mononuclear cells mnc  (Millipore)


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    Structured Review

    Millipore mononuclear cells mnc
    Purity of <t>CD34+</t> cells before expansion. After isolation of cord blood cells, the surface marker CD34 was assessed immediately. A) Isotypecontrol, B) Percentage of CD34+ cells from <t>MNC</t> isolated cord blood
    Mononuclear Cells Mnc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mnc - by Bioz Stars, 2020-09
    99/100 stars

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    1) Product Images from "Expansion of human cord blood hematopoietic stem/progenitor cells in three-dimensional Nanoscaffold coated with Fibronectin"

    Article Title: Expansion of human cord blood hematopoietic stem/progenitor cells in three-dimensional Nanoscaffold coated with Fibronectin

    Journal: International Journal of Hematology-Oncology and Stem Cell Research

    doi:

    Purity of CD34+ cells before expansion. After isolation of cord blood cells, the surface marker CD34 was assessed immediately. A) Isotypecontrol, B) Percentage of CD34+ cells from MNC isolated cord blood
    Figure Legend Snippet: Purity of CD34+ cells before expansion. After isolation of cord blood cells, the surface marker CD34 was assessed immediately. A) Isotypecontrol, B) Percentage of CD34+ cells from MNC isolated cord blood

    Techniques Used: Isolation, Marker

    Related Articles

    Flow Cytometry:

    Article Title: Immune characterization of metastatic colorectal cancer patients post reovirus administration
    Article Snippet: .. Peripheral blood mononuclear cells were stained with fluorophore labelled antibodies against CD4, CD8, CD56, CD70, and CD123, fixed and evaluated by flow cytometry. .. The expression of granzyme B was determined on core biopsy of one patient.

    Isolation:

    Article Title: Knockdown of LSD1 ameliorates the severity of rheumatoid arthritis and decreases the function of CD4 T cells in mouse models
    Article Snippet: .. Next, mononuclear cells in the SF samples were isolated using Ficoll (Sigma-Aldrich) density gradient centrifugation. .. CD4+ T cells in mononuclear cells were isolated using a CD4+ T Cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor, et al. Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor
    Article Snippet: .. Peripheral blood mononuclear cells were isolated from the whole blood of healthy donors by Ficoll density gradient centrifugation. .. NK cells were obtained by stimulating the PBMCs in the NK cell‐specific medium (Dakewe) with 5% human serum (Sigma) for 14 days.

    Centrifugation:

    Article Title: Mechanisms of antileukemic activity of the novel BH3 mimetic GX15-070 (obatoclax)
    Article Snippet: .. Mononuclear cells were separated by Ficoll-Hypaque (Sigma Chemical Co.) density-gradient centrifugation. .. Cells were either used for colony assays, as described below, or cultured in AIM-V medium (Gibco Laboratories, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Gemini Bio-Products, Woodland, CA), 1mM L-glutamine (Gibco Laboratories), and 50ug/ml penicillin/streptomycin (Gibco Laboratories).

    other:

    Article Title: SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst
    Article Snippet: Cells were layered over Ficoll-Paque-Plus (Pharmacia), and centrifuged at 400 x g for 25 min at RT without applying the break to enrich for mononuclear cells.

    Article Title: Human Immunodeficiency Virus-Negative Men Who Have Sex With Men Have an Altered T-Cell Phenotype and Bioenergy Metabolism
    Article Snippet: Peripheral blood mononuclear cells were plated in 4 replicate wells in poly-d -lysine-coated Seahorse XFe96 wells plate at a density of 200 000 cells/well.

    Article Title: Immunophenotyping of Peripheral Blood, Lymph Node, and Bone Marrow T Lymphocytes During Canine Leishmaniosis and the Impact of Antileishmanial Chemotherapy
    Article Snippet: Peripheral blood mononuclear cells were then harvested at the interface of PBS and Histopaque® and washed twice in cold PBS (300 × g, 10 min, 4°C).

    Staining:

    Article Title: Immune characterization of metastatic colorectal cancer patients post reovirus administration
    Article Snippet: .. Peripheral blood mononuclear cells were stained with fluorophore labelled antibodies against CD4, CD8, CD56, CD70, and CD123, fixed and evaluated by flow cytometry. .. The expression of granzyme B was determined on core biopsy of one patient.

    Gradient Centrifugation:

    Article Title: Knockdown of LSD1 ameliorates the severity of rheumatoid arthritis and decreases the function of CD4 T cells in mouse models
    Article Snippet: .. Next, mononuclear cells in the SF samples were isolated using Ficoll (Sigma-Aldrich) density gradient centrifugation. .. CD4+ T cells in mononuclear cells were isolated using a CD4+ T Cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor, et al. Targeting epidermal growth factor‐overexpressing triple‐negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor
    Article Snippet: .. Peripheral blood mononuclear cells were isolated from the whole blood of healthy donors by Ficoll density gradient centrifugation. .. NK cells were obtained by stimulating the PBMCs in the NK cell‐specific medium (Dakewe) with 5% human serum (Sigma) for 14 days.

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    Millipore culture pbmcs
    Features of ssRNA40-induced LILRA3 expression. a Kinetics of LILRA3 expression was compared to other ssRNA40 inducible cytokines. <t>PBMCs</t> from two donors were stimulated with ssRNA40 or ssRNA41 and the RNA was harvested at various time points. qPCR for LILRA3 , TNF , IFNb , IFNg , and IL1b expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (pooled cDNA from 10. b Effect of cytokine inhibition on LILRA3 expression. PBMCs from two donors were stimulated overnight with ssRNA40 together with neutralizing agents anakinra, certolizumab, tocilizumab and IFN-γ neutralizing antibodies and analysed for LILRA3 and IL6 expression by qPCR. Repeated measures ANOVA was used to calculate difference of ssRNA40+ neutralization to ssRNA40 alone and results displayed as mean ± SD
    Culture Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    culture pbmcs - by Bioz Stars, 2020-09
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    85
    Millipore pbmc specimens against sd1 lps
    Fecal s-IgA antibody titers to <t>SD1-LPS</t> in group1 (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol) monkeys, expressed as MN with MN ± SE, after challenge on day 00 and re-challenge on day 31 with SD11617 strain.
    Pbmc Specimens Against Sd1 Lps, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore human pbmcs
    Imatinib inhibits TNF-α production in human myeloid cells. Human <t>PBMCs,</t> monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml <t>LPS.</t> ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.
    Human Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pbmcs/product/Millipore
    Average 93 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
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    94
    Millipore host pbmcs
    Effects of the various concentrations of <t>rHcMTF-12</t> on <t>PBMC</t> migration in vitro . Cells were treated with control buffer, pET32a protein and multiple concentrations of rHcMTF-12. The migration percentage was determined randomly. The difference between the mean values was calculated using ANOVA. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. Data are representative of three independent experiments. * P
    Host Pbmcs, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/host pbmcs/product/Millipore
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    Features of ssRNA40-induced LILRA3 expression. a Kinetics of LILRA3 expression was compared to other ssRNA40 inducible cytokines. PBMCs from two donors were stimulated with ssRNA40 or ssRNA41 and the RNA was harvested at various time points. qPCR for LILRA3 , TNF , IFNb , IFNg , and IL1b expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (pooled cDNA from 10. b Effect of cytokine inhibition on LILRA3 expression. PBMCs from two donors were stimulated overnight with ssRNA40 together with neutralizing agents anakinra, certolizumab, tocilizumab and IFN-γ neutralizing antibodies and analysed for LILRA3 and IL6 expression by qPCR. Repeated measures ANOVA was used to calculate difference of ssRNA40+ neutralization to ssRNA40 alone and results displayed as mean ± SD

    Journal: Retrovirology

    Article Title: TLR8 regulation of LILRA3 in monocytes is abrogated in human immunodeficiency virus infection and correlates to CD4 counts and virus loads

    doi: 10.1186/s12977-016-0248-y

    Figure Lengend Snippet: Features of ssRNA40-induced LILRA3 expression. a Kinetics of LILRA3 expression was compared to other ssRNA40 inducible cytokines. PBMCs from two donors were stimulated with ssRNA40 or ssRNA41 and the RNA was harvested at various time points. qPCR for LILRA3 , TNF , IFNb , IFNg , and IL1b expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (pooled cDNA from 10. b Effect of cytokine inhibition on LILRA3 expression. PBMCs from two donors were stimulated overnight with ssRNA40 together with neutralizing agents anakinra, certolizumab, tocilizumab and IFN-γ neutralizing antibodies and analysed for LILRA3 and IL6 expression by qPCR. Repeated measures ANOVA was used to calculate difference of ssRNA40+ neutralization to ssRNA40 alone and results displayed as mean ± SD

    Article Snippet: PBMC and monocyte isolation and culture PBMCs were isolated using density gradient centrifugation by layering blood onto Biocoll (Millipore) and centrifugation at 1200×g for 20 min without brake.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Inhibition, Neutralization

    LILRA3 induction by TLRs. a Induction of LILRA3 and IL6 expression by a panel of TLR agonists. LILRA3 and IL6 expression was measured, using qPCR, as fold change to the unstimulated control from PBMCs stimulated for 24 h with Pam3CSK4 (P3C), heat killed Listeria monocytogenes (HKLM), polyinosinic-polycytidylic acid (Poly I:C) in high molecular weight (HMW) and low molecular weight (LMW) forms, lipopolysaccharide (LPS), flagellin, synthetic diacylated lipoprotein FSL-1, Imiquimod (R837), ssRNA40/LyoVec and CpG oligonucleotide ODN 2006. b Induction of LILRA3 by ssRNA40 on the transcriptional level. LILRA3 expression was measured as fold change to the unstimulated control from PBMCs stimulated for 24 h with uridine-rich HIV-derived ssRNA40, using ssRNA41 as control (n = 8). Wilcoxon matched-pairs signed rank test was used to compare the median between ssRNA41 and ssRNA40 stimulated LILRA3 expression. Scatter dot plot overlayed on bar graph displayed as median with interquartile range. c Induction of LILRA3 in PBMCS by ssRNA40 on the protein level. 30 × 10 6 /mL PBMCs from LILRA3 + / + and LILRA3 − / − donors were stimulated with 5 μg/mL ssRNA40. 40 µL from each supernatant was loaded and Ponceau S staining was used as loading control. The membrane was blotted for LILRA3 and IL6, using recombinant LILRA3 as protein control. d Expression of ssRNA40-induced LILRA3 by monocytes. PBMCs stimulated overnight with ssRNA40 and LPS were purified for CD14 + monocytes using magnetic-activated cell sorting. The resulting CD14 + positive and depleted fractions were analysed via qPCR for LILRA3 expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (*p

    Journal: Retrovirology

    Article Title: TLR8 regulation of LILRA3 in monocytes is abrogated in human immunodeficiency virus infection and correlates to CD4 counts and virus loads

    doi: 10.1186/s12977-016-0248-y

    Figure Lengend Snippet: LILRA3 induction by TLRs. a Induction of LILRA3 and IL6 expression by a panel of TLR agonists. LILRA3 and IL6 expression was measured, using qPCR, as fold change to the unstimulated control from PBMCs stimulated for 24 h with Pam3CSK4 (P3C), heat killed Listeria monocytogenes (HKLM), polyinosinic-polycytidylic acid (Poly I:C) in high molecular weight (HMW) and low molecular weight (LMW) forms, lipopolysaccharide (LPS), flagellin, synthetic diacylated lipoprotein FSL-1, Imiquimod (R837), ssRNA40/LyoVec and CpG oligonucleotide ODN 2006. b Induction of LILRA3 by ssRNA40 on the transcriptional level. LILRA3 expression was measured as fold change to the unstimulated control from PBMCs stimulated for 24 h with uridine-rich HIV-derived ssRNA40, using ssRNA41 as control (n = 8). Wilcoxon matched-pairs signed rank test was used to compare the median between ssRNA41 and ssRNA40 stimulated LILRA3 expression. Scatter dot plot overlayed on bar graph displayed as median with interquartile range. c Induction of LILRA3 in PBMCS by ssRNA40 on the protein level. 30 × 10 6 /mL PBMCs from LILRA3 + / + and LILRA3 − / − donors were stimulated with 5 μg/mL ssRNA40. 40 µL from each supernatant was loaded and Ponceau S staining was used as loading control. The membrane was blotted for LILRA3 and IL6, using recombinant LILRA3 as protein control. d Expression of ssRNA40-induced LILRA3 by monocytes. PBMCs stimulated overnight with ssRNA40 and LPS were purified for CD14 + monocytes using magnetic-activated cell sorting. The resulting CD14 + positive and depleted fractions were analysed via qPCR for LILRA3 expression, normalized to RPLP0 and relatively quantified to calibrator cDNA (*p

    Article Snippet: PBMC and monocyte isolation and culture PBMCs were isolated using density gradient centrifugation by layering blood onto Biocoll (Millipore) and centrifugation at 1200×g for 20 min without brake.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Molecular Weight, Derivative Assay, Staining, Recombinant, Purification, FACS

    LILRA3 stimulation of cytokines and antigen presenting cells. a qPCR of LILRA3 induced gene expression of LILRA3 , IL - 6 , IL - 1A , IL - 1B and IL - 10 . Due to the different optimal concentrations of LILRA3 for various donors, the gene expression of all four donors were shown, with the corresponding mean ± SD. b Effect of LILRA3 on the antigen presentation mechanism of monocytes and B-cells. PBMCs were stimulated with varying concentrations of LILRA3 for 48 h and analysed by flow cytometry for expression of CD80, CD86, HLA-DR and HLA-ABC on monocytes (CD14 + CD33 + ) and B-cells (CD3 + CD19 + ). On monocytes, upregulation of HLA-ABC and CD80 was observed, whereas CD86 was downregulated. On B-cells, there was a slight but significant upregulation of HLA-DR and CD86. 1-way ANOVA with repeated measures Dunett post test was used to compare to 0 ng/mL LILRA3 control. Results expressed as mean ± SD from six donors. (*p

    Journal: Retrovirology

    Article Title: TLR8 regulation of LILRA3 in monocytes is abrogated in human immunodeficiency virus infection and correlates to CD4 counts and virus loads

    doi: 10.1186/s12977-016-0248-y

    Figure Lengend Snippet: LILRA3 stimulation of cytokines and antigen presenting cells. a qPCR of LILRA3 induced gene expression of LILRA3 , IL - 6 , IL - 1A , IL - 1B and IL - 10 . Due to the different optimal concentrations of LILRA3 for various donors, the gene expression of all four donors were shown, with the corresponding mean ± SD. b Effect of LILRA3 on the antigen presentation mechanism of monocytes and B-cells. PBMCs were stimulated with varying concentrations of LILRA3 for 48 h and analysed by flow cytometry for expression of CD80, CD86, HLA-DR and HLA-ABC on monocytes (CD14 + CD33 + ) and B-cells (CD3 + CD19 + ). On monocytes, upregulation of HLA-ABC and CD80 was observed, whereas CD86 was downregulated. On B-cells, there was a slight but significant upregulation of HLA-DR and CD86. 1-way ANOVA with repeated measures Dunett post test was used to compare to 0 ng/mL LILRA3 control. Results expressed as mean ± SD from six donors. (*p

    Article Snippet: PBMC and monocyte isolation and culture PBMCs were isolated using density gradient centrifugation by layering blood onto Biocoll (Millipore) and centrifugation at 1200×g for 20 min without brake.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Flow Cytometry, Cytometry

    Fecal s-IgA antibody titers to SD1-LPS in group1 (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol) monkeys, expressed as MN with MN ± SE, after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Journal: Apmis

    Article Title: Evaluation of an intragastric challenge model for Shigella dysenteriae 1 in rhesus monkeys (Macaca mulatta) for the pre-clinical assessment of Shigella vaccine formulations

    doi: 10.1111/apm.12168

    Figure Lengend Snippet: Fecal s-IgA antibody titers to SD1-LPS in group1 (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol) monkeys, expressed as MN with MN ± SE, after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Article Snippet: Antibody-secreting cell assay Enzyme-linked immunospot (ELISPOT) assay was performed to enumerate IgA, IgG, and IgM ASCs on fresh PBMC specimens against SD1 LPS and SD1 Invaplex antigens using Multiscreen Immobilon-P filtration, 96-well plates, EMD Millipore Corporation, Billerica, MA, USA .

    Techniques:

    IgA, IgG, and IgM antibody titers against SD1-LPS in group 1 monkeys (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol), expressed as geometric mean titer (GMT) with GMT±SE after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Journal: Apmis

    Article Title: Evaluation of an intragastric challenge model for Shigella dysenteriae 1 in rhesus monkeys (Macaca mulatta) for the pre-clinical assessment of Shigella vaccine formulations

    doi: 10.1111/apm.12168

    Figure Lengend Snippet: IgA, IgG, and IgM antibody titers against SD1-LPS in group 1 monkeys (cross symbol), group 2 (open square symbol), and group 3 (closed round symbol), expressed as geometric mean titer (GMT) with GMT±SE after challenge on day 00 and re-challenge on day 31 with SD11617 strain.

    Article Snippet: Antibody-secreting cell assay Enzyme-linked immunospot (ELISPOT) assay was performed to enumerate IgA, IgG, and IgM ASCs on fresh PBMC specimens against SD1 LPS and SD1 Invaplex antigens using Multiscreen Immobilon-P filtration, 96-well plates, EMD Millipore Corporation, Billerica, MA, USA .

    Techniques:

    Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The kinase inhibitor imatinib mesylate inhibits TNF-? production in vitro and prevents TNF-dependent acute hepatic inflammation

    doi: 10.1073/pnas.0501758102

    Figure Lengend Snippet: Imatinib inhibits TNF-α production in human myeloid cells. Human PBMCs, monocytes, or macrophages were exposed to either saline or imatinib (1 h, 1 μM) followed by 3-h stimulation with 100 ng/ml LPS. ( A ) LPS-induced TNF-α levels in supernatants of all three cell types as determined by ELISA ( n = 5; * , P ≤ 0.01). ( B and C ) Imatinib dose-dependently inhibits LPS-induced TNF-α protein ( B ) ( n = 5; * , P ≤ 0.01) and TNF-α mRNA expression ( C ) ( n = 3; * , P ≤ 0.01) in human PBMCs.

    Article Snippet: Human PBMCs or CD14-selected monocytes were stimulated with LPS at a concentration of 100 ng/ml for 12 h in the presence of either solvent or the indicated kinase inhibitor ( , SB203580, PD98059, and JNK-Inhibitor II, all used in a final concentration of 35 μM and purchased from Calbiochem).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing

    Effects of the various concentrations of rHcMTF-12 on PBMC migration in vitro . Cells were treated with control buffer, pET32a protein and multiple concentrations of rHcMTF-12. The migration percentage was determined randomly. The difference between the mean values was calculated using ANOVA. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. Data are representative of three independent experiments. * P

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Effects of the various concentrations of rHcMTF-12 on PBMC migration in vitro . Cells were treated with control buffer, pET32a protein and multiple concentrations of rHcMTF-12. The migration percentage was determined randomly. The difference between the mean values was calculated using ANOVA. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. Data are representative of three independent experiments. * P

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: Migration, In Vitro, Derivative Assay

    Relative expression of multiple cytokines in goat PBMCs stimulated by rHcMTF-12. Cells were incubated with rHcMTF-12 for 48 h, and the mRNAs encoding IL-2, IL-4, IL-6, IL-10, TGF-β1 and IFN-γ were quantified by real-time PCR. The significant level was set at * P

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Relative expression of multiple cytokines in goat PBMCs stimulated by rHcMTF-12. Cells were incubated with rHcMTF-12 for 48 h, and the mRNAs encoding IL-2, IL-4, IL-6, IL-10, TGF-β1 and IFN-γ were quantified by real-time PCR. The significant level was set at * P

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction

    Decreased level of cell proliferation by rHcMTF-12 in vitro . Cells were simulated with ConA and treated with control buffer (PBS), pET32a protein and various concentrations of rHcMTF-12 protein for 72 h. A proliferation test was conducted by CCK-8 and values were measured at 450 nm wavelength (OD 450 ) using microplate spectrophotometer. Cell proliferation index was calculated by considering the OD 450 values in the controls as 100%. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. The data are expressed as the mean ± standard error (SE) of three independent experiments. ** P

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Decreased level of cell proliferation by rHcMTF-12 in vitro . Cells were simulated with ConA and treated with control buffer (PBS), pET32a protein and various concentrations of rHcMTF-12 protein for 72 h. A proliferation test was conducted by CCK-8 and values were measured at 450 nm wavelength (OD 450 ) using microplate spectrophotometer. Cell proliferation index was calculated by considering the OD 450 values in the controls as 100%. PBMC used for all replicates of distinct treatments in each experimental repetition were derived from the same goat. The data are expressed as the mean ± standard error (SE) of three independent experiments. ** P

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: In Vitro, CCK-8 Assay, Spectrophotometry, Derivative Assay

    Binding of rHcMTF-12 protein with goat PBMCs. The cells were incubated with rat sera anti-rHcMTF-12 IgG, negative rat IgG or anti-pET32a protein IgG as the primary antibody followed by Cy3-labeled goat anti-rat IgG as the secondary antibody. Red fluorescence on surface of cells showed target protein staining (Cy3) and cell nuclei were visualized by DAPI (blue). No fluorescence was observed in the PBS control or pET32 control groups. Scale-bars : 10 μm

    Journal: Parasites & Vectors

    Article Title: Identification of a novel methyltransferase-type 12 protein from Haemonchus contortus and its effects on functions of goat PBMCs

    doi: 10.1186/s13071-020-04028-y

    Figure Lengend Snippet: Binding of rHcMTF-12 protein with goat PBMCs. The cells were incubated with rat sera anti-rHcMTF-12 IgG, negative rat IgG or anti-pET32a protein IgG as the primary antibody followed by Cy3-labeled goat anti-rat IgG as the secondary antibody. Red fluorescence on surface of cells showed target protein staining (Cy3) and cell nuclei were visualized by DAPI (blue). No fluorescence was observed in the PBS control or pET32 control groups. Scale-bars : 10 μm

    Article Snippet: rHcMTF-12 promoted migration efficiency of PBMCs The change in migratory efficiency of host PBMCs in response to rHcMTF-12 was assessed by the percentile of shifted PBMCs through the Millipore polycarbonate membrane into the lower chamber.

    Techniques: Binding Assay, Incubation, Labeling, Fluorescence, Staining