moloney murine leukemia virus reverse transcriptase  (TaKaRa)

 
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    Name:
    MMLV Reverse Transcriptase
    Description:
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    Catalog Number:
    639575
    Price:
    None
    Size:
    8 000 Units
    Category:
    MMLV RT GPR Reverse transcriptases cDNA synthesis
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    Structured Review

    TaKaRa moloney murine leukemia virus reverse transcriptase
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

    Amplification:

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

    Synthesized:

    Article Title: AIMP3 Deletion Induces Acute Radiation Syndrome-like Phenotype in Mice
    Article Snippet: .. For siRNA-mediated knock-down validation, cells were lyzed in Trizol (Gibco) and cDNA were synthesized from the 1 μg of total RNA using MMLV-RT (Clontech). .. Level of AIMP3, BRCA1, XRCC5 and GARS were measured by SyGr (Bio-rad) using AB7500 real time PCR.

    Article Title: Characterization and analysis of the transcriptome in Gymnocypris selincuoensis on the Qinghai-Tibetan Plateau using single-molecule long-read sequencing and RNA-seq
    Article Snippet: .. First-stand cDNA was synthesized using M-MLV Reverse Transcriptase (TaKaRa, Japan) with oligo (dT) primer following the manufacturer’s protocol. .. PCR products were checked using 2.0% agarose gel stained with ethidium bromide.

    Article Title: Role of Proteases in the Release of Porcine Epidemic Diarrhea Virus from Infected Cells ▿
    Article Snippet: .. First-strand cDNA was synthesized by using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Takara Bio Inc., Shiga, Japan) and oligo(dT)16 (Applied Biosystems, Foster City, CA). .. To determine the copy number of viral RNA, real-time PCR was performed in duplicate with synthesized cDNA by using the LightCycler 480 system and Probe Master reagents (Roche, Basel, Switzerland) with a specific primer and probe set (sense, 5′-ACGGCGACTACTCAGC-3′; probe, 6-carboxyfluorescein [FAM]-5′-CCGCAAACGGGTGC-3′-minor groove binder [MGB]; antisense, GGGCATAAAGGGATAAT) constructed to amplify the nucleocapsid gene of PEDV.

    Sequencing:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: ZIP4, a Novel Determinant of Tumor Invasion in Hepatocellular Carcinoma, Contributes to Tumor Recurrence after Liver Transplantation
    Article Snippet: .. Real-time polymerase chain reaction RNA was extracted using the TRIzol reagent (Invitrogen) and used for cDNA synthesis with a M-MLV Reverse Transcriptase (TaKaRa, Dalian, China). .. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) using the ABI Prism 7500 Real-time PCR System (Applied Biosystems).

    cDNA Library Assay:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Polymerase Chain Reaction:

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

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  • 99
    TaKaRa advantage rt for pcr kit
    <t>RT-PCR</t> on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.
    Advantage Rt For Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advantage rt for pcr kit/product/TaKaRa
    Average 99 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    advantage rt for pcr kit - by Bioz Stars, 2020-08
    99/100 stars
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    85
    TaKaRa moloney murine leukemia virus superscript ii reverse transcriptase
    <t>RT-PCR</t> on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.
    Moloney Murine Leukemia Virus Superscript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus superscript ii reverse transcriptase/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus superscript ii reverse transcriptase - by Bioz Stars, 2020-08
    85/100 stars
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    92
    TaKaRa moloney murine leukemia virus m mlv reverse transcriptase
    <t>RT-PCR</t> on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.
    Moloney Murine Leukemia Virus M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus m mlv reverse transcriptase/product/TaKaRa
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus m mlv reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    RT-PCR on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.

    Journal: The Journal of Neuroscience

    Article Title: M-Like K+ Currents in Type I Hair Cells and Calyx Afferent Endings of the Developing Rat Utricle

    doi: 10.1523/JNEUROSCI.2596-06.2006

    Figure Lengend Snippet: RT-PCR on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.

    Article Snippet: The utricular mRNA was reverse transcribed with oligo-dT primers and the Moloney murine leukemia virus reverse transcriptase (Advantage RT for PCR kit; Clontech, Mountain View, CA) or Superscript III reverse transcriptase (First-Strand Synthesis System; Invitrogen, Carlsbad, CA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Polymerase Chain Reaction

    PMGS is highly expressed during early stages of neuronal differentiation. A , Quantification of the relative enrichment of PMGS mRNA in four independent RT-PCR experiments. Semiquantitative RT-PCR analysis shows that PMGS is significantly more expressed in embryonic than in adult hippocampi. Each gray bar represents the ratio of PMGS mRNA concentration in embryonic hippocampi ( E15 ) over that found in adult hippocampi (postnatal day 60) for a given experiment, as measured by densitometric analysis of the band intensities. The mean is indicated by the black bar . Error bar corresponds to the SD. The bottom panel shows the result of a representative experiment. The expression level of the housekeeping gene GAPDH was used to normalize the mRNA concentration. B , Western blot using polyclonal anti-PMGS antibody of membrane extracts from hippocampal neurons grown on PLL or on laminin-coated dishes. Neurons grown on laminin express much higher levels of PMGS than those grown on PLL. In this case the higher expression occurs after 3 d in culture. A second band (labeled a ) of ∼5 kDa higher molecular weight could represent a post-translational modification. The two lanes labeled preimm. show the reprobing with preimmune serum of the blot for laminin-grown cells. This demonstrates that the bottom band (labeled b ) is nonspecific. In the right lane ( HA ), we localized the PMGS band by probing membranes from HA–PMGS-overexpressing neurons with anti-HA antibody. C , Quantification of PMGS activity in membranes from PLL- and laminin-grown cells. Sialic acid release from a mixture of oligosialogangliosides of such confirmed the high activity of the enzyme in laminin-grown neurons.

    Journal: The Journal of Neuroscience

    Article Title: Plasma Membrane Ganglioside Sialidase Regulates Axonal Growth and Regeneration in Hippocampal Neurons in Culture

    doi: 10.1523/JNEUROSCI.21-21-08387.2001

    Figure Lengend Snippet: PMGS is highly expressed during early stages of neuronal differentiation. A , Quantification of the relative enrichment of PMGS mRNA in four independent RT-PCR experiments. Semiquantitative RT-PCR analysis shows that PMGS is significantly more expressed in embryonic than in adult hippocampi. Each gray bar represents the ratio of PMGS mRNA concentration in embryonic hippocampi ( E15 ) over that found in adult hippocampi (postnatal day 60) for a given experiment, as measured by densitometric analysis of the band intensities. The mean is indicated by the black bar . Error bar corresponds to the SD. The bottom panel shows the result of a representative experiment. The expression level of the housekeeping gene GAPDH was used to normalize the mRNA concentration. B , Western blot using polyclonal anti-PMGS antibody of membrane extracts from hippocampal neurons grown on PLL or on laminin-coated dishes. Neurons grown on laminin express much higher levels of PMGS than those grown on PLL. In this case the higher expression occurs after 3 d in culture. A second band (labeled a ) of ∼5 kDa higher molecular weight could represent a post-translational modification. The two lanes labeled preimm. show the reprobing with preimmune serum of the blot for laminin-grown cells. This demonstrates that the bottom band (labeled b ) is nonspecific. In the right lane ( HA ), we localized the PMGS band by probing membranes from HA–PMGS-overexpressing neurons with anti-HA antibody. C , Quantification of PMGS activity in membranes from PLL- and laminin-grown cells. Sialic acid release from a mixture of oligosialogangliosides of such confirmed the high activity of the enzyme in laminin-grown neurons.

    Article Snippet: Poly(A+ ) RNA was affinity purified with the QuickPrep Micro mRNA purification kit (Amersham Pharmacia Biotech, Uppsala, Sweden) from isolated hippocampi [both from embryonic day (E) 15 embryos and from 2-month-old adult mice]. cDNA synthesis was performed using ∼10 ng of mRNA and the Moloney murine leukemia virus reverse transcriptase (Advantage RT-for-PCR kit, Clontech Laboratories, Palo Alto, CA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Expressing, Western Blot, Labeling, Molecular Weight, Modification, Activity Assay