moloney murine leukemia virus reverse transcriptase  (Promega)

 
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    Promega moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Promega
    Average 94 stars, based on 939 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *"

    Article Title: Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.403881

    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Figure Legend Snippet: Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Techniques Used: Transduction, Concentration Assay, Clone Assay, Real-time Polymerase Chain Reaction, Expressing, Staining, FACS, Infection

    2) Product Images from "Somatostatin stimulates intestinal NHE8 expression via p38 MAPK pathway"

    Article Title: Somatostatin stimulates intestinal NHE8 expression via p38 MAPK pathway

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00421.2010

    Expression of intestinal SST receptors (SSTRs) in mice and Caco-2 cells. Total RNA was isolated from mouse intestinal mucosa and Caco-2 cells. Reverse transcription reaction was performed with Moloney murine leukemia virus reverse transcriptase and random primers. RT-PCR was used to amplify SSTR cDNAs with SSRT gene-specific primers. PCR products were separated on 1.5% agarose gel and visualized after ethidium bromide staining. A : PCR amplification of SSTRs from mouse intestine. B : PCR amplification of SSTRs from Caco-2 cells.
    Figure Legend Snippet: Expression of intestinal SST receptors (SSTRs) in mice and Caco-2 cells. Total RNA was isolated from mouse intestinal mucosa and Caco-2 cells. Reverse transcription reaction was performed with Moloney murine leukemia virus reverse transcriptase and random primers. RT-PCR was used to amplify SSTR cDNAs with SSRT gene-specific primers. PCR products were separated on 1.5% agarose gel and visualized after ethidium bromide staining. A : PCR amplification of SSTRs from mouse intestine. B : PCR amplification of SSTRs from Caco-2 cells.

    Techniques Used: Expressing, Mouse Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Amplification

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Expression of genes of the aflatoxin biosynthetic pathway in Aspergillus flavus isolates from keratitis
    Article Snippet: .. cDNA synthesis and PCR amplification Total RNA was used as the template to generate first strand cDNA in a 20 µl reaction volume as follows: 2 µg of total RNA were added to 1 µl of 10 mM dNTPs and 2 µl of 100 µM oligo dTs, made up to 10 µl with RNase-free water, heated at 70 °C for 10 min and added to a reaction mix containing 2 µl of 10× reverse transcriptase buffer, 1 µl of Moloney murine leukemia virus reverse transcriptase (M-MLV RT) enzyme (Promega, Madison, WI) and RNase-free water. .. The reaction mixture was incubated at 37 °C for 60 min and terminated at 95 °C for 5 min. PCR amplication of the cDNAs of the genes being studied (afl R, afl J, nor-1, pks A) and of a `housekeeping’ gene (β-tubulin [TUB ]) was performed with a total reaction volume of 50 µl consisting of PCR buffer (1×), 0.2 mM each of dATP, dGTP, dCTP and dTTP, 0.5 µM of each primer ( ) and 1.5 µl of Taq DNA polymerase.

    Random Hexamer Labeling:

    Article Title: Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *
    Article Snippet: .. About 1.5 μg of RNA was used to synthesize cDNA using Random Hexamer Primer and Moloney murine leukemia virus reverse transcriptase (Promega) according to the manufacturer's protocol. .. Real-time PCR was performed on a Stratagene Mx 3000P thermal cycler using JumpStartTM TaqReady MixTM (Sigma, D7440) supplemented with Eva Green (Biotium).

    Amplification:

    Article Title: Expression of genes of the aflatoxin biosynthetic pathway in Aspergillus flavus isolates from keratitis
    Article Snippet: .. cDNA synthesis and PCR amplification Total RNA was used as the template to generate first strand cDNA in a 20 µl reaction volume as follows: 2 µg of total RNA were added to 1 µl of 10 mM dNTPs and 2 µl of 100 µM oligo dTs, made up to 10 µl with RNase-free water, heated at 70 °C for 10 min and added to a reaction mix containing 2 µl of 10× reverse transcriptase buffer, 1 µl of Moloney murine leukemia virus reverse transcriptase (M-MLV RT) enzyme (Promega, Madison, WI) and RNase-free water. .. The reaction mixture was incubated at 37 °C for 60 min and terminated at 95 °C for 5 min. PCR amplication of the cDNAs of the genes being studied (afl R, afl J, nor-1, pks A) and of a `housekeeping’ gene (β-tubulin [TUB ]) was performed with a total reaction volume of 50 µl consisting of PCR buffer (1×), 0.2 mM each of dATP, dGTP, dCTP and dTTP, 0.5 µM of each primer ( ) and 1.5 µl of Taq DNA polymerase.

    Synthesized:

    Article Title: Transcriptome Analysis of Renal Ischemia/Reperfusion Injury and Its Modulation by Ischemic Pre-Conditioning or Hemin Treatment
    Article Snippet: .. First-strand cDNA was synthesized from DNAse-treated total RNA using Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Wisconsin, US). .. We used Taqman-based quantitative RT-PCR for validation of some of the genes identified by the microarray analysis.

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    Promega moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Promega
    Average 94 stars, based on 939 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    85
    Promega random oligonucleotide hexamer primer moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Random Oligonucleotide Hexamer Primer Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random oligonucleotide hexamer primer moloney murine leukemia virus reverse transcriptase/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    random oligonucleotide hexamer primer moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *

    doi: 10.1074/jbc.M112.403881

    Figure Lengend Snippet: Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Article Snippet: About 1.5 μg of RNA was used to synthesize cDNA using Random Hexamer Primer and Moloney murine leukemia virus reverse transcriptase (Promega) according to the manufacturer's protocol.

    Techniques: Transduction, Concentration Assay, Clone Assay, Real-time Polymerase Chain Reaction, Expressing, Staining, FACS, Infection

    Expression of intestinal SST receptors (SSTRs) in mice and Caco-2 cells. Total RNA was isolated from mouse intestinal mucosa and Caco-2 cells. Reverse transcription reaction was performed with Moloney murine leukemia virus reverse transcriptase and random primers. RT-PCR was used to amplify SSTR cDNAs with SSRT gene-specific primers. PCR products were separated on 1.5% agarose gel and visualized after ethidium bromide staining. A : PCR amplification of SSTRs from mouse intestine. B : PCR amplification of SSTRs from Caco-2 cells.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Somatostatin stimulates intestinal NHE8 expression via p38 MAPK pathway

    doi: 10.1152/ajpcell.00421.2010

    Figure Lengend Snippet: Expression of intestinal SST receptors (SSTRs) in mice and Caco-2 cells. Total RNA was isolated from mouse intestinal mucosa and Caco-2 cells. Reverse transcription reaction was performed with Moloney murine leukemia virus reverse transcriptase and random primers. RT-PCR was used to amplify SSTR cDNAs with SSRT gene-specific primers. PCR products were separated on 1.5% agarose gel and visualized after ethidium bromide staining. A : PCR amplification of SSTRs from mouse intestine. B : PCR amplification of SSTRs from Caco-2 cells.

    Article Snippet: Total RNA (500 ng) was reverse transcribed to cDNA by Moloney murine leukemia virus reverse transcriptase (Promega).

    Techniques: Expressing, Mouse Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Amplification