Journal: Viruses

Article Title: SARS-CoV-2, Placental Histopathology, Gravity of Infection and Immunopathology: Is There an Association?

doi: 10.3390/v14061330

Figure Lengend Snippet: Examples of immunostaining with anti-ACE2 antibody in chorionic discs from the CIC ( A ), CNIC ( B ) and control ( C ) groups (Immunohistochemistry, Original Magnification: 20× ( A – C ). (scale bar: 400 µm).

Article Snippet: Finally, we used the anti-ACE2-Receptor: mouse monoclonal Ab (mAb), code ab89111, (Abcam, Cambridge, UK), dilution 1:250 [ ].

Techniques: Immunostaining, Immunohistochemistry

Journal: Viruses

Article Title: SARS-CoV-2, Placental Histopathology, Gravity of Infection and Immunopathology: Is There an Association?

doi: 10.3390/v14061330

Figure Lengend Snippet: CD4 ( A ), CD8 ( B ), CD68 ( C ) and ACE2 ( D ) expression values in the COVID Int. Care (13 patients), COVID not Int. Care (16 patients) and Controls (28 patients).

Article Snippet: Finally, we used the anti-ACE2-Receptor: mouse monoclonal Ab (mAb), code ab89111, (Abcam, Cambridge, UK), dilution 1:250 [ ].

Techniques: Expressing

Journal: Human Vaccines & Immunotherapeutics

Article Title: Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19

doi: 10.1080/21645515.2022.2048622

Figure Lengend Snippet: Human ACE2 detection in the lungs of HIS-DRAGA mice.

Article Snippet: The membrane was blocked overnight at 4°C with gentle shaking in 5% nonfat milk plus 3% BSA in PBS, then incubated with a mouse monoclonal anti-human ACE2 (Abcam, cat#Ab89111) for 2 h at room temperature, and washed with PBS +.01% Tween 20.

Techniques:

Journal: Human Vaccines & Immunotherapeutics

Article Title: Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19

doi: 10.1080/21645515.2022.2048622

Figure Lengend Snippet: Quantification of hACE2 protein in HIS-DRAGA lungs.

Article Snippet: The membrane was blocked overnight at 4°C with gentle shaking in 5% nonfat milk plus 3% BSA in PBS, then incubated with a mouse monoclonal anti-human ACE2 (Abcam, cat#Ab89111) for 2 h at room temperature, and washed with PBS +.01% Tween 20.

Techniques:

Journal: Human Vaccines & Immunotherapeutics

Article Title: Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19

doi: 10.1080/21645515.2022.2048622

Figure Lengend Snippet: Co-Localization of hACE2 with hCD326 on alveolar human ECs in HIS-DRAGA lungs.

Article Snippet: The membrane was blocked overnight at 4°C with gentle shaking in 5% nonfat milk plus 3% BSA in PBS, then incubated with a mouse monoclonal anti-human ACE2 (Abcam, cat#Ab89111) for 2 h at room temperature, and washed with PBS +.01% Tween 20.

Techniques:

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Micrographs of ACE2, Furin and TMPRSS2 protein expression in the small airways of smokers and COPD patients. The top inset images depict small airway epithelium taken at x40 magnification. The bottom inset images depict the parenchymal alveoli at x20 magnification.

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Percent small airway (SA) epithelial expression of ACE2, Furin and TMPRSS2 in normal controls, normal lung function smokers, SAD, and COPD patients. An increase in ACE2, Furin and TMPRSS2 expression was observed in all pathological groups NLFS, SAD, COPD-CS, and COPD-ES compared to NC for ( A ) ACE2, ( B ) Furin, and ( C ) TMPRSS2. Significance is indicated as: *p <0.05, **p< 0.01, ***p<0.001 and ****p<0.0001, the one-way ANOVA test was used for statistical analysis.

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Differences in ACE2, Furin and TMPRSS2 positive and negative type 2 pneumocytes (T2P) in normal controls, normal lung function smokers, SAD, and COPD patients. ( A ) Differential inter and intragroup analysis of protein expression in type 2 pneumocytes as fold change for positive cells over negative cells in NC, NLFS, SAD, COPD-CS and COPD-ES for ( A ) ACE2, ( B ) Furin, and ( C ) TMPRSS2. Significance is indicated between intergroup *p <0.05, **p< 0.01, ***p<0.001 and intragroup ## p< 0.01, ### p<0.001 and #### p<0.0001 variability, the two-way ANOVA test was used for statistical analysis.

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Differences in ACE2, Furin and TMPRSS2 positive and negative alveolar macrophages (AMs) in normal controls, normal lung function smokers, SAD, and COPD patients. ( A ) Differential inter and intra group analysis of protein expression in alveolar macrophages as fold change for positive cells over negative cells in NC, NLFS, SAD, COPD-CS and COPD-ES for ( A ) ACE2, ( B ) Furin, and ( C ) TMPRSS2. Significance is indicated as: *p <0.05, **p< 0.01, ***p<0.001 and ****p<0.0001 and intragroup # p <0.05, ## p< 0.01 and ### p<0.001 variability, the two-way ANOVA test was used for statistical analysis.

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Correlation coefficient analysis for ACE2, Furin and TMPRSS2 in small airway epithelium, type 2 pneumocytes (T2P) and alveolar macrophages (AMs). Pearson r’ correlation analysis was performed to estimate the degree of co-expression of ACE2, Furin and TMPRSS2 in NLFS, SAD, COPD-CS and COPD-ES compared to NC in ( A ) small airway epithelium, ( B ) type 2 pneumocytes, and ( C ) alveolar macrophages. Significance is indicated as **p< 0.01 and ***p<0.001.

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Correlations of ACE2, Furin and TMPRSS2 with Physiological Lung Parameters

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Expressing

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis

doi: 10.2147/COPD.S329783

Figure Lengend Snippet: Immunofluorescence of proteins (ACE2, Furin, and TMPRSS2) associated with SARS-CoV-2 adhesion and infection. ACE2, Furin and TMPRSS2 protein expression of small airway epithelial cells following cigarette smoke exposure. Small airway epithelial cells were exposed to 1% cigarette smoke extract (CSE) or appropriate vehicle control (VC) (1% DMSO) and incubated for 24 hours. ( A ) Protein expression of ACE2 (green), Furin (red, top) and TMPRSS2 (red, bottom) with DAPI (blue) was assessed through immunofluorescence staining and confocal imaging. Images at 40×. ( B ) Cigarette smoke extract-treated cells had significantly greater corrected total cell fluorescence of ACE-2, Furin, and TMPRSS2. ( C ) Co-localisation does not vary between cigarette smoke extract-treated cells and vehicle controls (VC). Corrected total cell fluorescence and Pearson’s coefficient were calculated using ImageJ. Results are represented as medians ± range. Ns= non-significant; *p<0.05; ****p<0.0001.

Article Snippet: After blocking with a mix of 10% goat and donkey serum in PBS for a half-hour, the cells were stained with mouse monoclonal anti-human ACE2 antibody (ab89111, AbCam, Melbourne, Victoria, Australia) 1:100 and rabbit polyclonal anti-human Furin (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100 or TMPRSS2 (Thermofisher Cat#bs13228R, Bioss antibodies, VIC, Australia) 1:100, incubated overnight at 4°C.

Techniques: Immunofluorescence, Infection, Expressing, Incubation, Staining, Imaging, Fluorescence

Journal: Apoptosis

Article Title: COVID-19 disrupts spermatogenesis through the oxidative stress pathway following induction of apoptosis

doi: 10.1007/s10495-021-01680-2

Figure Lengend Snippet: Photomicrograph of the testis immunohistochemistry staining for ACE2 (4X and 40X). A Control group and B COVID-19 groups. Expressed of ACE2 in Leydig cells (arrows) according to immunohistochemistry. C Mean ± SD of the genes expression in the different groups (***P < 0.001)

Article Snippet: The primary antibodies used included monoclonal mouse anti-human ACE2 (1:100; MM0073- 11A31, Abcam, Cambridge, UK).

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Apoptosis

Article Title: COVID-19 disrupts spermatogenesis through the oxidative stress pathway following induction of apoptosis

doi: 10.1007/s10495-021-01680-2

Figure Lengend Snippet: A ROS production and level of GSH in testis tissue. Mean ± SD of the ROS production and GPX activity level in the study groups; (*P < 0.05). B Real-time-PCR analyses. mRNA expression levels of (ACE2, TMPRSS2, BAX, BCL2 and Caspase3) in the testis tissue. Mean ± SD of the genes expression in the different groups (*P < 0.05 and ***P < 0.001). PCR, polymerase chain reaction

Article Snippet: The primary antibodies used included monoclonal mouse anti-human ACE2 (1:100; MM0073- 11A31, Abcam, Cambridge, UK).

Techniques: Activity Assay, Real-time Polymerase Chain Reaction, Expressing, Polymerase Chain Reaction

Journal: Zoological Research

Article Title: Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model

doi: 10.24272/j.issn.2095-8137.2020.118

Figure Lengend Snippet: Characterization of in-vitro transfection of hACE2 and SARS-CoV-2 infection in MLE-12 cells

Article Snippet: After washing three times with PBS plus 0.05% Tween 20 (PBST), the tissue slides were permeabilized with 0.1% Triton-X100 for 15 min and labeled with mouse anti-viral nucleoprotein monoclonal antibody (40143-MM05; Sino Biological, China) or mouse anti-human ACE2 monoclonal antibody (ab89111; Abcam, UK) for 2 h at room temperature.

Techniques: In Vitro, Transfection, Infection

Journal: Zoological Research

Article Title: Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model

doi: 10.24272/j.issn.2095-8137.2020.118

Figure Lengend Snippet: In vivo hACE2 pulmonary transfection in mice

Article Snippet: After washing three times with PBS plus 0.05% Tween 20 (PBST), the tissue slides were permeabilized with 0.1% Triton-X100 for 15 min and labeled with mouse anti-viral nucleoprotein monoclonal antibody (40143-MM05; Sino Biological, China) or mouse anti-human ACE2 monoclonal antibody (ab89111; Abcam, UK) for 2 h at room temperature.

Techniques: In Vivo, Transfection

Journal: Zoological Research

Article Title: Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model

doi: 10.24272/j.issn.2095-8137.2020.118

Figure Lengend Snippet: Pulmonary SARS-CoV-2 infection in hACE2-transfected mice

Article Snippet: After washing three times with PBS plus 0.05% Tween 20 (PBST), the tissue slides were permeabilized with 0.1% Triton-X100 for 15 min and labeled with mouse anti-viral nucleoprotein monoclonal antibody (40143-MM05; Sino Biological, China) or mouse anti-human ACE2 monoclonal antibody (ab89111; Abcam, UK) for 2 h at room temperature.

Techniques: Infection, Transfection

Journal: Zoological Research

Article Title: Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model

doi: 10.24272/j.issn.2095-8137.2020.118

Figure Lengend Snippet: Comparison of SARS-CoV-2 infection in hACE2-transfected WT mice and CXCL5 −/− mice

Article Snippet: After washing three times with PBS plus 0.05% Tween 20 (PBST), the tissue slides were permeabilized with 0.1% Triton-X100 for 15 min and labeled with mouse anti-viral nucleoprotein monoclonal antibody (40143-MM05; Sino Biological, China) or mouse anti-human ACE2 monoclonal antibody (ab89111; Abcam, UK) for 2 h at room temperature.

Techniques: Infection, Transfection