monarch total rna miniprep kits  (New England Biolabs)


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    Name:
    Monarch Total RNA Miniprep Enzyme Pack
    Description:
    Monarch Total RNA Miniprep Enzyme Pack
    Catalog Number:
    T2019L
    Price:
    63
    Category:
    RNA Purification Kit Components
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    Structured Review

    New England Biolabs monarch total rna miniprep kits
    Monarch Total RNA Miniprep Enzyme Pack
    Monarch Total RNA Miniprep Enzyme Pack
    https://www.bioz.com/result/monarch total rna miniprep kits/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch total rna miniprep kits - by Bioz Stars, 2021-05
    86/100 stars

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    Quantitative RT-PCR:

    Article Title: DNA mismatch repair controls the host innate response and cell fate after influenza virus infection
    Article Snippet: For experiments using siRNA-treated cells, infection was done at 48 h after siRNA transfection to ensure maximum knockdown of target genes. .. For cell culture-based qRT-PCR experiments, total RNA was collected using Monarch Total RNA Miniprep Kits (NEB). .. For the mouse lung IAV NP qRT-PCR, lungs were collected at 4 dpi (with 1200 PFU virus) and homogenized directly in Trizol, before using chloroform and isopropyl alcohol to complete the RNA extraction.

    Article Title: ETV7 represses a subset of interferon-stimulated genes that restrict influenza viruses
    Article Snippet: Membranes were washed five times in PBS with 0.1% Tween-20 and then an anti-rabbit-HRP secondary antibody (Thermo, A16104) was added for 1 h. The membrane was then washed five times and Clarity or Clarity Max ECL substrate (Bio-Rad) was added before being exposed to film and developed. .. RT-qPCR Total RNA was collected using Monarch Total RNA Miniprep Kits (NEB). .. One-step RT-qPCR was performed with commercial TaqMan assays from Thermo for ETV7 (Hs00903229_m1), C1GALT1 (Hs00863329_g1), NUP153 (Hs01018919_m1), ISG15 (Hs00196051_m1), MX1 (Hs00895608_m1), IFIT1 (Hs00356631_g1), RSAD2 (Hs00895608_m1), IFI44L (Hs00915292_m1), IFIT3 (Hs01922752_s1), IFIT5 (Hs00202721_m1), and IRF9 (Hs00196051_m1) using the EXPRESS One-Step Superscript qRT-PCR Kit on an Applied Biosystems StepOnePlus instrument.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Tailoring the Oxidative Stress Tolerance of Clostridium tyrobutyricum CCTCC W428 by Introducing Trehalose Biosynthetic Capability.
    Article Snippet: Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. .. Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. ..

    Expressing:

    Article Title: Tailoring the Oxidative Stress Tolerance of Clostridium tyrobutyricum CCTCC W428 by Introducing Trehalose Biosynthetic Capability.
    Article Snippet: Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. .. Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. ..

    Purification:

    Article Title: Tailoring the Oxidative Stress Tolerance of Clostridium tyrobutyricum CCTCC W428 by Introducing Trehalose Biosynthetic Capability.
    Article Snippet: Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. .. Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. ..

    Recombinant:

    Article Title: Tailoring the Oxidative Stress Tolerance of Clostridium tyrobutyricum CCTCC W428 by Introducing Trehalose Biosynthetic Capability.
    Article Snippet: Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. .. Fermentations employing anaerobes always suffer from the restriction of stringent anaerobic conditions during the production of bulk and fine chemicals. ..

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    New England Biolabs total rna extraction total rna
    Abundance of histone transcripts compared to the remainder of de novo-assembled transcripts in Nanochlorum <t>eucaryotum</t> (SAG 55.87) and five other eukaryotes, including three algae (C. reinhardtii, D. tertiolecta, C. zofingiensis). Abundance of the most highly expressed histone transcript (solid lines) and the summed abundance of all histone transcripts (dashed lines) is indicated for each species. See Methods for details on the underlying <t>RNA-Seq</t> data, assembly and quantification process.
    Total Rna Extraction Total Rna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total rna extraction total rna/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total rna extraction total rna - by Bioz Stars, 2021-05
    99/100 stars
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    Abundance of histone transcripts compared to the remainder of de novo-assembled transcripts in Nanochlorum eucaryotum (SAG 55.87) and five other eukaryotes, including three algae (C. reinhardtii, D. tertiolecta, C. zofingiensis). Abundance of the most highly expressed histone transcript (solid lines) and the summed abundance of all histone transcripts (dashed lines) is indicated for each species. See Methods for details on the underlying RNA-Seq data, assembly and quantification process.

    Journal: bioRxiv

    Article Title: Slaying the last unicorn - discovery of histones in the microalga Nanochlorum eucaryotum

    doi: 10.1101/2020.10.22.350256

    Figure Lengend Snippet: Abundance of histone transcripts compared to the remainder of de novo-assembled transcripts in Nanochlorum eucaryotum (SAG 55.87) and five other eukaryotes, including three algae (C. reinhardtii, D. tertiolecta, C. zofingiensis). Abundance of the most highly expressed histone transcript (solid lines) and the summed abundance of all histone transcripts (dashed lines) is indicated for each species. See Methods for details on the underlying RNA-Seq data, assembly and quantification process.

    Article Snippet: Total RNA extraction Total RNA was isolated from a 31-day-old N. eucaryotum (SAG 55.87) culture consisting of ~1.9 × 107 cells in total.

    Techniques: RNA Sequencing Assay