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Becton Dickinson monensin golgistop
Monensin Golgistop, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monensin golgistop/product/Becton Dickinson
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monensin golgistop - by Bioz Stars, 2021-03
86/100 stars

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Recombinase Polymerase Amplification:

Article Title: Cholangiocarcinoma presents a distinct myeloid-derived suppressor cell signature compared to other hepatobiliary cancers
Article Snippet: .. ReagentsFluorophore-conjugated anti-human CD3 (UCHT1), CD4 (SK3), CD8 (RPA-T8), CD14 (MφP9), CD15 (HI98), CD25 (M-A251), CD33 (WM53), CD107a (H4A3), CD127 (HIL-7R-M21) and HLA-DR (G46-6) antibodies were purchased from BD Biosciences. .. Anti-mouse Ly6G (1A8), Ly6C (HK1.4), CD11b (M1/70) and CD3 (145-2C11) were purchased from Biolegend.

Expressing:

Article Title: Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function
Article Snippet: The gating or sorting strategies for all flow cytometry analysis in this study are included in Supplementary Fig. . .. For CD107a expression analysis , splenic cells were mixed with Yac-1 cells at a 2:1 E:T ratio in a V-bottom 96-well plate in the presence of anti-CD107a antibody (BD Biosciences, 1D4B) and BD GolgiStop™ protein transport inhibitor (BD Biosciences). ..

Clone Assay:

Article Title: Human ?-Cell Killing by Autoreactive Preproinsulin-Specific CD8 T Cells Is Predominantly Granule-Mediated With the Potency Dependent Upon T-Cell Receptor Avidity
Article Snippet: Assessing intracellular protein and CD107a surface expression. .. Anti-CD107a (fluorescein isothiocyanate; clone H4A3; BD Biosciences) was added to 5 × 105 cloned CD8 T cells in FACS tubes containing X-Vivo 15/5% AB serum/IL-7 (10 ng/mL), IL-15 (0.1 ng/mL), and 2.5% Cellkine (ZeptoMetrix Corporation). .. Islet or nonislet target cell preparations were added to tubes (5 × 105 ) for 1 h at 37°C (5% CO2 ) before 0.7 μg/mL monensin (GolgiStop; BD Biosciences) and 1 μg/mL brefeldin A (GolgiPlug; BD Biosciences) were added to cocultures.

FACS:

Article Title: Human ?-Cell Killing by Autoreactive Preproinsulin-Specific CD8 T Cells Is Predominantly Granule-Mediated With the Potency Dependent Upon T-Cell Receptor Avidity
Article Snippet: Assessing intracellular protein and CD107a surface expression. .. Anti-CD107a (fluorescein isothiocyanate; clone H4A3; BD Biosciences) was added to 5 × 105 cloned CD8 T cells in FACS tubes containing X-Vivo 15/5% AB serum/IL-7 (10 ng/mL), IL-15 (0.1 ng/mL), and 2.5% Cellkine (ZeptoMetrix Corporation). .. Islet or nonislet target cell preparations were added to tubes (5 × 105 ) for 1 h at 37°C (5% CO2 ) before 0.7 μg/mL monensin (GolgiStop; BD Biosciences) and 1 μg/mL brefeldin A (GolgiPlug; BD Biosciences) were added to cocultures.

Staining:

Article Title: Distinct Roles of Vaccinia Virus NF-κB Inhibitor Proteins A52, B15, and K7 in the Immune Response
Article Snippet: .. For intracellular cytokine staining (ICS), splenocytes were resuspended in RPMI 1640 with 10% FCS and 1 μg/ml Golgiplug (BD), monensin (eBioscience), and anti-CD107a (1D4B; BD), restimulated with peptides (6 h, 37°C, 5% CO2 ), stained for surface markers with anti-CD3 (145-2C11), anti-CD4 (GK1.5), and anti-CD8 (53-6.7) (all from BD), fixed, permeabilized (Cytofix/Cytoperm kit; BD), and stained intracellularly with anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG 1.2), and anti-TNF-α (MP6-X722) (all from BD). .. Peritoneal exudate cells (PECs) were stained with anti-Ly6G (1A8), anti-CD3 (145-2C11), anti-CD11b (M1/70), anti-CD19 (1D3), anti-MHC class II (1-A/1-E; 2G9), anti-CD4 (GK1.5), and anti-CD8 (53-6.7) (all from BD), anti-CD45 (30-F11; Biolegend), anti-CD11c (N418) and anti-F4/80 (BM8; both from eBioscience), and anti-NKp46 (29A1.4; BioLegend).

Article Title: DNA Prime-Adenovirus Boost Immunization Induces a Vigorous and Multifunctional T-Cell Response against Hepadnaviral Proteins in the Mouse and Woodchuck Model
Article Snippet: Cell surface staining was performed using anti-CD8 (clone 56.6-7; BD Pharmingen) and anti-CD4 (clone L3T4; BD Pharmingen) T-cell antibodies. .. Staining of a CD107a molecule (monoclonal anti-mouse CD107a FITC-conjugated antibody, clone GB12, at a dilution of 1:200 [BD Pharmingen]) was performed during a 5-h restimulation of the splenocytes. .. Dead cells were excluded from analyses using 7-aminoactinomycin D (7AAD) (Becton Dickinson, Heidelberg, Germany).

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    Becton Dickinson monensin
    NK cells from Rae-1 Tg and WT mice produce comparable amounts of IFN-γ and CD107a when stimulated with Ly49H agonists. A and B , Freshly isolated splenocytes from Rae-1 Tg or WT littermate controls were enriched for NK cells and stimulated with plate-bound Abs against the NKRs NKG2D, Ly49D, and Ly49H or an isotype-matched control Ig in the presence of <t>monensin</t> (BD GolgiStop). After 4 h, cells were stained for intracellular IFN-γ and surface CD107a expression. Plots show the percentage of NK1.1 + TCRβ − cells that produce ( A ) IFN-γ and ( B ) CD107a. C and D , NK cells enriched as in A were incubated with Ba/F3 or Ba/F3-m157 targets in the presence of monensin. After 4 h, cells were stained for intracellular IFN-γ and surface CD107a expression. Plots show the percentage of NK1.1 + TCRβ − cells that produce ( C ) IFN-γ and ( D ) CD107a. Data are representative of three experiments.
    Monensin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    86/100 stars
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    NK cells from Rae-1 Tg and WT mice produce comparable amounts of IFN-γ and CD107a when stimulated with Ly49H agonists. A and B , Freshly isolated splenocytes from Rae-1 Tg or WT littermate controls were enriched for NK cells and stimulated with plate-bound Abs against the NKRs NKG2D, Ly49D, and Ly49H or an isotype-matched control Ig in the presence of monensin (BD GolgiStop). After 4 h, cells were stained for intracellular IFN-γ and surface CD107a expression. Plots show the percentage of NK1.1 + TCRβ − cells that produce ( A ) IFN-γ and ( B ) CD107a. C and D , NK cells enriched as in A were incubated with Ba/F3 or Ba/F3-m157 targets in the presence of monensin. After 4 h, cells were stained for intracellular IFN-γ and surface CD107a expression. Plots show the percentage of NK1.1 + TCRβ − cells that produce ( C ) IFN-γ and ( D ) CD107a. Data are representative of three experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Intact NKG2D-Independent Function of NK Cells Chronically Stimulated with the NKG2D Ligand Rae-1

    doi: 10.4049/jimmunol.1000397

    Figure Lengend Snippet: NK cells from Rae-1 Tg and WT mice produce comparable amounts of IFN-γ and CD107a when stimulated with Ly49H agonists. A and B , Freshly isolated splenocytes from Rae-1 Tg or WT littermate controls were enriched for NK cells and stimulated with plate-bound Abs against the NKRs NKG2D, Ly49D, and Ly49H or an isotype-matched control Ig in the presence of monensin (BD GolgiStop). After 4 h, cells were stained for intracellular IFN-γ and surface CD107a expression. Plots show the percentage of NK1.1 + TCRβ − cells that produce ( A ) IFN-γ and ( B ) CD107a. C and D , NK cells enriched as in A were incubated with Ba/F3 or Ba/F3-m157 targets in the presence of monensin. After 4 h, cells were stained for intracellular IFN-γ and surface CD107a expression. Plots show the percentage of NK1.1 + TCRβ − cells that produce ( C ) IFN-γ and ( D ) CD107a. Data are representative of three experiments.

    Article Snippet: After 4 h at 37°C in the presence of monensin (BD GolgiStop), cells were washed and stained for intracellular IFN-γ by using an Intracellular Staining kit ( ).

    Techniques: Mouse Assay, Isolation, Staining, Expressing, Incubation

    Cytokine production by T FH cell subsets. Purified CD4 + T cells from healthy controls were cultured for 5h with PMA, ionomycin and monensin. CD4 + T cells were then stained with fluorescently-labeled Abs specific for CD4, CD45RA, CXCR5, CCR6 and CXCR3. (A) T FH subset distribution was determined thanks to CXCR3 and CCR6 expression on gated CD4 + CD45RA - CXCR5 + T cells allowing the identification of T FH 17 cells (CXCR3 - CCR6 + , light grey), T FH 2 cells (CXCR3 - CCR6 - , dark grey) and T FH 1 cells (CXCR3 + CCR6 - , thick black). Frequencies of IL-4, IFN-γ, IL-17 and IL-21 positive cells were determined by intracellular staining on each T FH subset after setting the threshold using isotype control staining. Dot plots (B) from one healthy control is shown as example and histograms (C) from 3 healthy controls and 3 SLE patients are shown. Data are expressed as % ± sem.

    Journal: PLoS ONE

    Article Title: Circulating TFH Subset Distribution Is Strongly Affected in Lupus Patients with an Active Disease

    doi: 10.1371/journal.pone.0075319

    Figure Lengend Snippet: Cytokine production by T FH cell subsets. Purified CD4 + T cells from healthy controls were cultured for 5h with PMA, ionomycin and monensin. CD4 + T cells were then stained with fluorescently-labeled Abs specific for CD4, CD45RA, CXCR5, CCR6 and CXCR3. (A) T FH subset distribution was determined thanks to CXCR3 and CCR6 expression on gated CD4 + CD45RA - CXCR5 + T cells allowing the identification of T FH 17 cells (CXCR3 - CCR6 + , light grey), T FH 2 cells (CXCR3 - CCR6 - , dark grey) and T FH 1 cells (CXCR3 + CCR6 - , thick black). Frequencies of IL-4, IFN-γ, IL-17 and IL-21 positive cells were determined by intracellular staining on each T FH subset after setting the threshold using isotype control staining. Dot plots (B) from one healthy control is shown as example and histograms (C) from 3 healthy controls and 3 SLE patients are shown. Data are expressed as % ± sem.

    Article Snippet: Enriched CD4+ T cells (purity > 95%) were then stimulated 5h with 25ng/ml phorbol myristate acetate (PMA) and 1µM ionomycin (Sigma-Aldrich) in the presence of monensin (BD GolgiStop™, BD Biosciences, San Diego, CA, USA).

    Techniques: Purification, Cell Culture, Staining, Labeling, Expressing

    Analysis of IFN-γ–producing T cells from mice infected with T. gondii and treated with sIL-15Rα. C57BL/6 mice immunized and subsequently challenged with 76K strain were treated with sIL-15Rα (T1) or the control protein (M4) as described in Fig. 1 . (A) Spleen cells were harvested on day 7 and (B) 14 after challenge infection, pooled ( n = 3), and cultured in vitro with PMA, ionomycin, and monensin for 4 h. The cultured cells were then labeled for CD4 or CD8 before intracellular staining for IFN-γ. Data are presented as number (mean ± SD) of CD4 + or CD8 + T cells positive for IFN-γ and are pooled from two different experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: Treatment with Soluble Interleukin-15R? Exacerbates Intracellular Parasitic Infection by Blocking the Development of Memory CD8+ T Cell Response

    doi: 10.1084/jem.20011915

    Figure Lengend Snippet: Analysis of IFN-γ–producing T cells from mice infected with T. gondii and treated with sIL-15Rα. C57BL/6 mice immunized and subsequently challenged with 76K strain were treated with sIL-15Rα (T1) or the control protein (M4) as described in Fig. 1 . (A) Spleen cells were harvested on day 7 and (B) 14 after challenge infection, pooled ( n = 3), and cultured in vitro with PMA, ionomycin, and monensin for 4 h. The cultured cells were then labeled for CD4 or CD8 before intracellular staining for IFN-γ. Data are presented as number (mean ± SD) of CD4 + or CD8 + T cells positive for IFN-γ and are pooled from two different experiments.

    Article Snippet: The cells (106 cells/well) were cultured in 96-well plates and stimulated with PMA (10 ng/ml; Sigma-Aldrich), ionomycin (500 ng/ml; Sigma-Aldrich), and monensin (2 μM, GolgiStop; BD PharMingen).

    Techniques: Mouse Assay, Infection, Cell Culture, In Vitro, Labeling, Staining

    Ligands for CXCR3 and CCR5 are overexpressed in the livers of hepatosplenic subjects. The highest proportions of Tregs among PBMCs were associated with the lowest levels of IFNγ production by PBMCs. A-B) Expression of the ligands for CXCR3 , CCR5 and CCR7 in the liver and spleen of eight hepatosplenic patients (Group 2). Messenger RNA levels are expressed relative to the arithmetic mean values obtained for 11 healthy controls. C ) The proportion of IFNγ + cells among blood CD4 + T cells is negatively correlated ( r = -0.73, p = 0.002) with the proportion of eTregs in the blood in Group 1. The proportion of IFNγ + CD4 + T cells was determined after 6h of stimulation with PMA, ionomycin and monensin. Nonparametric statistical tests were used * p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: FOXP3+ Regulatory T Cells in Hepatic Fibrosis and Splenomegaly Caused by Schistosoma japonicum: The Spleen May Be a Major Source of Tregs in Subjects with Splenomegaly

    doi: 10.1371/journal.pntd.0004306

    Figure Lengend Snippet: Ligands for CXCR3 and CCR5 are overexpressed in the livers of hepatosplenic subjects. The highest proportions of Tregs among PBMCs were associated with the lowest levels of IFNγ production by PBMCs. A-B) Expression of the ligands for CXCR3 , CCR5 and CCR7 in the liver and spleen of eight hepatosplenic patients (Group 2). Messenger RNA levels are expressed relative to the arithmetic mean values obtained for 11 healthy controls. C ) The proportion of IFNγ + cells among blood CD4 + T cells is negatively correlated ( r = -0.73, p = 0.002) with the proportion of eTregs in the blood in Group 1. The proportion of IFNγ + CD4 + T cells was determined after 6h of stimulation with PMA, ionomycin and monensin. Nonparametric statistical tests were used * p

    Article Snippet: They were incubated with 100 ng/ml PMA, 1 μg/ml ionomycin and monensin (BD GolgiStop) for 6 hours at 37°C before intracellular cytokine labeling.

    Techniques: Expressing