moloney murine leukemia virus reverse transcriptase  (New England Biolabs)


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  • 99
    Name:
    M MuLV Reverse Transcriptase
    Description:
    M MuLV Reverse Transcriptase 50 000 units
    Catalog Number:
    m0253l
    Price:
    288
    Size:
    50 000 units
    Category:
    Reverse Transcriptases
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    New England Biolabs moloney murine leukemia virus reverse transcriptase
    M MuLV Reverse Transcriptase
    M MuLV Reverse Transcriptase 50 000 units
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/New England Biolabs
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB). .. RNA was combined with primers and annealed in water at 70°C for 5 minutes followed by incubation on ice.

    Produced:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. The length distribution of the 3173 Pol cDNAs was visibly shorter than that produced by the MMLV RT, although a subset of the 3173 extension products appeared to be so large that they barely entered the gel. .. Incubation of the DNA primer:RNA template complex with the Taq Pol negative control resulted in a structure-dependent 5′-3′ exonuclease cleavage product that migrated at the dye front .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB). .. RNA was combined with primers and annealed in water at 70°C for 5 minutes followed by incubation on ice.

    other:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: The 3173 Pol and MMLV RT were both able to extend the primer to produce faint, nearly full-length products although the 3173 Pol product was detectably longer than that of MMLV RT.

    Activity Assay:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. As an additional test to compare the RT activity of the 3173 Pol to that of MMLV-RT on a complex RNA substrate, a primer specific to bases 714 to 690 of the negative sense RNA MS2 genome was 5′-fluorophore-labeled. .. The labeled cDNA primer was extended using extracted MS2 RNA as a template ( ).

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Extension products from a 5′-fluorophore-labeled dT20 primer were resolved by denaturing polyacrylamide gel to further demonstrate RT activity and to assess the relative lengths of the extension products of the 3173 Pol and MMLV RT ( ). ..

    Sequencing:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Of the two longer target sequences tested, only the 821 bp beta-actin sequence (Lane C3) was reverse transcribed by the 3173 Pol and this synthesis appeared less efficient than that of MMLV RT. ..

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    New England Biolabs m mulv
    Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using <t>AMV</t> and <t>M-MuLV</t> (RNase H-) reverse transcriptases at 42°C for 15 h.
    M Mulv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv/product/New England Biolabs
    Average 99 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    m mulv - by Bioz Stars, 2020-09
    99/100 stars
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    Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using AMV and M-MuLV (RNase H-) reverse transcriptases at 42°C for 15 h.

    Journal: Nucleic Acids Research

    Article Title: Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes

    doi: 10.1093/nar/gkw028

    Figure Lengend Snippet: Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using AMV and M-MuLV (RNase H-) reverse transcriptases at 42°C for 15 h.

    Article Snippet: Klenow large fragment, Therminator™ II, M-MuLV (RNase H− ) reverse transcriptase, AMV reverse transcriptase, RNase inhibitor and λ-exonuclease were purchased from New England Biolabs.

    Techniques: Polyacrylamide Gel Electrophoresis

    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Journal: Journal of Advanced Research

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries

    doi: 10.1016/j.jare.2019.12.005

    Figure Lengend Snippet: Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Article Snippet: M−MuLV reverse transcriptase, ProtoScript® II Reverse Transcriptase, WarmStart RTx Reverse Transcriptase, RNase H, DNase I, Exonuclease I, Murine RNase Inhibitor, NEBNext® rRNA Depletion Kit (Human/Mouse/Rat), and NEBNext Ultra Directional RNA Library Prep Kit for Illumina were purchased from New England Biolabs (NEB, Ipswich, MA).

    Techniques: Expressing