Structured Review

Abcam monoclonal vegf
<t>Vimentin</t> and <t>VEGF</t> A protein in vastus lateralis muscle with endurance training. ( A , B ) Immunoblot showing the small (32 kDa) and large (45 kDa) vimentin isoforms (A) and VEGF A monomers and dimer (B) in one participant for each genotype before and after endurance training. The position of the actin band on the Ponceau-S-stained membrane before immunoblotting, which served as a loading control, is indicated. For image assembly see S5 , S6 , S7 , S8 , S9 and S10 Figs. (C, D) Box whisker plot visualizing the median ± standard error (central lines and boxes, respectively) and minima/maxima (whiskers) of the fold changes in vimentin (C) and VEGF A content (D). In total biopsies from 18 subjects were assessed: A/A ( n = 4), A/T ( n = 10), and T/T ( n = 4). *, p
Monoclonal Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal vegf/product/Abcam
Average 91 stars, based on 1 article reviews
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monoclonal vegf - by Bioz Stars, 2020-09
91/100 stars

Images

1) Product Images from "T/T homozygosity of the tenascin-C gene polymorphism rs2104772 negatively influences exercise-induced angiogenesis"

Article Title: T/T homozygosity of the tenascin-C gene polymorphism rs2104772 negatively influences exercise-induced angiogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0174864

Vimentin and VEGF A protein in vastus lateralis muscle with endurance training. ( A , B ) Immunoblot showing the small (32 kDa) and large (45 kDa) vimentin isoforms (A) and VEGF A monomers and dimer (B) in one participant for each genotype before and after endurance training. The position of the actin band on the Ponceau-S-stained membrane before immunoblotting, which served as a loading control, is indicated. For image assembly see S5 , S6 , S7 , S8 , S9 and S10 Figs. (C, D) Box whisker plot visualizing the median ± standard error (central lines and boxes, respectively) and minima/maxima (whiskers) of the fold changes in vimentin (C) and VEGF A content (D). In total biopsies from 18 subjects were assessed: A/A ( n = 4), A/T ( n = 10), and T/T ( n = 4). *, p
Figure Legend Snippet: Vimentin and VEGF A protein in vastus lateralis muscle with endurance training. ( A , B ) Immunoblot showing the small (32 kDa) and large (45 kDa) vimentin isoforms (A) and VEGF A monomers and dimer (B) in one participant for each genotype before and after endurance training. The position of the actin band on the Ponceau-S-stained membrane before immunoblotting, which served as a loading control, is indicated. For image assembly see S5 , S6 , S7 , S8 , S9 and S10 Figs. (C, D) Box whisker plot visualizing the median ± standard error (central lines and boxes, respectively) and minima/maxima (whiskers) of the fold changes in vimentin (C) and VEGF A content (D). In total biopsies from 18 subjects were assessed: A/A ( n = 4), A/T ( n = 10), and T/T ( n = 4). *, p

Techniques Used: Staining, Whisker Assay

Related Articles

Immunodetection:

Article Title: T/T homozygosity of the tenascin-C gene polymorphism rs2104772 negatively influences exercise-induced angiogenesis
Article Snippet: .. Immunodetection was performed using the monoclonal tenascin-C antibody B28.13 (a gift from Prof. Matthias Chiquet; 1:50 dilution), monoclonal vimentin antibody MAB1681 (MERCK Millipore, Schaffhausen, Switzerland, 1:1000 dilution), or monoclonal VEGF A antibody 26503 (Abcam, Cambridge, UK, 1: 500 dilution) and anti-mouse secondary horseradish peroxidase-conjugated antibody (1:5000 dilution of A-2304 or 1:20’000 dilution of A-9917 from Sigma, Buchs Switzerland). .. The bands corresponding to the tagged proteins were detected using chemoluminescence (Femto kit; Pierce, Fisher Scientific, Wohlen, Switzerland) and recorded using a Chemidoc system with Quantity One software (Bio-Rad, Hercules, CA, USA).

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  • 91
    Abcam monoclonal vegf
    <t>Vimentin</t> and <t>VEGF</t> A protein in vastus lateralis muscle with endurance training. ( A , B ) Immunoblot showing the small (32 kDa) and large (45 kDa) vimentin isoforms (A) and VEGF A monomers and dimer (B) in one participant for each genotype before and after endurance training. The position of the actin band on the Ponceau-S-stained membrane before immunoblotting, which served as a loading control, is indicated. For image assembly see S5 , S6 , S7 , S8 , S9 and S10 Figs. (C, D) Box whisker plot visualizing the median ± standard error (central lines and boxes, respectively) and minima/maxima (whiskers) of the fold changes in vimentin (C) and VEGF A content (D). In total biopsies from 18 subjects were assessed: A/A ( n = 4), A/T ( n = 10), and T/T ( n = 4). *, p
    Monoclonal Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal vegf/product/Abcam
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    monoclonal vegf - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    92
    Abcam rabbit monoclonal primary antibodies against human vegf
    Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor <t>[VEGF],</t> angiopoietin 2 <t>[Ang2],</t> tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4
    Rabbit Monoclonal Primary Antibodies Against Human Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal primary antibodies against human vegf/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal primary antibodies against human vegf - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Abcam mouse anti vegf monoclonal antibody
    Expression levels of <t>CD41,</t> BCAR1, LOX, FAK, <t>VEGF</t> and MVD in patients with carcinoma, borderline cystadenoma or cystadenoma group. (A) CD41, (B) BCAR1, (C) LOX, (D) FAK, (E) VEGF and (F) MVD staining among the different histotype groups. Histotypes were regrouped into cystadenoma, borderline and carcinoma. Bars represent the minimum and maximum values, open circles indicate outliers. CD41, platelet glycoprotein IIb; BCAR1, breast cancer anti-estrogen resistance 1; LOX, lysyl oxidase; FAK, focal adhesion kinase; VEGF, vascular endothelial growth factor; MVD, microvascular density.
    Mouse Anti Vegf Monoclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti vegf monoclonal antibody/product/Abcam
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    mouse anti vegf monoclonal antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Vimentin and VEGF A protein in vastus lateralis muscle with endurance training. ( A , B ) Immunoblot showing the small (32 kDa) and large (45 kDa) vimentin isoforms (A) and VEGF A monomers and dimer (B) in one participant for each genotype before and after endurance training. The position of the actin band on the Ponceau-S-stained membrane before immunoblotting, which served as a loading control, is indicated. For image assembly see S5 , S6 , S7 , S8 , S9 and S10 Figs. (C, D) Box whisker plot visualizing the median ± standard error (central lines and boxes, respectively) and minima/maxima (whiskers) of the fold changes in vimentin (C) and VEGF A content (D). In total biopsies from 18 subjects were assessed: A/A ( n = 4), A/T ( n = 10), and T/T ( n = 4). *, p

    Journal: PLoS ONE

    Article Title: T/T homozygosity of the tenascin-C gene polymorphism rs2104772 negatively influences exercise-induced angiogenesis

    doi: 10.1371/journal.pone.0174864

    Figure Lengend Snippet: Vimentin and VEGF A protein in vastus lateralis muscle with endurance training. ( A , B ) Immunoblot showing the small (32 kDa) and large (45 kDa) vimentin isoforms (A) and VEGF A monomers and dimer (B) in one participant for each genotype before and after endurance training. The position of the actin band on the Ponceau-S-stained membrane before immunoblotting, which served as a loading control, is indicated. For image assembly see S5 , S6 , S7 , S8 , S9 and S10 Figs. (C, D) Box whisker plot visualizing the median ± standard error (central lines and boxes, respectively) and minima/maxima (whiskers) of the fold changes in vimentin (C) and VEGF A content (D). In total biopsies from 18 subjects were assessed: A/A ( n = 4), A/T ( n = 10), and T/T ( n = 4). *, p

    Article Snippet: Immunodetection was performed using the monoclonal tenascin-C antibody B28.13 (a gift from Prof. Matthias Chiquet; 1:50 dilution), monoclonal vimentin antibody MAB1681 (MERCK Millipore, Schaffhausen, Switzerland, 1:1000 dilution), or monoclonal VEGF A antibody 26503 (Abcam, Cambridge, UK, 1: 500 dilution) and anti-mouse secondary horseradish peroxidase-conjugated antibody (1:5000 dilution of A-2304 or 1:20’000 dilution of A-9917 from Sigma, Buchs Switzerland).

    Techniques: Staining, Whisker Assay

    Dermal EB distributed along vasculatures and the perivascular spaces. The EB distribution was observed in the mystacial pad at 2.0 h-post injection and the sections from edge of injection site. (A) and (B) Evans blue diffused into the mystacial sweat glands and hair follicles. (C) EB distributed in the fasciculus and the perineurium. (D) and (E) Evans blue diffused along endothelial cells and perivascular tissues. (F) Evans blue distributed along the lymphatic vessel and blood capillary. (G–L) EB overlaid or surrounded VEGF immuno-reactivity. (M–O) EB located around the Lyve1 immuno-reactivity. White arrows: the sweat gland; green dash line: hair follicle; yellow arrows or dash circle: the sensory nerve fibers and fasciculus; blue arrows or dash circles: vasculatures and perivascular spaces or tissues; white arrowheads: the perineurium. The bar is 50 μm, (A–F) 20×, (G–O) 40×.

    Journal: Drug Delivery

    Article Title: The involvement of perivascular spaces or tissues in the facial intradermal brain-targeted delivery

    doi: 10.1080/10717544.2019.1587044

    Figure Lengend Snippet: Dermal EB distributed along vasculatures and the perivascular spaces. The EB distribution was observed in the mystacial pad at 2.0 h-post injection and the sections from edge of injection site. (A) and (B) Evans blue diffused into the mystacial sweat glands and hair follicles. (C) EB distributed in the fasciculus and the perineurium. (D) and (E) Evans blue diffused along endothelial cells and perivascular tissues. (F) Evans blue distributed along the lymphatic vessel and blood capillary. (G–L) EB overlaid or surrounded VEGF immuno-reactivity. (M–O) EB located around the Lyve1 immuno-reactivity. White arrows: the sweat gland; green dash line: hair follicle; yellow arrows or dash circle: the sensory nerve fibers and fasciculus; blue arrows or dash circles: vasculatures and perivascular spaces or tissues; white arrowheads: the perineurium. The bar is 50 μm, (A–F) 20×, (G–O) 40×.

    Article Snippet: VEGF monoclonal antibody and Lyve1 polyclonal antibody were purchased from Abcam, USA and Santa Cruz Biotechnology, USA, respectively.

    Techniques: Injection

    Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4

    Journal: Arthritis Research & Therapy

    Article Title: Oxidative stress impairs energy metabolism in primary cells and synovial tissue of patients with rheumatoid arthritis

    doi: 10.1186/s13075-018-1592-1

    Figure Lengend Snippet: Synovial tissue (ST) angiogenesis, oxidative stress and cellular bioenergetics. To support the concept that oxidative stress, angiogenesis and energy metabolism are interconnected processes that co-exist during the inflammation milieu, double-immunofluorescence staining was performed. ST slides were co-incubated with primary mouse antibody against human 4-hydroxy-2-nonenal (4-HNE) and with primary rabbit antibodies against angiogenic factors (vascular endothelial growth factor [VEGF], angiopoietin 2 [Ang2], tyrosine kinase receptor [Tie2]), glycolytic proteins (glyceraldehyde 3-phosphate dehydrogenase [GAPDH], pyruvate kinase isozyme 2 [PKM2], glucose transporter 1 [GLUT1]) and a mitochondrial marker (adenosine triphosphate synthase subunit β [ATP5B]). Representative merged immunofluorescence images demonstrate examples of co-localisation ( yellow ) of 4-HNE with VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Cells stained green are positive for 4-HNE only; cells stained red are positive only for VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B. Arrows indicate examples of co-localisation. Magnification of photomicrographs × 20, insets show high-power magnification of co-localisation. Representative images show single immunofluorescence of 4-HNE, VEGF, Ang2, Tie2, GAPDH, PKM2, GLUT1 and ATP5B along with their controls. Isotype-matched antibodies are shown in Additional file 3 : Figure S3 and Additional file 4 : Figure S4

    Article Snippet: The sections were incubated with rabbit monoclonal primary antibodies against human VEGF, Ang2, Tie2, ATP5B (all from Abcam), GAPDH (Trevigen) and mouse monoclonal antibodies against human 4-HNE (GENTAUR).

    Techniques: Double Immunofluorescence Staining, Incubation, Marker, Immunofluorescence, Staining

    Expression levels of CD41, BCAR1, LOX, FAK, VEGF and MVD in patients with carcinoma, borderline cystadenoma or cystadenoma group. (A) CD41, (B) BCAR1, (C) LOX, (D) FAK, (E) VEGF and (F) MVD staining among the different histotype groups. Histotypes were regrouped into cystadenoma, borderline and carcinoma. Bars represent the minimum and maximum values, open circles indicate outliers. CD41, platelet glycoprotein IIb; BCAR1, breast cancer anti-estrogen resistance 1; LOX, lysyl oxidase; FAK, focal adhesion kinase; VEGF, vascular endothelial growth factor; MVD, microvascular density.

    Journal: Molecular Medicine Reports

    Article Title: Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism

    doi: 10.3892/mmr.2014.3082

    Figure Lengend Snippet: Expression levels of CD41, BCAR1, LOX, FAK, VEGF and MVD in patients with carcinoma, borderline cystadenoma or cystadenoma group. (A) CD41, (B) BCAR1, (C) LOX, (D) FAK, (E) VEGF and (F) MVD staining among the different histotype groups. Histotypes were regrouped into cystadenoma, borderline and carcinoma. Bars represent the minimum and maximum values, open circles indicate outliers. CD41, platelet glycoprotein IIb; BCAR1, breast cancer anti-estrogen resistance 1; LOX, lysyl oxidase; FAK, focal adhesion kinase; VEGF, vascular endothelial growth factor; MVD, microvascular density.

    Article Snippet: The tissue sections were then incubated with rabbit anti-CD41 polyclonal antibody (1:100; ab63983), mouse anti-CD31 monoclonal antibody (1:100; ab24590) and/or mouse anti-VEGF monoclonal antibody (1:200; ab1316), which were all purchased from Abcam, overnight at 4°C.

    Techniques: Expressing, Staining

    Differences in the expression levels of CD41, BCAR1, LOX, FAK, VEGF and CD31 (MVD) in different histoypes. (A) CD41; (B) BCAR1; (C) LOX; (D) FAK; (E VEGF and (F) MVD staining among the different histotype groups. Clinical specimens from 140 patients with SC, BSC, SCC, MC, BMC, MCC and CCC were subject to analysis of immunohistochemical markers. No differences in ages were observed among the clinical categories (mean=46.1, standard deviation=17.8). Bars represent the minimum and maximum values, open circles indicate outliers. SC, serous cystadenoma; BSC, borderline serous cystadenoma; SCC, serous cystadenocarcinoma; MC, mucinous cystadenoma; BMC, borderline mucinous cystadenoma; MCC, mucinous cystadenocarcinoma; CCC, clear cell carcinoma; LOX, lysyl oxidase; FAK, focal adhesion kinase; VEGF, vascular endothelial growth factor; MVD, microvascular density.

    Journal: Molecular Medicine Reports

    Article Title: Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism

    doi: 10.3892/mmr.2014.3082

    Figure Lengend Snippet: Differences in the expression levels of CD41, BCAR1, LOX, FAK, VEGF and CD31 (MVD) in different histoypes. (A) CD41; (B) BCAR1; (C) LOX; (D) FAK; (E VEGF and (F) MVD staining among the different histotype groups. Clinical specimens from 140 patients with SC, BSC, SCC, MC, BMC, MCC and CCC were subject to analysis of immunohistochemical markers. No differences in ages were observed among the clinical categories (mean=46.1, standard deviation=17.8). Bars represent the minimum and maximum values, open circles indicate outliers. SC, serous cystadenoma; BSC, borderline serous cystadenoma; SCC, serous cystadenocarcinoma; MC, mucinous cystadenoma; BMC, borderline mucinous cystadenoma; MCC, mucinous cystadenocarcinoma; CCC, clear cell carcinoma; LOX, lysyl oxidase; FAK, focal adhesion kinase; VEGF, vascular endothelial growth factor; MVD, microvascular density.

    Article Snippet: The tissue sections were then incubated with rabbit anti-CD41 polyclonal antibody (1:100; ab63983), mouse anti-CD31 monoclonal antibody (1:100; ab24590) and/or mouse anti-VEGF monoclonal antibody (1:200; ab1316), which were all purchased from Abcam, overnight at 4°C.

    Techniques: Expressing, Staining, Countercurrent Chromatography, Immunohistochemistry, Standard Deviation

    Colocalization of the platelet marker CD41 (green) with (A) CD31 (red) or (B) VEGF (red) in human ovarian mucinous cystadenocarcinoma. Tissues (4μm) were incubated with anti-CD41, CD31 and VEFG antibodies prior to staining with fluorescein isothiocyanate and rhodamine-conjugated secondary antibodies. The nuclei were stained using diamidinophenylindole (blue). The samples were analyzed by fluorescence microscopy (magnification, 400x). CD41, platelet glycoprotein IIb;;CD31, platelet endothelial cell adhesion molecule 1; VEGF, vascular endothelial growth factor.

    Journal: Molecular Medicine Reports

    Article Title: Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism

    doi: 10.3892/mmr.2014.3082

    Figure Lengend Snippet: Colocalization of the platelet marker CD41 (green) with (A) CD31 (red) or (B) VEGF (red) in human ovarian mucinous cystadenocarcinoma. Tissues (4μm) were incubated with anti-CD41, CD31 and VEFG antibodies prior to staining with fluorescein isothiocyanate and rhodamine-conjugated secondary antibodies. The nuclei were stained using diamidinophenylindole (blue). The samples were analyzed by fluorescence microscopy (magnification, 400x). CD41, platelet glycoprotein IIb;;CD31, platelet endothelial cell adhesion molecule 1; VEGF, vascular endothelial growth factor.

    Article Snippet: The tissue sections were then incubated with rabbit anti-CD41 polyclonal antibody (1:100; ab63983), mouse anti-CD31 monoclonal antibody (1:100; ab24590) and/or mouse anti-VEGF monoclonal antibody (1:200; ab1316), which were all purchased from Abcam, overnight at 4°C.

    Techniques: Marker, Incubation, Staining, Fluorescence, Microscopy