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hamster monoclonal anti mouse podoplanin (AngioBio Inc)

Name: hamster monoclonal anti mouse podoplanin
Brand: AngioBio Inc
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AngioBio Inc hamster monoclonal anti mouse podoplanin
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Hamster Monoclonal Anti Mouse Podoplanin, supplied by AngioBio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hamster monoclonal anti mouse podoplanin - by Bioz Stars, 2024-12

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1) Product Images from "Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide"

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

Journal: PLoS ONE

doi: 10.1371/journal.pone.0097165

Figure Legend Snippet: Sequence of primers.

Techniques Used: Sequencing

Mouse and human leukocyte cell line J774A.1 and HL60 were immunostained with both anti-PECAM-1 (CD31) and anti-TLR2, and nuclei were stained by DAPI. In the mouse heart section, lymphatic vessels (arrowheads) were immunostained by anti-podoplanin (PDPN) and a blood vessel (arrows) was stained by anti-VE-cadherin (VE-cad). In the human kidney section with type II diabetic nephropathy, there are glomerular endothelial cells immunostained with both anti-PECAM-1 (CD31) and anti-TLR2 (arrows), and cells that are only immunostained with anti-TLR2 (arrowheads). Bar: 20 µm.

Figure Legend Snippet: Mouse and human leukocyte cell line J774A.1 and HL60 were immunostained with both anti-PECAM-1 (CD31) and anti-TLR2, and nuclei were stained by DAPI. In the mouse heart section, lymphatic vessels (arrowheads) were immunostained by anti-podoplanin (PDPN) and a blood vessel (arrows) was stained by anti-VE-cadherin (VE-cad). In the human kidney section with type II diabetic nephropathy, there are glomerular endothelial cells immunostained with both anti-PECAM-1 (CD31) and anti-TLR2 (arrows), and cells that are only immunostained with anti-TLR2 (arrowheads). Bar: 20 µm.

Techniques Used: Staining

The immunostained sections were re-stained by HE staining. The HE staining shows that renal tubules are expanded in the cortex of the kidneys of STZ-induced type I diabetic ICR mice (ICR-STZ) and of HF-induced type II diabetic KK/Ta mice (KK/Ta-HF). In the non-diabetic ICR mouse kidney section, all of the glomeruli were immunostained with the antibody for the podocyte marker, podoplanin (PDPN, arrows, staining red), while there were no cells reacting with anti-TLR2. In the ICR-STZ and KK/Ta-HF mouse kidney sections, there are areas immunostained by anti-TLR2 (arrowheads, staining green) in all of the podoplanin-positive glomeruli. In the KK/Ta mouse kidney sections, podoplanin-negative proximal tubules which are more strongly stained with eosin than the distal tubules are also immunostained by anti-TLR2 (white arrowheads, staining green). In the ICR-STZ and KK/Ta-HF mouse kidney sections, distal tubules, collecting tubules, and blood vessels outside glomeruli are not stained. Bar: 100 µm.

Figure Legend Snippet: The immunostained sections were re-stained by HE staining. The HE staining shows that renal tubules are expanded in the cortex of the kidneys of STZ-induced type I diabetic ICR mice (ICR-STZ) and of HF-induced type II diabetic KK/Ta mice (KK/Ta-HF). In the non-diabetic ICR mouse kidney section, all of the glomeruli were immunostained with the antibody for the podocyte marker, podoplanin (PDPN, arrows, staining red), while there were no cells reacting with anti-TLR2. In the ICR-STZ and KK/Ta-HF mouse kidney sections, there are areas immunostained by anti-TLR2 (arrowheads, staining green) in all of the podoplanin-positive glomeruli. In the KK/Ta mouse kidney sections, podoplanin-negative proximal tubules which are more strongly stained with eosin than the distal tubules are also immunostained by anti-TLR2 (white arrowheads, staining green). In the ICR-STZ and KK/Ta-HF mouse kidney sections, distal tubules, collecting tubules, and blood vessels outside glomeruli are not stained. Bar: 100 µm.

Techniques Used: Staining, Marker

The HE staining showed that glomeruli (Gl) of the diabetic mice are subject to sclerosis. In the laser-scanning confocal microscopy, the region reacting with anti-podoplanin (PDPN, arrowhead) does not coincide with the region reacting with anti-TLR2 (arrows) in the merged image while the regions reacting with VE-cadherin and anti-PECAM-1 (arrowheads) coincide with the regions reacting with anti-TLR2 (arrows) in the merged images (rightmost column). Bar: 20 µm.

Figure Legend Snippet: The HE staining showed that glomeruli (Gl) of the diabetic mice are subject to sclerosis. In the laser-scanning confocal microscopy, the region reacting with anti-podoplanin (PDPN, arrowhead) does not coincide with the region reacting with anti-TLR2 (arrows) in the merged image while the regions reacting with VE-cadherin and anti-PECAM-1 (arrowheads) coincide with the regions reacting with anti-TLR2 (arrows) in the merged images (rightmost column). Bar: 20 µm.

Techniques Used: Staining, Confocal Microscopy

The HE staining showed that glomeruli (Gl) of the KK/Ta-HF mice are subject to sclerosis. In the laser-scanning confocal microscopy, epithelial cells of proximal tubules (PT) did not react with anti-podoplanin (PDPN) but reacted with anti-TLR2 (arrows) at the inside. The regions reacting with VE-cadherin and anti-PECAM-1 (arrowheads) coincided with the regions reacting with anti-TLR2 (arrows) in the merged images. Bar: 20 µm.

Figure Legend Snippet: The HE staining showed that glomeruli (Gl) of the KK/Ta-HF mice are subject to sclerosis. In the laser-scanning confocal microscopy, epithelial cells of proximal tubules (PT) did not react with anti-podoplanin (PDPN) but reacted with anti-TLR2 (arrows) at the inside. The regions reacting with VE-cadherin and anti-PECAM-1 (arrowheads) coincided with the regions reacting with anti-TLR2 (arrows) in the merged images. Bar: 20 µm.

Techniques Used: Staining, Confocal Microscopy

(A) Immunohistochemistry for the expression of type I collagen and podoplanin (PDPN, red) on glomeruli by laser-scanning confocal microscopy. The intensity of the immunostaining for type I collagen is stronger in the glomeruli of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) than in the diabetic mice without LPS (STZ) and in the non-diabetic ICR mice (ICR). Bar, 20 µm. (B) Quantitative analysis for type I collagen positive areas in the glomeruli. Areas immunostained by anti-type I collagen and anti-podoplanin in ten different glomeruli of laser-scanning microscopic images were measured by ImageJ. The relative volume of type I collagen accumulation in the glomeruli was expressed by a mean ratio: area of type I collagen in a glomerulus/area of a glomerulus within podoplanin-positive podocytes. There were statistically significant differences in the relative accumulations of type I collagen in the glomeruli of non-diabetic ICR mice (ICR) and STZ-induced type I diabetic ICR mice (STZ), and between the diabetic mice and the diabetic mice with LPS (STZ+LPS). Data are expressed as means+SD. *Significantly different ( p <0.01).

Figure Legend Snippet: (A) Immunohistochemistry for the expression of type I collagen and podoplanin (PDPN, red) on glomeruli by laser-scanning confocal microscopy. The intensity of the immunostaining for type I collagen is stronger in the glomeruli of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) than in the diabetic mice without LPS (STZ) and in the non-diabetic ICR mice (ICR). Bar, 20 µm. (B) Quantitative analysis for type I collagen positive areas in the glomeruli. Areas immunostained by anti-type I collagen and anti-podoplanin in ten different glomeruli of laser-scanning microscopic images were measured by ImageJ. The relative volume of type I collagen accumulation in the glomeruli was expressed by a mean ratio: area of type I collagen in a glomerulus/area of a glomerulus within podoplanin-positive podocytes. There were statistically significant differences in the relative accumulations of type I collagen in the glomeruli of non-diabetic ICR mice (ICR) and STZ-induced type I diabetic ICR mice (STZ), and between the diabetic mice and the diabetic mice with LPS (STZ+LPS). Data are expressed as means+SD. *Significantly different ( p <0.01).

Techniques Used: Immunohistochemistry, Expressing, Confocal Microscopy, Immunostaining

(A) Immunohistochemistry for the expression of cytokines and podoplanin (PDPN) in glomeruli by laser-scanning confocal microscopy. The IL-6, TNF-α, and TGF-β were detected in the glomeruli of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administration of Porphyromonas gingivalis LPS (STZ+LPS) whereas the no cytokines were detected in the kidneys of the diabetic mice without LPS (STZ). Bar: 20 µm. (B) Tissue RT-PCR for cytokine mRNAs in mouse kidneys. The amplicons of IL-6, TNF-α, and TGF-β mRNAs were detected from both the renal cortex of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) and cultured mouse macrophage J774.1 with LPS, whereas they were not detected from the diabetic mice without LPS (STZ). (C) Tissue real-time PCR for cytokine mRNAs in mouse kidneys. The gene expression amounts of IL-6, TNF-α, and TGF-β were statistically significantly larger in the kidney tissue of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) than in the diabetic mice without LPS (STZ) and in the non-diabetic ICR mice with LPS (LPS). Data are expressed as means+SD. *Significantly different ( p <0.01).

Figure Legend Snippet: (A) Immunohistochemistry for the expression of cytokines and podoplanin (PDPN) in glomeruli by laser-scanning confocal microscopy. The IL-6, TNF-α, and TGF-β were detected in the glomeruli of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administration of Porphyromonas gingivalis LPS (STZ+LPS) whereas the no cytokines were detected in the kidneys of the diabetic mice without LPS (STZ). Bar: 20 µm. (B) Tissue RT-PCR for cytokine mRNAs in mouse kidneys. The amplicons of IL-6, TNF-α, and TGF-β mRNAs were detected from both the renal cortex of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) and cultured mouse macrophage J774.1 with LPS, whereas they were not detected from the diabetic mice without LPS (STZ). (C) Tissue real-time PCR for cytokine mRNAs in mouse kidneys. The gene expression amounts of IL-6, TNF-α, and TGF-β were statistically significantly larger in the kidney tissue of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) than in the diabetic mice without LPS (STZ) and in the non-diabetic ICR mice with LPS (LPS). Data are expressed as means+SD. *Significantly different ( p <0.01).

Techniques Used: Immunohistochemistry, Expressing, Confocal Microscopy, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Real-time Polymerase Chain Reaction

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Journal: Cell Reports Medicine

Article Title: Multimodal immune phenotyping reveals microbial-T cell interactions that shape pancreatic cancer

doi: 10.1016/j.xcrm.2024.101397

Figure Lengend Snippet:

Article Snippet: Hamster monoclonal anti-mouse podoplanin (8.1.1) , BioLegend , 127401; RRID:AB_108918.

Techniques: Recombinant, Sequencing, Software

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: Sequence of primers.

Article Snippet: The cells and sections were fixed in 100% methanol for 5 min at −20°C, treated with 0.1% goat serum for 30 min at 20°C, and then treated for 8 hrs at 4°C with PBS containing 0.1% goat serum and the following primary antibodies (1 µg/ml): hamster monoclonal anti-mouse podoplanin (AngioBio Co., Del Mar, CA) as a podocyte marker, rat monoclonal anti-mouse TLR2 (R&D Systems Inc., Minneapolis, MN), rabbit polyclonal anti-mouse vascular endothelial (VE)-cadherin (Abcam plc., Cambridge, UK), rabbit polyclonal anti-mouse platelet endothelial cell adhesion molecule-1 (PECAM-1, Abcam), rabbit polyclonal anti-mouse IL-6 (Abcam), rabbit polyclonal anti-mouse TNF-α (Abcam), rabbit polyclonal anti-mouse TGF-β (Abcam), and rabbit polyclonal anti-type I collagen (Abcam).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: Mouse and human leukocyte cell line J774A.1 and HL60 were immunostained with both anti-PECAM-1 (CD31) and anti-TLR2, and nuclei were stained by DAPI. In the mouse heart section, lymphatic vessels (arrowheads) were immunostained by anti-podoplanin (PDPN) and a blood vessel (arrows) was stained by anti-VE-cadherin (VE-cad). In the human kidney section with type II diabetic nephropathy, there are glomerular endothelial cells immunostained with both anti-PECAM-1 (CD31) and anti-TLR2 (arrows), and cells that are only immunostained with anti-TLR2 (arrowheads). Bar: 20 µm.

Techniques: Staining

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: The immunostained sections were re-stained by HE staining. The HE staining shows that renal tubules are expanded in the cortex of the kidneys of STZ-induced type I diabetic ICR mice (ICR-STZ) and of HF-induced type II diabetic KK/Ta mice (KK/Ta-HF). In the non-diabetic ICR mouse kidney section, all of the glomeruli were immunostained with the antibody for the podocyte marker, podoplanin (PDPN, arrows, staining red), while there were no cells reacting with anti-TLR2. In the ICR-STZ and KK/Ta-HF mouse kidney sections, there are areas immunostained by anti-TLR2 (arrowheads, staining green) in all of the podoplanin-positive glomeruli. In the KK/Ta mouse kidney sections, podoplanin-negative proximal tubules which are more strongly stained with eosin than the distal tubules are also immunostained by anti-TLR2 (white arrowheads, staining green). In the ICR-STZ and KK/Ta-HF mouse kidney sections, distal tubules, collecting tubules, and blood vessels outside glomeruli are not stained. Bar: 100 µm.

Techniques: Staining, Marker

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: The HE staining showed that glomeruli (Gl) of the diabetic mice are subject to sclerosis. In the laser-scanning confocal microscopy, the region reacting with anti-podoplanin (PDPN, arrowhead) does not coincide with the region reacting with anti-TLR2 (arrows) in the merged image while the regions reacting with VE-cadherin and anti-PECAM-1 (arrowheads) coincide with the regions reacting with anti-TLR2 (arrows) in the merged images (rightmost column). Bar: 20 µm.

Techniques: Staining, Confocal Microscopy

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: The HE staining showed that glomeruli (Gl) of the KK/Ta-HF mice are subject to sclerosis. In the laser-scanning confocal microscopy, epithelial cells of proximal tubules (PT) did not react with anti-podoplanin (PDPN) but reacted with anti-TLR2 (arrows) at the inside. The regions reacting with VE-cadherin and anti-PECAM-1 (arrowheads) coincided with the regions reacting with anti-TLR2 (arrows) in the merged images. Bar: 20 µm.

Techniques: Staining, Confocal Microscopy

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: (A) Immunohistochemistry for the expression of type I collagen and podoplanin (PDPN, red) on glomeruli by laser-scanning confocal microscopy. The intensity of the immunostaining for type I collagen is stronger in the glomeruli of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) than in the diabetic mice without LPS (STZ) and in the non-diabetic ICR mice (ICR). Bar, 20 µm. (B) Quantitative analysis for type I collagen positive areas in the glomeruli. Areas immunostained by anti-type I collagen and anti-podoplanin in ten different glomeruli of laser-scanning microscopic images were measured by ImageJ. The relative volume of type I collagen accumulation in the glomeruli was expressed by a mean ratio: area of type I collagen in a glomerulus/area of a glomerulus within podoplanin-positive podocytes. There were statistically significant differences in the relative accumulations of type I collagen in the glomeruli of non-diabetic ICR mice (ICR) and STZ-induced type I diabetic ICR mice (STZ), and between the diabetic mice and the diabetic mice with LPS (STZ+LPS). Data are expressed as means+SD. *Significantly different ( p <0.01).

Techniques: Immunohistochemistry, Expressing, Confocal Microscopy, Immunostaining

Journal: PLoS ONE

Article Title: Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide

doi: 10.1371/journal.pone.0097165

Figure Lengend Snippet: (A) Immunohistochemistry for the expression of cytokines and podoplanin (PDPN) in glomeruli by laser-scanning confocal microscopy. The IL-6, TNF-α, and TGF-β were detected in the glomeruli of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administration of Porphyromonas gingivalis LPS (STZ+LPS) whereas the no cytokines were detected in the kidneys of the diabetic mice without LPS (STZ). Bar: 20 µm. (B) Tissue RT-PCR for cytokine mRNAs in mouse kidneys. The amplicons of IL-6, TNF-α, and TGF-β mRNAs were detected from both the renal cortex of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) and cultured mouse macrophage J774.1 with LPS, whereas they were not detected from the diabetic mice without LPS (STZ). (C) Tissue real-time PCR for cytokine mRNAs in mouse kidneys. The gene expression amounts of IL-6, TNF-α, and TGF-β were statistically significantly larger in the kidney tissue of STZ-induced type I diabetic ICR mice with the repeated intraperitoneal administrations of Porphyromonas gingivalis LPS (STZ+LPS) than in the diabetic mice without LPS (STZ) and in the non-diabetic ICR mice with LPS (LPS). Data are expressed as means+SD. *Significantly different ( p <0.01).

Techniques: Immunohistochemistry, Expressing, Confocal Microscopy, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Real-time Polymerase Chain Reaction

Immunohistochemical staining of Lewis tumor. Notes: ( A ) LYVE-1-positive staining and ( B ) podoplanin-positive staining were seen in the Lewis tumor. Anti-podoplanin antibody could also stain a large number of other cells besides the lymphatic vessels (arrows), but anti-LYVE-1 antibody identified mainly only lymphatic vessels (arrows). Bar = 100 μm. Abbreviation: LYVE-1, lymphatic vessel endothelial hyaluronan receptor-1.

Journal: International Journal of Nanomedicine

Article Title: Mouse lymphatic endothelial cell targeted probes: anti-LYVE-1 antibody-based magnetic nanoparticles

doi: 10.2147/IJN.S45817

Figure Lengend Snippet: Immunohistochemical staining of Lewis tumor. Notes: ( A ) LYVE-1-positive staining and ( B ) podoplanin-positive staining were seen in the Lewis tumor. Anti-podoplanin antibody could also stain a large number of other cells besides the lymphatic vessels (arrows), but anti-LYVE-1 antibody identified mainly only lymphatic vessels (arrows). Bar = 100 μm. Abbreviation: LYVE-1, lymphatic vessel endothelial hyaluronan receptor-1.

Article Snippet: The following primary antibodies were used: hamster monoclonal anti-mouse podoplanin antibody (1:200, ab11936; Abcam, Cambridge, UK), rat monoclonal anti-mouse LYVE-1 antibody (1:100, MAB2125; R&D SYSTEMS Inc, Minneapolis, MN, USA), biotinylated anti-hamster immunoglobulin G (IgG) (1:100, ab6782; Abcam), and biotinylated anti-rat IgG (1:100, ZB2307; ZSGB-BIO, Beijing, People’s Republic of China).

Techniques: Immunohistochemical staining, Staining

DHJST increases lymphangiogenesis in ankle joints of TNF-Tg mice. (a) Three-month DHJST or saline treatment, ankle joints were harvested and subjected to double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies. Representative LYVE-1 (green) and podoplanin (red) stained ankle sections show that LYVE-1+/podoplanin+ lymphatic vessels (white arrows) are present at synovium and soft tissue surrounding the ankle joints of WT and TNF-Tg mice. (b) Quantitation of LYVE-1+/podoplanin+ lymphatic vessel area inside the areas of ankle joint. Values are the means ± SD of 5-6 legs per group. ∗ p < 0.05 versus WT group, # p < 0.05 versus saline treated TNF-Tg group.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Du-Huo-Ji-Sheng-Tang Attenuates Inflammation of TNF-Tg Mice Related to Promoting Lymphatic Drainage Function

doi: 10.1155/2016/7067691

Figure Lengend Snippet: DHJST increases lymphangiogenesis in ankle joints of TNF-Tg mice. (a) Three-month DHJST or saline treatment, ankle joints were harvested and subjected to double immunofluorescence staining with anti-LYVE-1 and anti-podoplanin antibodies. Representative LYVE-1 (green) and podoplanin (red) stained ankle sections show that LYVE-1+/podoplanin+ lymphatic vessels (white arrows) are present at synovium and soft tissue surrounding the ankle joints of WT and TNF-Tg mice. (b) Quantitation of LYVE-1+/podoplanin+ lymphatic vessel area inside the areas of ankle joint. Values are the means ± SD of 5-6 legs per group. ∗ p < 0.05 versus WT group, # p < 0.05 versus saline treated TNF-Tg group.

Article Snippet: Briefly, after antigen retrieval with 20 μ g/mL proteinase K, sections were blocked in 5% BSA-PBST for 30 min and then incubated with hamster monoclonal anti-mouse podoplanin (Abcam Inc., Cambridge, MA; cat. #ab11936; clone: 8.1.1, 1 : 1,000) and rabbit anti-mouse lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1, Abcam Inc., Cambridge, MA, USA; cat. #ab14917; lot.

Techniques: Double Immunofluorescence Staining, Staining, Quantitation Assay

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1) Product Images from "Expression of Toll-Like Receptor 2 in Glomerular Endothelial Cells and Promotion of Diabetic Nephropathy by Porphyromonas gingivalis Lipopolysaccharide"

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