Journal: Frontiers in Immunology

Article Title: Impact of age on liver damage, inflammation, and molecular signaling pathways in response to femoral fracture and hemorrhage

doi: 10.3389/fimmu.2023.1239145

Figure Lengend Snippet: The effect of aging on IκBα and Akt following hemorrhagic shock (trauma/hemorrhage, TH) and femoral fracture (Fx) in young (17-26 weeks) and aged (64-72 weeks) male C57BL/6J mice. The experimental design included young (17-26 weeks) and aged (64-72 weeks) C57BL/6J mice in both sham and trauma groups. The trauma groups underwent a pressure-controlled hemorrhagic shock and Fx (via osteotomy) (THFx), while the sham groups underwent catheterization and received an external fixator, but no THFx was induced. After twenty-four hours, the mice were euthanized, and sampling was performed. (A) Western blot analysis of phosphorylated and non-phosphorylated IκBα, AKT as well as β-Actin, and quantification of (B) the phosphorylation ratio of IκBα, and (C) AKT in liver tissue. n=4 in each group, *: p<0.05 between indicated groups.

Article Snippet: IκBα Rabbit polyclonal antibody (9242S, 1:1000), Phospho-IκBα Mouse mAb (9246S, 1:1000), Akt Rabbit mAb (4691S, 1:1000), and Phospho-Akt Rabbit mAb (4060S, 1: 2000, all Cell Signaling Technology) were used as primary antibodies.

Techniques: Sampling, Western Blot

Journal: iScience

Article Title: mTORC2 regulates auditory hair cell structure and function

doi: 10.1016/j.isci.2023.107687

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-phospho-Akt (Ser473) IgG , Cell Signaling Technology , Cat#4060; RRID: AB_2315049.

Techniques: Recombinant, Saline, Electron Microscopy, Software, Imaging

Journal: iScience

Article Title: Activation of AKT induces EZH2-mediated β-catenin trimethylation in colorectal cancer

doi: 10.1016/j.isci.2023.107630

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti- Phospho-Akt Substrate (RXXS∗/T∗) , Cell Signaling Technology , Cat#9614, RRID: AB_331810.

Techniques: Virus, shRNA, Plasmid Preparation, Recombinant, Protease Inhibitor, DNA Purification, Mutagenesis, Software

Journal: Cell reports

Article Title: Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells

doi: 10.1016/j.celrep.2023.112794

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-Phospho-Akt (Ser473) (D9E) XP ® , Cell Signaling Technology , Cat# 4060; RRID: AB_2315049.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay, Staining, Modification, Concentration Assay, Over Expression, Protein Extraction, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Protease Inhibitor, CCK-8 Assay, Selection, Plasmid Preparation, Knock-Out, Software, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: iScience

Article Title: Soluble epoxide hydrolase maintains steady-state lipid turnover linked with autocrine signaling in peritoneal macrophages

doi: 10.1016/j.isci.2023.107465

Figure Lengend Snippet: AHBA activated kinase signaling (A) cPLA2 phosphorylation and AHBA-induced LPA receptor activation. Total cPLA2 and its phosphorylated form were examined in TGPMs treated with the indicated inhibitors. (B) cPLA2 phosphorylation and AHBA-induced ERK activation. Total cPLA2 and its phosphorylated form were examined in TGPMs treated with the indicated inhibitors. (C) ERK phosphorylation and AHBA-induced LPA receptor activation. Total ERK and its phosphorylated form were examined in TGPMs treated with the indicated inhibitors. (D) AKT phosphorylation and AHBA-induced LPA receptor activation. Total AKT and its Thr308-and Ser473-phosphorylated forms were examined in TGPMs treated with the indicated inhibitors. The inhibitors used are the LPA receptor blocker H2L5765834, the TXA2 receptor blocker daltroban, the ERK inhibitor FR 180204, the ROCK inhibitor Y-27632, and the PI3K inhibitor TG100-116. Total cell lysates were prepared from TGPMs treated with the combination of inhibitors for 20 min and subjected to immune-blot analysis. The resulting immune-signals were captured as shown in the representative digital images and quantitatively analyzed as presented in the plot graphs. The relative abundance is related to the average level of the right-side group in each graph. The data represent means ± SEM., and the mice examined are shown in parentheses. Significant differences between the groups indicated by bars are statistically examined (ns: not significant, ∗p < 0.05 and ∗∗p < 0.01 in ANOVA and Sidak’s test).

Article Snippet: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb , Cell Signaling Technology , Cat#2965; RRID: AB_2255933.

Techniques: Activation Assay

Journal: iScience

Article Title: Soluble epoxide hydrolase maintains steady-state lipid turnover linked with autocrine signaling in peritoneal macrophages

doi: 10.1016/j.isci.2023.107465

Figure Lengend Snippet:

Article Snippet: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb , Cell Signaling Technology , Cat#2965; RRID: AB_2255933.

Techniques: Purification, Derivative Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, H2O2 Assay, Microarray, Software