moloney murine leukaemia virus  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukaemia virus
    Moloney Murine Leukaemia Virus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus/product/Thermo Fisher
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus - by Bioz Stars, 2020-05
    89/100 stars

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    Related Articles

    Produced:

    Article Title: T-bet-mediated differentiation of the activated CD8+ T cell
    Article Snippet: .. Moloney murine leukaemia virus expressing rat Thy1 alone (pMig-IRES-Thy1) or with T-bet (pMIG-T-bet-IRES-Thy1) was produced by transiently transfecting HEK293 cells at 90% confluency with an equimolar ratio of pMIG and the packaging construct, pCL-ECO with lipofectamine 2000 (Invitrogen). .. Primary CD8+ T cells were transduced 24 h after activation in the presence of IL-2, anti-IFN-γ, anti-IFN-γR1, anti-IL-12 and 6 μg/mL protamine sulphate (Sigma).

    Amplification:

    Article Title: Enhancement by histamine of vascular endothelial growth factor production in granulation tissue via H2 receptors
    Article Snippet: .. Two micrograms of RNA from each sample and 20 pmol of reverse primers of GAPDH and VEGF were incubated at 80°C for 5 min, cooled immediately and reverse-transcribed in 20 μl of a buffer (m M) : Tris-HCl (pH 8.3) 50, KCl 75, MgCl2 3, 200 units of the reverse-transcriptase from moloney murine leukaemia virus (Life Technologies, Gaithersburg, MD, U.S.A.), deoxyribonucleotide triphosphates (Amersham Pharmacia Biotech Co.) 0.5 and dithiothreitol 10, at 37°C for 1 h. For VEGF mRNA, PCR amplification was performed in 50 μl of a PCR buffer (Tris-HCl (pH 8.3) 2.5 m M , KCl 50 m M , MgCl2 1.5 m M , 2 μl of the reverse transcribed RNA solution, 20 pmol of each primer, 170 μ M dNTPs and 1.25 units of Taq polymerase (Takara Shuzo, Shiga, Japan)) with a thermal cycler (GeneAmp PCR system 2400, Perkin Elmer Cetus, Norwalk, CT, U.S.A.). ..

    Isolation:

    Article Title: Tacrolimus Increases Nox4 Expression in Human Renal Fibroblasts and Induces Fibrosis-Related Genes by Aberrant TGF-Beta Receptor Signalling
    Article Snippet: .. Real-time quantitative PCR (RT-qPCR) Total RNA was isolated by phenol extraction, using Tri Reagent (Sigma T9424) with standard laboratory techniques. mRNA was transcribed into cDNA using reverse transcriptase from Moloney Murine Leukaemia Virus (Invitrogen M-MLV-Kit, 28025-021). qRT-PCR was performed on an Applied Biosystems 7500 Fast RT qPCR System. .. The following pre-made assays by Applied Biosystems were used: NADPH-oxidase 4 (NOX4, Hs01558199_m1), Transgelin 1 (TAGLN, Hs00162558_m1), collagen alpha1-(V) (COL5A1, Hs00609088_m1), tropomyosin 1 (TPM1, Hs00165966_m1), smooth muscle alpha-actin (ACTA2, Hs00909449_m1), and transforming growth factor-β1 (TGFB1, Hs00171257_m1).

    Construct:

    Article Title: T-bet-mediated differentiation of the activated CD8+ T cell
    Article Snippet: .. Moloney murine leukaemia virus expressing rat Thy1 alone (pMig-IRES-Thy1) or with T-bet (pMIG-T-bet-IRES-Thy1) was produced by transiently transfecting HEK293 cells at 90% confluency with an equimolar ratio of pMIG and the packaging construct, pCL-ECO with lipofectamine 2000 (Invitrogen). .. Primary CD8+ T cells were transduced 24 h after activation in the presence of IL-2, anti-IFN-γ, anti-IFN-γR1, anti-IL-12 and 6 μg/mL protamine sulphate (Sigma).

    Real-time Polymerase Chain Reaction:

    Article Title: Tacrolimus Increases Nox4 Expression in Human Renal Fibroblasts and Induces Fibrosis-Related Genes by Aberrant TGF-Beta Receptor Signalling
    Article Snippet: .. Real-time quantitative PCR (RT-qPCR) Total RNA was isolated by phenol extraction, using Tri Reagent (Sigma T9424) with standard laboratory techniques. mRNA was transcribed into cDNA using reverse transcriptase from Moloney Murine Leukaemia Virus (Invitrogen M-MLV-Kit, 28025-021). qRT-PCR was performed on an Applied Biosystems 7500 Fast RT qPCR System. .. The following pre-made assays by Applied Biosystems were used: NADPH-oxidase 4 (NOX4, Hs01558199_m1), Transgelin 1 (TAGLN, Hs00162558_m1), collagen alpha1-(V) (COL5A1, Hs00609088_m1), tropomyosin 1 (TPM1, Hs00165966_m1), smooth muscle alpha-actin (ACTA2, Hs00909449_m1), and transforming growth factor-β1 (TGFB1, Hs00171257_m1).

    Polymerase Chain Reaction:

    Article Title: Enhancement by histamine of vascular endothelial growth factor production in granulation tissue via H2 receptors
    Article Snippet: .. Two micrograms of RNA from each sample and 20 pmol of reverse primers of GAPDH and VEGF were incubated at 80°C for 5 min, cooled immediately and reverse-transcribed in 20 μl of a buffer (m M) : Tris-HCl (pH 8.3) 50, KCl 75, MgCl2 3, 200 units of the reverse-transcriptase from moloney murine leukaemia virus (Life Technologies, Gaithersburg, MD, U.S.A.), deoxyribonucleotide triphosphates (Amersham Pharmacia Biotech Co.) 0.5 and dithiothreitol 10, at 37°C for 1 h. For VEGF mRNA, PCR amplification was performed in 50 μl of a PCR buffer (Tris-HCl (pH 8.3) 2.5 m M , KCl 50 m M , MgCl2 1.5 m M , 2 μl of the reverse transcribed RNA solution, 20 pmol of each primer, 170 μ M dNTPs and 1.25 units of Taq polymerase (Takara Shuzo, Shiga, Japan)) with a thermal cycler (GeneAmp PCR system 2400, Perkin Elmer Cetus, Norwalk, CT, U.S.A.). ..

    Incubation:

    Article Title: Enhancement by histamine of vascular endothelial growth factor production in granulation tissue via H2 receptors
    Article Snippet: .. Two micrograms of RNA from each sample and 20 pmol of reverse primers of GAPDH and VEGF were incubated at 80°C for 5 min, cooled immediately and reverse-transcribed in 20 μl of a buffer (m M) : Tris-HCl (pH 8.3) 50, KCl 75, MgCl2 3, 200 units of the reverse-transcriptase from moloney murine leukaemia virus (Life Technologies, Gaithersburg, MD, U.S.A.), deoxyribonucleotide triphosphates (Amersham Pharmacia Biotech Co.) 0.5 and dithiothreitol 10, at 37°C for 1 h. For VEGF mRNA, PCR amplification was performed in 50 μl of a PCR buffer (Tris-HCl (pH 8.3) 2.5 m M , KCl 50 m M , MgCl2 1.5 m M , 2 μl of the reverse transcribed RNA solution, 20 pmol of each primer, 170 μ M dNTPs and 1.25 units of Taq polymerase (Takara Shuzo, Shiga, Japan)) with a thermal cycler (GeneAmp PCR system 2400, Perkin Elmer Cetus, Norwalk, CT, U.S.A.). ..

    Quantitative RT-PCR:

    Article Title: Tacrolimus Increases Nox4 Expression in Human Renal Fibroblasts and Induces Fibrosis-Related Genes by Aberrant TGF-Beta Receptor Signalling
    Article Snippet: .. Real-time quantitative PCR (RT-qPCR) Total RNA was isolated by phenol extraction, using Tri Reagent (Sigma T9424) with standard laboratory techniques. mRNA was transcribed into cDNA using reverse transcriptase from Moloney Murine Leukaemia Virus (Invitrogen M-MLV-Kit, 28025-021). qRT-PCR was performed on an Applied Biosystems 7500 Fast RT qPCR System. .. The following pre-made assays by Applied Biosystems were used: NADPH-oxidase 4 (NOX4, Hs01558199_m1), Transgelin 1 (TAGLN, Hs00162558_m1), collagen alpha1-(V) (COL5A1, Hs00609088_m1), tropomyosin 1 (TPM1, Hs00165966_m1), smooth muscle alpha-actin (ACTA2, Hs00909449_m1), and transforming growth factor-β1 (TGFB1, Hs00171257_m1).

    Expressing:

    Article Title: T-bet-mediated differentiation of the activated CD8+ T cell
    Article Snippet: .. Moloney murine leukaemia virus expressing rat Thy1 alone (pMig-IRES-Thy1) or with T-bet (pMIG-T-bet-IRES-Thy1) was produced by transiently transfecting HEK293 cells at 90% confluency with an equimolar ratio of pMIG and the packaging construct, pCL-ECO with lipofectamine 2000 (Invitrogen). .. Primary CD8+ T cells were transduced 24 h after activation in the presence of IL-2, anti-IFN-γ, anti-IFN-γR1, anti-IL-12 and 6 μg/mL protamine sulphate (Sigma).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Molecular Mechanisms Leading to Null-protein Product from Retinoschisin (RS1) Signal-sequence Mutants in X-linked Retinoschisis (XLRS) Disease
    Article Snippet: .. Reverse transcription was carried out with 1 μg of RNA with Moloney Murine Leukaemia Virus (MMLV) based Reverse Transcriptase Kit (SuperScript One Step RT-PCR with Platinum® Taq , Invitrogen). .. All amplifications were primed by pairs of chemically synthesized 18–22-mer oligonucleotides designed with the RS1 transcript sequence ( ; primers 13–17).

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    Thermo Fisher moloney murine leukaemia virus reverse transcriptase
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    Thermo Fisher recombinant moloney murine leukemia virus reverse transcriptase
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    Thermo Fisher m mlv kit
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