameters (pVL, CD4 count, Z-score, CD4% and CD4/CD8 ratio) were investigated for alleles with frequency equal or greater than 5 in HIV-1 clade B-infected ART-naïve individuals from the pooled MEX/CAM cohort ( A ), only in MEX cohort ( B ) or only in CAM cohort ( C ). Associations were evaluated using the Mann-Whitney U test and multiple tests were addressed using q-values. Boxplots of only significant (p

    Article Snippet: HLA class I typing Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Pharmacia, Uppsala, SE) from blood samples from MEX cohort, while buffy coats were isolated from CAM cohort blood samples and cryopreserved until DNA extraction.

    Techniques: Expressing, Infection, Chick Chorioallantoic Membrane Assay, MANN-WHITNEY

    HLA class I haplotype structures and linkage disequilibrium in the MEX (panel A) and CAM (panel B) cohorts. HLA loci are stacked vertically, with each orange tile representing a specific HLA subtype, and with segments connecting linked alleles on adjacent loci. The height of each tile and the thickness of each segment correspond to HLA allele and haplotype frequencies, respectively. The most frequent HLA allele pairs (two-loci) found to be in linkage disequilibrium are highlighted in green (PF > 0.10) and blue (PF

    Journal: Scientific Reports

    Article Title: Novel HLA class I associations with HIV-1 control in a unique genetically admixed population

    doi: 10.1038/s41598-018-23849-7

    Figure Lengend Snippet: HLA class I haplotype structures and linkage disequilibrium in the MEX (panel A) and CAM (panel B) cohorts. HLA loci are stacked vertically, with each orange tile representing a specific HLA subtype, and with segments connecting linked alleles on adjacent loci. The height of each tile and the thickness of each segment correspond to HLA allele and haplotype frequencies, respectively. The most frequent HLA allele pairs (two-loci) found to be in linkage disequilibrium are highlighted in green (PF > 0.10) and blue (PF

    Article Snippet: HLA class I typing Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Pharmacia, Uppsala, SE) from blood samples from MEX cohort, while buffy coats were isolated from CAM cohort blood samples and cryopreserved until DNA extraction.

    Techniques: Chick Chorioallantoic Membrane Assay

    Comparison of HLA class I allele frequencies between the Mestizo MEX/CAM cohort (n = 3213) and the mainly Caucasian HOMER cohort (n = 1622). Allele frequencies (2n) were calculated using the HLA Analysis tool from Los Alamos HIV Database ( https://www.hiv.lanl.gov ); all HLA AF > 0.001 in at least one cohort are shown here. AF were compared using Fisher’s exact test, with multiple tests addressed using q-values 42 . Significant differences (p

    Journal: Scientific Reports

    Article Title: Novel HLA class I associations with HIV-1 control in a unique genetically admixed population

    doi: 10.1038/s41598-018-23849-7

    Figure Lengend Snippet: Comparison of HLA class I allele frequencies between the Mestizo MEX/CAM cohort (n = 3213) and the mainly Caucasian HOMER cohort (n = 1622). Allele frequencies (2n) were calculated using the HLA Analysis tool from Los Alamos HIV Database ( https://www.hiv.lanl.gov ); all HLA AF > 0.001 in at least one cohort are shown here. AF were compared using Fisher’s exact test, with multiple tests addressed using q-values 42 . Significant differences (p

    Article Snippet: HLA class I typing Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque Pharmacia, Uppsala, SE) from blood samples from MEX cohort, while buffy coats were isolated from CAM cohort blood samples and cryopreserved until DNA extraction.

    Techniques: Chick Chorioallantoic Membrane Assay