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Nycomed mononuclear cells mnc
MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis"

Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

Journal: Clinical and Experimental Immunology

doi: 10.1111/j.1365-2249.2004.02556.x

MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
Figure Legend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

Techniques Used: Centrifugation, Enzyme-linked Immunosorbent Assay

Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.
Figure Legend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

Techniques Used: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.
Figure Legend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

Techniques Used: Centrifugation, Staining, Flow Cytometry, Cytometry

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Gradient Centrifugation:

Article Title: Decreased serum lipocalin-2 levels in human immunodeficiency virus-infected patients: increase during highly active anti-retroviral therapy
Article Snippet: .. Heparinized bone marrow samples were obtained by aspiration from the posterior iliac crest and mononuclear cells (MNC) were prepared by density gradient centrifugation (Lymphoprep; Nycomed Pharma, Oslo, Norway). .. MNC from bone marrow aspirates were incubated in flat-bottomed 96-well trays (2 × 106 /ml; Costar, Cambridge, MA, USA), in medium alone [RPMI-1640 with 2 mM l -glutamine and 25 mM HEPES buffer (G ibco , Paisley, UK) supplemented with 10% of fetal calf serum (FCS); Sigma, St Louis, MO, USA)] or stimulated with phytohaemagglutinin (PHA); final dilution 1 : 100; Murex, Dartford, UK).

Article Title: Progressive polarization towards a T helper/cytotoxic type-1 cytokine pattern during age-dependent maturation of the immune response inversely correlates with CD30 cell expression and serum concentration
Article Snippet: Samples for flow cytometry studies were collected by venepuncture into preservative-free heparin. .. Mononuclear cells (MNC) were separated by density gradient centrifugation (Lymphoprep; Nycomed Pharma, Oslo, Norway) under sterile conditions. ..

Article Title: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis
Article Snippet: .. Mononuclear cells (MNC) were separated by Ficoll-Hypaque density gradient centrifugation (Lymphoprep, Nycomed, Norway). .. CD10+ B progenitor cells (ELP, pro-B and pre-B cells) were isolated from BM MNC using Dynabeads® M-450 Epoxy (Dynal, Oslo, Norway) directly coated with anti-CD10 mAb (clone RFAL-3, Sigma-Aldrich, UK) followed by detachment using CD4/CD8 DETACHaBEAD (Dynal, Norway) according to the producer 's protocol.

Article Title: Pimecrolimus does not affect the differentiation, maturation and function of human monocyte-derived dendritic cells, in contrast to corticosteroids
Article Snippet: The present study was carried out to compare the activity profile of pimecrolimus with that of CS regarding the inhibition of human monocyte-derived DC differentiation, terminal maturation and the modulation of their functions. .. Mononuclear cells (MNC) were isolated from leukapheresis samples of healthy human volunteers by density gradient centrifugation over Lymphoprep® (Nycomed Pharma, Oslo, Norway). .. Monocytes or primary CD4+ T cells or Pan T cells were purified from MNC aliquots by a negative immunomagnetic separation technique using the appropriate kits (Miltenyi Biotec, Bergisch Gladbach, Germany) allowing unmodified isolation of the target cells.

Article Title: Pimecrolimus inhibits up-regulation of OX40 and synthesis of inflammatory cytokines upon secondary T cell activation by allogeneic dendritic cells
Article Snippet: .. Mononuclear cells (MNC) were isolated from leukapheresis samples of healthy human volunteers by density gradient centrifugation over Lymphoprep® (Nycomed Pharma, Oslo, Norway). ..

Isolation:

Article Title: Spontaneous complement activation on human B cells results in localized membrane depolarization and the clustering of complement receptor type 2 and C3 fragments
Article Snippet: .. Mononuclear cells (MNC) were isolated by centrifugation, over Lymphoprep (Nycomed, Oslo, Norway), of blood drawn from healthy consenting donors into evacuated citrate–phosphate–dextrose (CPD)-containing tubes (Terumo, Leuvan, Belgium). .. Serum was harvested from the same donors, by collecting blood in anticoagulant-free tubes, which were held for 1 hr at 20° before centrifugation for 5 min at 400 g .

Article Title: Evidence for control of tumour necrosis factor-alpha (TNF-?) activity by TNF receptors in patients with proliferative diabetic retinopathy
Article Snippet: .. Mononuclear cells (MNC) from 38 patients and 11 controls were isolated from whole blood by gradient sedimentation on Lymphoprep (Nycomed Pharma AS, Oslo, Norway). .. MNC were cultured (1 × 106 cells/ml) for 24 h at 37°C in RPMI medium containing 5% fetal calf serum (FCS) and 1 ng/ml LPS.

Article Title: Pimecrolimus inhibits up-regulation of OX40 and synthesis of inflammatory cytokines upon secondary T cell activation by allogeneic dendritic cells
Article Snippet: .. Mononuclear cells (MNC) were isolated from leukapheresis samples of healthy human volunteers by density gradient centrifugation over Lymphoprep® (Nycomed Pharma, Oslo, Norway). ..

Centrifugation:

Article Title: Spontaneous complement activation on human B cells results in localized membrane depolarization and the clustering of complement receptor type 2 and C3 fragments
Article Snippet: .. Mononuclear cells (MNC) were isolated by centrifugation, over Lymphoprep (Nycomed, Oslo, Norway), of blood drawn from healthy consenting donors into evacuated citrate–phosphate–dextrose (CPD)-containing tubes (Terumo, Leuvan, Belgium). .. Serum was harvested from the same donors, by collecting blood in anticoagulant-free tubes, which were held for 1 hr at 20° before centrifugation for 5 min at 400 g .

Sedimentation:

Article Title: Evidence for control of tumour necrosis factor-alpha (TNF-?) activity by TNF receptors in patients with proliferative diabetic retinopathy
Article Snippet: .. Mononuclear cells (MNC) from 38 patients and 11 controls were isolated from whole blood by gradient sedimentation on Lymphoprep (Nycomed Pharma AS, Oslo, Norway). .. MNC were cultured (1 × 106 cells/ml) for 24 h at 37°C in RPMI medium containing 5% fetal calf serum (FCS) and 1 ng/ml LPS.

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    Nycomed mononuclear cells mnc
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc/product/Nycomed
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Nycomed peripheral blood mononuclear cells pbmc
    Dose–response curves for <t>ADCC.</t> The procedures for OxLDL incubated and Ab treatment were the same as described in the legend for Fig. . As effectors normal rat <t>PBMC</t> were added to the culture instead of complement. Note increased 51Cr release
    Peripheral Blood Mononuclear Cells Pbmc, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peripheral blood mononuclear cells pbmc/product/Nycomed
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peripheral blood mononuclear cells pbmc - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Nycomed treg peripheral blood mononuclear cells pbmcs
    TNFR2-agonist plus rapamycin expanded <t>Treg</t> inhibit inflammation in a humanised mouse model. (A) Schematic overview of the humanised skin inflammation mouse model used. In brief, SCID mice were transplanted with a human skin graft, 21 days after engraftment, PBS (as a control), or allogeneic human <t>PBMC</t> (huPBMC) only or huPBMC plus Treg of interest (at a ratio of 1:1) were injected intra peritoneally. 26 days later the animals were sacrificed to analyze the mouse spleen and human skin grafts. Rap Treg and R/T Treg refer to low purity MACS-isolated Treg expanded for 16-days in the presence of rapamycin-only or TNFR2-agonist plus rapamycin, respectively. (B) Representative photographs of spleens derived from mice infused with PBS, huPBMC only, or huPBMC plus Treg of interest, 21 days after the skin transplantation. Cumulative data showing the weight of spleens derived from N = 4 mice (right panel; n.d. = not determined). (C) Representative photographs showing histology (HE staining) of human skin grafts. Left panel: 10 x magnification. Right panel shows the epidermal thickness (μm) of human skin grafts following infusion of PBS, huPBMC, huPBMC plus Rap Treg or huPBMC plus R/T Treg. Mean ± SD, N = 6. (D) Immunohistochemistry of human CD3+ (brown) T cell infiltration in the human dermis. A representative photograph of N = 4 as presented in the cumulative data graph is shown (right panel; Mean ± SD). 20 x magnification. Wilcoxon paried t Test was used to compare the group of mice infused with huPBMC only with other groups of mice infused with huPBMC plus Treg of interest. Asterisks indicate significant differences.
    Treg Peripheral Blood Mononuclear Cells Pbmcs, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/treg peripheral blood mononuclear cells pbmcs/product/Nycomed
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    treg peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2021-03
    86/100 stars
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    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Enzyme-linked Immunosorbent Assay

    Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

    Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Staining, Flow Cytometry, Cytometry

    Dose–response curves for ADCC. The procedures for OxLDL incubated and Ab treatment were the same as described in the legend for Fig. . As effectors normal rat PBMC were added to the culture instead of complement. Note increased 51Cr release

    Journal: Cell Stress & Chaperones

    Article Title: Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis

    doi: 10.1007/s12192-013-0404-4

    Figure Lengend Snippet: Dose–response curves for ADCC. The procedures for OxLDL incubated and Ab treatment were the same as described in the legend for Fig. . As effectors normal rat PBMC were added to the culture instead of complement. Note increased 51Cr release

    Article Snippet: To determine antibody-dependent cellular cytotoxicity (ADCC), peripheral blood mononuclear cells (PBMC) were isolated from healthy rats by density centrifugation (Lymphoprep, density 1,083; Nycomed Pharmaceuticals Oslo, Norway) as described previously (Jurgens et al. ).

    Techniques: Incubation

    CD69 expression and proliferative capacity of CD4+ and CD8+ depending on the functional capacity. Whole blood from the BI groups of elders (group 0 + 1, n = 12 and group 2 + 3, n = 14) was stimulated for 18 h and expression of CD69 in CD4+ and CD8+ T cells was evaluated by flow cytometry. Proliferative capacity of CD4+ and CD8+ T cells subsets was also evaluated in the two groups (group 0 + 1, n = 19 and group 2 + 3, n = 16) by labeling the PBMC with CFSE. Cells were stained and 1 × 10 5 cells were acquired per experiment. a Representative dot plots showing the frequency of CD69 expression in CD4+ and CD8+ subset from elderly with different functional status. Cells were stimulated using anti-CD3 (10 ng/mL). Percentage of positive cells in each subpopulation in this representative experiment is expressed in the upper right corner and summarized results from all donors (median and IR) were also expressed in dot plots. b Proliferative capacity of CD4+ and CD8+ T cells subsets in response to anti-CD3. PBMC were labeled with CFSE (1.5 μM) and cultured in presence of anti-CD3 (10 ng/mL) for 5 days. Percentage of dividing CD4+ and CD8+ T cells is represented. Bars represent results from the grouped elders (mean ± SEM). c Expression of CD69 into de CD4+ and CD8+ T cell subset was analyzed in the same way as in Fig. 5a in response to a CMV supernatant (10 4 PFU/mL). d Proliferative capacity of CD4+ and CD8+ T-cell subsets in response to the CMV supernatant. Bars represent resulted from the grouped elders (mean ± SEM). The Student’s t test (when data were normally distributed) and Mann–Whitney non-parametric (when data were not normally distributed) methods were used to compare frequencies between groups. p values are depicted in the panels

    Journal: Age

    Article Title: Relationship between functional ability in older people, immune system status, and intensity of response to CMV

    doi: 10.1007/s11357-011-9240-6

    Figure Lengend Snippet: CD69 expression and proliferative capacity of CD4+ and CD8+ depending on the functional capacity. Whole blood from the BI groups of elders (group 0 + 1, n = 12 and group 2 + 3, n = 14) was stimulated for 18 h and expression of CD69 in CD4+ and CD8+ T cells was evaluated by flow cytometry. Proliferative capacity of CD4+ and CD8+ T cells subsets was also evaluated in the two groups (group 0 + 1, n = 19 and group 2 + 3, n = 16) by labeling the PBMC with CFSE. Cells were stained and 1 × 10 5 cells were acquired per experiment. a Representative dot plots showing the frequency of CD69 expression in CD4+ and CD8+ subset from elderly with different functional status. Cells were stimulated using anti-CD3 (10 ng/mL). Percentage of positive cells in each subpopulation in this representative experiment is expressed in the upper right corner and summarized results from all donors (median and IR) were also expressed in dot plots. b Proliferative capacity of CD4+ and CD8+ T cells subsets in response to anti-CD3. PBMC were labeled with CFSE (1.5 μM) and cultured in presence of anti-CD3 (10 ng/mL) for 5 days. Percentage of dividing CD4+ and CD8+ T cells is represented. Bars represent results from the grouped elders (mean ± SEM). c Expression of CD69 into de CD4+ and CD8+ T cell subset was analyzed in the same way as in Fig. 5a in response to a CMV supernatant (10 4 PFU/mL). d Proliferative capacity of CD4+ and CD8+ T-cell subsets in response to the CMV supernatant. Bars represent resulted from the grouped elders (mean ± SEM). The Student’s t test (when data were normally distributed) and Mann–Whitney non-parametric (when data were not normally distributed) methods were used to compare frequencies between groups. p values are depicted in the panels

    Article Snippet: To analyze the proliferation status of CD4+ and CD8+ T cells, peripheral blood mononuclear cells (PBMC) were isolated by centrifugation on Ficoll-Hypaque gradients (Lymphoprep; Nycomed, Oslo, Norway).

    Techniques: Expressing, Functional Assay, Flow Cytometry, Cytometry, Labeling, Staining, Cell Culture, MANN-WHITNEY

    TNFR2-agonist plus rapamycin expanded Treg inhibit inflammation in a humanised mouse model. (A) Schematic overview of the humanised skin inflammation mouse model used. In brief, SCID mice were transplanted with a human skin graft, 21 days after engraftment, PBS (as a control), or allogeneic human PBMC (huPBMC) only or huPBMC plus Treg of interest (at a ratio of 1:1) were injected intra peritoneally. 26 days later the animals were sacrificed to analyze the mouse spleen and human skin grafts. Rap Treg and R/T Treg refer to low purity MACS-isolated Treg expanded for 16-days in the presence of rapamycin-only or TNFR2-agonist plus rapamycin, respectively. (B) Representative photographs of spleens derived from mice infused with PBS, huPBMC only, or huPBMC plus Treg of interest, 21 days after the skin transplantation. Cumulative data showing the weight of spleens derived from N = 4 mice (right panel; n.d. = not determined). (C) Representative photographs showing histology (HE staining) of human skin grafts. Left panel: 10 x magnification. Right panel shows the epidermal thickness (μm) of human skin grafts following infusion of PBS, huPBMC, huPBMC plus Rap Treg or huPBMC plus R/T Treg. Mean ± SD, N = 6. (D) Immunohistochemistry of human CD3+ (brown) T cell infiltration in the human dermis. A representative photograph of N = 4 as presented in the cumulative data graph is shown (right panel; Mean ± SD). 20 x magnification. Wilcoxon paried t Test was used to compare the group of mice infused with huPBMC only with other groups of mice infused with huPBMC plus Treg of interest. Asterisks indicate significant differences.

    Journal: PLoS ONE

    Article Title: A TNFR2-Agonist Facilitates High Purity Expansion of Human Low Purity Treg Cells

    doi: 10.1371/journal.pone.0156311

    Figure Lengend Snippet: TNFR2-agonist plus rapamycin expanded Treg inhibit inflammation in a humanised mouse model. (A) Schematic overview of the humanised skin inflammation mouse model used. In brief, SCID mice were transplanted with a human skin graft, 21 days after engraftment, PBS (as a control), or allogeneic human PBMC (huPBMC) only or huPBMC plus Treg of interest (at a ratio of 1:1) were injected intra peritoneally. 26 days later the animals were sacrificed to analyze the mouse spleen and human skin grafts. Rap Treg and R/T Treg refer to low purity MACS-isolated Treg expanded for 16-days in the presence of rapamycin-only or TNFR2-agonist plus rapamycin, respectively. (B) Representative photographs of spleens derived from mice infused with PBS, huPBMC only, or huPBMC plus Treg of interest, 21 days after the skin transplantation. Cumulative data showing the weight of spleens derived from N = 4 mice (right panel; n.d. = not determined). (C) Representative photographs showing histology (HE staining) of human skin grafts. Left panel: 10 x magnification. Right panel shows the epidermal thickness (μm) of human skin grafts following infusion of PBS, huPBMC, huPBMC plus Rap Treg or huPBMC plus R/T Treg. Mean ± SD, N = 6. (D) Immunohistochemistry of human CD3+ (brown) T cell infiltration in the human dermis. A representative photograph of N = 4 as presented in the cumulative data graph is shown (right panel; Mean ± SD). 20 x magnification. Wilcoxon paried t Test was used to compare the group of mice infused with huPBMC only with other groups of mice infused with huPBMC plus Treg of interest. Asterisks indicate significant differences.

    Article Snippet: Isolation of Treg Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Lymphoprep, Nycomed Pharma AS, Oslo, Norway) of buffy coats obtained from healthy blood donors (Sanquin Blood Bank, Region South-East, Netherlands) upon written informed consent, according to the Dutch law.

    Techniques: Mouse Assay, Injection, Magnetic Cell Separation, Isolation, Derivative Assay, Transplantation Assay, Staining, Immunohistochemistry