moloney murine leukemia virus rt  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukemia virus rt
    ( A ) Northern blot analysis of sig1 and sig2 transcript levels in total RNA (10 μg/lane) isolated from different tissues of etiolated and illuminated maize seedlings. RNA samples were from leaves (LL) and roots (LR) of illuminated seedlings (20 hr under light), leaves (DL), and roots (DR) of etiolated seedlings, and MCs and BSCs of illuminated seedlings (20 hr under light). Essentially equal loading of RNA was shown by ethidium bromide staining of RNA gels. ( B ) Further assessment of sig1 and sig2 transcripts in total RNAs from A by RT-PCR. RNA was reverse-transcribed by using <t>Moloney</t> murine leukemia virus RT with sig1 and sig2 gene-specific primers, and PCR was performed to amplify the cDNA. RT-PCR also was performed for the 18S rRNA gene as a positive control. The definitions of the six different tissues are the same as in A .
    Moloney Murine Leukemia Virus Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus rt/product/Thermo Fisher
    Average 92 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus rt - by Bioz Stars, 2020-07
    92/100 stars

    Images

    1) Product Images from "Characterization of two chloroplast RNA polymerase sigma factors from Zea mays: Photoregulation and differential expression"

    Article Title: Characterization of two chloroplast RNA polymerase sigma factors from Zea mays: Photoregulation and differential expression

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    ( A ) Northern blot analysis of sig1 and sig2 transcript levels in total RNA (10 μg/lane) isolated from different tissues of etiolated and illuminated maize seedlings. RNA samples were from leaves (LL) and roots (LR) of illuminated seedlings (20 hr under light), leaves (DL), and roots (DR) of etiolated seedlings, and MCs and BSCs of illuminated seedlings (20 hr under light). Essentially equal loading of RNA was shown by ethidium bromide staining of RNA gels. ( B ) Further assessment of sig1 and sig2 transcripts in total RNAs from A by RT-PCR. RNA was reverse-transcribed by using Moloney murine leukemia virus RT with sig1 and sig2 gene-specific primers, and PCR was performed to amplify the cDNA. RT-PCR also was performed for the 18S rRNA gene as a positive control. The definitions of the six different tissues are the same as in A .
    Figure Legend Snippet: ( A ) Northern blot analysis of sig1 and sig2 transcript levels in total RNA (10 μg/lane) isolated from different tissues of etiolated and illuminated maize seedlings. RNA samples were from leaves (LL) and roots (LR) of illuminated seedlings (20 hr under light), leaves (DL), and roots (DR) of etiolated seedlings, and MCs and BSCs of illuminated seedlings (20 hr under light). Essentially equal loading of RNA was shown by ethidium bromide staining of RNA gels. ( B ) Further assessment of sig1 and sig2 transcripts in total RNAs from A by RT-PCR. RNA was reverse-transcribed by using Moloney murine leukemia virus RT with sig1 and sig2 gene-specific primers, and PCR was performed to amplify the cDNA. RT-PCR also was performed for the 18S rRNA gene as a positive control. The definitions of the six different tissues are the same as in A .

    Techniques Used: Northern Blot, Isolation, Staining, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Positive Control

    Related Articles

    Synthesized:

    Article Title: Perturbation of Defense Pathways by Low-Dose Arsenic Exposure in Zebrafish Embryos
    Article Snippet: .. First-strand cDNA was synthesized from 2 mg of total RNA using Moloney murine leukemia virus RT from a RETROscript kit (Ambion, Austin, TX) according to the manufacturer’s protocol. ..

    Isolation:

    Article Title: A transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata)
    Article Snippet: .. Quantitative real-time RT-PCR One microgram of total RNA isolated from liver or white skeletal muscle of S. aurata was reverse-transcribed to cDNA using Moloney murine leukemia virus RT (Life Technologies, Carlsbad, CA, USA) for 1 h at 37 °C in the presence of random hexamer primers. .. The cDNA product was used for subsequent quantitative real-time PCR (qPCR).

    Quantitative RT-PCR:

    Article Title: A transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata)
    Article Snippet: .. Quantitative real-time RT-PCR One microgram of total RNA isolated from liver or white skeletal muscle of S. aurata was reverse-transcribed to cDNA using Moloney murine leukemia virus RT (Life Technologies, Carlsbad, CA, USA) for 1 h at 37 °C in the presence of random hexamer primers. .. The cDNA product was used for subsequent quantitative real-time PCR (qPCR).

    Article Title: Post-transcriptional Regulation of Programmed Cell Death 4 (PDCD4) mRNA by the RNA-binding Proteins Human Antigen R (HuR) and T-cell Intracellular Antigen 1 (TIA1) *
    Article Snippet: .. Reverse transcriptase reactions with Moloney murine leukemia virus RT (Invitrogen) used 1 μg of RNA for a final concentration of 50 ng/μl cDNA per sample that was used for quantitative RT-PCR. .. Isolation and enrichment of miRNAs from MCF-7 cells was performed using an miRNeasy mini kit (Qiagen).

    Article Title: The Polyadenosine RNA-binding Protein, Zinc Finger Cys3His Protein 14 (ZC3H14), Regulates the Pre-mRNA Processing of a Key ATP Synthase Subunit mRNA *
    Article Snippet: .. Reverse transcriptase reactions were carried out with Random Primers (hexadeoxynucleotides; Promega) and Moloney murine leukemia virus RT (Invitrogen) using 1 μg of RNA for an estimated concentration of ∼50 ng/μl cDNA per sample that was used for quantitative RT-PCR. .. For qRT-PCR analyses, 1 μg of total RNA was transcribed to cDNA as described above.

    Real-time Polymerase Chain Reaction:

    Article Title: Dynamic Partnership between TFIIH, PGC-1α and SIRT1 Is Impaired in Trichothiodystrophy
    Article Snippet: .. Retrotranscription and real-time quantitative PCR Total RNAs (2 µg) were reverse-transcribed with Moloney murine leukemia virus RT (Invitrogen) using random hexanucleotides. .. Real-time quantitative PCR was done using the “FastStart DNA Master SYBR Green” kit and the Lightcycler apparatus (Roche Diagnostic).

    Concentration Assay:

    Article Title: Post-transcriptional Regulation of Programmed Cell Death 4 (PDCD4) mRNA by the RNA-binding Proteins Human Antigen R (HuR) and T-cell Intracellular Antigen 1 (TIA1) *
    Article Snippet: .. Reverse transcriptase reactions with Moloney murine leukemia virus RT (Invitrogen) used 1 μg of RNA for a final concentration of 50 ng/μl cDNA per sample that was used for quantitative RT-PCR. .. Isolation and enrichment of miRNAs from MCF-7 cells was performed using an miRNeasy mini kit (Qiagen).

    Article Title: The Polyadenosine RNA-binding Protein, Zinc Finger Cys3His Protein 14 (ZC3H14), Regulates the Pre-mRNA Processing of a Key ATP Synthase Subunit mRNA *
    Article Snippet: .. Reverse transcriptase reactions were carried out with Random Primers (hexadeoxynucleotides; Promega) and Moloney murine leukemia virus RT (Invitrogen) using 1 μg of RNA for an estimated concentration of ∼50 ng/μl cDNA per sample that was used for quantitative RT-PCR. .. For qRT-PCR analyses, 1 μg of total RNA was transcribed to cDNA as described above.

    Random Hexamer Labeling:

    Article Title: A transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata)
    Article Snippet: .. Quantitative real-time RT-PCR One microgram of total RNA isolated from liver or white skeletal muscle of S. aurata was reverse-transcribed to cDNA using Moloney murine leukemia virus RT (Life Technologies, Carlsbad, CA, USA) for 1 h at 37 °C in the presence of random hexamer primers. .. The cDNA product was used for subsequent quantitative real-time PCR (qPCR).

    Article Title: IFNγ Enhances Microglial Reactions to Hippocampal Axonal Degeneration
    Article Snippet: .. Briefly, 3 μg mRNA was added to a tube containing 400 U Moloney murine leukemia virus-RT (Life Technologies), 10 m m of each dNTP (Pharmacia Biotech, Montreal, Quebec, Canada), 50 pmol random hexamer primer (Boehringer Mannheim, Laval, QC, Canada), 23 U RNA guard RNase inhibitor (Pharmacia Biotech), and a 5× first-strand buffer containing 250 m m Tris-HCl, pH 8.3, 375 m m KCl, and 15 m m MgCl2 (Life Technologies). ..

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    https://www.bioz.com/result/moloney murine leukemia virus rt/product/Thermo Fisher
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