monoclonal mouse anti human c5b 9  (Agilent technologies)

Journal: Medical Microbiology and Immunology

Article Title: Lipopolysaccharide with long O-antigen is crucial for Salmonella Enteritidis to evade complement activity and to facilitate bacterial survival in vivo in the Galleria mellonella infection model

doi: 10.1007/s00430-024-00790-3

Figure Lengend Snippet: C5b-9 deposition and the effect of complement attack on the cell membrane integrity of S . Enteritidis PCM 2817 WT and mutant strains. A Mean fluorescence intensity (MFI) of C5b-9 neo-epitope deposition on bacterial cells. B Frequency of 7AAD positive cells; the percentage of 7AAD positive cells stands for the percentage of cells with compromised membrane integrity. The experiment was performed in three biological replicates. Data was analyzed by ordinary one-way ANOVA and Dunnett's post hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001), assuming WT as the control group. Average from six measurements ± SD

Article Snippet: Cells were incubated with the monoclonal mouse anti-human C5b-9 antibody (clone aE11) (Hycult) for 45 min at ambient temperature, washed and detected with an anti-mouse Alexa 488 antibody (Abcam) (45 min, ambient temperature).

Techniques: Membrane, Mutagenesis, Fluorescence, Control