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Baxter Healthcare mononuclear cells mncs
CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
Mononuclear Cells Mncs, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb"

Article Title: Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb

Journal: Circulation research

doi: 10.1161/CIRCRESAHA.116.310557

CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
Figure Legend Snippet: CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

Techniques Used: Protein Array, Expressing

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    Baxter Healthcare mononuclear cells mncs
    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing <t>CD34</t> + cells with <t>MNCs</t> and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).
    Mononuclear Cells Mncs, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Baxter Healthcare
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Baxter Healthcare pbmcs pbmc
    Identification of CMV pp65 15-mer and nanomer epitopes from peptide subpool 8 that were reactive with CD8+ T cells . Testing of IE-1 15-mers from subpool 8 against cells from donor 2 (gold bar), donor 5 (red bar), donor 6 (light blue bar), and donor 7 (navy blue bar) revealed that the peptide IE-1 297–311 was reactive with donor 6, IE-1 305–319 was reactive with donor 7, IE-1 309–323 was reactive with donors 2 and 6, and IE-1 313–327 was reactive with donor 5 (Panel A). Testing of the 6 nanomers overlapping IE-1 297–311 against cells from donor 6 revealed that IE-1 300–308 was the dominant nanomer (Panel B). Testing of the nanomers spanning the overlapping 15-mers IE-1 305–319 , IE-1 309–323 , and IE-1 313–327 against <t>PBMCs</t> from donors 2, 5, and 7 identified 4 nanomers that were reactive with CD8+ T cells: IE-1 305–313 , IE-1 308–316 , IE-1 312–320 and IE-1 319–327 , (Panel C).
    Pbmcs Pbmc, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs pbmc/product/Baxter Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmcs pbmc - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Baxter Healthcare hla a 0201 positive pbmc
    Identification of CMV pp65 15-mer and nanomer epitopes from peptide subpool 8 that were reactive with CD8+ T cells . Testing of IE-1 15-mers from subpool 8 against cells from donor 2 (gold bar), donor 5 (red bar), donor 6 (light blue bar), and donor 7 (navy blue bar) revealed that the peptide IE-1 297–311 was reactive with donor 6, IE-1 305–319 was reactive with donor 7, IE-1 309–323 was reactive with donors 2 and 6, and IE-1 313–327 was reactive with donor 5 (Panel A). Testing of the 6 nanomers overlapping IE-1 297–311 against cells from donor 6 revealed that IE-1 300–308 was the dominant nanomer (Panel B). Testing of the nanomers spanning the overlapping 15-mers IE-1 305–319 , IE-1 309–323 , and IE-1 313–327 against <t>PBMCs</t> from donors 2, 5, and 7 identified 4 nanomers that were reactive with CD8+ T cells: IE-1 305–313 , IE-1 308–316 , IE-1 312–320 and IE-1 319–327 , (Panel C).
    Hla A 0201 Positive Pbmc, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla a 0201 positive pbmc/product/Baxter Healthcare
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hla a 0201 positive pbmc - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    92
    Baxter Healthcare pbmcs
    Identification of CMV pp65 15-mer and nanomer epitopes from peptide subpool 8 that were reactive with CD8+ T cells . Testing of IE-1 15-mers from subpool 8 against cells from donor 2 (gold bar), donor 5 (red bar), donor 6 (light blue bar), and donor 7 (navy blue bar) revealed that the peptide IE-1 297–311 was reactive with donor 6, IE-1 305–319 was reactive with donor 7, IE-1 309–323 was reactive with donors 2 and 6, and IE-1 313–327 was reactive with donor 5 (Panel A). Testing of the 6 nanomers overlapping IE-1 297–311 against cells from donor 6 revealed that IE-1 300–308 was the dominant nanomer (Panel B). Testing of the nanomers spanning the overlapping 15-mers IE-1 305–319 , IE-1 309–323 , and IE-1 313–327 against <t>PBMCs</t> from donors 2, 5, and 7 identified 4 nanomers that were reactive with CD8+ T cells: IE-1 305–313 , IE-1 308–316 , IE-1 312–320 and IE-1 319–327 , (Panel C).
    Pbmcs, supplied by Baxter Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Baxter Healthcare
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

    Journal: Circulation research

    Article Title: Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb

    doi: 10.1161/CIRCRESAHA.116.310557

    Figure Lengend Snippet: CD34Exo carry pro-angiogenic miRNA. Human angiogenesis protein array comparing CD34Exo and MNCExo ( A ); quantification of proteins present in CD34Exo and MNCExo using human angiogenesis protein array ( B ); miRNA expression profiles comparing CD34 + cells with MNCs and CD34Exo with MNCExo, shown using heat map expression analysis ( C ).

    Article Snippet: CD34+ cells and the CD34+ -cell–depleted mononuclear cells (MNCs) were obtained from Baxter Healthcare Corporation (Deerfield, IL, USA).

    Techniques: Protein Array, Expressing

    Identification of CMV pp65 15-mer and nanomer epitopes from peptide subpool 8 that were reactive with CD8+ T cells . Testing of IE-1 15-mers from subpool 8 against cells from donor 2 (gold bar), donor 5 (red bar), donor 6 (light blue bar), and donor 7 (navy blue bar) revealed that the peptide IE-1 297–311 was reactive with donor 6, IE-1 305–319 was reactive with donor 7, IE-1 309–323 was reactive with donors 2 and 6, and IE-1 313–327 was reactive with donor 5 (Panel A). Testing of the 6 nanomers overlapping IE-1 297–311 against cells from donor 6 revealed that IE-1 300–308 was the dominant nanomer (Panel B). Testing of the nanomers spanning the overlapping 15-mers IE-1 305–319 , IE-1 309–323 , and IE-1 313–327 against PBMCs from donors 2, 5, and 7 identified 4 nanomers that were reactive with CD8+ T cells: IE-1 305–313 , IE-1 308–316 , IE-1 312–320 and IE-1 319–327 , (Panel C).

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV pp65 15-mer and nanomer epitopes from peptide subpool 8 that were reactive with CD8+ T cells . Testing of IE-1 15-mers from subpool 8 against cells from donor 2 (gold bar), donor 5 (red bar), donor 6 (light blue bar), and donor 7 (navy blue bar) revealed that the peptide IE-1 297–311 was reactive with donor 6, IE-1 305–319 was reactive with donor 7, IE-1 309–323 was reactive with donors 2 and 6, and IE-1 313–327 was reactive with donor 5 (Panel A). Testing of the 6 nanomers overlapping IE-1 297–311 against cells from donor 6 revealed that IE-1 300–308 was the dominant nanomer (Panel B). Testing of the nanomers spanning the overlapping 15-mers IE-1 305–319 , IE-1 309–323 , and IE-1 313–327 against PBMCs from donors 2, 5, and 7 identified 4 nanomers that were reactive with CD8+ T cells: IE-1 305–313 , IE-1 308–316 , IE-1 312–320 and IE-1 319–327 , (Panel C).

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques:

    Identification of CMV pp65 15-mer epitopes from peptide subpool 13 that were reactive with CD4+ T cells . PBMCs from 5 donors were reactive with pp65 peptides in subpool 13. Testing of all 15-mers in subpool 13 identified three 15-mers that were reactive with CD4+ T cells, pp65 489–503 , pp65 505–519 , and pp65 509–523 .

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV pp65 15-mer epitopes from peptide subpool 13 that were reactive with CD4+ T cells . PBMCs from 5 donors were reactive with pp65 peptides in subpool 13. Testing of all 15-mers in subpool 13 identified three 15-mers that were reactive with CD4+ T cells, pp65 489–503 , pp65 505–519 , and pp65 509–523 .

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques:

    Identification of CMV IE-1 epitopes using 15-mer and nanomer peptides . PBMCs were stimulated with a library of 120 15-mer peptides overlapping by 11 residues and covering the entire IE-1 protein. IFN-γ flow cytometry was used to detect T cell responses. To identify specific epitopes, PBMCs were stimulated with peptide subpools containing 10 overlapping 15-mer peptides. PBMCs from donors with reactive CD8+ T cells were tested further with individual 15-mers and nanomers. The results of analysis of CD8+ T cell responses in donor 6 to IE-1 peptides are shown.

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV IE-1 epitopes using 15-mer and nanomer peptides . PBMCs were stimulated with a library of 120 15-mer peptides overlapping by 11 residues and covering the entire IE-1 protein. IFN-γ flow cytometry was used to detect T cell responses. To identify specific epitopes, PBMCs were stimulated with peptide subpools containing 10 overlapping 15-mer peptides. PBMCs from donors with reactive CD8+ T cells were tested further with individual 15-mers and nanomers. The results of analysis of CD8+ T cell responses in donor 6 to IE-1 peptides are shown.

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques: Flow Cytometry, Cytometry

    Identification of CMV pp65 15-mer and nanomer epitopes recognized by CD8+ T cells . PBMCs from CMV-seropositive subjects reactive with peptides in a pp65 subpool were tested against all the individual 15-mers in each subpool. Following the identification of reactive 15-mers, nanomers overlapping at 8 amino acids and spanning the reactive 15-mer peptides were tested against PBMCs from the reactive subject. The reactive 15-mer and nanomer peptides are shown. Testing of cells from 7 subjects led to the identification of 6 pp65 15-mers reactive with CD8+ T cells. Testing of the overlapping nanomers identified 5 epitopes. Cells from donor 3 were not available to test with the nanomer peptides. Although CD8+ T cells from donor 10 were reactive with peptides in subpool 6 and a reactive 15-mer, pp65 221–235 , was identified, no reactive nanomer was identified. NT = not tested.

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV pp65 15-mer and nanomer epitopes recognized by CD8+ T cells . PBMCs from CMV-seropositive subjects reactive with peptides in a pp65 subpool were tested against all the individual 15-mers in each subpool. Following the identification of reactive 15-mers, nanomers overlapping at 8 amino acids and spanning the reactive 15-mer peptides were tested against PBMCs from the reactive subject. The reactive 15-mer and nanomer peptides are shown. Testing of cells from 7 subjects led to the identification of 6 pp65 15-mers reactive with CD8+ T cells. Testing of the overlapping nanomers identified 5 epitopes. Cells from donor 3 were not available to test with the nanomer peptides. Although CD8+ T cells from donor 10 were reactive with peptides in subpool 6 and a reactive 15-mer, pp65 221–235 , was identified, no reactive nanomer was identified. NT = not tested.

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques:

    Identification of CMV IE-1 15-mer and nanomer epitopes from 3 peptide subpools reactive with CD8+ T cells . PBMCs from 4 donors were reactive with peptides in IE-1 subpools 3, 5, and 10. Testing of all 15-mers in each subpool identified 3 nanomers reactive wi th CD8+ T cells, one from each subpool. Testing of the 6 overlapping nanomers spanning each reactive 15-mer lead to the identification of 3 nanomer epitopes.

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV IE-1 15-mer and nanomer epitopes from 3 peptide subpools reactive with CD8+ T cells . PBMCs from 4 donors were reactive with peptides in IE-1 subpools 3, 5, and 10. Testing of all 15-mers in each subpool identified 3 nanomers reactive wi th CD8+ T cells, one from each subpool. Testing of the 6 overlapping nanomers spanning each reactive 15-mer lead to the identification of 3 nanomer epitopes.

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques:

    Identification of CMV pp65 15-mer epitopes from 3 peptide subpools that were reactive with CD4+ T cells . PBMCs from 8 donors were reactive with peptides in subpools 6, 8, and 10. Testing of all 15-mers in each subpool identified three15-mers that were reactive with CD4+ T cells, one from each subpool.

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV pp65 15-mer epitopes from 3 peptide subpools that were reactive with CD4+ T cells . PBMCs from 8 donors were reactive with peptides in subpools 6, 8, and 10. Testing of all 15-mers in each subpool identified three15-mers that were reactive with CD4+ T cells, one from each subpool.

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques:

    Identification of CMV pp65 epitopes using 15-mer and nanomer peptides . PBMCs were stimulated with a library of 138 15-mer peptides overlapping by 11 residues and covering the entire pp65 protein. IFN-γ flow cytometry was used to detect T cell responses. To identify specific epitopes, PBMCs were stimulated with subpools containing 10 overlapping 15-mer peptides. PBMCs from donors reactive with CD4+ T cells were tested further with individual 15-mers. The results of analysis of CD4+ T cell responses in donor 14 to pp65 peptides are shown.

    Journal: Journal of Translational Medicine

    Article Title: CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects

    doi: 10.1186/1479-5876-5-17

    Figure Lengend Snippet: Identification of CMV pp65 epitopes using 15-mer and nanomer peptides . PBMCs were stimulated with a library of 138 15-mer peptides overlapping by 11 residues and covering the entire pp65 protein. IFN-γ flow cytometry was used to detect T cell responses. To identify specific epitopes, PBMCs were stimulated with subpools containing 10 overlapping 15-mer peptides. PBMCs from donors reactive with CD4+ T cells were tested further with individual 15-mers. The results of analysis of CD4+ T cell responses in donor 14 to pp65 peptides are shown.

    Article Snippet: Collection of PBMCs PBMC concentrates from 20 CMV-seropositive and 5 CMV-seronegative healthy donors were collected by apheresis, (Fenwal CS-3000 Plus, Baxter Healthcare Corp, Deerfield, IL) and peripheral blood mononuclear cells were isolated by ficoll-hypaque density gradient separation and frozen in aliquots of 1 × 108 cells.

    Techniques: Flow Cytometry, Cytometry