monoclonal anti beta actin (Millipore)
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Name:
Monoclonal Anti beta Actin antibody
Description:
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types It varies in amount being related to the type of differentiation and to the functional state of cells and tissues Actin can be found in two different forms of aggregation the globular or the fibrillar state and at least six distinct isoforms occur in vertebrates The actins exhibit over 90 sequence homology but each isoform has a unique NH2 terminal sequence The isoforms are comprised of three α actins skeletal cardiac smooth one β actin β non muscle and two γ actins γ smooth muscle and γ non muscle ACTB actin β gene codes for β actin It is a cytoskeletal housekeeping protein It is present in the cytoplasm The ACTB gene is located on human chromosome 7p22 1 Monoclonal Anti β Actin mouse IgG2a isotype is derived from the AC 74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse
Catalog Number:
a5316
Price:
None
Applications:
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.
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Structured Review
Millipore
monoclonal anti beta actin
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types It varies in amount being related to the type of differentiation and to the functional state of cells and tissues Actin can be found in two different forms of aggregation the globular or the fibrillar state and at least six distinct isoforms occur in vertebrates The actins exhibit over 90 sequence homology but each isoform has a unique NH2 terminal sequence The isoforms are comprised of three α actins skeletal cardiac smooth one β actin β non muscle and two γ actins γ smooth muscle and γ non muscle ACTB actin β gene codes for β actin It is a cytoskeletal housekeeping protein It is present in the cytoplasm The ACTB gene is located on human chromosome 7p22 1 Monoclonal Anti β Actin mouse IgG2a isotype is derived from the AC 74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse
https://www.bioz.com/result/monoclonal anti beta actin/product/Millipore
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types It varies in amount being related to the type of differentiation and to the functional state of cells and tissues Actin can be found in two different forms of aggregation the globular or the fibrillar state and at least six distinct isoforms occur in vertebrates The actins exhibit over 90 sequence homology but each isoform has a unique NH2 terminal sequence The isoforms are comprised of three α actins skeletal cardiac smooth one β actin β non muscle and two γ actins γ smooth muscle and γ non muscle ACTB actin β gene codes for β actin It is a cytoskeletal housekeeping protein It is present in the cytoplasm The ACTB gene is located on human chromosome 7p22 1 Monoclonal Anti β Actin mouse IgG2a isotype is derived from the AC 74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse
https://www.bioz.com/result/monoclonal anti beta actin/product/Millipore
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
monoclonal anti beta actin - by Bioz Stars,
2020-01
90/100 stars
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Images
1) Product Images from "Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites"
Article Title: Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
Journal: International Journal of Nanomedicine
doi: 10.2147/IJN.S56661
Figure Legend Snippet: Concentrations (percentage of PS) of beta-actin ( A ), vinculin ( B ), talin ( C ). osteocalcin ( D ), osteopontin ( E ), and ICAM ( F ) in MG-63 cells growing on composite material (0), composite materials with 2, 5,10, 15, and 25 vol% of nano-sized tricalcium phosphate (N2–N25) or of micro-sized tricalcium phosphate (M2–M25) or on control cell culture PS on day 3 after seeding. Note: Data presented as mean ± standard error of mean, from 5–9 measurements. Abbreviations: ICAM, intercellular adhesion molecule; PS, polystyrene.
Techniques Used: Cell Culture
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P-4618) and Article Title: Normal expression of DNA repair proteins, hMre11, Rad50 and Rad51 but protracted formation of Rad50 containing foci in X-irradiated skin fibroblasts from radiosensitive cancer patients Article Snippet: Paragraph title: Western blot analysis ... Equal loading and transfer were assessed by reprobing the blots with Article Title: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation Article Snippet: Paragraph title: Western blot analysis ... A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Cat#ab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or Electrotransfer:Article Title: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation Article Snippet: After the electrotransfer of the proteins, CRMP2 protein was detected using a rabbit antibody directed against CRMP2 (1:500; Abcam, Cat#ab36201, RRID:AB_731750) and the different synaptic markers were detected using mouse monoclonal antibodies directed against PSD-95 (1:20,000; Millipore, Merck, Cat#P246, RRID:AB_260911), synaptophysin (1:2000–1:20,000; Sigma, Cat#S5768, RRID:AB_477523), and syntaxin 1 A (1:2000; Sigma, Cat#S0664, RRID:AB_477483), guinea-pig polyclonal anti-vGluT1 (1:10,000; Synaptic Systems, Goettingen, Germany, Cat#135304 RRID:AB_887878), anti-vGAT (1:1000; Synaptic Systems; Cat#131004, RRID:AB_887873), and rabbit polyclonal anti-gephyrin (1:10,000; Abcam, Cat#ab25784 RRID:AB_1209349), all diluted in TBS-T (Tris-buffered saline, 0.1% Tween 20) containing 5% dried milk. .. A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Cat#ab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or TCA Precipitation:Article Title: Lysine methyltransferase G9a is not required for DNMT3A/3B anchoring to methylated nucleosomes and maintenance of DNA methylation in somatic cells Article Snippet: Western blot analysis Proteins from the same volume of each fraction (200 to 250 μl) were concentrated by TCA precipitation, dissolved in SDS/β-mercaptoethanol loading buffer, and resolved on a 4% to 15% gradient SDS/PAGE gel (Bio-Rad, Hercules, CA). .. Antibodies against H3 (ab1791), DNMT3A (ab2850), and SUV39h1 (ab12405) were purchased from Abcam Inc. (Cambridge, UK); DNMT1 (sc-20701) and DNMT3B (sc-10235) from Santa Cruz Biotech (Santa Cruz, CA); Myc epitope tag (05-724) from Upstate (now Millipore, Billerica, MA) and G9a (G 6919); Cell Culture:Article Title: LXCXE-independent chromatin remodeling by Rb/E2f mediates neuronal quiescence Article Snippet: .. 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A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Cat#ab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or other:Article Title: Cleavage of DFNA5 by caspase-3 during apoptosis mediates progression to secondary necrotic/pyroptotic cell death Article Snippet: Imaging:Article Title: Escherichia coli Subtilase Cytotoxin Induces Apoptosis Regulated by Host Bcl-2 Family Proteins Bax/Bak ▿ Article Snippet: Anti-Puma (catalog no. P-4618) and Article Title: Normal expression of DNA repair proteins, hMre11, Rad50 and Rad51 but protracted formation of Rad50 containing foci in X-irradiated skin fibroblasts from radiosensitive cancer patients Article Snippet: Equal loading and transfer were assessed by reprobing the blots with Protein Concentration:Article Title: Effects of Balance and Gait Training on the Recovery of the Motor Function in an Animal Model of Parkinson's Disease Article Snippet: The samples were centrifuged for 20 min at 15,000 rpm to obtain supernatant, and the protein concentration in the solution was quantified using Bradford method (Bio-Rad protein assay). .. To confirm if the equivalent protein, Article Title: Poly(vinyl alcohol)/gelatin Hydrogels Cultured with HepG2 Cells as a 3D Model of Hepatocellular Carcinoma: A Morphological Study Article Snippet: Sample lysates were centrifuged at 15,000 rpm for 20 min at 4 °C and protein concentration in the supernatants was determined by the bicinchoninic acid (BCA; Pierce, Rockford, IL, USA) microplate method. .. The membrane was blocked with 4% dry non-fat milk in 0.1% Tween/Tris Buffer Saline (T-TBS) and incubated overnight at 4 °C with anti Binding Assay:Article Title: Effects of Balance and Gait Training on the Recovery of the Motor Function in an Animal Model of Parkinson's Disease Article Snippet: Thereafter, the gel was transferred to a nitrocellulose membrane (Whatman GmbH, Dassel, Germany) and underwent reacted with TBX and 5% non fat dried milk for 1 hr at 4°C to prevent non-specific binding with the antibody. .. To confirm if the equivalent protein, Article Title: Beneficial Effects of Antecedent Exercise Training on Limb Motor Function and Calpain Expression in a Rat Model of Stroke Article Snippet: After electrophoresis, the proteins were transferred onto polyvinylidene fluoride membranes and nonspecific binding was blocked with 5% nonfat dry milk in tris-buffered saline and Tween 20. .. After incubation with the rabbit polyclonal anti-m-calpain antibody (1:1,000 Triple Point Biologics; Forest Grove, OR, USA) and the Nucleic Acid Electrophoresis:Article Title: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation Article Snippet: Protein extracts from cultured hippocampal neurons or hippocampal homogenates were diluted in 6× sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer ((0.5 M Tris, 0.4% SDS, pH 6.8), 30% glycerol, 10% SDS, 0.6 M dithiothreitol, and 0.012% of bromophenol blue), boiled at 95 °C for 5 min and then separated by 10% SDS-PAGE electrophoresis. .. A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Cat#ab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or Isolation:Article Title: LXCXE-independent chromatin remodeling by Rb/E2f mediates neuronal quiescence Article Snippet: .. Protein was isolated from cultured cortical neurons and western blot analyses performed as previously described, with antibodies directed toward cleaved caspase-3 (Cell Signaling, Cat No. 9664S), Rb (PharMingen, Cat No. 554136), Cyclin A2 (Abcam, Cat No. ab7965), PCNA (Millpore, Cat No. MAB424) and Protein Extraction:Article Title: Modulating BAP1 expression affects ROS homeostasis, cell motility and mitochondrial function Article Snippet: Paragraph title: Protein extraction and quantification ... After blocking with blocking buffer (LI-COR, Lincoln, NE, USA), mixed with 0.1% Tween-20, the membrane was incubated with primary antibodies overnight at 4°C (BAP1, Santa Cruz sc-4 sc-28383, 1/500; CDH2, Cell signaling #14215, 1/200; β-actin, Blocking Assay:Article Title: Modulating BAP1 expression affects ROS homeostasis, cell motility and mitochondrial function Article Snippet: .. After blocking with blocking buffer (LI-COR, Lincoln, NE, USA), mixed with 0.1% Tween-20, the membrane was incubated with primary antibodies overnight at 4°C (BAP1, Santa Cruz sc-4 sc-28383, 1/500; CDH2, Cell signaling #14215, 1/200; β-actin, Article Title: Transcriptomic analysis reveals sex-specific differences in the expression of Dcl1 and Fis1 genes in the radio-adaptive response of thymocytes to TRP53-mediated apoptosis Article Snippet: After blocking, membranes were incubated with each primary antibody in either 5 % w/v BSA or nonfat dry milk, 1× TBS, 0.1 % Tween 20 overnight at 4 °C. .. For Chloramphenicol Acetyltransferase Assay:Article Title: High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6 Article Snippet: .. Following protein transfer into 0.45 μM nitrocellulose (NC) membrane (BD, Cat.#1620115), membranes were blocked with 5% skim-milk in TBS-T buffer (0.05%) for 2 h. Then, membranes that washed and were probed using the following antibodies: Nuclear factor kappa B (NF-κB) p65 (Cell signaling Technology, Inc. Cat.#8242; 1:500 dilutions), phospho-NF-κB p65 (Ser468) (Cell signaling Technology, Inc. Cat.#3039; 1:500 dilutions) and Article Title: Oncogenic activation of the RNA binding protein NELFE and MYC signaling in hepatocellular carcinoma Article Snippet: .. Protein detection was performed using anti-NELFE (Abcam, cat# ab170104), Article Title: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation Article Snippet: .. A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Catab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or SDS Page:Article Title: Lysine methyltransferase G9a is not required for DNMT3A/3B anchoring to methylated nucleosomes and maintenance of DNA methylation in somatic cells Article Snippet: Western blot analysis Proteins from the same volume of each fraction (200 to 250 μl) were concentrated by TCA precipitation, dissolved in SDS/β-mercaptoethanol loading buffer, and resolved on a 4% to 15% gradient SDS/PAGE gel (Bio-Rad, Hercules, CA). .. Antibodies against H3 (ab1791), DNMT3A (ab2850), and SUV39h1 (ab12405) were purchased from Abcam Inc. (Cambridge, UK); DNMT1 (sc-20701) and DNMT3B (sc-10235) from Santa Cruz Biotech (Santa Cruz, CA); Myc epitope tag (05-724) from Upstate (now Millipore, Billerica, MA) and G9a (G 6919); Article Title: Modulating BAP1 expression affects ROS homeostasis, cell motility and mitochondrial function Article Snippet: 50μg of protein extracts were resolved by 4-15% SDS-PAGE (SDS-PAGE Mini- PROTEAN® TGX™ #456-1086 Biorad) and transferred to 0.2 μm nitrocellulose membranes (Biorad). .. After blocking with blocking buffer (LI-COR, Lincoln, NE, USA), mixed with 0.1% Tween-20, the membrane was incubated with primary antibodies overnight at 4°C (BAP1, Santa Cruz sc-4 sc-28383, 1/500; CDH2, Cell signaling #14215, 1/200; β-actin, Article Title: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation Article Snippet: Protein extracts from cultured hippocampal neurons or hippocampal homogenates were diluted in 6× sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer ((0.5 M Tris, 0.4% SDS, pH 6.8), 30% glycerol, 10% SDS, 0.6 M dithiothreitol, and 0.012% of bromophenol blue), boiled at 95 °C for 5 min and then separated by 10% SDS-PAGE electrophoresis. .. A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Cat#ab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or Plasmid Preparation:Article Title: A polymorphism in the lysyl oxidase propeptide domain accelerates carcinogen-induced cancer Article Snippet: .. Antibodies used were Ki-67 (cloneSP6; Thermo Scientific), LOX-PP (rabbit polyclonal IgG) used as described previously , Software:Article Title: Beneficial Effects of Antecedent Exercise Training on Limb Motor Function and Calpain Expression in a Rat Model of Stroke Article Snippet: After incubation with the rabbit polyclonal anti-m-calpain antibody (1:1,000 Triple Point Biologics; Forest Grove, OR, USA) and the Article Title: The Effects of Antecedent Exercise on Motor Function Recovery and Brain-derived Neurotrophic Factor Expression after Focal Cerebral Ischemia in Rats Article Snippet: After incubation with the anti-BDNF primary antibody (sc-546, Santa Cruz, USA), Article Title: Escherichia coli Subtilase Cytotoxin Induces Apoptosis Regulated by Host Bcl-2 Family Proteins Bax/Bak ▿ Article Snippet: Anti-Puma (catalog no. P-4618) and Article Title: Normal expression of DNA repair proteins, hMre11, Rad50 and Rad51 but protracted formation of Rad50 containing foci in X-irradiated skin fibroblasts from radiosensitive cancer patients Article Snippet: Equal loading and transfer were assessed by reprobing the blots with Article Title: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation Article Snippet: A rabbit antibody directed against GAPDH (glyceraldehyde 3-phosphate dehydrogenase) (1:1000; Abcam, Cat#ab9485 RRID:AB_307275) and mouse monoclonal antibodies against α-tubulin (1:20 000; Sigma, Cat#T6074 RRID:AB_477582) or Irradiation:Article Title: Normal expression of DNA repair proteins, hMre11, Rad50 and Rad51 but protracted formation of Rad50 containing foci in X-irradiated skin fibroblasts from radiosensitive cancer patients Article Snippet: Western blot analysis Nearly confluent nonirradiated and irradiated with 8 Gy fibroblasts were detached by trypsinisation, lyzed in RIPA buffer (10 mM tris-HCl, pH 7.4, 150 mM NaCl, 1% sodium deoxycholate, 1% Triton X-100) containing protease inhibitors (2 μ g ml−1 aprotinin, 2 μ g ml−1 leupeptin, 5 μ g ml−1 pepstatin, 1 mM phenylmethane sulphonyl fluoride) for 30 min on ice and subsequently centrifuged 10 min at 500 g . .. Equal loading and transfer were assessed by reprobing the blots with Negative Control:Article Title: Poly(vinyl alcohol)/gelatin Hydrogels Cultured with HepG2 Cells as a 3D Model of Hepatocellular Carcinoma: A Morphological Study Article Snippet: Western Blotting At the endpoint of the culture, both cell/scaffolds constructs (n = 3) and controls (scaffolds without cells as a negative control, and cells grown in monolayer up to 80% confluence—4 days—as a positive control) were washed in D-PBS and resuspended in a cell lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP-40, and antiproteases 1×, all from Sigma Aldrich). .. The membrane was blocked with 4% dry non-fat milk in 0.1% Tween/Tris Buffer Saline (T-TBS) and incubated overnight at 4 °C with anti Radio Immunoprecipitation:Article Title: Modulating BAP1 expression affects ROS homeostasis, cell motility and mitochondrial function Article Snippet: Cell pellets were lysed in radioimmunoprecipitation assay buffer (RIPA) [100mM Tris-HCl/pH7.5, 0.1M NaCl, 1mM EDTA, 1% Triton, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS)], supplemented with NaF, Na3 VO4 , phenylmethanesulfonylfluoride (PMSF) and a cocktail of protease inhibitors (cOmplete©, Roche). .. After blocking with blocking buffer (LI-COR, Lincoln, NE, USA), mixed with 0.1% Tween-20, the membrane was incubated with primary antibodies overnight at 4°C (BAP1, Santa Cruz sc-4 sc-28383, 1/500; CDH2, Cell signaling #14215, 1/200; β-actin, Lysis:Article Title: Beneficial Effects of Antecedent Exercise Training on Limb Motor Function and Calpain Expression in a Rat Model of Stroke Article Snippet: The tissue was homogenized in freshly prepared lysis buffer (1:10 w/v) and centrifuged at 12,000 × g for 30 min. .. After incubation with the rabbit polyclonal anti-m-calpain antibody (1:1,000 Triple Point Biologics; Forest Grove, OR, USA) and the Article Title: Poly(vinyl alcohol)/gelatin Hydrogels Cultured with HepG2 Cells as a 3D Model of Hepatocellular Carcinoma: A Morphological Study Article Snippet: Western Blotting At the endpoint of the culture, both cell/scaffolds constructs (n = 3) and controls (scaffolds without cells as a negative control, and cells grown in monolayer up to 80% confluence—4 days—as a positive control) were washed in D-PBS and resuspended in a cell lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP-40, and antiproteases 1×, all from Sigma Aldrich). .. The membrane was blocked with 4% dry non-fat milk in 0.1% Tween/Tris Buffer Saline (T-TBS) and incubated overnight at 4 °C with anti Article Title: The Effects of Antecedent Exercise on Motor Function Recovery and Brain-derived Neurotrophic Factor Expression after Focal Cerebral Ischemia in Rats Article Snippet: Tissue was homogenized in freshly prepared lysis buffer (1:10 w/v) and centrifuged at 12,000 ×g for 30 min. .. After incubation with the anti-BDNF primary antibody (sc-546, Santa Cruz, USA), |
