Monensin, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 92 stars, based on 1 article reviews
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1) Product Images from "S1P lyase regulates DNA damage responses through a novel sphingolipid feedback mechanism"
Article Title: S1P lyase regulates DNA damage responses through a novel sphingolipid feedback mechanism
Journal: Cell Death & Disease
Figure Legend Snippet: ASMase is responsible for the effects of SPL on the DDR. ( a ) Control and SPL hi cells were left untreated or pretreated with Monensin (50 nM) for 3 h then cells were exposed to IR and caspase-3 activity was measured 24 h after radiation exposure. ( b ) SPL hi cells were either mock transfected or transfected with siRNA against human ASMase gene ( SMPD1 ). After 48 h, cells were irradiated and harvested after 24 h. ASMase activity in knockdown and mock transfected cells was assayed. Caspase-3 activity was measured using DEVD-pNA as a substrate. Data represent mean±S.D. of three independent experiments. ( * P
Techniques Used: Activity Assay, Transfection, Irradiation
2) Product Images from "Image-based drug screen identifies HDAC inhibitors as novel Golgi disruptors synergizing with JQ1"
Article Title: Image-based drug screen identifies HDAC inhibitors as novel Golgi disruptors synergizing with JQ1
Journal: Molecular Biology of the Cell
Figure Legend Snippet: Connectivity map analysis of classical Golgi-fragmenting drugs predicts HDAC inhibitors and DNA-damaging agents as compounds with a similar mode of action. The gene expression signatures of A549 cells treated for 20 h with 71 nM BFA, 5 µM GCA, or 10 µM monensin were determined and used to query the LINCS L1000 database to identify compounds with similar gene expression profiles. (A) Heat map representation of all the gene expression signatures in the L1000 database and their connectivity scores in comparison with our own BFA, GCA, and monensin input expression profiles. The data were sorted based on the similarity score for BFA. (B) Detailed view of the top 50 correlations identified in this analysis and sorted by their similarity to the BFA profile. Blue boxes indicate DNA-damaging agents; red boxes indicate HDAC inhibitors. (C) Pie chart summarizing the compounds in the top 50 hit list.
Techniques Used: Expressing
3) Product Images from "Aberrant T Helper 17 Cells and Related Cytokines in Bone Marrow Microenvironment of Patients with Acute Myeloid Leukemia"
Article Title: Aberrant T Helper 17 Cells and Related Cytokines in Bone Marrow Microenvironment of Patients with Acute Myeloid Leukemia
Journal: Clinical and Developmental Immunology
Figure Legend Snippet: Th subsets and their related cytokines in ND, relapsed-refractory, and CR AML patients and controls. (a) The percentage of BM Th17 cells was significantly decreased in ND AML patients compared with CR patients or controls after stimulation with phorbol myristate acetate, ionomycin, and monensin for 4 h. (b) The level of BM plasma IL-17A showed the decreased trend in the ND, relapsed-refractory, or CR AML patients compared with controls, though no statistical significance exists. (c) The percentage of BM Th1 cells was significantly decreased in ND AML patients compared with relapsed-refractory or CR patients or controls. (d) The level of BM plasma IFN- γ was decreased in the ND AML patients compared with CR patients.
Article Title: Preferential Recruitment of Th17 Cells to Cervical Cancer via CCR6-CCL20 Pathway
Article Snippet: Cells were stimulated to secrete cytokines for 4h with 100 ng/mL phorbol myristate acetate (PMA), 1 μg/mL ionomycin (Sigma—Aldrich) and 2 μM
Article Title: Thymic Stromal Lymphopoietin Attenuates the Development of Atherosclerosis in ApoE−/− Mice
Article Snippet: Cultures were stimulated with phorbol myristate acetate (20 ng/mL) plus ionomycin (1 μg/mL) from Alexis Biochemicals for 4 hours in the presence of 2 μmol/mL
Article Title: The Treg/Th17 Imbalance in Patients with Obstructive Sleep Apnoea Syndrome
Article Snippet: Cultures were stimulated with phorbol myristate acetate (PMA, 50 ng/mL) plus ionomycin (1 μ M) for 4 h in the presence of
Article Title: Peripheral Th17/Treg imbalance in patients with atherosclerotic cerebral infarction
Article Snippet: Cultures were stimulated with phorbol myristate acetate (PMA, 25 ng/ml) plus ionomycin (1 μg/ml) for 4 h, in the presence of
Article Title: Intranasal immunization with heat shock protein 60 induces CD4+CD25+GARP+ and type 1 regulatory T cells and inhibits early atherosclerosis
Article Snippet: For the analysis of CD4+ IFN‐γ+ (Th1), CD4+ IL‐4+ (Th2) and CD4+ IL‐17+ (Th17) T cells, the cells were stimulated with phorbol myristate acetate (PMA, 20 ng/ml) plus
Article Title: Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients
Article Snippet: Flow cytometric analysis of Th1, Th17 and Th22 cells Peripheral blood was collected from all subjects and cultured under stimulation conditions. .. Heparinized peripheral whole blood (400 µl) in an equal volume of RPMI-1640 medium (Tiangen Biotech Co., Ltd., Beijing, China) were incubated for 4 h at 37°C with 5% CO2 in the presence of 25 ng/ml phorbol myristate acetate, 1 µg/ml ionomycin and 1.7 µg/ml
Article Title: Aberrant Circulating Th17 Cells in Patients with B-Cell Non-Hodgkin’s Lymphoma
Article Snippet: Flow Cytometric Analysis Flow cytometry was used to study membrane makers and intracellular cytokines to identify the cytokine-producing cells. .. Briefly, heparinized peripheral blood (400 μl) with an equal volume of RPMI 1640 medium (Hyclone, USA) was incubated for 4 h at 37°C, 5% CO2 in the presence of 25 ng/ml of phorbol myristate acetate (PMA), 1 μg/ml of ionomycin, and 1.7 μg/ml
Article Title: The existence of Th22, pure Th17 and Th1 cells in CIN and Cervical Cancer along with their frequency variation in different stages of cervical cancer
Article Snippet: Flow Cytometric Analysis Intracellular cytokines were evaluated by flow cytometry to identify the cytokine-producing cells. .. Briefly, heparinized peripheral whole blood (200 μl) with an equal volume of Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma Chemical, St Louis, MO, USA) was incubated for 4 h at 37 °C and 5 % CO2 in the presence of 25 ng/ml of phorbolmyristate acetate (PMA), 1 μg/ml of ionomycin and 1.7 μg/ml of