monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh  (Merck KGaA)

 
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    Structured Review

    Merck KGaA monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh
    Monoclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Merck KGaA
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Protein Concentration:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Protein concentration was quantified using a Pierce BCA Protein Assay Kit (bicinchoninic acid) and absorbance was measured at 562 nm using a Nanodrop 1000 (Thermo Fisher Scientific Inc.). .. These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: Protein concentration was evaluated with the bicinchoninic acid technique (Pierce BCA Protein Assay Kit), absorbance at 562 nm is measured using nanodrop 1000 (Thermo Fisher Scientific Inc. Waltham, MA, USA) Equal amounts of proteins (40 µg) were loaded onto 10% Tis-Glycine gel (CriterionTM XT precast gel) using XT Tricine running Buffer (Biorad, Hercules, CA, USA). .. After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Blocking Assay:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: .. These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore). .. Membranes were washed with TBS and 0.1% Tween 20 and then incubated with 1:10,000 IR-Dye 800-conjugated secondary donkey anti-rabbit or IR-Dye 700-conjugated secondary anti-mouse antibodies (LI-COR Biosciences) in blocking buffer with 0.1% Tween 20 and 0.1% SDS (LI-COR Biosciences).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: .. After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore). .. Blots were washed with TBS-T buffer [20 mM tris (pH 7.4), 150 mm NaCl, and 0.05% Tween 20] and incubated with 1:10,000 IR-Dye 800-conjugated secondary donkey anti-goat or IR-Dye 700-conjugated secondary anti-mouse antibodies (LI-COR Biosciences) in blocking buffer added with 0.1% Tween 20 (LI-COR Biosciences, Lincoln, NE, USA).

    Electrophoresis:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in TBS for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies. .. These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: .. After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore). .. Blots were washed with TBS-T buffer [20 mM tris (pH 7.4), 150 mm NaCl, and 0.05% Tween 20] and incubated with 1:10,000 IR-Dye 800-conjugated secondary donkey anti-goat or IR-Dye 700-conjugated secondary anti-mouse antibodies (LI-COR Biosciences) in blocking buffer added with 0.1% Tween 20 (LI-COR Biosciences, Lincoln, NE, USA).

    Incubation:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in TBS for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies. .. These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: .. After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore). .. Blots were washed with TBS-T buffer [20 mM tris (pH 7.4), 150 mm NaCl, and 0.05% Tween 20] and incubated with 1:10,000 IR-Dye 800-conjugated secondary donkey anti-goat or IR-Dye 700-conjugated secondary anti-mouse antibodies (LI-COR Biosciences) in blocking buffer added with 0.1% Tween 20 (LI-COR Biosciences, Lincoln, NE, USA).

    Imaging:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore). .. Odyssey Infrared Imaging System (LI-COR Biosciences) was used to reveal the membranes.

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore). .. For IR-Dye 800 and IR-Dye 700 detection, an Odyssey Infrared Imaging System (LI-COR Biosciences) was used.

    BIA-KA:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Protein concentration was quantified using a Pierce BCA Protein Assay Kit (bicinchoninic acid) and absorbance was measured at 562 nm using a Nanodrop 1000 (Thermo Fisher Scientific Inc.). .. These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: Protein concentration was evaluated with the bicinchoninic acid technique (Pierce BCA Protein Assay Kit), absorbance at 562 nm is measured using nanodrop 1000 (Thermo Fisher Scientific Inc. Waltham, MA, USA) Equal amounts of proteins (40 µg) were loaded onto 10% Tis-Glycine gel (CriterionTM XT precast gel) using XT Tricine running Buffer (Biorad, Hercules, CA, USA). .. After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Western Blot:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Paragraph title: 2.6. Western Blotting ... These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: Paragraph title: 2.5. Western-Blot ... After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Lysis:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Western Blotting Total fibroblast proteins were extracted in 200 μL of NP40 Cell Lysis buffer (Invitrogen, Carlsbad, CA, USA) with Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific). .. These primary antibodies were diluted in blocking buffer with 0.1% Tween 20, 1:1000 rabbit polyclonal anti-lamin A/C (sc-20681, Santa Cruz Biotechnology) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

    Article Title: Antisense-Based Progerin Downregulation in HGPS-Like Patients’ Cells
    Article Snippet: Western-Blot Total fibroblast proteins were extracted in 200 μL of NP40 Cell Lysis buffer (Invitrogen, Carlsbad, CA, USA) containing Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific Inc. Waltham, MA, USA). .. After electrophoresis, gels were electro transferred onto nitrocellulose membranes or Immobilon-FL polyvinylidene fluoride membranes (Millipore), blocked in Odyssey Blocking Buffer diluted 1:1 in PBS for 1 h at room temperature, and incubated overnight at 4 °C or 2 h at room temperature with various primary antibodies diluted in blocking buffer added with 0.1% Tween 20: 1:1000 rabbit polyclonal anti-Lamin A/C (sc-20681, Santa Cruz Biotechnology, Dallas, TX, USA), 1:200 goat polyclonal anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), 1:1000 mouse monoclonal anti-Progerin (ab66587, Abcam, Cambridge, UK), 1:1000 mouse monoclonal anti-actin (MAB1501, Merck Millipore, Darmstadt, Germany) and 1:40,000 monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MAB374, Merck Millipore).

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  • 91
    Merck KGaA mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
    Expression levels and subcellular localization of glucokinase. (a) Effects of Src downregulation on expression level of glucokinase messenger ribonucleic acid (mRNA). Expression level of glucokinase was evaluated with semiquantitative real‐time polymerase chain reaction. Data were normalized using β‐actin mRNA ( n = 5 in each group). Values are expressed as mean ± standard error of the mean. (b) Effects of Src downregulation on expression level of glucokinase protein. Lysates of whole INS‐1 cells were used for immunoblotting analysis with antibodies against glucokinase. Quantification data were obtained from four independent experiments and normalized with β‐actin level. Values are expressed as mean ± standard error of the mean. (c) Effects of Src downregulation on subcellular localization of glucokinase. Lysates of whole INS‐1 cells were separated to soluble fraction and pellet. Both fractions were used for immunoblotting analysis with antibodies against glucokinase. Quantification data for each fraction were obtained from four independent experiments and normalized with glyceraldehyde <t>3‐phosphate</t> dehydrogenase <t>(GAPDH)</t> levels for soluble fraction and calnexin levels for pellet. Values are expressed as mean ± standard error of the mean. * P
    Mouse Monoclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody/product/Merck KGaA
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    94
    Merck KGaA glyceraldehyde 3 phosphate dehydrogenase
    Expression levels and subcellular localization of glucokinase. (a) Effects of Src downregulation on expression level of glucokinase messenger ribonucleic acid (mRNA). Expression level of glucokinase was evaluated with semiquantitative real‐time polymerase chain reaction. Data were normalized using β‐actin mRNA ( n = 5 in each group). Values are expressed as mean ± standard error of the mean. (b) Effects of Src downregulation on expression level of glucokinase protein. Lysates of whole INS‐1 cells were used for immunoblotting analysis with antibodies against glucokinase. Quantification data were obtained from four independent experiments and normalized with β‐actin level. Values are expressed as mean ± standard error of the mean. (c) Effects of Src downregulation on subcellular localization of glucokinase. Lysates of whole INS‐1 cells were separated to soluble fraction and pellet. Both fractions were used for immunoblotting analysis with antibodies against glucokinase. Quantification data for each fraction were obtained from four independent experiments and normalized with glyceraldehyde <t>3‐phosphate</t> dehydrogenase <t>(GAPDH)</t> levels for soluble fraction and calnexin levels for pellet. Values are expressed as mean ± standard error of the mean. * P
    Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase/product/Merck KGaA
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Expression levels and subcellular localization of glucokinase. (a) Effects of Src downregulation on expression level of glucokinase messenger ribonucleic acid (mRNA). Expression level of glucokinase was evaluated with semiquantitative real‐time polymerase chain reaction. Data were normalized using β‐actin mRNA ( n = 5 in each group). Values are expressed as mean ± standard error of the mean. (b) Effects of Src downregulation on expression level of glucokinase protein. Lysates of whole INS‐1 cells were used for immunoblotting analysis with antibodies against glucokinase. Quantification data were obtained from four independent experiments and normalized with β‐actin level. Values are expressed as mean ± standard error of the mean. (c) Effects of Src downregulation on subcellular localization of glucokinase. Lysates of whole INS‐1 cells were separated to soluble fraction and pellet. Both fractions were used for immunoblotting analysis with antibodies against glucokinase. Quantification data for each fraction were obtained from four independent experiments and normalized with glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) levels for soluble fraction and calnexin levels for pellet. Values are expressed as mean ± standard error of the mean. * P

    Journal: Journal of Diabetes Investigation

    Article Title: Src regulates insulin secretion and glucose metabolism by influencing subcellular localization of glucokinase in pancreatic β‐cells

    doi: 10.1111/jdi.12407

    Figure Lengend Snippet: Expression levels and subcellular localization of glucokinase. (a) Effects of Src downregulation on expression level of glucokinase messenger ribonucleic acid (mRNA). Expression level of glucokinase was evaluated with semiquantitative real‐time polymerase chain reaction. Data were normalized using β‐actin mRNA ( n = 5 in each group). Values are expressed as mean ± standard error of the mean. (b) Effects of Src downregulation on expression level of glucokinase protein. Lysates of whole INS‐1 cells were used for immunoblotting analysis with antibodies against glucokinase. Quantification data were obtained from four independent experiments and normalized with β‐actin level. Values are expressed as mean ± standard error of the mean. (c) Effects of Src downregulation on subcellular localization of glucokinase. Lysates of whole INS‐1 cells were separated to soluble fraction and pellet. Both fractions were used for immunoblotting analysis with antibodies against glucokinase. Quantification data for each fraction were obtained from four independent experiments and normalized with glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) levels for soluble fraction and calnexin levels for pellet. Values are expressed as mean ± standard error of the mean. * P

    Article Snippet: Primary antibodies used were mouse monoclonal anti‐Src antibody from Merck KGaA (Darmstadt, Germany); mouse monoclonal anti‐complex I (39 kDa subunit), anti‐complex III (core II), anti‐complex IV (subunit I) and anti‐complex V (subunit α) of mitochondrial respiratory chain antibody from Invitrogen (Eugene, OR, USA); rabbit anti‐glucokinase antibody and anti‐GLUT2 antibody from Abcam (Cambridge, UK); rabbit anti‐Lyn, anti‐Lck, anti‐Fgr, anti‐Blk, anti‐C‐terminal Src kinase (Csk), anti‐calnexin and goat anti‐Hck antibody from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit anti‐neuronal nitric oxide synthase (nNOS) antibody from Cell Signaling Technology (Danvers, MA, USA); mouse monoclonal anti‐β‐actin antibody from Sigma‐Aldrich; and mouse monoclonal anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibody from Merck KGaA.

    Techniques: Expressing, Real-time Polymerase Chain Reaction