monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    monarch rna cleanup kit - by Bioz Stars, 2023-03
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    monarch rna cleanup kit - by Bioz Stars, 2023-03
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    monarch rna cleanup kit - by Bioz Stars, 2023-03
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    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Shown are Northern blots <t>of</t> <t>avcI</t> <t>RNA</t> using a biotinylated probe complementary to avcI (top) and Western blots of AvcD-6xHis using anti-6xHis antibody (bottom) during rifampicin treatment (250 μg/mL) ( A ), spectinomycin treatment (200 μg/mL) ( B ), T5 infection ( C ), and T7 infection ( D ) at a MOI of 5.
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup kit - by Bioz Stars, 2023-03
    86/100 stars

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    1) Product Images from "Time to lysis determines phage sensitivity to a cytidine deaminase toxin/antitoxin bacterial defense system"

    Article Title: Time to lysis determines phage sensitivity to a cytidine deaminase toxin/antitoxin bacterial defense system

    Journal: bioRxiv

    doi: 10.1101/2023.02.09.527960

    Shown are Northern blots of avcI RNA using a biotinylated probe complementary to avcI (top) and Western blots of AvcD-6xHis using anti-6xHis antibody (bottom) during rifampicin treatment (250 μg/mL) ( A ), spectinomycin treatment (200 μg/mL) ( B ), T5 infection ( C ), and T7 infection ( D ) at a MOI of 5.
    Figure Legend Snippet: Shown are Northern blots of avcI RNA using a biotinylated probe complementary to avcI (top) and Western blots of AvcD-6xHis using anti-6xHis antibody (bottom) during rifampicin treatment (250 μg/mL) ( A ), spectinomycin treatment (200 μg/mL) ( B ), T5 infection ( C ), and T7 infection ( D ) at a MOI of 5.

    Techniques Used: Northern Blot, Western Blot, Infection

    monarch rna cleanup kit  (New England Biolabs)


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    New England Biolabs monarch rna cleanup kit
    Overview of the URA5 knockout strategies. As a proof of principle for CRISPR/Cas-mediated genetic modifications, we attempted to knockout the orotate phosphoribosyltransferase gene ( URA5 ) to counter select 5-fluoroorotic acid (5FOA). We selected three sgRNAs from the library, which showed no off-target activities within the C. oleaginosus genome through in-silico prediction, and selectively targeted URA5 . We then followed three parallel strategies to implement the Cas9 platform in C. oleaginosus . Both spheroplast batches prepared by Glucanex and HEST were used to test all strategies. a Strategy one and two: genome editing by Cas nuclease delivered into spheroplasts by electroporation in two forms separately: protein (Cas:sgRNA ribonucleoprotein [RNP]) <t>and</t> <t>mRNA.</t> In both strategies one single guide <t>RNA</t> (sgRNA) was used to target URA5 . A single stranded DNA (ssDNA) was simultaneously transferred to introduce the repair sequences, including base deletions and base substitutions. b Strategy three: genome editing using Cas nickase as an RNP. Here, two sgRNAs targeting the leading and lagging strands were delivered to create the double-strand break (DSB). The repair ssDNA included base insertions and substitutions. A non-cutting restriction site (HindIII) was also introduced in the URA5 loci of mutants. The protospacer adjacent motifs (PAMs) were mutated in all strategies to prevent further DNA cleavage after the repair. c – e Colonies on selection agar plates with URA5 knockout using Cas mRNA, nuclease protein, and nickase protein, respectively. f The agarose gel electrophoresis of digested URA5 gene from WT and Δura5 strains. The URA5 locus was PCR amplified from the genomic DNA of mutants and WT and subjected to fast digestion by HindIII restriction enzyme. The digestion resulted in appearance of two smaller bands in the gene isolated from the Δura5 strain, indicating the integration of repair DNAs by Cas nickase. The WT URA 5 gene was not digested
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup kit/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup kit - by Bioz Stars, 2023-03
    86/100 stars

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    1) Product Images from "Mastering targeted genome engineering of GC-rich oleaginous yeast for tailored plant oil alternatives for the food and chemical sector"

    Article Title: Mastering targeted genome engineering of GC-rich oleaginous yeast for tailored plant oil alternatives for the food and chemical sector

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-023-02033-1

    Overview of the URA5 knockout strategies. As a proof of principle for CRISPR/Cas-mediated genetic modifications, we attempted to knockout the orotate phosphoribosyltransferase gene ( URA5 ) to counter select 5-fluoroorotic acid (5FOA). We selected three sgRNAs from the library, which showed no off-target activities within the C. oleaginosus genome through in-silico prediction, and selectively targeted URA5 . We then followed three parallel strategies to implement the Cas9 platform in C. oleaginosus . Both spheroplast batches prepared by Glucanex and HEST were used to test all strategies. a Strategy one and two: genome editing by Cas nuclease delivered into spheroplasts by electroporation in two forms separately: protein (Cas:sgRNA ribonucleoprotein [RNP]) and mRNA. In both strategies one single guide RNA (sgRNA) was used to target URA5 . A single stranded DNA (ssDNA) was simultaneously transferred to introduce the repair sequences, including base deletions and base substitutions. b Strategy three: genome editing using Cas nickase as an RNP. Here, two sgRNAs targeting the leading and lagging strands were delivered to create the double-strand break (DSB). The repair ssDNA included base insertions and substitutions. A non-cutting restriction site (HindIII) was also introduced in the URA5 loci of mutants. The protospacer adjacent motifs (PAMs) were mutated in all strategies to prevent further DNA cleavage after the repair. c – e Colonies on selection agar plates with URA5 knockout using Cas mRNA, nuclease protein, and nickase protein, respectively. f The agarose gel electrophoresis of digested URA5 gene from WT and Δura5 strains. The URA5 locus was PCR amplified from the genomic DNA of mutants and WT and subjected to fast digestion by HindIII restriction enzyme. The digestion resulted in appearance of two smaller bands in the gene isolated from the Δura5 strain, indicating the integration of repair DNAs by Cas nickase. The WT URA 5 gene was not digested
    Figure Legend Snippet: Overview of the URA5 knockout strategies. As a proof of principle for CRISPR/Cas-mediated genetic modifications, we attempted to knockout the orotate phosphoribosyltransferase gene ( URA5 ) to counter select 5-fluoroorotic acid (5FOA). We selected three sgRNAs from the library, which showed no off-target activities within the C. oleaginosus genome through in-silico prediction, and selectively targeted URA5 . We then followed three parallel strategies to implement the Cas9 platform in C. oleaginosus . Both spheroplast batches prepared by Glucanex and HEST were used to test all strategies. a Strategy one and two: genome editing by Cas nuclease delivered into spheroplasts by electroporation in two forms separately: protein (Cas:sgRNA ribonucleoprotein [RNP]) and mRNA. In both strategies one single guide RNA (sgRNA) was used to target URA5 . A single stranded DNA (ssDNA) was simultaneously transferred to introduce the repair sequences, including base deletions and base substitutions. b Strategy three: genome editing using Cas nickase as an RNP. Here, two sgRNAs targeting the leading and lagging strands were delivered to create the double-strand break (DSB). The repair ssDNA included base insertions and substitutions. A non-cutting restriction site (HindIII) was also introduced in the URA5 loci of mutants. The protospacer adjacent motifs (PAMs) were mutated in all strategies to prevent further DNA cleavage after the repair. c – e Colonies on selection agar plates with URA5 knockout using Cas mRNA, nuclease protein, and nickase protein, respectively. f The agarose gel electrophoresis of digested URA5 gene from WT and Δura5 strains. The URA5 locus was PCR amplified from the genomic DNA of mutants and WT and subjected to fast digestion by HindIII restriction enzyme. The digestion resulted in appearance of two smaller bands in the gene isolated from the Δura5 strain, indicating the integration of repair DNAs by Cas nickase. The WT URA 5 gene was not digested

    Techniques Used: Knock-Out, CRISPR, In Silico, Electroporation, Introduce, Selection, Agarose Gel Electrophoresis, Amplification, Isolation

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    New England Biolabs monarch rna cleanup kit
    Monarch Rna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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