mononuclear cells mnc (Nycomed)
Structured Review

Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mononuclear cells mnc/product/Nycomed
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis"
Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis
Journal: Clinical and Experimental Immunology
doi: 10.1111/j.1365-2249.2004.02556.x

Figure Legend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
Techniques Used: Centrifugation, Enzyme-linked Immunosorbent Assay

Figure Legend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.
Techniques Used: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

Figure Legend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.
Techniques Used: Centrifugation, Staining, Flow Cytometry, Cytometry
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