moloney murine leukemia virus reverse transcriptase  (TaKaRa)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    MMLV Reverse Transcriptase
    Description:
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    Catalog Number:
    639575
    Price:
    None
    Size:
    8 000 Units
    Category:
    MMLV RT GPR Reverse transcriptases cDNA synthesis
    Buy from Supplier


    Structured Review

    TaKaRa moloney murine leukemia virus reverse transcriptase
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2021-01
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: ZIP4, a Novel Determinant of Tumor Invasion in Hepatocellular Carcinoma, Contributes to Tumor Recurrence after Liver Transplantation
    Article Snippet: .. Real-time polymerase chain reaction RNA was extracted using the TRIzol reagent (Invitrogen) and used for cDNA synthesis with a M-MLV Reverse Transcriptase (TaKaRa, Dalian, China). .. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) using the ABI Prism 7500 Real-time PCR System (Applied Biosystems).

    Article Title: Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
    Article Snippet: .. Because of the increased presence of bacterial RNA during the daily population regrowth and based on preliminary RNA quantifications, total RNA quantities (500 µg) were adjusted for cDNA synthesis by a factor of 1.1 for the WT condition at 11 hdp and by a factor of 1.2 at 23 hpd. qRT-PCR procedures conform to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines ( ) and follow already-published protocols , with the following specifications. cDNAs were synthetized by the SMART Moloney murine leukemia virus (MMLV) reverse transcriptase (Clontech) from oligo(dT)12–18 primers (Life Technologies) and diluted 1/8-fold in water. qPCR mixes, consisting of 0.5 μl of each primer (10 μM), 5 μl of SsoAdvanced SYBR Green supermix (Bio-Rad), and 4 μl of a 1/8 dilution of the cDNA reaction mixture, were run on a CFX Bio-Rad instrument (2 technical replicates/biological replicate) as follows: 3 min at 94°C and 40 cycles of 10 s at 94°C, 10 s at 60°C, and 15 s at 72°C, with a melting curve from 70°C to 95°C. .. Primer sets (see ) exhibit PCR efficiencies between 92.3 and 101.9 (mean ± standard error [SE] = 97.5 ± 0.33), which were used to calculate expression values based on Pfaffl’s method ( ).

    Sequencing:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Agarose Gel Electrophoresis:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    Synthesized:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    Isolation:

    Article Title: Role of Heat Shock Proteases in Quorum-Sensing-Mediated Regulation of Biofilm Formation by Vibrio Species
    Article Snippet: .. Reverse transcription reactions were performed with the total RNA isolated from V. vulnificus , which had been harvested at several incubation time points, using Moloney murine leukemia virus (M-MLV) reverse transcriptase and a random 6-mer primer (TaKaRa). .. The cDNA was analyzed by quantitative RT-PCR using a LightCycler 480 II system (Roche).

    Spectrophotometry:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    SYBR Green Assay:

    Article Title: Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
    Article Snippet: .. Because of the increased presence of bacterial RNA during the daily population regrowth and based on preliminary RNA quantifications, total RNA quantities (500 µg) were adjusted for cDNA synthesis by a factor of 1.1 for the WT condition at 11 hdp and by a factor of 1.2 at 23 hpd. qRT-PCR procedures conform to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines ( ) and follow already-published protocols , with the following specifications. cDNAs were synthetized by the SMART Moloney murine leukemia virus (MMLV) reverse transcriptase (Clontech) from oligo(dT)12–18 primers (Life Technologies) and diluted 1/8-fold in water. qPCR mixes, consisting of 0.5 μl of each primer (10 μM), 5 μl of SsoAdvanced SYBR Green supermix (Bio-Rad), and 4 μl of a 1/8 dilution of the cDNA reaction mixture, were run on a CFX Bio-Rad instrument (2 technical replicates/biological replicate) as follows: 3 min at 94°C and 40 cycles of 10 s at 94°C, 10 s at 60°C, and 15 s at 72°C, with a melting curve from 70°C to 95°C. .. Primer sets (see ) exhibit PCR efficiencies between 92.3 and 101.9 (mean ± standard error [SE] = 97.5 ± 0.33), which were used to calculate expression values based on Pfaffl’s method ( ).

    Concentration Assay:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    Incubation:

    Article Title: Role of Heat Shock Proteases in Quorum-Sensing-Mediated Regulation of Biofilm Formation by Vibrio Species
    Article Snippet: .. Reverse transcription reactions were performed with the total RNA isolated from V. vulnificus , which had been harvested at several incubation time points, using Moloney murine leukemia virus (M-MLV) reverse transcriptase and a random 6-mer primer (TaKaRa). .. The cDNA was analyzed by quantitative RT-PCR using a LightCycler 480 II system (Roche).

    Quantitative RT-PCR:

    Article Title: Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
    Article Snippet: .. Because of the increased presence of bacterial RNA during the daily population regrowth and based on preliminary RNA quantifications, total RNA quantities (500 µg) were adjusted for cDNA synthesis by a factor of 1.1 for the WT condition at 11 hdp and by a factor of 1.2 at 23 hpd. qRT-PCR procedures conform to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines ( ) and follow already-published protocols , with the following specifications. cDNAs were synthetized by the SMART Moloney murine leukemia virus (MMLV) reverse transcriptase (Clontech) from oligo(dT)12–18 primers (Life Technologies) and diluted 1/8-fold in water. qPCR mixes, consisting of 0.5 μl of each primer (10 μM), 5 μl of SsoAdvanced SYBR Green supermix (Bio-Rad), and 4 μl of a 1/8 dilution of the cDNA reaction mixture, were run on a CFX Bio-Rad instrument (2 technical replicates/biological replicate) as follows: 3 min at 94°C and 40 cycles of 10 s at 94°C, 10 s at 60°C, and 15 s at 72°C, with a melting curve from 70°C to 95°C. .. Primer sets (see ) exhibit PCR efficiencies between 92.3 and 101.9 (mean ± standard error [SE] = 97.5 ± 0.33), which were used to calculate expression values based on Pfaffl’s method ( ).

    cDNA Library Assay:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa advantage rt for pcr kit
    <t>RT-PCR</t> on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.
    Advantage Rt For Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advantage rt for pcr kit/product/TaKaRa
    Average 99 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    advantage rt for pcr kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    TaKaRa moloney murine leukemia virus m mlv reverse transcriptase
    <t>RT-PCR</t> on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.
    Moloney Murine Leukemia Virus M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus m mlv reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus m mlv reverse transcriptase - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    RT-PCR on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.

    Journal: The Journal of Neuroscience

    Article Title: M-Like K+ Currents in Type I Hair Cells and Calyx Afferent Endings of the Developing Rat Utricle

    doi: 10.1523/JNEUROSCI.2596-06.2006

    Figure Lengend Snippet: RT-PCR on single cells revealed widespread expression of KCNQ and erg subunits. A , Agarose gels showing PCR products from P8 rat utricular hair cells for KCNQ3, KCNQ4, and KCNQ5 (top) and erg1, erg2, and erg3 (bottom). Top, Nine type I hair cells from one macula were probed for KCNQ3–KCNQ5. Six cells (3, 4, 5, 6, 8, 9) were positive for KCNQ3 (160 bp). Seven cells (1, 2, 3, 4, 5, 7, 8) were positive for KCNQ5 (106 bp). The band for cell 1 was faint. The identities of products at other molecular weights are not known. None were positive for KCNQ4 (380 bp). Bottom, Six type I hair cells from another macula were probed for erg1, erg2, and erg3. Cell 2 was positive for all three subunits, cell 4 was positive for erg2 and erg3 (the band corresponding to erg2 is faint), and five of six cells (cells 1–5) were positive for erg3. B , Percentages of positive PCR products for the following: left, single type I hair cells tested with erg1, erg2, erg3, KCNQ3, KCNQ4, and KCNQ5 probes at P1 and P8, and with erg1, erg2, erg 3, and KCNQ4 probes at P14; right, single type II cells tested with KCNQ3 and KCNQ5 probes at P8. We did not test type II cells at other ages, nor did we test them for KCNQ4 at any age. The number of cells tested in each condition is given at the top of the histogram bars.

    Article Snippet: The utricular mRNA was reverse transcribed with oligo-dT primers and the Moloney murine leukemia virus reverse transcriptase (Advantage RT for PCR kit; Clontech, Mountain View, CA) or Superscript III reverse transcriptase (First-Strand Synthesis System; Invitrogen, Carlsbad, CA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Polymerase Chain Reaction

    PMGS is highly expressed during early stages of neuronal differentiation. A , Quantification of the relative enrichment of PMGS mRNA in four independent RT-PCR experiments. Semiquantitative RT-PCR analysis shows that PMGS is significantly more expressed in embryonic than in adult hippocampi. Each gray bar represents the ratio of PMGS mRNA concentration in embryonic hippocampi ( E15 ) over that found in adult hippocampi (postnatal day 60) for a given experiment, as measured by densitometric analysis of the band intensities. The mean is indicated by the black bar . Error bar corresponds to the SD. The bottom panel shows the result of a representative experiment. The expression level of the housekeeping gene GAPDH was used to normalize the mRNA concentration. B , Western blot using polyclonal anti-PMGS antibody of membrane extracts from hippocampal neurons grown on PLL or on laminin-coated dishes. Neurons grown on laminin express much higher levels of PMGS than those grown on PLL. In this case the higher expression occurs after 3 d in culture. A second band (labeled a ) of ∼5 kDa higher molecular weight could represent a post-translational modification. The two lanes labeled preimm. show the reprobing with preimmune serum of the blot for laminin-grown cells. This demonstrates that the bottom band (labeled b ) is nonspecific. In the right lane ( HA ), we localized the PMGS band by probing membranes from HA–PMGS-overexpressing neurons with anti-HA antibody. C , Quantification of PMGS activity in membranes from PLL- and laminin-grown cells. Sialic acid release from a mixture of oligosialogangliosides of such confirmed the high activity of the enzyme in laminin-grown neurons.

    Journal: The Journal of Neuroscience

    Article Title: Plasma Membrane Ganglioside Sialidase Regulates Axonal Growth and Regeneration in Hippocampal Neurons in Culture

    doi: 10.1523/JNEUROSCI.21-21-08387.2001

    Figure Lengend Snippet: PMGS is highly expressed during early stages of neuronal differentiation. A , Quantification of the relative enrichment of PMGS mRNA in four independent RT-PCR experiments. Semiquantitative RT-PCR analysis shows that PMGS is significantly more expressed in embryonic than in adult hippocampi. Each gray bar represents the ratio of PMGS mRNA concentration in embryonic hippocampi ( E15 ) over that found in adult hippocampi (postnatal day 60) for a given experiment, as measured by densitometric analysis of the band intensities. The mean is indicated by the black bar . Error bar corresponds to the SD. The bottom panel shows the result of a representative experiment. The expression level of the housekeeping gene GAPDH was used to normalize the mRNA concentration. B , Western blot using polyclonal anti-PMGS antibody of membrane extracts from hippocampal neurons grown on PLL or on laminin-coated dishes. Neurons grown on laminin express much higher levels of PMGS than those grown on PLL. In this case the higher expression occurs after 3 d in culture. A second band (labeled a ) of ∼5 kDa higher molecular weight could represent a post-translational modification. The two lanes labeled preimm. show the reprobing with preimmune serum of the blot for laminin-grown cells. This demonstrates that the bottom band (labeled b ) is nonspecific. In the right lane ( HA ), we localized the PMGS band by probing membranes from HA–PMGS-overexpressing neurons with anti-HA antibody. C , Quantification of PMGS activity in membranes from PLL- and laminin-grown cells. Sialic acid release from a mixture of oligosialogangliosides of such confirmed the high activity of the enzyme in laminin-grown neurons.

    Article Snippet: Poly(A+ ) RNA was affinity purified with the QuickPrep Micro mRNA purification kit (Amersham Pharmacia Biotech, Uppsala, Sweden) from isolated hippocampi [both from embryonic day (E) 15 embryos and from 2-month-old adult mice]. cDNA synthesis was performed using ∼10 ng of mRNA and the Moloney murine leukemia virus reverse transcriptase (Advantage RT-for-PCR kit, Clontech Laboratories, Palo Alto, CA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Expressing, Western Blot, Labeling, Molecular Weight, Modification, Activity Assay