monoclonal anti beta actin  (Millipore)


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    Structured Review

    Millipore monoclonal anti beta actin
    Concentrations (percentage of PS) of <t>beta-actin</t> ( A ), vinculin ( B ), talin ( C ). osteocalcin ( D ), osteopontin ( E ), and ICAM ( F ) in MG-63 cells growing on composite material (0), composite materials with 2, 5,10, 15, and 25 vol% of nano-sized tricalcium phosphate (N2–N25) or of micro-sized tricalcium phosphate (M2–M25) or on control cell culture PS on day 3 after seeding. Note: Data presented as mean ± standard error of mean, from 5–9 measurements. Abbreviations: ICAM, intercellular adhesion molecule; PS, polystyrene.
    Monoclonal Anti Beta Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti beta actin/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti beta actin - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites"

    Article Title: Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S56661

    Concentrations (percentage of PS) of beta-actin ( A ), vinculin ( B ), talin ( C ). osteocalcin ( D ), osteopontin ( E ), and ICAM ( F ) in MG-63 cells growing on composite material (0), composite materials with 2, 5,10, 15, and 25 vol% of nano-sized tricalcium phosphate (N2–N25) or of micro-sized tricalcium phosphate (M2–M25) or on control cell culture PS on day 3 after seeding. Note: Data presented as mean ± standard error of mean, from 5–9 measurements. Abbreviations: ICAM, intercellular adhesion molecule; PS, polystyrene.
    Figure Legend Snippet: Concentrations (percentage of PS) of beta-actin ( A ), vinculin ( B ), talin ( C ). osteocalcin ( D ), osteopontin ( E ), and ICAM ( F ) in MG-63 cells growing on composite material (0), composite materials with 2, 5,10, 15, and 25 vol% of nano-sized tricalcium phosphate (N2–N25) or of micro-sized tricalcium phosphate (M2–M25) or on control cell culture PS on day 3 after seeding. Note: Data presented as mean ± standard error of mean, from 5–9 measurements. Abbreviations: ICAM, intercellular adhesion molecule; PS, polystyrene.

    Techniques Used: Cell Culture

    Related Articles

    Western Blot:

    Article Title: Disruption of forkhead transcription factor (FOXO) family members in mice reveals their functional diversification
    Article Snippet: .. Antibodies used for Western analysis were polyclonal anti-FKHR antibody (C-20, Santa Cruz Biotechnology), polyclonal anti-FKHRL1 antibody (Upstate Biotechnology, Waltham, MA), monoclonal anti-β-actin antibody (clone AC-15, Sigma), and horseradish peroxidase (HRP)-conjugated secondary antibodies (Dako). .. Immunohistochemistry of Whole-Mount Embryos and Yolk Sacs.

    Incubation:

    Article Title: Piscirickettsia salmonis Imbalances the Innate Immune Response to Succeed in a Productive Infection in a Salmonid Cell Line Model
    Article Snippet: .. The membranes were incubated with anti-IL-10, anti-hepcidin immune sera or commercial monoclonal anti-β-actin antibody (Sigma-Aldrich), diluted to 1:2000, and stored overnight at 20°C. .. The secondary anti-IgG mouse-HRP antibody (1:7000; Pierce, Thermo Scientific) was added, and the membranes were incubated at 20°C for 60 min.

    other:

    Article Title: Parkin truncating variants result in a loss-of-function phenotype
    Article Snippet: Antibodies Primary antibodies: mouse monoclonal anti-EGFP antibody (Abnova, MAB1765), mouse monoclonal anti-GFP antibody (Rockland, 600-301-215), mouse monoclonal anti-beta-actin (Sigma-Aldrich, A5441), mouse monoclonal anti-GM130 (BD Biosciences, 610822), mouse monoclonal anti-caspase 3 (Cell Signaling, 9668), mouse monoclonal anti-HDAC2 (Santa Cruz Biotechnology, sc-9959), rabbit polyclonal anti-histone H3 (Abcam, ab1791), mouse monoclonal anti-p62 (Proteintech, 66184-1-Ig), mouse monoclonal anti-TOM20 (BD Biosciences, 612278).

    Article Title: Angiotensin II affects inflammation mechanisms via AMPK-related signalling pathways in HL-1 atrial myocytes
    Article Snippet: Reagents Ang II, N-acetyl-L-cysteine (ROS scavenger), irbesartan (angiotensin receptor blocker), STO-609 (CaMKK inhibitor), compound C (AMPK inhibitor), KN-93 (CaMKII inhibitor), fluo-3 AM, 2′,7′-dichlorofluorescein diacetate (DCFDA), L-ascorbic acid, Claycomb medium, norepinephrine, gelatine, fibronectin, and monoclonal anti-β-actin antibody were purchased from Sigma Chemical Company (St. Louis, MO, USA).

    Expressing:

    Article Title: p73 is essential for vitamin D mediated osteoblastic differentiation
    Article Snippet: .. Monoclonal anti-VDR D-6 (Santa Cruz Biotechnology) and monoclonal anti-β-actin (Sigma, St. Louis, MO) antibodies were used to detect VDR and β-actin expression, respectively. .. Appropriate horseradish peroxidase-conjugated secondary antibodies (Promega, Madison, WI) were used for chemiluminescence detection with Supersignal Westpico Chemiluminescent Substrate kit (Pierce).

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    Millipore anti α sma
    Preischemic administration of stromal vascular fraction (SVF) inhibited TGF-β1-induced epithelia-mesenchymal transition (EMT) and microvascular rarefaction. (A–C): Relative abundance of E-cadherin/GAPDH (A) and <t>α-SMA/GAPDH</t> (B)
    Anti α Sma, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti α sma/product/Millipore
    Average 99 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    anti α sma - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Millipore mouse anti human β actin monoclonal antibody
    Effect of MSK1-mediated p65 Ser276 phosphorylation in IL-1β-induced SCF expression. A. Human lung fibroblasts in culture were transiently co-transfected with the pGL3e/SCF firefly luciferase construct and a Renilla luciferase construct (pRL-TK) as an internal control. Cells were pre-incubated for 1 h with a combination of SB202190 (SB; 3.5 µM) and PD98059 (PD; 20 µM) or with H89 (10 µM) and treated with IL-1β (20 U/ml). After 150 min, cells were harvested for luciferase activity measurement. The results are expressed as the level of pGL3e/SCF constructions' promoter-driven firefly luciferase expression after correcting for the transfection efficiency by pRL-TK luciferase measurements and represented as a percentage of control values. B. Fibroblasts were transfected with control and anti-MSK1 siRNA (100 nM), or transfection medium alone (control). After 48 hours, inhibition of MSK1 with siRNA was controlled by Western blotting in the cell lysate, using anti-MSK1, with <t>anti-β-actin</t> antibodies as a deposit control. Cells were treated with IL-1β (20 U/ml). SCF protein levels were assessed in the supernatant 5 hours after treatment by ELISA. C . Fibroblasts were transfected with WT or “kinase-dead” (KD) MSK1 plasmid (1 µg), WT or S276C p65 plasmids or transfection medium alone (control), and treated with IL-1β (20 U/ml). SCF protein levels were assessed by ELISA in the supernatant obtained 5 hours after treatment. Results are expressed as percentages of control values of three independent experiments performed in fibroblasts from three different donors.
    Mouse Anti Human β Actin Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human β actin monoclonal antibody/product/Millipore
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    mouse anti human β actin monoclonal antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore monoclonal rabbit anti β actin
    Nerve injury increases protein expressions of Iba1, GFAP, TNF-α, IL-1β, and MCP-1 in the spinal dorsal horn Bar graphs show the mean (+ S.E.) density of Iba1, GFAP, TNF-α, IL-1β, and MCP-1 relative to <t>β-actin</t> in the spinal dorsal horn in rats receiving either sham operation or pSNL on day 3 (A) and day 10 (B) after surgery. Samples of each molecular protein expression in each group are displayed. * p
    Monoclonal Rabbit Anti β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rabbit anti β actin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal rabbit anti β actin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Preischemic administration of stromal vascular fraction (SVF) inhibited TGF-β1-induced epithelia-mesenchymal transition (EMT) and microvascular rarefaction. (A–C): Relative abundance of E-cadherin/GAPDH (A) and α-SMA/GAPDH (B)

    Journal: Stem Cells Translational Medicine

    Article Title: Preischemic Administration of Nonexpanded Adipose Stromal Vascular Fraction Attenuates Acute Renal Ischemia/Reperfusion Injury and Fibrosis

    doi: 10.5966/sctm.2015-0223

    Figure Lengend Snippet: Preischemic administration of stromal vascular fraction (SVF) inhibited TGF-β1-induced epithelia-mesenchymal transition (EMT) and microvascular rarefaction. (A–C): Relative abundance of E-cadherin/GAPDH (A) and α-SMA/GAPDH (B)

    Article Snippet: The membranes were blocked with 10% skimmed milk for 1 hour and incubated at 4°C overnight with anti-α-SMA, anti-E-cadherin, or anti-glyceraldehyde-3-phosphate dehydrogenase (EMD Millipore), followed by incubation with horseradish peroxidase-conjugated anti-mouse IgG (Cell Signaling, Danvers, MA, ).

    Techniques:

    Effect of MSK1-mediated p65 Ser276 phosphorylation in IL-1β-induced SCF expression. A. Human lung fibroblasts in culture were transiently co-transfected with the pGL3e/SCF firefly luciferase construct and a Renilla luciferase construct (pRL-TK) as an internal control. Cells were pre-incubated for 1 h with a combination of SB202190 (SB; 3.5 µM) and PD98059 (PD; 20 µM) or with H89 (10 µM) and treated with IL-1β (20 U/ml). After 150 min, cells were harvested for luciferase activity measurement. The results are expressed as the level of pGL3e/SCF constructions' promoter-driven firefly luciferase expression after correcting for the transfection efficiency by pRL-TK luciferase measurements and represented as a percentage of control values. B. Fibroblasts were transfected with control and anti-MSK1 siRNA (100 nM), or transfection medium alone (control). After 48 hours, inhibition of MSK1 with siRNA was controlled by Western blotting in the cell lysate, using anti-MSK1, with anti-β-actin antibodies as a deposit control. Cells were treated with IL-1β (20 U/ml). SCF protein levels were assessed in the supernatant 5 hours after treatment by ELISA. C . Fibroblasts were transfected with WT or “kinase-dead” (KD) MSK1 plasmid (1 µg), WT or S276C p65 plasmids or transfection medium alone (control), and treated with IL-1β (20 U/ml). SCF protein levels were assessed by ELISA in the supernatant obtained 5 hours after treatment. Results are expressed as percentages of control values of three independent experiments performed in fibroblasts from three different donors.

    Journal: PLoS ONE

    Article Title: Ser276 Phosphorylation of NF-kB p65 by MSK1 Controls SCF Expression in Inflammation

    doi: 10.1371/journal.pone.0004393

    Figure Lengend Snippet: Effect of MSK1-mediated p65 Ser276 phosphorylation in IL-1β-induced SCF expression. A. Human lung fibroblasts in culture were transiently co-transfected with the pGL3e/SCF firefly luciferase construct and a Renilla luciferase construct (pRL-TK) as an internal control. Cells were pre-incubated for 1 h with a combination of SB202190 (SB; 3.5 µM) and PD98059 (PD; 20 µM) or with H89 (10 µM) and treated with IL-1β (20 U/ml). After 150 min, cells were harvested for luciferase activity measurement. The results are expressed as the level of pGL3e/SCF constructions' promoter-driven firefly luciferase expression after correcting for the transfection efficiency by pRL-TK luciferase measurements and represented as a percentage of control values. B. Fibroblasts were transfected with control and anti-MSK1 siRNA (100 nM), or transfection medium alone (control). After 48 hours, inhibition of MSK1 with siRNA was controlled by Western blotting in the cell lysate, using anti-MSK1, with anti-β-actin antibodies as a deposit control. Cells were treated with IL-1β (20 U/ml). SCF protein levels were assessed in the supernatant 5 hours after treatment by ELISA. C . Fibroblasts were transfected with WT or “kinase-dead” (KD) MSK1 plasmid (1 µg), WT or S276C p65 plasmids or transfection medium alone (control), and treated with IL-1β (20 U/ml). SCF protein levels were assessed by ELISA in the supernatant obtained 5 hours after treatment. Results are expressed as percentages of control values of three independent experiments performed in fibroblasts from three different donors.

    Article Snippet: Immunoblotting used the following antibodies: rabbit anti-human IκB-α polyclonal antibody (1/1000, Calbiochem, La Jolla, CA), mouse anti-human phospho- IκB-α monoclonal antibody, (1/1000, Ab-1, Oncogene Research Product, Boston, MA), rabbit anti-human phospho-Ser276 p65 antibody (1/1000, 3037, Cell Signaling Technology, Danvers MA), rabbit anti-human phospho-Ser536 p65 antibody (1/1000, 3031, Cell Signaling Technology), rabbit anti-human p65 polyclonal antibody (1/200, sc-109, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-human CBP polyclonal antibody (1/200, sc-369, Santa Cruz Biotechnology), mouse anti-human β-actin monoclonal antibody (1/5000, Ab-1, Oncogene Research Product), goat anti-human MSK1 (1/200, sc-9392, Santa Cruz Biotechnology.

    Techniques: Expressing, Transfection, Luciferase, Construct, Incubation, Activity Assay, Inhibition, Western Blot, Enzyme-linked Immunosorbent Assay, Plasmid Preparation

    Nerve injury increases protein expressions of Iba1, GFAP, TNF-α, IL-1β, and MCP-1 in the spinal dorsal horn Bar graphs show the mean (+ S.E.) density of Iba1, GFAP, TNF-α, IL-1β, and MCP-1 relative to β-actin in the spinal dorsal horn in rats receiving either sham operation or pSNL on day 3 (A) and day 10 (B) after surgery. Samples of each molecular protein expression in each group are displayed. * p

    Journal: Neuroscience

    Article Title: EZH2 regulates spinal neuroinflammation in rats with neuropathic pain

    doi: 10.1016/j.neuroscience.2017.02.041

    Figure Lengend Snippet: Nerve injury increases protein expressions of Iba1, GFAP, TNF-α, IL-1β, and MCP-1 in the spinal dorsal horn Bar graphs show the mean (+ S.E.) density of Iba1, GFAP, TNF-α, IL-1β, and MCP-1 relative to β-actin in the spinal dorsal horn in rats receiving either sham operation or pSNL on day 3 (A) and day 10 (B) after surgery. Samples of each molecular protein expression in each group are displayed. * p

    Article Snippet: The membranes were blocked with 5% milk or 5% BSA in TBST, and then incubated respectively overnight at 4 °C with polyclonal rabbit anti-GFAP (1:1,000, cell signaling), polyclonal rabbit anti-Iba1 (1:1,000; Wako), rabbit anti-TNF-α (1:500; Millipore), rabbit anti-IL-1β (1:500; Millipore), rabbit anti-MCP-1 (1:500; Abcam), rabbit anti-EZH2 (1:500; Abcam), rabbit anti-H3K27TM (1:500; Epigentek), rabbit anti-total histone H3 (1;1,000; cell signaling) primary antibodies, or a monoclonal rabbit anti-β-actin (1:2,000; Millipore) primary antibody as a loading control.

    Techniques: Expressing

    Pre-emptive DZNep or GSK126 treatment significantly attenuates the increased levels of EZH2 and H3K27TM in the spinal dorsal horn induced by nerve injury (A): Data were obtained from the spinal dorsal horn of animals treated with daily intrathecal injection of either 20 nM DZNep (in 10 μL) or vehicle (10 μL) for 9 days. (B): Data were obtained from the spinal dorsal horn of animals treated with daily intrathecal injection of either 5 nM GSK126 (in 10 μL) or vehicle (10 μL) for 9 days. Bar graphs show the mean (+ S.E.) relative density ratio of EZH2 over β-actin and H3K27TM over total-histone H3. Western blot samples of each molecular protein in each group are displayed. * p

    Journal: Neuroscience

    Article Title: EZH2 regulates spinal neuroinflammation in rats with neuropathic pain

    doi: 10.1016/j.neuroscience.2017.02.041

    Figure Lengend Snippet: Pre-emptive DZNep or GSK126 treatment significantly attenuates the increased levels of EZH2 and H3K27TM in the spinal dorsal horn induced by nerve injury (A): Data were obtained from the spinal dorsal horn of animals treated with daily intrathecal injection of either 20 nM DZNep (in 10 μL) or vehicle (10 μL) for 9 days. (B): Data were obtained from the spinal dorsal horn of animals treated with daily intrathecal injection of either 5 nM GSK126 (in 10 μL) or vehicle (10 μL) for 9 days. Bar graphs show the mean (+ S.E.) relative density ratio of EZH2 over β-actin and H3K27TM over total-histone H3. Western blot samples of each molecular protein in each group are displayed. * p

    Article Snippet: The membranes were blocked with 5% milk or 5% BSA in TBST, and then incubated respectively overnight at 4 °C with polyclonal rabbit anti-GFAP (1:1,000, cell signaling), polyclonal rabbit anti-Iba1 (1:1,000; Wako), rabbit anti-TNF-α (1:500; Millipore), rabbit anti-IL-1β (1:500; Millipore), rabbit anti-MCP-1 (1:500; Abcam), rabbit anti-EZH2 (1:500; Abcam), rabbit anti-H3K27TM (1:500; Epigentek), rabbit anti-total histone H3 (1;1,000; cell signaling) primary antibodies, or a monoclonal rabbit anti-β-actin (1:2,000; Millipore) primary antibody as a loading control.

    Techniques: Injection, Western Blot

    Nerve injury increases EZH2 protein expression and H3K27TM levels in spinal dorsal horn Bar graphs show the mean (+S.E.) of relative density ratio of EZH2 over β-actin and H3K27TM over total-histone H3 in the spinal dorsal horn of rats receiving either sham operation or pSNL on day 3 (A) and day 10 (B) after surgery. Western blot samples of each molecular protein in each group are displayed. * p

    Journal: Neuroscience

    Article Title: EZH2 regulates spinal neuroinflammation in rats with neuropathic pain

    doi: 10.1016/j.neuroscience.2017.02.041

    Figure Lengend Snippet: Nerve injury increases EZH2 protein expression and H3K27TM levels in spinal dorsal horn Bar graphs show the mean (+S.E.) of relative density ratio of EZH2 over β-actin and H3K27TM over total-histone H3 in the spinal dorsal horn of rats receiving either sham operation or pSNL on day 3 (A) and day 10 (B) after surgery. Western blot samples of each molecular protein in each group are displayed. * p

    Article Snippet: The membranes were blocked with 5% milk or 5% BSA in TBST, and then incubated respectively overnight at 4 °C with polyclonal rabbit anti-GFAP (1:1,000, cell signaling), polyclonal rabbit anti-Iba1 (1:1,000; Wako), rabbit anti-TNF-α (1:500; Millipore), rabbit anti-IL-1β (1:500; Millipore), rabbit anti-MCP-1 (1:500; Abcam), rabbit anti-EZH2 (1:500; Abcam), rabbit anti-H3K27TM (1:500; Epigentek), rabbit anti-total histone H3 (1;1,000; cell signaling) primary antibodies, or a monoclonal rabbit anti-β-actin (1:2,000; Millipore) primary antibody as a loading control.

    Techniques: Expressing, Western Blot