monoclonal antibodies against cacybp sip  (Abcam)

Journal: Neurochemical Research

Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

doi: 10.1007/s11064-013-1155-4

Figure Lengend Snippet: The amino acid sequence of mouse CacyBP/SIP (accession number NP_033916). Lysine residues, identified by SUMOsp 2.0 tool, which might be potentially sumoylated are marked in bold (these with high probability) or underlined (these with lower probability)

Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

Techniques: Sequencing

Journal: Neurochemical Research

Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

doi: 10.1007/s11064-013-1155-4

Figure Lengend Snippet: Co-immunoprecipitation of CacyBP/SIP with Ubc9 in NB2a cell extract. Cells were co-transfected with pGW1-HA-Ubc9 and pCMV3xFLAG-CacyBP/SIP (designated as HA-Ubc9 and 3xFLAG-CacyBP/SIP) or pGW1-HA-Ubc9 and pCMV3xFLAG (designated as HA-Ubc9). Proteins from the extract were used directly for Western blot analysis (80 μg) or incubated with anti-FLAG M2 affinity resin (800 μg). Proteins co-precipitated with CacyBP/SIP-3xFLAG were examined by Western blot using anti-Ubc9 or anti-CacyBP/SIP antibodies. Western blot images representative for 3 experiments performed are shown

Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

Techniques: Immunoprecipitation, Transfection, Western Blot, Incubation

Journal: Neurochemical Research

Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

doi: 10.1007/s11064-013-1155-4

Figure Lengend Snippet: Sumoylation of CacyBP/SIP in neuroblastoma NB2a cells. Western blot developed with anti-CacyBP/SIP antibody shows sumoylated CacyBP/SIP in cell extract ( A ) and in subcellular fractions of NB2a cells ( B ). In each case 80 μg of protein was applied on the gel. Panel C shows that wild type CacyBP/SIP (WT) is sumoylated in NB2a cell extract while K16R CacyBP/SIP mutant is not. Western blot images representative for 3 experiments performed are shown

Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

Techniques: Western Blot, Mutagenesis

Journal: Neurochemical Research

Article Title: The CacyBP/SIP Protein is Sumoylated in Neuroblastoma NB2a Cells

doi: 10.1007/s11064-013-1155-4

Figure Lengend Snippet: Model of the CacyBP/SIP N-terminal fragment (NTF) with bound SUMO1. The NTF structure of mouse CacyBP/SIP was taken from the Protein Data Bank (PDB; id:1YSM) as the first model of the 20 NMR structures . The structure of human SUMO1, which shares 100 % amino acid sequence identity with the mouse ortholog, was extracted from a crystal structure of the human SUMO E1 complex (PDB; id:3KYC) . Both proteins were bonded via residues K16 from NTF of CacyBP/SIP and G97 from the SUMO1 C-terminus. The K16 residue of CacyBP/SIP ( red ) and SUMO1 molecule are shown in semitransparent surfaces (Color figure online)

Article Snippet: Proteins from the cell extract (80 μg) were analyzed by Western blot using monoclonal antibodies against CacyBP/SIP (Abcam).

Techniques: Sequencing