monoclonal antibodies against cacybp sip  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc monoclonal antibodies against cacybp sip
    Monoclonal Antibodies Against Cacybp Sip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

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    Structured Review

    Millipore monoclonal antibodies against cacybp sip
    Monoclonal Antibodies Against Cacybp Sip, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images


    Structured Review

    Millipore monoclonal antibodies against cacybp sip
    Monoclonal Antibodies Against Cacybp Sip, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images

    monoclonal antibodies against cacybp sip  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 86

    Structured Review

    Cell Signaling Technology Inc monoclonal antibodies against cacybp sip
    Monoclonal Antibodies Against Cacybp Sip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images

    monoclonal antibodies against cacybp sip  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 86

    Structured Review

    Cell Signaling Technology Inc monoclonal antibodies against cacybp sip
    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. <t>CacyBP/SIP:</t> Calcyclin binding protein/Siah-1 interacting protein.
    Monoclonal Antibodies Against Cacybp Sip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation"

    Article Title: CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.v20.i29.10062

    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Western Blot, Binding Assay, Expressing

    Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).
    Figure Legend Snippet: Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay

    Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection

    Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.
    Figure Legend Snippet: Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay, Expressing

    monoclonal antibodies against cacybp sip  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • Team
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  • 86

    Structured Review

    Cell Signaling Technology Inc monoclonal antibodies against cacybp sip
    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. <t>CacyBP/SIP:</t> Calcyclin binding protein/Siah-1 interacting protein.
    Monoclonal Antibodies Against Cacybp Sip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation"

    Article Title: CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.v20.i29.10062

    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Western Blot, Binding Assay, Expressing

    Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).
    Figure Legend Snippet: Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay

    Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection

    Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.
    Figure Legend Snippet: Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay, Expressing


    Structured Review

    Millipore monoclonal antibodies against cacybp sip
    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. <t>CacyBP/SIP:</t> Calcyclin binding protein/Siah-1 interacting protein.
    Monoclonal Antibodies Against Cacybp Sip, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation"

    Article Title: CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.v20.i29.10062

    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Western Blot, Binding Assay, Expressing

    Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).
    Figure Legend Snippet: Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay

    Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection

    Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.
    Figure Legend Snippet: Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay, Expressing


    Structured Review

    Millipore monoclonal antibodies against cacybp sip
    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. <t>CacyBP/SIP:</t> Calcyclin binding protein/Siah-1 interacting protein.
    Monoclonal Antibodies Against Cacybp Sip, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation"

    Article Title: CacyBP/SIP nuclear translocation induced by gastrin promotes gastric cancer cell proliferation

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.v20.i29.10062

    Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Western blot analysis of calcyclin binding protein/Siah-1 interacting protein expression in gastric cancer cell lines. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Western Blot, Binding Assay, Expressing

    Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Gastrin can stimulate calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured gastric cancer cells. a, b and c, unstimulated cells; d, e and f, cells stimulated with gastrin (10-8 mol/L) for 8 h; a and d, immunostained using CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. C: Cytosolic markers; N: Nuclear markers; PI: Propidium iodide; PARP: Poly ADP-ribose polymerase; CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).
    Figure Legend Snippet: Calcyclin binding protein/Siah-1 interacting nuclear translocation is able to promote proliferation of gastric cancer cells. A: Effects of calcyclin binding protein/Siah-1 interacting protein (CacyBP/SIP) nuclear translocation on the proliferation of SGC7901 and MKN45 cells. As described in Materials and Methods, the cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: CacyBP/SIP nuclear translocation results in enhanced anchorage-dependent growth. Colony formation in SGC7901 and MKN45 cells with or without 10-8 mol/L gastrin. aP < 0.05 vs control (without gastrin).

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay

    Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Expression of calcyclin binding protein/Siah-1 interacting protein is evaluated by Western blot in cells stably transfected with calcyclin binding protein/Siah-1 interacting protein siRNAs. β-actin was used as an internal control. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection

    Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.
    Figure Legend Snippet: Localization of calcyclin binding protein/Siah-1 interacting protein nuclear translocation. A: Immunofluorescent localization of CacyBP/SIP in cultured SGC7901-CacyBP/SIPsi1 cells. a, b and c, unstimulated cells; d, e and f, cells 8 h after gastrin (10-8 mol/L) stimulation; a and d, immunostained using anti-CacyBP/SIP MAb; b and e, PI stained; c and f, merged images; B: Amounts of CacyBP/SIP in nuclear extracts are shown before and after gastrin (10-8 mol/L) stimulation. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein; C: Cytosolic markers; N: Nuclear markers.

    Techniques Used: Binding Assay, Translocation Assay, Cell Culture, Staining

    Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.
    Figure Legend Snippet: Inhibiting calcyclin binding protein/Siah-1 interacting protein nuclear translocation blocks proliferation of gastric cancer cells. A: Effects of gastrin on the viability of SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells. The cells were treated with gastrin (10-6-10-10 mol/L) for 24 or 48 h, and their viability was determined by MTT assay. Columns, means; bars, mean ± SD of three separate experiments in which each treatment was done in 5 wells; B: Inhibiting CacyBP/SIP expression resulted in the decrease of anchorage-dependent growth and colony formation in SGC7901-CacyBP/SIPsi1 and MKN45-CacyBP/SIPsi1 cells with or without 10-8 mol/L gastrin. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.

    Techniques Used: Binding Assay, Translocation Assay, MTT Assay, Expressing


    Structured Review

    Abcam monoclonal antibodies against cacybp sip
    The amino acid sequence of mouse <t>CacyBP/SIP</t> (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)
    Monoclonal Antibodies Against Cacybp Sip, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Abcam
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    1) Product Images from "The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells"

    Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

    Journal: Neurochemical Research

    doi: 10.1007/s11064-013-1155-4

    The amino acid sequence of mouse CacyBP/SIP (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)
    Figure Legend Snippet: The amino acid sequence of mouse CacyBP/SIP (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)

    Techniques Used: Sequencing

    Co-immunoprecipitation of CacyBP/SIP with Ubc9 in NB2a cell extract. Cells were co-transfected with pGW1-HA-Ubc9 and pCMV3xFLAG-CacyBP/SIP (designated as HA-Ubc9 and 3xFLAG-CacyBP/SIP) or pGW1-HA-Ubc9 and pCMV3xFLAG (designated as HA-Ubc9). Proteins from the extract were used directly for Western blot analysis (80 μg) or incubated with anti-FLAG M2 affinity resin (800 μg). Proteins co-precipitated with CacyBP/SIP-3xFLAG were examined by Western blot using anti-Ubc9 or anti-CacyBP/SIP antibodies. Western blot images representative for 3 experiments performed are shown
    Figure Legend Snippet: Co-immunoprecipitation of CacyBP/SIP with Ubc9 in NB2a cell extract. Cells were co-transfected with pGW1-HA-Ubc9 and pCMV3xFLAG-CacyBP/SIP (designated as HA-Ubc9 and 3xFLAG-CacyBP/SIP) or pGW1-HA-Ubc9 and pCMV3xFLAG (designated as HA-Ubc9). Proteins from the extract were used directly for Western blot analysis (80 μg) or incubated with anti-FLAG M2 affinity resin (800 μg). Proteins co-precipitated with CacyBP/SIP-3xFLAG were examined by Western blot using anti-Ubc9 or anti-CacyBP/SIP antibodies. Western blot images representative for 3 experiments performed are shown

    Techniques Used: Immunoprecipitation, Transfection, Western Blot, Incubation

    Sumoylation of CacyBP/SIP in neuroblastoma NB2a cells. Western blot developed with anti-CacyBP/SIP antibody shows sumoylated CacyBP/SIP in cell extract ( A ) and in subcellular fractions of NB2a cells ( B ). In each case 80 μg of protein was applied on the gel. Panel C shows that wild type CacyBP/SIP (WT) is sumoylated in NB2a cell extract while K16R CacyBP/SIP mutant is not. Western blot images representative for 3 experiments performed are shown
    Figure Legend Snippet: Sumoylation of CacyBP/SIP in neuroblastoma NB2a cells. Western blot developed with anti-CacyBP/SIP antibody shows sumoylated CacyBP/SIP in cell extract ( A ) and in subcellular fractions of NB2a cells ( B ). In each case 80 μg of protein was applied on the gel. Panel C shows that wild type CacyBP/SIP (WT) is sumoylated in NB2a cell extract while K16R CacyBP/SIP mutant is not. Western blot images representative for 3 experiments performed are shown

    Techniques Used: Western Blot, Mutagenesis

    Model of the CacyBP/SIP N-terminal fragment (NTF) with bound SUMO1. The NTF structure of mouse CacyBP/SIP was taken from the Protein Data Bank (PDB; id:1YSM) as the first model of the 20 NMR structures . The structure of human SUMO1, which shares 100 % amino acid sequence identity with the mouse ortholog, was extracted from a crystal structure of the human SUMO E1 complex (PDB; id:3KYC) . Both proteins were bonded via residues K16 from NTF of CacyBP/SIP and G97 from the SUMO1 C-terminus. The K16 residue of CacyBP/SIP ( red ) and SUMO1 molecule are shown in semitransparent surfaces (Color figure online)
    Figure Legend Snippet: Model of the CacyBP/SIP N-terminal fragment (NTF) with bound SUMO1. The NTF structure of mouse CacyBP/SIP was taken from the Protein Data Bank (PDB; id:1YSM) as the first model of the 20 NMR structures . The structure of human SUMO1, which shares 100 % amino acid sequence identity with the mouse ortholog, was extracted from a crystal structure of the human SUMO E1 complex (PDB; id:3KYC) . Both proteins were bonded via residues K16 from NTF of CacyBP/SIP and G97 from the SUMO1 C-terminus. The K16 residue of CacyBP/SIP ( red ) and SUMO1 molecule are shown in semitransparent surfaces (Color figure online)

    Techniques Used: Sequencing


    Structured Review

    Abcam monoclonal antibodies against cacybp sip
    Monoclonal Antibodies Against Cacybp Sip, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
    86/100 stars

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    Cell Signaling Technology Inc monoclonal antibodies against cacybp sip
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    Abcam monoclonal antibodies against cacybp sip
    The amino acid sequence of mouse <t>CacyBP/SIP</t> (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)
    Monoclonal Antibodies Against Cacybp Sip, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cacybp sip/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cacybp sip - by Bioz Stars, 2024-07
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    The amino acid sequence of mouse CacyBP/SIP (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)

    Journal: Neurochemical Research

    Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

    doi: 10.1007/s11064-013-1155-4

    Figure Lengend Snippet: The amino acid sequence of mouse CacyBP/SIP (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)

    Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

    Techniques: Sequencing

    Co-immunoprecipitation of CacyBP/SIP with Ubc9 in NB2a cell extract. Cells were co-transfected with pGW1-HA-Ubc9 and pCMV3xFLAG-CacyBP/SIP (designated as HA-Ubc9 and 3xFLAG-CacyBP/SIP) or pGW1-HA-Ubc9 and pCMV3xFLAG (designated as HA-Ubc9). Proteins from the extract were used directly for Western blot analysis (80 μg) or incubated with anti-FLAG M2 affinity resin (800 μg). Proteins co-precipitated with CacyBP/SIP-3xFLAG were examined by Western blot using anti-Ubc9 or anti-CacyBP/SIP antibodies. Western blot images representative for 3 experiments performed are shown

    Journal: Neurochemical Research

    Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

    doi: 10.1007/s11064-013-1155-4

    Figure Lengend Snippet: Co-immunoprecipitation of CacyBP/SIP with Ubc9 in NB2a cell extract. Cells were co-transfected with pGW1-HA-Ubc9 and pCMV3xFLAG-CacyBP/SIP (designated as HA-Ubc9 and 3xFLAG-CacyBP/SIP) or pGW1-HA-Ubc9 and pCMV3xFLAG (designated as HA-Ubc9). Proteins from the extract were used directly for Western blot analysis (80 μg) or incubated with anti-FLAG M2 affinity resin (800 μg). Proteins co-precipitated with CacyBP/SIP-3xFLAG were examined by Western blot using anti-Ubc9 or anti-CacyBP/SIP antibodies. Western blot images representative for 3 experiments performed are shown

    Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

    Techniques: Immunoprecipitation, Transfection, Western Blot, Incubation

    Sumoylation of CacyBP/SIP in neuroblastoma NB2a cells. Western blot developed with anti-CacyBP/SIP antibody shows sumoylated CacyBP/SIP in cell extract ( A ) and in subcellular fractions of NB2a cells ( B ). In each case 80 μg of protein was applied on the gel. Panel C shows that wild type CacyBP/SIP (WT) is sumoylated in NB2a cell extract while K16R CacyBP/SIP mutant is not. Western blot images representative for 3 experiments performed are shown

    Journal: Neurochemical Research

    Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

    doi: 10.1007/s11064-013-1155-4

    Figure Lengend Snippet: Sumoylation of CacyBP/SIP in neuroblastoma NB2a cells. Western blot developed with anti-CacyBP/SIP antibody shows sumoylated CacyBP/SIP in cell extract ( A ) and in subcellular fractions of NB2a cells ( B ). In each case 80 μg of protein was applied on the gel. Panel C shows that wild type CacyBP/SIP (WT) is sumoylated in NB2a cell extract while K16R CacyBP/SIP mutant is not. Western blot images representative for 3 experiments performed are shown

    Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

    Techniques: Western Blot, Mutagenesis

    Model of the CacyBP/SIP N-terminal fragment (NTF) with bound SUMO1. The NTF structure of mouse CacyBP/SIP was taken from the Protein Data Bank (PDB; id:1YSM) as the first model of the 20 NMR structures . The structure of human SUMO1, which shares 100 % amino acid sequence identity with the mouse ortholog, was extracted from a crystal structure of the human SUMO E1 complex (PDB; id:3KYC) . Both proteins were bonded via residues K16 from NTF of CacyBP/SIP and G97 from the SUMO1 C-terminus. The K16 residue of CacyBP/SIP ( red ) and SUMO1 molecule are shown in semitransparent surfaces (Color figure online)

    Journal: Neurochemical Research

    Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

    doi: 10.1007/s11064-013-1155-4

    Figure Lengend Snippet: Model of the CacyBP/SIP N-terminal fragment (NTF) with bound SUMO1. The NTF structure of mouse CacyBP/SIP was taken from the Protein Data Bank (PDB; id:1YSM) as the first model of the 20 NMR structures . The structure of human SUMO1, which shares 100 % amino acid sequence identity with the mouse ortholog, was extracted from a crystal structure of the human SUMO E1 complex (PDB; id:3KYC) . Both proteins were bonded via residues K16 from NTF of CacyBP/SIP and G97 from the SUMO1 C-terminus. The K16 residue of CacyBP/SIP ( red ) and SUMO1 molecule are shown in semitransparent surfaces (Color figure online)

    Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

    Techniques: Sequencing