moloney murine leukemia virus reverse transcriptase  (Promega)

 
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    Promega moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Promega
    Average 99 stars, based on 939 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2021-01
    99/100 stars

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    RT Lamp Assay:

    Article Title: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
    Article Snippet: .. For each primer set, AMV and M-MLV RT-LAMP was performed and similar results were obtained, therefore, M-MLV reverse transcriptase was chosen for the subsequent RT-LAMP assay because of its relatively cheap price (Figure ). ..

    Purification:

    Article Title: APOBEC3A deaminates transiently exposed single-strand DNA during LINE-1 retrotransposition
    Article Snippet: .. RT-PCR was carried out on 0.5 μl of purified RNA, using MMLV reverse transcriptase (Promega, Madison, WI) and 0.8 μM LEAP adapter (5np1) at 42°C for 30 min. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: OsTIR1 and OsAFB2 Downregulation via OsmiR393 Overexpression Leads to More Tillers, Early Flowering and Less Tolerance to Salt and Drought in Rice
    Article Snippet: .. The total RNA reverse transcription reaction was performed with a two-step RT-PCR kit (Promega, Cat.# M 1701) on 2 µg of total RNA according to the product manual. .. Expression detection by real-time quantitative PCR Gene expression was analyzed by quantitative real time RT-PCR and semi-quantitative RT-PCR using the primers listed in .

    Article Title: APOBEC3A deaminates transiently exposed single-strand DNA during LINE-1 retrotransposition
    Article Snippet: .. RT-PCR was carried out on 0.5 μl of purified RNA, using MMLV reverse transcriptase (Promega, Madison, WI) and 0.8 μM LEAP adapter (5np1) at 42°C for 30 min. ..

    Incubation:

    Article Title: A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing
    Article Snippet: .. After incubation, 4 μl of the RT buffer, 1 μl of 0.1 M DTT, 5 U RNasin and 0.5 μl M-MLV reverse transcriptase (Promega) were supplemented and reacted at 25°C for 30 min. ..

    other:

    Article Title: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
    Article Snippet: Discussion WYMV could be detected by the RT-LAMP method using the designed four sets of primers (I-IV) and M-MLV reverse transcriptase instead of AMV reverse transcriptase under isothermal condition at 65°C for 80 min.

    Polymerase Chain Reaction:

    Article Title: Calcium-dependent regulation of the cell cycle via a novel MAPK-NF-?B pathway in Swiss 3T3 cells
    Article Snippet: .. 2 μg of RNA was used to perform reverse transcriptase PCR (using 200 U M-MLV reverse transcriptase; Promega). .. For PCR amplification, 5 μl of RT products were incubated with 20 pmol of specific primers and 1 U of Taq DNA polymerase (Promega).

    Clear Native PAGE:

    Article Title: Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis
    Article Snippet: .. Native PAGE assays Five picomoles of RNAP and 25 pmol of HelD, T4 DNA ligase (TaKaRa) or MLV reverse transcriptase (Promega), respectively, were used. .. Proteins tested for mutual interactions were incubated for 15 min at 30°C in 10 μl in the storage buffer.

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    Promega moloney murine leukemia virus reverse transcriptase
    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a <t>Moloney</t> murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Promega
    Average 99 stars, based on 939 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2021-01
    99/100 stars
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    Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency *

    doi: 10.1074/jbc.M112.403881

    Figure Lengend Snippet: Higher proliferation rate of MEFs correlates with lower reprogramming efficiency. A and B, time course study of the proliferation rate of MEFs and the reprogramming efficiency with OSK and serially diluted c- Myc viruses. MEFs were transduced with OSK plus c-Myc in 5-fold serial dilutions (including zero concentration). The number of total cells ( A ) and the percentage of GFP + cells ( B ) were monitored every other day during the reprogramming process. C, proliferation rates of two OSK-iPS and two OSKM-iPS clones. Cell numbers were normalized to the value obtained on day 1. D, quantitative PCR analysis of the expression levels of Cdk 1 / 2 / 4 in OSK or OSKM iPS clones. The expression level of each gene was normalized to that of β -actin in the same sample and then normalized to MEFs. E, characterization of cell death in OSK- or OSKM-mediated reprogramming using propidium iodide staining and FACS analysis. F and G , MEFs were infected with OSKM or OSK in combination with a Moloney murine leukemia virus 5′ LTR promoter-driven dsRed virus and seeded at different densities in a 96-well plate, the number of GFP + dsRed − colonies were counted at day 14 ( G ), the number of dsRed + cells were recorded at day 6, and the reprogramming efficiency ( H ) was calculated (number of GFP + dsRed − colonies/number of dsRed + cells). *, p

    Article Snippet: About 1.5 μg of RNA was used to synthesize cDNA using Random Hexamer Primer and Moloney murine leukemia virus reverse transcriptase (Promega) according to the manufacturer's protocol.

    Techniques: Transduction, Concentration Assay, Clone Assay, Real-time Polymerase Chain Reaction, Expressing, Staining, FACS, Infection

    Expression of intestinal SST receptors (SSTRs) in mice and Caco-2 cells. Total RNA was isolated from mouse intestinal mucosa and Caco-2 cells. Reverse transcription reaction was performed with Moloney murine leukemia virus reverse transcriptase and random primers. RT-PCR was used to amplify SSTR cDNAs with SSRT gene-specific primers. PCR products were separated on 1.5% agarose gel and visualized after ethidium bromide staining. A : PCR amplification of SSTRs from mouse intestine. B : PCR amplification of SSTRs from Caco-2 cells.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Somatostatin stimulates intestinal NHE8 expression via p38 MAPK pathway

    doi: 10.1152/ajpcell.00421.2010

    Figure Lengend Snippet: Expression of intestinal SST receptors (SSTRs) in mice and Caco-2 cells. Total RNA was isolated from mouse intestinal mucosa and Caco-2 cells. Reverse transcription reaction was performed with Moloney murine leukemia virus reverse transcriptase and random primers. RT-PCR was used to amplify SSTR cDNAs with SSRT gene-specific primers. PCR products were separated on 1.5% agarose gel and visualized after ethidium bromide staining. A : PCR amplification of SSTRs from mouse intestine. B : PCR amplification of SSTRs from Caco-2 cells.

    Article Snippet: Total RNA (500 ng) was reverse transcribed to cDNA by Moloney murine leukemia virus reverse transcriptase (Promega).

    Techniques: Expressing, Mouse Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Amplification