Structured Review

Cell Signaling Technology Inc monoclonal rabbit anti human sp1
Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and <t>SP1</t> as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.
Monoclonal Rabbit Anti Human Sp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal rabbit anti human sp1/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monoclonal rabbit anti human sp1 - by Bioz Stars, 2020-07
86/100 stars

Images

1) Product Images from "Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas"

Article Title: Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas

Journal: Frontiers in Pediatrics

doi: 10.3389/fped.2018.00378

Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.
Figure Legend Snippet: Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.

Techniques Used: Western Blot, Expressing, Marker, Isolation

2) Product Images from "Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells"

Article Title: Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells

Journal: Oncotarget

doi:

RBP2 expression induced by CagA is Sp1 dependent (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with CagA transfection in GC cells was determined by QRT-PCR and western blot respectively. Data are mean±SD of 3 biological replicates, *: P
Figure Legend Snippet: RBP2 expression induced by CagA is Sp1 dependent (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with CagA transfection in GC cells was determined by QRT-PCR and western blot respectively. Data are mean±SD of 3 biological replicates, *: P

Techniques Used: Expressing, Transfection, Quantitative RT-PCR, Western Blot

PI3K/AKT participates in RBP2 induction by CagA through Sp1 (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with AKTi and CagA treatment solely or jointly in GC cells respectively. Data are mean±SD of 3 biological replicates, *: P
Figure Legend Snippet: PI3K/AKT participates in RBP2 induction by CagA through Sp1 (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with AKTi and CagA treatment solely or jointly in GC cells respectively. Data are mean±SD of 3 biological replicates, *: P

Techniques Used:

Co-expression of RBP2 and Sp1 expression in clinical samples from chronic inflammation to dysplasia (A) RBP2 and Sp1 expression in serial sections from tissues of clinical samples (chronic inflammation to dysplasia). Representative images are shown here. Original magnification, ×10. (B) and (C) A summary of RBP2 and Sp1 expression in the above tissues. (D) An outline of the main pathways illustrated in our work.
Figure Legend Snippet: Co-expression of RBP2 and Sp1 expression in clinical samples from chronic inflammation to dysplasia (A) RBP2 and Sp1 expression in serial sections from tissues of clinical samples (chronic inflammation to dysplasia). Representative images are shown here. Original magnification, ×10. (B) and (C) A summary of RBP2 and Sp1 expression in the above tissues. (D) An outline of the main pathways illustrated in our work.

Techniques Used: Expressing

3) Product Images from "Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas"

Article Title: Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas

Journal: Frontiers in Pediatrics

doi: 10.3389/fped.2018.00378

Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.
Figure Legend Snippet: Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.

Techniques Used: Western Blot, Expressing, Marker, Isolation

Related Articles

Incubation:

Article Title: Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells
Article Snippet: .. After antigen retrieve, H2 O2 treatment and non-specific antigens blocking, the slides were incubated with monoclonal rabbit anti-human RBP2 (Sigma, USA; 1:150) or monoclonal rabbit anti-human Sp1 (Cell Signaling, USA) or rabbit anti-human Cyclin D1 (Bioss, China) overnight at 4°C. .. Secondary antibodies were used to incubate the slides and the avidin-biotin-peroxidase method was used to detect the antibody binding with DAB staining (Vector Laboratories, Burlingame, CA, USA).

Blocking Assay:

Article Title: Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells
Article Snippet: .. After antigen retrieve, H2 O2 treatment and non-specific antigens blocking, the slides were incubated with monoclonal rabbit anti-human RBP2 (Sigma, USA; 1:150) or monoclonal rabbit anti-human Sp1 (Cell Signaling, USA) or rabbit anti-human Cyclin D1 (Bioss, China) overnight at 4°C. .. Secondary antibodies were used to incubate the slides and the avidin-biotin-peroxidase method was used to detect the antibody binding with DAB staining (Vector Laboratories, Burlingame, CA, USA).

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    Cell Signaling Technology Inc rabbit anti sp1 monoclonal antibody
    Western blot analysis of <t>Sp1</t> protein in 2DG-treated NCCIT cells. The whole cell lysates of NCCIT cells treated with 2DG for the indicated times (0, 24, 72, or 168 h) were precipitated with an anti-Sp1 antibody (D4C3), and the precipitated proteins were immunoblotted with D4C3, anti- O -GlcNAc antibodies (RL2 or CTD110.6), anti-Phosphoserine/threonine (P-Ser/Thr), anti-SUMO1, or anti-Ubiquitin. These transcriptional modifications were observed in Sp1 proteins [6] . Among these modifications, only O -GlcNAcylation was clearly detected in the D4C3 precipitates. The Sp1 levels in whole cell lysates were shown as a reference (left panel).
    Rabbit Anti Sp1 Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti sp1 monoclonal antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti sp1 monoclonal antibody - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc monoclonal rabbit anti human sp1
    Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and <t>SP1</t> as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.
    Monoclonal Rabbit Anti Human Sp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rabbit anti human sp1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal rabbit anti human sp1 - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Western blot analysis of Sp1 protein in 2DG-treated NCCIT cells. The whole cell lysates of NCCIT cells treated with 2DG for the indicated times (0, 24, 72, or 168 h) were precipitated with an anti-Sp1 antibody (D4C3), and the precipitated proteins were immunoblotted with D4C3, anti- O -GlcNAc antibodies (RL2 or CTD110.6), anti-Phosphoserine/threonine (P-Ser/Thr), anti-SUMO1, or anti-Ubiquitin. These transcriptional modifications were observed in Sp1 proteins [6] . Among these modifications, only O -GlcNAcylation was clearly detected in the D4C3 precipitates. The Sp1 levels in whole cell lysates were shown as a reference (left panel).

    Journal: Data in Brief

    Article Title: Western blot data using two distinct anti-O-GlcNAc monoclonal antibodies showing unique glycosylation status on cellular proteins under 2-deoxy-d-glucose treatment

    doi: 10.1016/j.dib.2016.12.001

    Figure Lengend Snippet: Western blot analysis of Sp1 protein in 2DG-treated NCCIT cells. The whole cell lysates of NCCIT cells treated with 2DG for the indicated times (0, 24, 72, or 168 h) were precipitated with an anti-Sp1 antibody (D4C3), and the precipitated proteins were immunoblotted with D4C3, anti- O -GlcNAc antibodies (RL2 or CTD110.6), anti-Phosphoserine/threonine (P-Ser/Thr), anti-SUMO1, or anti-Ubiquitin. These transcriptional modifications were observed in Sp1 proteins [6] . Among these modifications, only O -GlcNAcylation was clearly detected in the D4C3 precipitates. The Sp1 levels in whole cell lysates were shown as a reference (left panel).

    Article Snippet: The primary antibodies used were mouse anti-O -GlcNAc monoclonal antibodies (RL2, Thermo Fisher Scientific, Waltham, MA; CTD110.6, Cell Signaling Technology), rabbit anti-Sp1 monoclonal antibody (D4C3, Cell Signaling Technology), rabbit anti-GRP78/Bip monoclonal antibody (C50B12, Cell Signaling Technology), rabbit anti-GAPDH monoclonal antibody (D16H11, Cell Signaling Technology), rabbit anti-β-Actin polyclonal antibody (#4967, Cell Signaling Technology), rabbit anti-Phosphoserine/threonine polyclonal antibody (ab17464, abcam, Cambridge, UK), mouse anti-SUMO1 monoclonal antibody (21C7, BostonBiochem, Cambridge, MA), and mouse anti-Ubiquitin monoclonal antibody (P4D1, Cell Signaling Technology).

    Techniques: Western Blot

    Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.

    Journal: Frontiers in Pediatrics

    Article Title: Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas

    doi: 10.3389/fped.2018.00378

    Figure Lengend Snippet: Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.

    Article Snippet: For analysis of the subcellular localization, the following antibodies were used: monoclonal rabbit anti-human β-catenin (Cell Signaling Technology (CST), Danvers, MA, USA), non-phospho (active) monoclonal rabbit anti-human β-catenin (CST), monoclonal rabbit anti-human SP1 (CST), and monoclonal mouse anti-human GAPH.

    Techniques: Western Blot, Expressing, Marker, Isolation

    RBP2 expression induced by CagA is Sp1 dependent (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with CagA transfection in GC cells was determined by QRT-PCR and western blot respectively. Data are mean±SD of 3 biological replicates, *: P

    Journal: Oncotarget

    Article Title: Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells

    doi:

    Figure Lengend Snippet: RBP2 expression induced by CagA is Sp1 dependent (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with CagA transfection in GC cells was determined by QRT-PCR and western blot respectively. Data are mean±SD of 3 biological replicates, *: P

    Article Snippet: After antigen retrieve, H2 O2 treatment and non-specific antigens blocking, the slides were incubated with monoclonal rabbit anti-human RBP2 (Sigma, USA; 1:150) or monoclonal rabbit anti-human Sp1 (Cell Signaling, USA) or rabbit anti-human Cyclin D1 (Bioss, China) overnight at 4°C.

    Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

    PI3K/AKT participates in RBP2 induction by CagA through Sp1 (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with AKTi and CagA treatment solely or jointly in GC cells respectively. Data are mean±SD of 3 biological replicates, *: P

    Journal: Oncotarget

    Article Title: Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells

    doi:

    Figure Lengend Snippet: PI3K/AKT participates in RBP2 induction by CagA through Sp1 (A) and (B) RBP2 and Sp1 changes at RNA level and protein level with AKTi and CagA treatment solely or jointly in GC cells respectively. Data are mean±SD of 3 biological replicates, *: P

    Article Snippet: After antigen retrieve, H2 O2 treatment and non-specific antigens blocking, the slides were incubated with monoclonal rabbit anti-human RBP2 (Sigma, USA; 1:150) or monoclonal rabbit anti-human Sp1 (Cell Signaling, USA) or rabbit anti-human Cyclin D1 (Bioss, China) overnight at 4°C.

    Techniques:

    Co-expression of RBP2 and Sp1 expression in clinical samples from chronic inflammation to dysplasia (A) RBP2 and Sp1 expression in serial sections from tissues of clinical samples (chronic inflammation to dysplasia). Representative images are shown here. Original magnification, ×10. (B) and (C) A summary of RBP2 and Sp1 expression in the above tissues. (D) An outline of the main pathways illustrated in our work.

    Journal: Oncotarget

    Article Title: Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells

    doi:

    Figure Lengend Snippet: Co-expression of RBP2 and Sp1 expression in clinical samples from chronic inflammation to dysplasia (A) RBP2 and Sp1 expression in serial sections from tissues of clinical samples (chronic inflammation to dysplasia). Representative images are shown here. Original magnification, ×10. (B) and (C) A summary of RBP2 and Sp1 expression in the above tissues. (D) An outline of the main pathways illustrated in our work.

    Article Snippet: After antigen retrieve, H2 O2 treatment and non-specific antigens blocking, the slides were incubated with monoclonal rabbit anti-human RBP2 (Sigma, USA; 1:150) or monoclonal rabbit anti-human Sp1 (Cell Signaling, USA) or rabbit anti-human Cyclin D1 (Bioss, China) overnight at 4°C.

    Techniques: Expressing

    Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.

    Journal: Frontiers in Pediatrics

    Article Title: Canonical WNT/β-Catenin Signaling Plays a Subordinate Role in Rhabdomyosarcomas

    doi: 10.3389/fped.2018.00378

    Figure Lengend Snippet: Western Blot analysis of β-catenin expression in RMS tumor cell lines. (A) Western Blot analysis of ß-catenin expression in various RMS tumor cell lines. ß-actin served as loading control. (B) Western Blot analysis of ß-catenin expression in three different RMS tumor cell lines after 48 h treatment with either FH535, XAV939, ß-catenin siRNA, or (C) WNT3A treatment. ß-actin served as loading control. (D) Densitometric quantification of the Western Blots shown in (C) using ImageJ. Expression of ß-catenin after treatment (+) was normalized to ß-actin and compared to untreated cells (−). (E) subcellular localization of ß-catenin in the RMS tumor cell lines with and without WNT3A stimulation. GAPDH served as cytoplasmatic and SP1 as nuclear marker to check purity of the protein isolation from different compartments. CP, cytoplasmatic; N, nuclear.

    Article Snippet: For analysis of the subcellular localization, the following antibodies were used: monoclonal rabbit anti-human β-catenin (Cell Signaling Technology (CST), Danvers, MA, USA), non-phospho (active) monoclonal rabbit anti-human β-catenin (CST), monoclonal rabbit anti-human SP1 (CST), and monoclonal mouse anti-human GAPH.

    Techniques: Western Blot, Expressing, Marker, Isolation