monensin solution (Thermo Fisher)
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Name:
eBioscience Monensin Solution 1000X
Description:
Monensin is an inhibitor of intracellular protein transport Incubation of cells in culture with Monensin leads to blockade of protein transport to the Golgi complex GC and accumulation of proteins in the endoplasmic reticulum ER Addition of Monensin during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines Monensin is effective for enhanced detection of a majority of mouse and human intracellular cytokines however it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Brefeldin A in their specific assay system Reported ApplicationIntracellular Staining Followed by Flow Cytometric Analysis
Catalog Number:
00-4505-51
Price:
None
Category:
Lab Reagents and Chemicals
Applications:
Cell Analysis|Cellular Imaging|Flow Cytometry
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Structured Review
Thermo Fisher
monensin solution
Monensin is an inhibitor of intracellular protein transport Incubation of cells in culture with Monensin leads to blockade of protein transport to the Golgi complex GC and accumulation of proteins in the endoplasmic reticulum ER Addition of Monensin during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines Monensin is effective for enhanced detection of a majority of mouse and human intracellular cytokines however it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Brefeldin A in their specific assay system Reported ApplicationIntracellular Staining Followed by Flow Cytometric Analysis
https://www.bioz.com/result/monensin solution/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Monensin is an inhibitor of intracellular protein transport Incubation of cells in culture with Monensin leads to blockade of protein transport to the Golgi complex GC and accumulation of proteins in the endoplasmic reticulum ER Addition of Monensin during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines Monensin is effective for enhanced detection of a majority of mouse and human intracellular cytokines however it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Brefeldin A in their specific assay system Reported ApplicationIntracellular Staining Followed by Flow Cytometric Analysis
https://www.bioz.com/result/monensin solution/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monensin solution - by Bioz Stars,
2021-03
96/100 stars
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For intracellular staining for IL-13 (ebio13A coupled to PE) and IFN-γ (XMG1.2 coupled to allophycocyanin), T cells were activated for 5 h in a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A, and |
![Cytokine profile of B cell-derived polyclonal B2 versus immune splenocyte-derived polyclonal CD4 T cell lines, shown by the presence of CD4γ13 T cells in the genital tract and spleen. (A) Polyclonal B cell-derived line 2 (B2) and splenocyte-derived line 2 (spl2) were activated for 5 h with PMA-ionomycin-brefeldin A-monensin and then stained for CD8 (negative stain for CD4 T cells) versus IL-13 and IFN-γ versus IL-13. (B) Specificity and cytokine profiles for B2 and five splenocyte-derived T cell lines (spl1 to -5). 2.5 × 10 4 T cells were coincubated with 5 × 10 4 purified immune B cells unpulsed (APCs) or pulsed with uvMoPn (antigen [Ag]) in triplicate. Cell culture supernatants harvested at 48 h, and levels of IFN-γ, IL-13, and IL-4 were determined by ELISA. Shown are comparisons between control (Con) and antigen-pulsed (Ag) B cell activation for each cytokine and T cell line. *, P](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778355/bin/zii9990922850002.jpg)
