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Nycomed mononuclear cells mnc
MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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91/100 stars

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1) Product Images from "CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis"

Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

Journal: Clinical and Experimental Immunology

doi: 10.1111/j.1365-2249.2004.02556.x

MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
Figure Legend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

Techniques Used: Centrifugation, Enzyme-linked Immunosorbent Assay

Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.
Figure Legend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

Techniques Used: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.
Figure Legend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

Techniques Used: Centrifugation, Staining, Flow Cytometry, Cytometry

Related Articles

Isolation:

Article Title: Defining Early Human NK Cell Developmental Stages in Primary and Secondary Lymphoid Tissues
Article Snippet: .. Mononuclear cells (MNC) were isolated by density gradient centrifugation (Lymphoprep; Nycomed Pharma, Roskilde, Denmark) and cryopreserved in liquid nitrogen until further use. ..

Article Title: Spontaneous complement activation on human B cells results in localized membrane depolarization and the clustering of complement receptor type 2 and C3 fragments
Article Snippet: .. Mononuclear cells (MNC) were isolated by centrifugation, over Lymphoprep (Nycomed, Oslo, Norway), of blood drawn from healthy consenting donors into evacuated citrate–phosphate–dextrose (CPD)-containing tubes (Terumo, Leuvan, Belgium). .. Serum was harvested from the same donors, by collecting blood in anticoagulant-free tubes, which were held for 1 hr at 20° before centrifugation for 5 min at 400 g .

Article Title: Evidence for control of tumour necrosis factor-alpha (TNF-?) activity by TNF receptors in patients with proliferative diabetic retinopathy
Article Snippet: .. Mononuclear cells (MNC) from 38 patients and 11 controls were isolated from whole blood by gradient sedimentation on Lymphoprep (Nycomed Pharma AS, Oslo, Norway). .. MNC were cultured (1 × 106 cells/ml) for 24 h at 37°C in RPMI medium containing 5% fetal calf serum (FCS) and 1 ng/ml LPS.

Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis
Article Snippet: .. Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway). .. T cells were separated using positive selection of antirat CD3 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany).

Article Title: Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells
Article Snippet: .. Mononuclear cells (MNC) and monocytes were isolated from PWB using differential centrifugation with Lymphoprep (Nycomed, Oslo, Norway) and negative monocyte isolation kit (Dynal, Wirral, UK), respectively, as described previously [ ]. .. Monocytes and MNC number were determined using a haemocytometer (Neubauer; Weber Scientific Ltd, Teddington, UK).

Centrifugation:

Article Title: Spontaneous complement activation on human B cells results in localized membrane depolarization and the clustering of complement receptor type 2 and C3 fragments
Article Snippet: .. Mononuclear cells (MNC) were isolated by centrifugation, over Lymphoprep (Nycomed, Oslo, Norway), of blood drawn from healthy consenting donors into evacuated citrate–phosphate–dextrose (CPD)-containing tubes (Terumo, Leuvan, Belgium). .. Serum was harvested from the same donors, by collecting blood in anticoagulant-free tubes, which were held for 1 hr at 20° before centrifugation for 5 min at 400 g .

Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis
Article Snippet: .. Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway). .. T cells were separated using positive selection of antirat CD3 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany).

Article Title: Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells
Article Snippet: .. Mononuclear cells (MNC) and monocytes were isolated from PWB using differential centrifugation with Lymphoprep (Nycomed, Oslo, Norway) and negative monocyte isolation kit (Dynal, Wirral, UK), respectively, as described previously [ ]. .. Monocytes and MNC number were determined using a haemocytometer (Neubauer; Weber Scientific Ltd, Teddington, UK).

Gradient Centrifugation:

Article Title: Defining Early Human NK Cell Developmental Stages in Primary and Secondary Lymphoid Tissues
Article Snippet: .. Mononuclear cells (MNC) were isolated by density gradient centrifugation (Lymphoprep; Nycomed Pharma, Roskilde, Denmark) and cryopreserved in liquid nitrogen until further use. ..

Article Title: The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System
Article Snippet: .. Cell Isolation and DC Generation Buffy coats were obtained from healthy donors and were used to isolate mononuclear cells (MNC), by gradient centrifugation (Lymphoprep; 1.077 g/mL; Nycomed Pharma). .. After separation, CD14+ monocytes and CD3+ T cells were purified from total MNC by magnetic separation columns (Miltenyi Biotec), according to manufacturer's instructions.

Article Title: Wnt expression and canonical Wnt signaling in human bone marrow B lymphopoiesis
Article Snippet: .. Mononuclear cells (MNC) were separated by Ficoll-Hypaque density gradient centrifugation (Lymphoprep, Nycomed, Norway). .. CD10+ B progenitor cells (ELP, pro-B and pre-B cells) were isolated from BM MNC using Dynabeads® M-450 Epoxy (Dynal, Oslo, Norway) directly coated with anti-CD10 mAb (clone RFAL-3, Sigma-Aldrich, UK) followed by detachment using CD4/CD8 DETACHaBEAD (Dynal, Norway) according to the producer 's protocol.

Article Title: Chemokine receptor CCR2 is expressed by human multiple myeloma cells and mediates migration to bone marrow stromal cell-produced monocyte chemotactic proteins MCP-1, -2 and -3
Article Snippet: .. Mononuclear cells (MNC) were separated by Ficoll density gradient centrifugation (Nycomed, Lucron, Gent, Belgium). .. MACS separation of primary MM cells Primary MM cells were immunomagnetically separated using the magnetic cell sorting system (MACS) (Miltenyi Biotech Sanvertech, Bouchout, Belgium).

Cell Isolation:

Article Title: The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System
Article Snippet: .. Cell Isolation and DC Generation Buffy coats were obtained from healthy donors and were used to isolate mononuclear cells (MNC), by gradient centrifugation (Lymphoprep; 1.077 g/mL; Nycomed Pharma). .. After separation, CD14+ monocytes and CD3+ T cells were purified from total MNC by magnetic separation columns (Miltenyi Biotec), according to manufacturer's instructions.

Sedimentation:

Article Title: Evidence for control of tumour necrosis factor-alpha (TNF-?) activity by TNF receptors in patients with proliferative diabetic retinopathy
Article Snippet: .. Mononuclear cells (MNC) from 38 patients and 11 controls were isolated from whole blood by gradient sedimentation on Lymphoprep (Nycomed Pharma AS, Oslo, Norway). .. MNC were cultured (1 × 106 cells/ml) for 24 h at 37°C in RPMI medium containing 5% fetal calf serum (FCS) and 1 ng/ml LPS.

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    Nycomed pbmc collection
    Cancer testis antigen (CTA)-specific T cells in the peripheral blood of patients. Detection of CTA- or viral-specific T cells in <t>PBMCs</t> by MHC multimers, expressed in percentage of CD8 T cells. ( a ) The sum of CTA-specific T cells as measured in peripheral blood at different time points during treatment. Eight patients were tested, results from the six patients with detectable responses are shown. First cycle represents a sample obtained before treatment. The following responses were found for each patient: AZA 1 (SART-3 WLE , SART-3 QIR , Sp17 ILD ), AZA 2 (MAGE-A2 LVH , MAGE-A2 KMV , TAG-1 SLG ), AZA 4 (MAGE-A2 LVH , MAGE-A2 LVQ , NY-ESO-1 QLS ), AZA 5 (MAGE-A1 EAD ), AZA 12 (MAGE-A2 LVH , MAGE-A2 KMV , CDCA1 KLA , TAG-1 SLG , NY-ESO-1 SLL , MAGE-A1 EAD ) and AZA 16 (MAGE-A2 LVH , MAGE-A2 KMV , GnTV VLP , TAG-1 SLG ). ( b ) The frequency of individual CTA-specific T cells detected after an in vitro peptide pre-stimulation was performed at different time points during treatment. ( c ) The sum of virus-specific T cells detected over the course of treatment. The following responses were detected: AZA 1 (EBV RLR , EBV RLR , FLU ILR ), AZA 2 (EBV GLC , FLU ILR ), AZA 4 (EBV GLC , EBV YVL , FLU GIL ), AZA 5 (FLU BP-VSD ), AZA 12 (CMV VTE , CMV YSE , CMV NLV , FLU GIL ), AZA 14 (CMV YSE , CMV VTE , FLU BP-VSD ), AZA 16 (CMV NLV , EBV GLC ) and AZA 17 (CMV YSE , CMV VTE , FLU BP-VSD ). MHC-multimer-specific T cells are given in percentage of CD8 cells. Significance is indicated by * P
    Pbmc Collection, supplied by Nycomed, used in various techniques. Bioz Stars score: 88/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc collection/product/Nycomed
    Average 88 stars, based on 203 article reviews
    Price from $9.99 to $1999.99
    pbmc collection - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    91
    Nycomed mononuclear cells mnc
    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and <t>MNC</t> were prepared by centrifugation over <t>Lymphoprep</t> density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P
    Mononuclear Cells Mnc, supplied by Nycomed, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mnc/product/Nycomed
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Cancer testis antigen (CTA)-specific T cells in the peripheral blood of patients. Detection of CTA- or viral-specific T cells in PBMCs by MHC multimers, expressed in percentage of CD8 T cells. ( a ) The sum of CTA-specific T cells as measured in peripheral blood at different time points during treatment. Eight patients were tested, results from the six patients with detectable responses are shown. First cycle represents a sample obtained before treatment. The following responses were found for each patient: AZA 1 (SART-3 WLE , SART-3 QIR , Sp17 ILD ), AZA 2 (MAGE-A2 LVH , MAGE-A2 KMV , TAG-1 SLG ), AZA 4 (MAGE-A2 LVH , MAGE-A2 LVQ , NY-ESO-1 QLS ), AZA 5 (MAGE-A1 EAD ), AZA 12 (MAGE-A2 LVH , MAGE-A2 KMV , CDCA1 KLA , TAG-1 SLG , NY-ESO-1 SLL , MAGE-A1 EAD ) and AZA 16 (MAGE-A2 LVH , MAGE-A2 KMV , GnTV VLP , TAG-1 SLG ). ( b ) The frequency of individual CTA-specific T cells detected after an in vitro peptide pre-stimulation was performed at different time points during treatment. ( c ) The sum of virus-specific T cells detected over the course of treatment. The following responses were detected: AZA 1 (EBV RLR , EBV RLR , FLU ILR ), AZA 2 (EBV GLC , FLU ILR ), AZA 4 (EBV GLC , EBV YVL , FLU GIL ), AZA 5 (FLU BP-VSD ), AZA 12 (CMV VTE , CMV YSE , CMV NLV , FLU GIL ), AZA 14 (CMV YSE , CMV VTE , FLU BP-VSD ), AZA 16 (CMV NLV , EBV GLC ) and AZA 17 (CMV YSE , CMV VTE , FLU BP-VSD ). MHC-multimer-specific T cells are given in percentage of CD8 cells. Significance is indicated by * P

    Journal: Blood Cancer Journal

    Article Title: 5-Azacytidine treatment sensitizes tumor cells to T-cell mediated cytotoxicity and modulates NK cells in patients with myeloid malignancies

    doi: 10.1038/bcj.2014.14

    Figure Lengend Snippet: Cancer testis antigen (CTA)-specific T cells in the peripheral blood of patients. Detection of CTA- or viral-specific T cells in PBMCs by MHC multimers, expressed in percentage of CD8 T cells. ( a ) The sum of CTA-specific T cells as measured in peripheral blood at different time points during treatment. Eight patients were tested, results from the six patients with detectable responses are shown. First cycle represents a sample obtained before treatment. The following responses were found for each patient: AZA 1 (SART-3 WLE , SART-3 QIR , Sp17 ILD ), AZA 2 (MAGE-A2 LVH , MAGE-A2 KMV , TAG-1 SLG ), AZA 4 (MAGE-A2 LVH , MAGE-A2 LVQ , NY-ESO-1 QLS ), AZA 5 (MAGE-A1 EAD ), AZA 12 (MAGE-A2 LVH , MAGE-A2 KMV , CDCA1 KLA , TAG-1 SLG , NY-ESO-1 SLL , MAGE-A1 EAD ) and AZA 16 (MAGE-A2 LVH , MAGE-A2 KMV , GnTV VLP , TAG-1 SLG ). ( b ) The frequency of individual CTA-specific T cells detected after an in vitro peptide pre-stimulation was performed at different time points during treatment. ( c ) The sum of virus-specific T cells detected over the course of treatment. The following responses were detected: AZA 1 (EBV RLR , EBV RLR , FLU ILR ), AZA 2 (EBV GLC , FLU ILR ), AZA 4 (EBV GLC , EBV YVL , FLU GIL ), AZA 5 (FLU BP-VSD ), AZA 12 (CMV VTE , CMV YSE , CMV NLV , FLU GIL ), AZA 14 (CMV YSE , CMV VTE , FLU BP-VSD ), AZA 16 (CMV NLV , EBV GLC ) and AZA 17 (CMV YSE , CMV VTE , FLU BP-VSD ). MHC-multimer-specific T cells are given in percentage of CD8 cells. Significance is indicated by * P

    Article Snippet: PBMC collection and processing Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by gradient centrifugation (Lymphoprep, 1.077 g/ml, Nycomed Pharma AS, Oslo, Norway) at room temperature within a few hours after the blood was obtained from the patient.

    Techniques: In Vitro, Gas Chromatography

    CD4 % is lower in PBMC than in LNMC in HIV infection

    Journal: Clinical and Experimental Immunology

    Article Title: Proviral load and immune function in blood and lymph node during HIV-1 and HIV-2 infection

    doi: 10.1046/j.1365-2249.1999.00914.x

    Figure Lengend Snippet: CD4 % is lower in PBMC than in LNMC in HIV infection

    Article Snippet: PBMC and LNMC were purified by density centrifugation on Lymphoprep (Nycomed, Oslo, Norway).

    Techniques: Infection

    IFN-α stimulated hepatocytes are still able to act as APCs to CTL. HepG2 cells were stimulated for 16 hours with 1000 U/ml IFN-α, or left untreated. The cells were then pulsed with the specific cognate peptide and washed, prior to co-incubation with the CTL line 2. As controls non-peptide-pulsed cells were used, or all PBMC were peptide pulsed or stimulated with PMA/ionomycin. A) The raw FACS data showing the CD8+ expression on the y axis's and IFN-γ on the x axis's. B) The percentage secretion data from such experiment. HepG2p represents peptide pulsed cells with or without IFN-α stimulation as indicated. This data is representative of 4 such experiments.

    Journal: PLoS ONE

    Article Title: Protection of Hepatocytes from Cytotoxic T Cell Mediated Killing by Interferon-Alpha

    doi: 10.1371/journal.pone.0000791

    Figure Lengend Snippet: IFN-α stimulated hepatocytes are still able to act as APCs to CTL. HepG2 cells were stimulated for 16 hours with 1000 U/ml IFN-α, or left untreated. The cells were then pulsed with the specific cognate peptide and washed, prior to co-incubation with the CTL line 2. As controls non-peptide-pulsed cells were used, or all PBMC were peptide pulsed or stimulated with PMA/ionomycin. A) The raw FACS data showing the CD8+ expression on the y axis's and IFN-γ on the x axis's. B) The percentage secretion data from such experiment. HepG2p represents peptide pulsed cells with or without IFN-α stimulation as indicated. This data is representative of 4 such experiments.

    Article Snippet: Isolation of Peripheral Blood Mononuclear Cells (PBMC): PBMCs were isolated from whole blood collected and mixed with either heparin or EDTA, and separated by density gradient centrifugation over lymphoprep (Nycomed, UK).

    Techniques: Activated Clotting Time Assay, CTL Assay, Incubation, FACS, Expressing

    MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: MBP87–99- and MBP68–86-induced cytokine levels. Blood was obtained on day 12 p.i., and MNC were prepared by centrifugation over Lymphoprep density gradient. IFN- γ , IL-10 and TNF- α were measured by ELISA kits. Data are presented as the mean ± s.d. of duplicate samples from three to four rats/Exp. 2 (* P

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Enzyme-linked Immunosorbent Assay

    Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Frequency of CD4 + T cell expressing IL-10. Blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. Immediately following separation, CD4 + T cells expressing IL-10 were stained with FITC-CD4 and PE-IL-10 antibodies, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Expressing, Centrifugation, Staining, Flow Cytometry, Cytometry

    Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8α+ dendritic cells and immune protection from experimental allergic encephalomyelitis

    doi: 10.1111/j.1365-2249.2004.02556.x

    Figure Lengend Snippet: Phenotypic analysis of MNC. To examine phenotypic diversity of MNC, blood was obtained on day 12 p.i. (3–4 rats/Exp. 2), and MNC were prepared by centrifugation over Lymphoprep density gradient. MNC were stained for CD4, CD8, CD28, CD40, CD80, CD86 and MHC class II, and analysed by flow cytometry. Representative patterns are shown.

    Article Snippet: Peripheral blood was obtained on day 12 p.i., and mononuclear cells (MNC) were isolated by centrifugation over Lymphoprep density gradient (Nycomed, Oslo, Norway).

    Techniques: Centrifugation, Staining, Flow Cytometry, Cytometry