Structured Review

Axis-Shield Diagnostics mononuclear cells mncs
Chemical inhibition of ABCC6 increases the efficacy of dasatinib but not imatinib in patient <t>MNCs.</t> p-CRKL determined IC50 was determined via incubating patient MNCs with increasing concentrations of (A) dasatinib and (B) imatinib in the absence and presence of three ABCC6 inhibitors: pantoprazole, indomethacin, probenecid. CRKL western blotting was performed to determine the concentration of <t>TKI</t> required for 50% Bcr-Abl kinase inhibition (IC50). Representative western blots are shown in S3 and S4 Figs. The box plots depict the median, the upper 25th and the lower 75th percentiles while the whiskers encompass the 10th and 90th percentiles. Statistical analyses were performed using Student’s t -test with statistically p -values denoted by asterisks (** p
Mononuclear Cells Mncs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "ABCC6 plays a significant role in the transport of nilotinib and dasatinib, and contributes to TKI resistance in vitro, in both cell lines and primary patient mononuclear cells"

Article Title: ABCC6 plays a significant role in the transport of nilotinib and dasatinib, and contributes to TKI resistance in vitro, in both cell lines and primary patient mononuclear cells

Journal: PLoS ONE

doi: 10.1371/journal.pone.0192180

Chemical inhibition of ABCC6 increases the efficacy of dasatinib but not imatinib in patient MNCs. p-CRKL determined IC50 was determined via incubating patient MNCs with increasing concentrations of (A) dasatinib and (B) imatinib in the absence and presence of three ABCC6 inhibitors: pantoprazole, indomethacin, probenecid. CRKL western blotting was performed to determine the concentration of TKI required for 50% Bcr-Abl kinase inhibition (IC50). Representative western blots are shown in S3 and S4 Figs. The box plots depict the median, the upper 25th and the lower 75th percentiles while the whiskers encompass the 10th and 90th percentiles. Statistical analyses were performed using Student’s t -test with statistically p -values denoted by asterisks (** p
Figure Legend Snippet: Chemical inhibition of ABCC6 increases the efficacy of dasatinib but not imatinib in patient MNCs. p-CRKL determined IC50 was determined via incubating patient MNCs with increasing concentrations of (A) dasatinib and (B) imatinib in the absence and presence of three ABCC6 inhibitors: pantoprazole, indomethacin, probenecid. CRKL western blotting was performed to determine the concentration of TKI required for 50% Bcr-Abl kinase inhibition (IC50). Representative western blots are shown in S3 and S4 Figs. The box plots depict the median, the upper 25th and the lower 75th percentiles while the whiskers encompass the 10th and 90th percentiles. Statistical analyses were performed using Student’s t -test with statistically p -values denoted by asterisks (** p

Techniques Used: Inhibition, Western Blot, Concentration Assay

2) Product Images from "Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming"

Article Title: Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming

Journal: Journal of Clinical Medicine

doi: 10.3390/jcm9040968

Frequency of MSC from different donor cohorts. Cohort specific: ( A ) percentage of donors yielding at least one CFU-F; ( B ) CFU-F/100 ml blood; ( C ) CFU-F/10 9 MNCs. Error bars are standard deviations.
Figure Legend Snippet: Frequency of MSC from different donor cohorts. Cohort specific: ( A ) percentage of donors yielding at least one CFU-F; ( B ) CFU-F/100 ml blood; ( C ) CFU-F/10 9 MNCs. Error bars are standard deviations.

Techniques Used:

Related Articles

Centrifugation:

Article Title: Soluble Guanylate Cyclase Agonists Inhibit Expression and Procoagulant Activity of Tissue Factor
Article Snippet: .. Mononuclear cells (MNCs) were obtained by density centrifugation using a Lymphoprep solution (Axis-Shield). .. We also studied the potential of both sGC agonists to inhibit TF activation in human vascular endothelium in a model of resting or TNF-α–stimulated HUVECs (ATCC, Manassas, VA).

Article Title: MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
Article Snippet: .. The obtained cell suspension was filtered through a 70-μm cell strainer (BD Biosciences, San Jose, CA, USA) and washed in phosphate-buffered saline (PBS) by centrifugation at 400×g for 5 min. For flow cytometry, mononuclear cells (MNCs) were isolated by density gradient centrifugation at 900× g for 20 min using Ficoll-Hypaque (Axis-Shield, Dundee, Scotland). .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque density gradient centrifugation and either allowed to rest overnight in complete medium before use in the functional assay (see below) or cryopreserved in liquid nitrogen until required for the phenotypic analysis (see below).

In Vitro:

Article Title: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase
Article Snippet: .. Blood leucocytes were isolated after lysis of erythrocytes in heparinized blood by incubation with ammonium chloride (In Vitro As, Fredensborg, Denmark) for 7 min. Mononuclear cells (MNCs) were isolated by gradient centrifugation of heparinized blood using LymphoPrep™ (Axis-Shield, Oslo, Norway). .. Before use, both cell preparations were washed twice in RPMI 1640, 25 mM Hepes containing 0.42 mM calcium nitrate, l -glutamine and gentamicin (In Vitro As).

Isolation:

Article Title: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase
Article Snippet: .. Blood leucocytes were isolated after lysis of erythrocytes in heparinized blood by incubation with ammonium chloride (In Vitro As, Fredensborg, Denmark) for 7 min. Mononuclear cells (MNCs) were isolated by gradient centrifugation of heparinized blood using LymphoPrep™ (Axis-Shield, Oslo, Norway). .. Before use, both cell preparations were washed twice in RPMI 1640, 25 mM Hepes containing 0.42 mM calcium nitrate, l -glutamine and gentamicin (In Vitro As).

Article Title: Determination of complex subclonal structures of hematological malignancies by multiplexed genotyping of blood progenitor colonies
Article Snippet: .. As described previously , mononuclear cells (MNCs) were isolated from 40 mL of peripheral blood using a sodium diatrizoate/polysaccharide density gradient (Lymphoprep; Axis Shield PLC, Oslo, Norway) according to the manufacturer's instructions. .. MNCs were plated at a density of 1 × 106 cells/mL in MethoCult H4034 (StemCell Technologies, Vancouver, Canada).

Article Title: Different Isolation Methods Alter the Gene Expression Profiling of Adipose Derived Stem Cells
Article Snippet: .. Mononuclear cells (MNCs) were isolated by layering onto a lymphoprep density gradient (1:2; Axis-Shield PoCAS). ..

Article Title: Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming
Article Snippet: .. Preparation of Tissue and Generation of MSC Cultures Since plating of whole blood can lead to clot formation, mononuclear cells (MNCs) were isolated from whole blood and BM using Lymphoprep (Axis-Shield, Dundee, UK) density gradient centrifugation, accepting that some MSCs could theoretically be missed. ..

Article Title: MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
Article Snippet: .. The obtained cell suspension was filtered through a 70-μm cell strainer (BD Biosciences, San Jose, CA, USA) and washed in phosphate-buffered saline (PBS) by centrifugation at 400×g for 5 min. For flow cytometry, mononuclear cells (MNCs) were isolated by density gradient centrifugation at 900× g for 20 min using Ficoll-Hypaque (Axis-Shield, Dundee, Scotland). .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque density gradient centrifugation and either allowed to rest overnight in complete medium before use in the functional assay (see below) or cryopreserved in liquid nitrogen until required for the phenotypic analysis (see below).

Article Title: ABCC6 plays a significant role in the transport of nilotinib and dasatinib, and contributes to TKI resistance in vitro, in both cell lines and primary patient mononuclear cells
Article Snippet: .. Patient cells Peripheral blood was obtained from de novo chronic phase CML patients before commencement of TKI therapy and mononuclear cells (MNCs) were isolated using Lymphoprep (Axis Shield, Oslo, Norway) density gradient centrifugation. .. TKIs and efflux transporter inhibitors Imatinib mesylate (Glivec® ) and nilotinib (Tasigna® ) were provided by Novartis Pharmaceuticals (Basel, Switzerland), dasatinib (Sprycel® ) was provided by Bristol-Myers Squibb (Victoria, Australia).

Cytometry:

Article Title: MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
Article Snippet: .. The obtained cell suspension was filtered through a 70-μm cell strainer (BD Biosciences, San Jose, CA, USA) and washed in phosphate-buffered saline (PBS) by centrifugation at 400×g for 5 min. For flow cytometry, mononuclear cells (MNCs) were isolated by density gradient centrifugation at 900× g for 20 min using Ficoll-Hypaque (Axis-Shield, Dundee, Scotland). .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque density gradient centrifugation and either allowed to rest overnight in complete medium before use in the functional assay (see below) or cryopreserved in liquid nitrogen until required for the phenotypic analysis (see below).

Purification:

Article Title: RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia.
Article Snippet: .. N < sup > 6 < /sup > -methyladenosine (m < sup > 6 < /sup > A), the most abundant internal modification in mRNA, has been implicated in tumorigenesis. .. N < sup > 6 < /sup > -methyladenosine (m < sup > 6 < /sup > A), the most abundant internal modification in mRNA, has been implicated in tumorigenesis.

Incubation:

Article Title: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase
Article Snippet: .. Blood leucocytes were isolated after lysis of erythrocytes in heparinized blood by incubation with ammonium chloride (In Vitro As, Fredensborg, Denmark) for 7 min. Mononuclear cells (MNCs) were isolated by gradient centrifugation of heparinized blood using LymphoPrep™ (Axis-Shield, Oslo, Norway). .. Before use, both cell preparations were washed twice in RPMI 1640, 25 mM Hepes containing 0.42 mM calcium nitrate, l -glutamine and gentamicin (In Vitro As).

Lysis:

Article Title: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase
Article Snippet: .. Blood leucocytes were isolated after lysis of erythrocytes in heparinized blood by incubation with ammonium chloride (In Vitro As, Fredensborg, Denmark) for 7 min. Mononuclear cells (MNCs) were isolated by gradient centrifugation of heparinized blood using LymphoPrep™ (Axis-Shield, Oslo, Norway). .. Before use, both cell preparations were washed twice in RPMI 1640, 25 mM Hepes containing 0.42 mM calcium nitrate, l -glutamine and gentamicin (In Vitro As).

Planar Chromatography:

Article Title: Determination of complex subclonal structures of hematological malignancies by multiplexed genotyping of blood progenitor colonies
Article Snippet: .. As described previously , mononuclear cells (MNCs) were isolated from 40 mL of peripheral blood using a sodium diatrizoate/polysaccharide density gradient (Lymphoprep; Axis Shield PLC, Oslo, Norway) according to the manufacturer's instructions. .. MNCs were plated at a density of 1 × 106 cells/mL in MethoCult H4034 (StemCell Technologies, Vancouver, Canada).

Gradient Centrifugation:

Article Title: Reduced glutathione as a physiological co-activator in the activation of peptidylarginine deiminase
Article Snippet: .. Blood leucocytes were isolated after lysis of erythrocytes in heparinized blood by incubation with ammonium chloride (In Vitro As, Fredensborg, Denmark) for 7 min. Mononuclear cells (MNCs) were isolated by gradient centrifugation of heparinized blood using LymphoPrep™ (Axis-Shield, Oslo, Norway). .. Before use, both cell preparations were washed twice in RPMI 1640, 25 mM Hepes containing 0.42 mM calcium nitrate, l -glutamine and gentamicin (In Vitro As).

Article Title: Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming
Article Snippet: .. Preparation of Tissue and Generation of MSC Cultures Since plating of whole blood can lead to clot formation, mononuclear cells (MNCs) were isolated from whole blood and BM using Lymphoprep (Axis-Shield, Dundee, UK) density gradient centrifugation, accepting that some MSCs could theoretically be missed. ..

Article Title: MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
Article Snippet: .. The obtained cell suspension was filtered through a 70-μm cell strainer (BD Biosciences, San Jose, CA, USA) and washed in phosphate-buffered saline (PBS) by centrifugation at 400×g for 5 min. For flow cytometry, mononuclear cells (MNCs) were isolated by density gradient centrifugation at 900× g for 20 min using Ficoll-Hypaque (Axis-Shield, Dundee, Scotland). .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque density gradient centrifugation and either allowed to rest overnight in complete medium before use in the functional assay (see below) or cryopreserved in liquid nitrogen until required for the phenotypic analysis (see below).

Article Title: ABCC6 plays a significant role in the transport of nilotinib and dasatinib, and contributes to TKI resistance in vitro, in both cell lines and primary patient mononuclear cells
Article Snippet: .. Patient cells Peripheral blood was obtained from de novo chronic phase CML patients before commencement of TKI therapy and mononuclear cells (MNCs) were isolated using Lymphoprep (Axis Shield, Oslo, Norway) density gradient centrifugation. .. TKIs and efflux transporter inhibitors Imatinib mesylate (Glivec® ) and nilotinib (Tasigna® ) were provided by Novartis Pharmaceuticals (Basel, Switzerland), dasatinib (Sprycel® ) was provided by Bristol-Myers Squibb (Victoria, Australia).

Flow Cytometry:

Article Title: MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
Article Snippet: .. The obtained cell suspension was filtered through a 70-μm cell strainer (BD Biosciences, San Jose, CA, USA) and washed in phosphate-buffered saline (PBS) by centrifugation at 400×g for 5 min. For flow cytometry, mononuclear cells (MNCs) were isolated by density gradient centrifugation at 900× g for 20 min using Ficoll-Hypaque (Axis-Shield, Dundee, Scotland). .. Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque density gradient centrifugation and either allowed to rest overnight in complete medium before use in the functional assay (see below) or cryopreserved in liquid nitrogen until required for the phenotypic analysis (see below).

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    Axis-Shield Diagnostics pbmc s
    A) Levels of integrated proviral DNA in MDM and resting <t>PBMC</t> (pre-)treated with NAMPT (200 ng/ml) and infected with HIV BaL at 0.1 and 0.001 MOI, normalised to ERV-3, as assessed by RT-qPCR; B) viral binding to MDM, as quantified by p24 concentrations in cell lysates after 2 hours incubation and washing of the unbound virus; C) expression of CCR5 and CXCR4 on MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by flow cytometry [ 39 ]; D) secretion of the β-chemokines MIP1α, MIP1β and RANTES by MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by CBA. MFI: mean fluorescence intensity .
    Pbmc S, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 89/100, based on 358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc s/product/Axis-Shield Diagnostics
    Average 89 stars, based on 358 article reviews
    Price from $9.99 to $1999.99
    pbmc s - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    92
    Axis-Shield Diagnostics mononuclear cells mncs
    Chemical inhibition of ABCC6 increases the efficacy of dasatinib but not imatinib in patient <t>MNCs.</t> p-CRKL determined IC50 was determined via incubating patient MNCs with increasing concentrations of (A) dasatinib and (B) imatinib in the absence and presence of three ABCC6 inhibitors: pantoprazole, indomethacin, probenecid. CRKL western blotting was performed to determine the concentration of <t>TKI</t> required for 50% Bcr-Abl kinase inhibition (IC50). Representative western blots are shown in S3 and S4 Figs. The box plots depict the median, the upper 25th and the lower 75th percentiles while the whiskers encompass the 10th and 90th percentiles. Statistical analyses were performed using Student’s t -test with statistically p -values denoted by asterisks (** p
    Mononuclear Cells Mncs, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Axis-Shield Diagnostics
    Average 92 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    A) Levels of integrated proviral DNA in MDM and resting PBMC (pre-)treated with NAMPT (200 ng/ml) and infected with HIV BaL at 0.1 and 0.001 MOI, normalised to ERV-3, as assessed by RT-qPCR; B) viral binding to MDM, as quantified by p24 concentrations in cell lysates after 2 hours incubation and washing of the unbound virus; C) expression of CCR5 and CXCR4 on MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by flow cytometry [ 39 ]; D) secretion of the β-chemokines MIP1α, MIP1β and RANTES by MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by CBA. MFI: mean fluorescence intensity .

    Journal: Retrovirology

    Article Title: Transcriptome analysis of monocyte-HIV interactions

    doi: 10.1186/1742-4690-7-53

    Figure Lengend Snippet: A) Levels of integrated proviral DNA in MDM and resting PBMC (pre-)treated with NAMPT (200 ng/ml) and infected with HIV BaL at 0.1 and 0.001 MOI, normalised to ERV-3, as assessed by RT-qPCR; B) viral binding to MDM, as quantified by p24 concentrations in cell lysates after 2 hours incubation and washing of the unbound virus; C) expression of CCR5 and CXCR4 on MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by flow cytometry [ 39 ]; D) secretion of the β-chemokines MIP1α, MIP1β and RANTES by MDM treated 2 days with NAMPT (200 ng/ml) and LPS (100 ng/ml), as assessed by CBA. MFI: mean fluorescence intensity .

    Article Snippet: In vitro infection experiments For in vitro infection experiments, PBMC's were separated by Lymphoprep (Axis Shield, Dundee, United Kingdom) gradient from buffy coats of healthy donors of the Blood Transfusion Centre of Antwerp and were either employed as such in PBMC infection experiments or were used for monocyte preparation.

    Techniques: Infection, Quantitative RT-PCR, Binding Assay, Incubation, Expressing, Flow Cytometry, Cytometry, Crocin Bleaching Assay, Fluorescence

    Modulation of viral infectivity of the lab-attenuated strain HIV BaL by the secreted factors CCL2, NAMPT and PDGFC: infection of A) PBMC and B) MDM (pre-)treated with recombinant factors by HIV BaL . C) Modulation of the viral infectivity of the biological clones HIV 968-2 and HIV 968-3 by the secreted factor NAMPT in PBMC and MDM. TCID50 values were determined using the method of Reed Muench[ 36 ], based on p24 measurement in culture supernatants. Infectivity in treated cells is expressed as a percentage of infectivity in untreated control cells. Results in 3 independent donors are shown.

    Journal: Retrovirology

    Article Title: Transcriptome analysis of monocyte-HIV interactions

    doi: 10.1186/1742-4690-7-53

    Figure Lengend Snippet: Modulation of viral infectivity of the lab-attenuated strain HIV BaL by the secreted factors CCL2, NAMPT and PDGFC: infection of A) PBMC and B) MDM (pre-)treated with recombinant factors by HIV BaL . C) Modulation of the viral infectivity of the biological clones HIV 968-2 and HIV 968-3 by the secreted factor NAMPT in PBMC and MDM. TCID50 values were determined using the method of Reed Muench[ 36 ], based on p24 measurement in culture supernatants. Infectivity in treated cells is expressed as a percentage of infectivity in untreated control cells. Results in 3 independent donors are shown.

    Article Snippet: In vitro infection experiments For in vitro infection experiments, PBMC's were separated by Lymphoprep (Axis Shield, Dundee, United Kingdom) gradient from buffy coats of healthy donors of the Blood Transfusion Centre of Antwerp and were either employed as such in PBMC infection experiments or were used for monocyte preparation.

    Techniques: Infection, Recombinant, Clone Assay

    The TCR repertoires of Tregs and Tcons show high exclusivity in blood and SF of JIA patients. PBMCs and SFMCs taken at the same time from JIA patients were enriched for CD4 + T cells and then stained for CD4, CD127, and CD25. ( A ) Left panels show CD25 versus CD127 staining before sorting. Tregs (CD4 + CD127 lo CD25 hi ) and Tcons (CD4 + CD127 hi CD25 lo ) were sorted from both anatomical compartments; sort purities based on CD127 and CD25 expression are shown in the middle panels . A small sample of sorted cells was stained for FOXP3 ( right panels ). Genomic DNA was extracted from purified T cells and analyzed using the immunoSEQ TCRB survey platform (Adaptive Biotechnologies). ( B ) Graphs depict the clonality ( top panel ) and frequency of the most abundant clone ( bottom panel ) in the TCR repertoires of the four sorted subsets ( n = 4 individual JIA patients). Bars represent mean ± SEM. Statistical testing by one-way ANOVA. * p

    Journal: The Journal of Immunology Author Choice

    Article Title: Synovial Regulatory T Cells Occupy a Discrete TCR Niche in Human Arthritis and Require Local Signals To Stabilize FOXP3 Protein Expression

    doi: 10.4049/jimmunol.1500391

    Figure Lengend Snippet: The TCR repertoires of Tregs and Tcons show high exclusivity in blood and SF of JIA patients. PBMCs and SFMCs taken at the same time from JIA patients were enriched for CD4 + T cells and then stained for CD4, CD127, and CD25. ( A ) Left panels show CD25 versus CD127 staining before sorting. Tregs (CD4 + CD127 lo CD25 hi ) and Tcons (CD4 + CD127 hi CD25 lo ) were sorted from both anatomical compartments; sort purities based on CD127 and CD25 expression are shown in the middle panels . A small sample of sorted cells was stained for FOXP3 ( right panels ). Genomic DNA was extracted from purified T cells and analyzed using the immunoSEQ TCRB survey platform (Adaptive Biotechnologies). ( B ) Graphs depict the clonality ( top panel ) and frequency of the most abundant clone ( bottom panel ) in the TCR repertoires of the four sorted subsets ( n = 4 individual JIA patients). Bars represent mean ± SEM. Statistical testing by one-way ANOVA. * p

    Article Snippet: SFMCs and PBMCs were prepared by density gradient centrifugation using Lymphoprep (Axis-Shield) with SF samples undergoing pretreatment with hyaluronidase (10 U/ml; Sigma).

    Techniques: Staining, Expressing, Purification

    Comparison of HBV-specific cell responses in the presence or absence of HBeAg. (A) C protein-specific T-cell responses elicited in ELISPOT assay; (B) Cytokine IFN-Y responses in cultured supernatants of PBMCs stimulated by C protein. HBeAg(−): HBeAg-negative subjects; HBeAg(+): HBeAg-positive subjects; HIV+: HIV co-infected; HIV-: not co-infected with HIV.

    Journal: Virology Journal

    Article Title: Hepatitis B virus (HBV)-specific T-cell responses to recombinant HBV core protein in patients with normal liver function and co-infected with chronic HBV and human immunodeficiency virus 1 (HIV-1)

    doi: 10.1186/1743-422X-10-232

    Figure Lengend Snippet: Comparison of HBV-specific cell responses in the presence or absence of HBeAg. (A) C protein-specific T-cell responses elicited in ELISPOT assay; (B) Cytokine IFN-Y responses in cultured supernatants of PBMCs stimulated by C protein. HBeAg(−): HBeAg-negative subjects; HBeAg(+): HBeAg-positive subjects; HIV+: HIV co-infected; HIV-: not co-infected with HIV.

    Article Snippet: Human interferon (IFN)-γ ELISPOT assay Peripheral whole blood was obtained from all subjects and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with Ficoll Lymphoprep (Axis –Shield PoC AS, Oslo, Norway).

    Techniques: Enzyme-linked Immunospot, Cell Culture, Infection

    Chemical inhibition of ABCC6 increases the efficacy of dasatinib but not imatinib in patient MNCs. p-CRKL determined IC50 was determined via incubating patient MNCs with increasing concentrations of (A) dasatinib and (B) imatinib in the absence and presence of three ABCC6 inhibitors: pantoprazole, indomethacin, probenecid. CRKL western blotting was performed to determine the concentration of TKI required for 50% Bcr-Abl kinase inhibition (IC50). Representative western blots are shown in S3 and S4 Figs. The box plots depict the median, the upper 25th and the lower 75th percentiles while the whiskers encompass the 10th and 90th percentiles. Statistical analyses were performed using Student’s t -test with statistically p -values denoted by asterisks (** p

    Journal: PLoS ONE

    Article Title: ABCC6 plays a significant role in the transport of nilotinib and dasatinib, and contributes to TKI resistance in vitro, in both cell lines and primary patient mononuclear cells

    doi: 10.1371/journal.pone.0192180

    Figure Lengend Snippet: Chemical inhibition of ABCC6 increases the efficacy of dasatinib but not imatinib in patient MNCs. p-CRKL determined IC50 was determined via incubating patient MNCs with increasing concentrations of (A) dasatinib and (B) imatinib in the absence and presence of three ABCC6 inhibitors: pantoprazole, indomethacin, probenecid. CRKL western blotting was performed to determine the concentration of TKI required for 50% Bcr-Abl kinase inhibition (IC50). Representative western blots are shown in S3 and S4 Figs. The box plots depict the median, the upper 25th and the lower 75th percentiles while the whiskers encompass the 10th and 90th percentiles. Statistical analyses were performed using Student’s t -test with statistically p -values denoted by asterisks (** p

    Article Snippet: Patient cells Peripheral blood was obtained from de novo chronic phase CML patients before commencement of TKI therapy and mononuclear cells (MNCs) were isolated using Lymphoprep (Axis Shield, Oslo, Norway) density gradient centrifugation.

    Techniques: Inhibition, Western Blot, Concentration Assay