moloney murine leukaemia virus reverse transcriptase  (Promega)

 
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    Promega moloney murine leukaemia virus reverse transcriptase
    Moloney Murine Leukaemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase/product/Promega
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase - by Bioz Stars, 2020-08
    99/100 stars

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    Amplification:

    Article Title: Effects of THAP11 on Erythroid Differentiation and Megakaryocytic Differentiation of K562 Cells
    Article Snippet: .. Reverse transcription-PCR (RT-PCR) and quantitative real-time RT-PCR Total RNA was reverse-transcribed and amplified using reverse transcription and PCR kits, respectively (Promega Corp., Madison, WI, U.S.). .. Real-time RT-PCR was performed by Bio-Rad IQ5 (Bio-Rad, U.S.).

    Quantitative RT-PCR:

    Article Title: Effects of THAP11 on Erythroid Differentiation and Megakaryocytic Differentiation of K562 Cells
    Article Snippet: .. Reverse transcription-PCR (RT-PCR) and quantitative real-time RT-PCR Total RNA was reverse-transcribed and amplified using reverse transcription and PCR kits, respectively (Promega Corp., Madison, WI, U.S.). .. Real-time RT-PCR was performed by Bio-Rad IQ5 (Bio-Rad, U.S.).

    Real-time Polymerase Chain Reaction:

    Article Title: Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways
    Article Snippet: .. Equal amounts of RNA (4 μg) was used for reverse-transcription PCR to prepare cDNA using M-MLV Reverse Transcriptase (Promega, USA), followed by PCR using rTaq DNA polymerase and quantitative real-time PCR using TransStart Green qPCR SuperMix (TransGen). .. The primers for ATMUV and chicken IFN-β, IFN-λ gene were designed using the Primer 5 software; other sequences of the primers used in this study have been described previously [ – ].

    Article Title: Cilia-related protein SPEF2 regulates osteoblast differentiation
    Article Snippet: .. Real-time PCR (RT-PCR) For analysis of gene expression with RT-PCR the total RNA was reverse transcribed with random primers and an RT-PCR kit (ImProm-II Reverse Transcription System, Promega) according to the manufacturer’s instructions. .. Produced cDNA was amplified by using gene specific primers listed in Supplemental Table .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Aspergillus fumigatus Survival in Alkaline and Extreme Zinc-Limiting Environments Relies on the Induction of a Zinc Homeostasis System Encoded by the zrfC and aspf2 Genes ▿ Genes ▿ †
    Article Snippet: .. The cDNA of zrfC was obtained by reverse transcription (RT)-PCR using oligonucleotides JA299 (for retrotranscription) and JA60 and JA54 (both for PCR), and a 1.6-kb cDNA fragment was subcloned into pGEM-T Easy (Promega) to generate plasmid pZRF30 and sequenced. .. The cDNA of aspf2 was obtained by RT-PCR using oligonucleotides JA8 (for retrotranscription) and JA166 and JA167 (both for PCR), and a 0.96-kb cDNA fragment was subcloned into pGEM-T Easy to generate plasmid pASPF23 and sequenced.

    Article Title: Restricted chromosomal silencing in nucleolar dominance
    Article Snippet: .. Reverse transcription (RT)-PCR was performed by using RNA that had been treated with RQ1 DNase I (Promega) to eliminate any contaminating genomic DNA. .. Total RNA was used in all cases except in the case of F16N15.13 , for which poly(A)+ RNA was isolated on oligo(dT) paramagnetic beads according to the manufacturer's instructions (Dynal, Great Neck, NY).

    Article Title: Effects of THAP11 on Erythroid Differentiation and Megakaryocytic Differentiation of K562 Cells
    Article Snippet: .. Reverse transcription-PCR (RT-PCR) and quantitative real-time RT-PCR Total RNA was reverse-transcribed and amplified using reverse transcription and PCR kits, respectively (Promega Corp., Madison, WI, U.S.). .. Real-time RT-PCR was performed by Bio-Rad IQ5 (Bio-Rad, U.S.).

    Article Title: Analysis of Transcription of the Staphylococcus aureus Aerobic Class Ib and Anaerobic Class III Ribonucleotide Reductase Genes in Response to Oxygen
    Article Snippet: .. For reverse transcription (RT)-PCR and primer extension, residual DNA was removed by treatment with RQ1 RNase-free DNase (Promega). .. RNA concentrations were determined by A 260 measurements, and RNA integrity was analyzed by agarose/formaldehyde gel electrophoresis ( ).

    Article Title: Virucidal nano-perforator of viral membrane trapping viral RNAs in the endosome
    Article Snippet: .. To confirm the release of vRNPs, reverse transcription (RT)-PCR was carried out using M-MLV reverse transcriptase (M1705, Promega, Madison, WI, USA) according to the manufacturer’s protocol using primers for the viral M gene of A/Puerto Rico/8/34; 5′-TGCACTTTGACATTGTGGATTCTTG-3′ (M_FW) and 5′-CCCTCATAGACTTTGGCACTCC-3′ (M_BW) . .. The coding region of the M sequence in the RT product was amplified with the described primers by using rTaq Plus 5 × PCR Master Mix (ELPIS-BIOTECH, Inc., Daejeon, Korea) according to the manufacturer’s protocol under the following thermal cycling conditions: 30 cycles of 95 °C for 10 s, 56 °C for 10 s, and 72 °C for 10 s. PCR products were separated by electrophoresis at 135 V for 20 min on a 1% agarose (Cosmogenetech, Inc., Korea) gel and visualised with a UV transilluminator.

    Article Title: Cilia-related protein SPEF2 regulates osteoblast differentiation
    Article Snippet: .. Real-time PCR (RT-PCR) For analysis of gene expression with RT-PCR the total RNA was reverse transcribed with random primers and an RT-PCR kit (ImProm-II Reverse Transcription System, Promega) according to the manufacturer’s instructions. .. Produced cDNA was amplified by using gene specific primers listed in Supplemental Table .

    Expressing:

    Article Title: Cilia-related protein SPEF2 regulates osteoblast differentiation
    Article Snippet: .. Real-time PCR (RT-PCR) For analysis of gene expression with RT-PCR the total RNA was reverse transcribed with random primers and an RT-PCR kit (ImProm-II Reverse Transcription System, Promega) according to the manufacturer’s instructions. .. Produced cDNA was amplified by using gene specific primers listed in Supplemental Table .

    Polymerase Chain Reaction:

    Article Title: Aspergillus fumigatus Survival in Alkaline and Extreme Zinc-Limiting Environments Relies on the Induction of a Zinc Homeostasis System Encoded by the zrfC and aspf2 Genes ▿ Genes ▿ †
    Article Snippet: .. The cDNA of zrfC was obtained by reverse transcription (RT)-PCR using oligonucleotides JA299 (for retrotranscription) and JA60 and JA54 (both for PCR), and a 1.6-kb cDNA fragment was subcloned into pGEM-T Easy (Promega) to generate plasmid pZRF30 and sequenced. .. The cDNA of aspf2 was obtained by RT-PCR using oligonucleotides JA8 (for retrotranscription) and JA166 and JA167 (both for PCR), and a 0.96-kb cDNA fragment was subcloned into pGEM-T Easy to generate plasmid pASPF23 and sequenced.

    Article Title: Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways
    Article Snippet: .. Equal amounts of RNA (4 μg) was used for reverse-transcription PCR to prepare cDNA using M-MLV Reverse Transcriptase (Promega, USA), followed by PCR using rTaq DNA polymerase and quantitative real-time PCR using TransStart Green qPCR SuperMix (TransGen). .. The primers for ATMUV and chicken IFN-β, IFN-λ gene were designed using the Primer 5 software; other sequences of the primers used in this study have been described previously [ – ].

    Article Title: Effects of THAP11 on Erythroid Differentiation and Megakaryocytic Differentiation of K562 Cells
    Article Snippet: .. Reverse transcription-PCR (RT-PCR) and quantitative real-time RT-PCR Total RNA was reverse-transcribed and amplified using reverse transcription and PCR kits, respectively (Promega Corp., Madison, WI, U.S.). .. Real-time RT-PCR was performed by Bio-Rad IQ5 (Bio-Rad, U.S.).

    Plasmid Preparation:

    Article Title: Aspergillus fumigatus Survival in Alkaline and Extreme Zinc-Limiting Environments Relies on the Induction of a Zinc Homeostasis System Encoded by the zrfC and aspf2 Genes ▿ Genes ▿ †
    Article Snippet: .. The cDNA of zrfC was obtained by reverse transcription (RT)-PCR using oligonucleotides JA299 (for retrotranscription) and JA60 and JA54 (both for PCR), and a 1.6-kb cDNA fragment was subcloned into pGEM-T Easy (Promega) to generate plasmid pZRF30 and sequenced. .. The cDNA of aspf2 was obtained by RT-PCR using oligonucleotides JA8 (for retrotranscription) and JA166 and JA167 (both for PCR), and a 0.96-kb cDNA fragment was subcloned into pGEM-T Easy to generate plasmid pASPF23 and sequenced.

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    Promega m mlv reverse transcriptase
    Agarose gel analysis of different RT-LAMP reactions . I-IV were four sets of primers; <t>AMV</t> was AMV-mediated RT-LAMP; <t>M-MLV</t> was M-MLV-mediated RT-LAMP. M: 100-bp DNA ladder marker; 1 and 2: healthy controls; 3 and 4: virus-infected samples
    M Mlv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1817 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv reverse transcriptase/product/Promega
    Average 99 stars, based on 1817 article reviews
    Price from $9.99 to $1999.99
    m mlv reverse transcriptase - by Bioz Stars, 2020-08
    99/100 stars
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    Agarose gel analysis of different RT-LAMP reactions . I-IV were four sets of primers; AMV was AMV-mediated RT-LAMP; M-MLV was M-MLV-mediated RT-LAMP. M: 100-bp DNA ladder marker; 1 and 2: healthy controls; 3 and 4: virus-infected samples

    Journal: Virology Journal

    Article Title: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification

    doi: 10.1186/1743-422X-8-550

    Figure Lengend Snippet: Agarose gel analysis of different RT-LAMP reactions . I-IV were four sets of primers; AMV was AMV-mediated RT-LAMP; M-MLV was M-MLV-mediated RT-LAMP. M: 100-bp DNA ladder marker; 1 and 2: healthy controls; 3 and 4: virus-infected samples

    Article Snippet: For each primer set, AMV and M-MLV RT-LAMP was performed and similar results were obtained, therefore, M-MLV reverse transcriptase was chosen for the subsequent RT-LAMP assay because of its relatively cheap price (Figure ).

    Techniques: Agarose Gel Electrophoresis, Marker, Infection

    NF-κB dependent transcription is essential for cell cycle progression. (A) Swiss 3T3 fibroblasts were serum starved for 24 h. They were then stimulated with 10% FCS for 18 h in the absence or presence of an NF-κB inhibitor (5 μM Bay117082). Cell cycle stage was determined by flow cytometry analysis of propidium iodide–stained cells. (B) Swiss 3T3 cells were transfected with the indicated reporter vectors (NF-κB luc and −1745 CD1-luc). 24 h after serum starvation, firefly luciferin (Biosynth) was added to the medium (0.5-mM final concentration). 2 h later, serum stimulation was performed and luminescence imaging was carried out using a Hamamatsu 4880-65 liquid nitrogen–cooled CCD camera. Images were acquired using 30-min integration times. (C) RT-PCR analysis was performed using primers specific to CD1 or cyclophilin A (control), with total RNA prepared from cells stimulated with 10% FCS at indicated time points. (D) RT-PCR analysis, with total RNA prepared from nonstimulated starved cells (ct) or cells stimulated 8 h with 10% FCS in the presence or absence of Bay117082.

    Journal: The Journal of Cell Biology

    Article Title: Calcium-dependent regulation of the cell cycle via a novel MAPK-NF-?B pathway in Swiss 3T3 cells

    doi: 10.1083/jcb.200402136

    Figure Lengend Snippet: NF-κB dependent transcription is essential for cell cycle progression. (A) Swiss 3T3 fibroblasts were serum starved for 24 h. They were then stimulated with 10% FCS for 18 h in the absence or presence of an NF-κB inhibitor (5 μM Bay117082). Cell cycle stage was determined by flow cytometry analysis of propidium iodide–stained cells. (B) Swiss 3T3 cells were transfected with the indicated reporter vectors (NF-κB luc and −1745 CD1-luc). 24 h after serum starvation, firefly luciferin (Biosynth) was added to the medium (0.5-mM final concentration). 2 h later, serum stimulation was performed and luminescence imaging was carried out using a Hamamatsu 4880-65 liquid nitrogen–cooled CCD camera. Images were acquired using 30-min integration times. (C) RT-PCR analysis was performed using primers specific to CD1 or cyclophilin A (control), with total RNA prepared from cells stimulated with 10% FCS at indicated time points. (D) RT-PCR analysis, with total RNA prepared from nonstimulated starved cells (ct) or cells stimulated 8 h with 10% FCS in the presence or absence of Bay117082.

    Article Snippet: 2 μg of RNA was used to perform reverse transcriptase PCR (using 200 U M-MLV reverse transcriptase; Promega).

    Techniques: Flow Cytometry, Cytometry, Staining, Transfection, Concentration Assay, Imaging, Reverse Transcription Polymerase Chain Reaction

    HelD interacts with RNAP core. ( A ) Coomassie stained gel of proteins purified by nickel affinity chromoatography. Lane 1—proteins purified from strain MH5636 containing His-tagged β’ subunit of RNAP; Lane 2—proteins purified from strain LK1401 containing His-tagged HelD; Lane 3—proteins purified from strain (BSB1) without any His-tagged protein. ( B ) Western blot of the gel from (A). The antibodies used are indicated. In the last lane purified σ A was used as a marker. ( C ) Band shift assay on native PAGE gel. RNAP was incubated with potential interacting partners: HelD, T4 DNA ligase (68 kDa), MLV reverse transcriptase (71 kDa). Samples were separated on 4–16% gradient native PAGE Bis–Tris gels. Lane 1—HelD, lane 2—RNAP, lane 3—HelD with RNAP, lane 4—DNaseI-treated HelD + DNaseI-treated RNAP, lane 5—T4 DNA ligase, lane 6—RNAP with T4 DNA ligase, lane 7—MLV reverse transcriptase, 6—RNAP with MLV reverse transcriptase. In all experiments, RNAP was prepared from the Δ helD strain (LK782).

    Journal: Nucleic Acids Research

    Article Title: Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis

    doi: 10.1093/nar/gku113

    Figure Lengend Snippet: HelD interacts with RNAP core. ( A ) Coomassie stained gel of proteins purified by nickel affinity chromoatography. Lane 1—proteins purified from strain MH5636 containing His-tagged β’ subunit of RNAP; Lane 2—proteins purified from strain LK1401 containing His-tagged HelD; Lane 3—proteins purified from strain (BSB1) without any His-tagged protein. ( B ) Western blot of the gel from (A). The antibodies used are indicated. In the last lane purified σ A was used as a marker. ( C ) Band shift assay on native PAGE gel. RNAP was incubated with potential interacting partners: HelD, T4 DNA ligase (68 kDa), MLV reverse transcriptase (71 kDa). Samples were separated on 4–16% gradient native PAGE Bis–Tris gels. Lane 1—HelD, lane 2—RNAP, lane 3—HelD with RNAP, lane 4—DNaseI-treated HelD + DNaseI-treated RNAP, lane 5—T4 DNA ligase, lane 6—RNAP with T4 DNA ligase, lane 7—MLV reverse transcriptase, 6—RNAP with MLV reverse transcriptase. In all experiments, RNAP was prepared from the Δ helD strain (LK782).

    Article Snippet: Native PAGE assays Five picomoles of RNAP and 25 pmol of HelD, T4 DNA ligase (TaKaRa) or MLV reverse transcriptase (Promega), respectively, were used.

    Techniques: Staining, Purification, Western Blot, Marker, Electrophoretic Mobility Shift Assay, Clear Native PAGE, Incubation

    VP5 facilitates RT initiation via a CPV panhandle. ( A ) Schematic illustrations of the predicted panhandle structure formed by 36 bases from the 5′-end and 30 bases from the 3′-end of HaCPV-5 RNA segment 8 (upper panel) and the primer complementary to the 3′-end of the panhandle (lower panel). ( B ) The RT reactions were conducted using the RNA panhandle structure and the DNA primer in the presence of M-MLV reverse transcriptase for 30 min at 25°C in the absence (lanes 1 and 4) or presence of MBP-VP5 (lane 2) or MBP alone (lane 3). For lane 1, the panhandle and primer were prehybridized via a thermal annealing treatment at 68°C before the reaction. ( C ) The RT reactions were conducted using the indicated amounts of RNA panhandles in the presence of indicated amounts of MBP-VP5 for 30 min at 25°C. The samples were analyzed on 6% urea PAGE, followed by northern blotting.

    Journal: Nucleic Acids Research

    Article Title: A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing

    doi: 10.1093/nar/gkt1256

    Figure Lengend Snippet: VP5 facilitates RT initiation via a CPV panhandle. ( A ) Schematic illustrations of the predicted panhandle structure formed by 36 bases from the 5′-end and 30 bases from the 3′-end of HaCPV-5 RNA segment 8 (upper panel) and the primer complementary to the 3′-end of the panhandle (lower panel). ( B ) The RT reactions were conducted using the RNA panhandle structure and the DNA primer in the presence of M-MLV reverse transcriptase for 30 min at 25°C in the absence (lanes 1 and 4) or presence of MBP-VP5 (lane 2) or MBP alone (lane 3). For lane 1, the panhandle and primer were prehybridized via a thermal annealing treatment at 68°C before the reaction. ( C ) The RT reactions were conducted using the indicated amounts of RNA panhandles in the presence of indicated amounts of MBP-VP5 for 30 min at 25°C. The samples were analyzed on 6% urea PAGE, followed by northern blotting.

    Article Snippet: After incubation, 4 μl of the RT buffer, 1 μl of 0.1 M DTT, 5 U RNasin and 0.5 μl M-MLV reverse transcriptase (Promega) were supplemented and reacted at 25°C for 30 min.

    Techniques: Polyacrylamide Gel Electrophoresis, Northern Blot