african green monkey kidney epithelial vero e6 cells  (ATCC)


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    ATCC african green monkey kidney epithelial vero e6 cells
    African Green Monkey Kidney Epithelial Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney epithelial vero cells  (ATCC)


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    ATCC african green monkey kidney epithelial vero cells
    African Green Monkey Kidney Epithelial Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney epithelial vero cells  (ATCC)


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    ATCC african green monkey kidney epithelial vero cells
    African Green Monkey Kidney Epithelial Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney epithelial cells vero  (ATCC)


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    ATCC african green monkey kidney epithelial cells vero
    African Green Monkey Kidney Epithelial Cells Vero, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney epithelial cells vero e6  (ATCC)


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    ATCC african green monkey kidney epithelial cells vero e6
    African Green Monkey Kidney Epithelial Cells Vero E6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    green monkey kidney epithelial vero e6 cells  (ATCC)


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    ATCC green monkey kidney epithelial vero e6 cells
    Green Monkey Kidney Epithelial Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney epithelial cells vero 81  (ATCC)


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    ATCC african green monkey kidney epithelial cells vero 81
    a The viral load of pangolin-CoV-HKU4-P251T in series passages in <t>Vero</t> <t>81</t> cell cultures. The viral load are expressed as copies per microliter (µL). b Negative stain electron microscopy verified the extracellular virus particle of pangolin-CoV-HKU4-P251T. Original magnification ×80 K. c Fluorescence in situ hybridization locating ORF1ab gene of pangolin-CoV-HKU4-P251T at 48 HPI. Nuclei, DAPI (blue); ORF1ab probe, Quasar 570 (red). Representative microscopy fields are shown. Original magnification ×200. Each imaging experiment was performed at least three times independently with similar results, and the representative microscopy fields are shown ( b and c ). d Genetic variation analysis of pangolin-CoV-HKU4-P251T during in vitro passage. The parental pangolin-CoV-HKU4-P251T strain (GenBank accession No. OM009282) was used as the reference sequence. Source data are provided as a Source Data file.
    African Green Monkey Kidney Epithelial Cells Vero 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice"

    Article Title: Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice

    Journal: Nature Communications

    doi: 10.1038/s41467-024-45453-2

    a The viral load of pangolin-CoV-HKU4-P251T in series passages in Vero 81 cell cultures. The viral load are expressed as copies per microliter (µL). b Negative stain electron microscopy verified the extracellular virus particle of pangolin-CoV-HKU4-P251T. Original magnification ×80 K. c Fluorescence in situ hybridization locating ORF1ab gene of pangolin-CoV-HKU4-P251T at 48 HPI. Nuclei, DAPI (blue); ORF1ab probe, Quasar 570 (red). Representative microscopy fields are shown. Original magnification ×200. Each imaging experiment was performed at least three times independently with similar results, and the representative microscopy fields are shown ( b and c ). d Genetic variation analysis of pangolin-CoV-HKU4-P251T during in vitro passage. The parental pangolin-CoV-HKU4-P251T strain (GenBank accession No. OM009282) was used as the reference sequence. Source data are provided as a Source Data file.
    Figure Legend Snippet: a The viral load of pangolin-CoV-HKU4-P251T in series passages in Vero 81 cell cultures. The viral load are expressed as copies per microliter (µL). b Negative stain electron microscopy verified the extracellular virus particle of pangolin-CoV-HKU4-P251T. Original magnification ×80 K. c Fluorescence in situ hybridization locating ORF1ab gene of pangolin-CoV-HKU4-P251T at 48 HPI. Nuclei, DAPI (blue); ORF1ab probe, Quasar 570 (red). Representative microscopy fields are shown. Original magnification ×200. Each imaging experiment was performed at least three times independently with similar results, and the representative microscopy fields are shown ( b and c ). d Genetic variation analysis of pangolin-CoV-HKU4-P251T during in vitro passage. The parental pangolin-CoV-HKU4-P251T strain (GenBank accession No. OM009282) was used as the reference sequence. Source data are provided as a Source Data file.

    Techniques Used: Staining, Electron Microscopy, Virus, Fluorescence, In Situ Hybridization, Microscopy, Imaging, In Vitro, Sequencing

    green monkey kidney epithelial vero e6 cells  (ATCC)


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    ATCC green monkey kidney epithelial vero e6 cells
    Green Monkey Kidney Epithelial Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    african green monkey kidney epithelial vertebrate derived vero cells  (ATCC)


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    ATCC african green monkey kidney epithelial vertebrate derived vero cells
    Cell association of R. parkeri wild-type and R. parkeri ompB STOP ::tn . ( A ) <t>Tick-derived</t> <t>ISE6</t> cells. ( B ) <t>Vero</t> cells. Rickettsiae were inoculated onto cells (multiplicity of infection, 5). After cells and supernatant were collected at different times, rickettsiae were quantified by qPCR. Each bar represents six wells for two biological replicates (three wells/replicate). Statistical analysis with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.
    African Green Monkey Kidney Epithelial Vertebrate Derived Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host"

    Article Title: Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host

    Journal: Infection and Immunity

    doi: 10.1128/iai.00515-23

    Cell association of R. parkeri wild-type and R. parkeri ompB STOP ::tn . ( A ) Tick-derived ISE6 cells. ( B ) Vero cells. Rickettsiae were inoculated onto cells (multiplicity of infection, 5). After cells and supernatant were collected at different times, rickettsiae were quantified by qPCR. Each bar represents six wells for two biological replicates (three wells/replicate). Statistical analysis with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.
    Figure Legend Snippet: Cell association of R. parkeri wild-type and R. parkeri ompB STOP ::tn . ( A ) Tick-derived ISE6 cells. ( B ) Vero cells. Rickettsiae were inoculated onto cells (multiplicity of infection, 5). After cells and supernatant were collected at different times, rickettsiae were quantified by qPCR. Each bar represents six wells for two biological replicates (three wells/replicate). Statistical analysis with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.

    Techniques Used: Derivative Assay, Infection, Comparison

    Internalization of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Immunofluorescence of Vero cells infected with rickettsiae (multiplicity of infection, 5). Left panel: extracellular rickettsiae only are labeled with Alexa 594 (red). Middle panel: after permeabilization, all rickettsiae are labeled with Alexa 488 (green). Right panel: the intracellular rickettsiae were determined by signal subtraction (total rickettsiae minus extracellular rickettsiae). Dashed oval, representative intracellular rickettsiae. Yellow arrow, representative extracellular rickettsiae. Scale bar, 50 µm. ( B ) Internalization of rickettsiae in Vero cells after infection, reported as percentage of intracellular to total rickettsiae. Points represent 20 fields for 2 independent replicates (10 fields/replicate). Comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.
    Figure Legend Snippet: Internalization of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Immunofluorescence of Vero cells infected with rickettsiae (multiplicity of infection, 5). Left panel: extracellular rickettsiae only are labeled with Alexa 594 (red). Middle panel: after permeabilization, all rickettsiae are labeled with Alexa 488 (green). Right panel: the intracellular rickettsiae were determined by signal subtraction (total rickettsiae minus extracellular rickettsiae). Dashed oval, representative intracellular rickettsiae. Yellow arrow, representative extracellular rickettsiae. Scale bar, 50 µm. ( B ) Internalization of rickettsiae in Vero cells after infection, reported as percentage of intracellular to total rickettsiae. Points represent 20 fields for 2 independent replicates (10 fields/replicate). Comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.

    Techniques Used: Immunofluorescence, Infection, Labeling, Comparison

    Growth kinetics and immunofluorescence of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Infected Vero cells were processed for qPCR. Statistical comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA. * P < 0.05. ( B ) Microscopy images of R. parkeri wild-type-infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( C ) Microscopy images of R. parkeri ompB STOP ::tn -infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( D ) The intensity of Alexa 488 was measured using ImageJ from three fields per replicate. Statistical comparisons of two independent replicates between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. *** P < 0.001.
    Figure Legend Snippet: Growth kinetics and immunofluorescence of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Infected Vero cells were processed for qPCR. Statistical comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA. * P < 0.05. ( B ) Microscopy images of R. parkeri wild-type-infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( C ) Microscopy images of R. parkeri ompB STOP ::tn -infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( D ) The intensity of Alexa 488 was measured using ImageJ from three fields per replicate. Statistical comparisons of two independent replicates between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. *** P < 0.001.

    Techniques Used: Immunofluorescence, Infection, Microscopy, Comparison

    Primers and probes used for quantitative polymerase chain reaction to detect Rickettsia species and host cells <xref ref-type= a " title="Primers and probes used for quantitative polymerase chain reaction to detect Rickettsia species ... " property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Primers and probes used for quantitative polymerase chain reaction to detect Rickettsia species and host cells a

    Techniques Used: Real-time Polymerase Chain Reaction, Sequencing, Amplification

    african green monkey kidney epithelial vero cells  (ATCC)


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    ATCC african green monkey kidney epithelial vero cells
    African Green Monkey Kidney Epithelial Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC african green monkey kidney epithelial vero e6 cells
    African Green Monkey Kidney Epithelial Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC african green monkey kidney epithelial vero cells
    African Green Monkey Kidney Epithelial Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC african green monkey kidney epithelial cells vero
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    ATCC african green monkey kidney epithelial cells vero e6
    African Green Monkey Kidney Epithelial Cells Vero E6, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC green monkey kidney epithelial vero e6 cells
    Green Monkey Kidney Epithelial Vero E6 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC african green monkey kidney epithelial cells vero 81
    a The viral load of pangolin-CoV-HKU4-P251T in series passages in <t>Vero</t> <t>81</t> cell cultures. The viral load are expressed as copies per microliter (µL). b Negative stain electron microscopy verified the extracellular virus particle of pangolin-CoV-HKU4-P251T. Original magnification ×80 K. c Fluorescence in situ hybridization locating ORF1ab gene of pangolin-CoV-HKU4-P251T at 48 HPI. Nuclei, DAPI (blue); ORF1ab probe, Quasar 570 (red). Representative microscopy fields are shown. Original magnification ×200. Each imaging experiment was performed at least three times independently with similar results, and the representative microscopy fields are shown ( b and c ). d Genetic variation analysis of pangolin-CoV-HKU4-P251T during in vitro passage. The parental pangolin-CoV-HKU4-P251T strain (GenBank accession No. OM009282) was used as the reference sequence. Source data are provided as a Source Data file.
    African Green Monkey Kidney Epithelial Cells Vero 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC african green monkey kidney epithelial vertebrate derived vero cells
    Cell association of R. parkeri wild-type and R. parkeri ompB STOP ::tn . ( A ) <t>Tick-derived</t> <t>ISE6</t> cells. ( B ) <t>Vero</t> cells. Rickettsiae were inoculated onto cells (multiplicity of infection, 5). After cells and supernatant were collected at different times, rickettsiae were quantified by qPCR. Each bar represents six wells for two biological replicates (three wells/replicate). Statistical analysis with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.
    African Green Monkey Kidney Epithelial Vertebrate Derived Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/african green monkey kidney epithelial vertebrate derived vero cells/product/ATCC
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    a The viral load of pangolin-CoV-HKU4-P251T in series passages in Vero 81 cell cultures. The viral load are expressed as copies per microliter (µL). b Negative stain electron microscopy verified the extracellular virus particle of pangolin-CoV-HKU4-P251T. Original magnification ×80 K. c Fluorescence in situ hybridization locating ORF1ab gene of pangolin-CoV-HKU4-P251T at 48 HPI. Nuclei, DAPI (blue); ORF1ab probe, Quasar 570 (red). Representative microscopy fields are shown. Original magnification ×200. Each imaging experiment was performed at least three times independently with similar results, and the representative microscopy fields are shown ( b and c ). d Genetic variation analysis of pangolin-CoV-HKU4-P251T during in vitro passage. The parental pangolin-CoV-HKU4-P251T strain (GenBank accession No. OM009282) was used as the reference sequence. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice

    doi: 10.1038/s41467-024-45453-2

    Figure Lengend Snippet: a The viral load of pangolin-CoV-HKU4-P251T in series passages in Vero 81 cell cultures. The viral load are expressed as copies per microliter (µL). b Negative stain electron microscopy verified the extracellular virus particle of pangolin-CoV-HKU4-P251T. Original magnification ×80 K. c Fluorescence in situ hybridization locating ORF1ab gene of pangolin-CoV-HKU4-P251T at 48 HPI. Nuclei, DAPI (blue); ORF1ab probe, Quasar 570 (red). Representative microscopy fields are shown. Original magnification ×200. Each imaging experiment was performed at least three times independently with similar results, and the representative microscopy fields are shown ( b and c ). d Genetic variation analysis of pangolin-CoV-HKU4-P251T during in vitro passage. The parental pangolin-CoV-HKU4-P251T strain (GenBank accession No. OM009282) was used as the reference sequence. Source data are provided as a Source Data file.

    Article Snippet: The original small intestine samples from Malayan pangolins, from which the whole genome sequence of pangolin-CoV-HKU4-P251T had been obtained (accession number OM009282) , were homogenized and propagated on African green monkey kidney epithelial cells Vero 81 (ATCC CCL-81).

    Techniques: Staining, Electron Microscopy, Virus, Fluorescence, In Situ Hybridization, Microscopy, Imaging, In Vitro, Sequencing

    Cell association of R. parkeri wild-type and R. parkeri ompB STOP ::tn . ( A ) Tick-derived ISE6 cells. ( B ) Vero cells. Rickettsiae were inoculated onto cells (multiplicity of infection, 5). After cells and supernatant were collected at different times, rickettsiae were quantified by qPCR. Each bar represents six wells for two biological replicates (three wells/replicate). Statistical analysis with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.

    Journal: Infection and Immunity

    Article Title: Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host

    doi: 10.1128/iai.00515-23

    Figure Lengend Snippet: Cell association of R. parkeri wild-type and R. parkeri ompB STOP ::tn . ( A ) Tick-derived ISE6 cells. ( B ) Vero cells. Rickettsiae were inoculated onto cells (multiplicity of infection, 5). After cells and supernatant were collected at different times, rickettsiae were quantified by qPCR. Each bar represents six wells for two biological replicates (three wells/replicate). Statistical analysis with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.

    Article Snippet: Ixodes scapularis embryonic-derived ISE6 cells (RRID: CVCL_Z170, ATCC) and African green monkey kidney epithelial (vertebrate-derived) Vero cells (CCL-81, RRID: CVCL_0059, ATCC) were maintained as described ( , ).

    Techniques: Derivative Assay, Infection, Comparison

    Internalization of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Immunofluorescence of Vero cells infected with rickettsiae (multiplicity of infection, 5). Left panel: extracellular rickettsiae only are labeled with Alexa 594 (red). Middle panel: after permeabilization, all rickettsiae are labeled with Alexa 488 (green). Right panel: the intracellular rickettsiae were determined by signal subtraction (total rickettsiae minus extracellular rickettsiae). Dashed oval, representative intracellular rickettsiae. Yellow arrow, representative extracellular rickettsiae. Scale bar, 50 µm. ( B ) Internalization of rickettsiae in Vero cells after infection, reported as percentage of intracellular to total rickettsiae. Points represent 20 fields for 2 independent replicates (10 fields/replicate). Comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.

    Journal: Infection and Immunity

    Article Title: Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host

    doi: 10.1128/iai.00515-23

    Figure Lengend Snippet: Internalization of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Immunofluorescence of Vero cells infected with rickettsiae (multiplicity of infection, 5). Left panel: extracellular rickettsiae only are labeled with Alexa 594 (red). Middle panel: after permeabilization, all rickettsiae are labeled with Alexa 488 (green). Right panel: the intracellular rickettsiae were determined by signal subtraction (total rickettsiae minus extracellular rickettsiae). Dashed oval, representative intracellular rickettsiae. Yellow arrow, representative extracellular rickettsiae. Scale bar, 50 µm. ( B ) Internalization of rickettsiae in Vero cells after infection, reported as percentage of intracellular to total rickettsiae. Points represent 20 fields for 2 independent replicates (10 fields/replicate). Comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. * P < 0.05; ** P < 0.01; **** P < 0.0001.

    Article Snippet: Ixodes scapularis embryonic-derived ISE6 cells (RRID: CVCL_Z170, ATCC) and African green monkey kidney epithelial (vertebrate-derived) Vero cells (CCL-81, RRID: CVCL_0059, ATCC) were maintained as described ( , ).

    Techniques: Immunofluorescence, Infection, Labeling, Comparison

    Growth kinetics and immunofluorescence of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Infected Vero cells were processed for qPCR. Statistical comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA. * P < 0.05. ( B ) Microscopy images of R. parkeri wild-type-infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( C ) Microscopy images of R. parkeri ompB STOP ::tn -infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( D ) The intensity of Alexa 488 was measured using ImageJ from three fields per replicate. Statistical comparisons of two independent replicates between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. *** P < 0.001.

    Journal: Infection and Immunity

    Article Title: Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host

    doi: 10.1128/iai.00515-23

    Figure Lengend Snippet: Growth kinetics and immunofluorescence of R. parkeri wild-type and R. parkeri ompB STOP ::tn in Vero cells. ( A ) Infected Vero cells were processed for qPCR. Statistical comparisons between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA. * P < 0.05. ( B ) Microscopy images of R. parkeri wild-type-infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( C ) Microscopy images of R. parkeri ompB STOP ::tn -infected Vero cells at 0, 24, 48, 72, and 96 hpi. Data represent six wells for two independent replicates (three wells/replicate). Scale bar = 50 µm. ( D ) The intensity of Alexa 488 was measured using ImageJ from three fields per replicate. Statistical comparisons of two independent replicates between R. parkeri wild-type and R. parkeri ompB STOP ::tn with two-way ANOVA and Šidák multiple comparison test. *** P < 0.001.

    Article Snippet: Ixodes scapularis embryonic-derived ISE6 cells (RRID: CVCL_Z170, ATCC) and African green monkey kidney epithelial (vertebrate-derived) Vero cells (CCL-81, RRID: CVCL_0059, ATCC) were maintained as described ( , ).

    Techniques: Immunofluorescence, Infection, Microscopy, Comparison

    Primers and probes used for quantitative polymerase chain reaction to detect Rickettsia species and host cells <xref ref-type= a " width="100%" height="100%">

    Journal: Infection and Immunity

    Article Title: Critical roles of Rickettsia parkeri outer membrane protein B (OmpB) in the tick host

    doi: 10.1128/iai.00515-23

    Figure Lengend Snippet: Primers and probes used for quantitative polymerase chain reaction to detect Rickettsia species and host cells a

    Article Snippet: Ixodes scapularis embryonic-derived ISE6 cells (RRID: CVCL_Z170, ATCC) and African green monkey kidney epithelial (vertebrate-derived) Vero cells (CCL-81, RRID: CVCL_0059, ATCC) were maintained as described ( , ).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing, Amplification