moloney murine leukaemia virus reverse transcriptase system  (Thermo Fisher)


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    Name:
    M MLV Reverse Transcriptase 200 U µL
    Description:
    M MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single stranded RNA DNA or an RNA DNA hybrid Compared to AMV RT Moloney Murine Leukemia Virus Reverse Transcriptase M MLV RT lacks DNA endonuclease activity and has a lower RNase H activity Features of this enzyme • Thermostability optimal activity at 37°C• Size of cDNA M MLV can be used to synthesize first strand cDNA up to 7 kb• Applications synthesis of first strand cDNA primer extension sequencing dsDNA cDNA libraries and RT PCRSourcePurified from E coli expressing the pol gene of M MLV on a plasmidPerformance and quality testingSDS PAGE purity endodeoxyribonuclease exodeoxyribonuclease and ribonuclease assays and yield and length of cDNA productUnit definitionOne unit of M MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid precipitable material in 10 min at 37°C using poly A oligo dT 25 as template primer Unit reaction conditions50 mM Tris HCl pH 8 3 40 mM KCl 6 mM MgCl2 1 mM DTT 0 5 mM 3H dTTP 0 1 mM poly A 0 1 mM oligo dT 25 0 1 mg mL BSA and enzyme in 50 µL for 10 min at 37°C
    Catalog Number:
    28025013
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher moloney murine leukaemia virus reverse transcriptase system
    M MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single stranded RNA DNA or an RNA DNA hybrid Compared to AMV RT Moloney Murine Leukemia Virus Reverse Transcriptase M MLV RT lacks DNA endonuclease activity and has a lower RNase H activity Features of this enzyme • Thermostability optimal activity at 37°C• Size of cDNA M MLV can be used to synthesize first strand cDNA up to 7 kb• Applications synthesis of first strand cDNA primer extension sequencing dsDNA cDNA libraries and RT PCRSourcePurified from E coli expressing the pol gene of M MLV on a plasmidPerformance and quality testingSDS PAGE purity endodeoxyribonuclease exodeoxyribonuclease and ribonuclease assays and yield and length of cDNA productUnit definitionOne unit of M MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid precipitable material in 10 min at 37°C using poly A oligo dT 25 as template primer Unit reaction conditions50 mM Tris HCl pH 8 3 40 mM KCl 6 mM MgCl2 1 mM DTT 0 5 mM 3H dTTP 0 1 mM poly A 0 1 mM oligo dT 25 0 1 mg mL BSA and enzyme in 50 µL for 10 min at 37°C
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase system - by Bioz Stars, 2020-12
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation
    Article Snippet: .. Reverse transcriptase PCR and quantitative real-time PCR analysis Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of CYP7A1 and SHP were analyzed by reverse transcriptase PCR (RT–PCR) or quantitative real-time PCR (qPCR) as indicated.

    Article Title: Orphan Nuclear Receptor Err? Induces C-Reactive Protein Gene Expression through Induction of ER-Bound Bzip Transmembrane Transcription Factor CREBH
    Article Snippet: .. Reverse Transcriptase PCR and Quantitative Real-time PCR Analyses Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of ERRγ, CREBH, CRP, and PGC1α were analyzed by reverse transcription PCR (RT–PCR) or quantitative real-time RT-PCR (qPCR) as indicated.

    Article Title: Transcriptional cross talk between orphan nuclear receptor ERR? and transmembrane transcription factor ATF6? coordinates endoplasmic reticulum stress response
    Article Snippet: .. Reverse transcriptase PCR and quantitative real-time PCR analysis Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of ATF6α and PGC1α were analyzed by RT-PCR or quantitative real-time RT-PCR (qPCR) as indicated.

    Article Title: Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant
    Article Snippet: .. cDNA was synthesized as described in “Reverse transcriptase PCR and quantitative real-time PCR.” Absolute quantification was performed using a QuantStudio 3D digital PCR system (Thermo Fisher Scientific) and analyzed with QuantStudio 3D AnalysisSuite cloud software (Thermo Fisher Scientific). .. The sequences of primers and probes were as follows: p62 full-length Left, CCCACAGGGCTGAAGGAA; p62 full-length Right, CATCTGGGAGAGGGACTCAATC; p62 full-length Probe, CCCACCAGAGGCTGA; p62 variant Left, CGATGACTGGACACATTTGTCTTC; p62 variant Right, TCTGGGAGAGGGACTCAATCA; and p62 full-length Probe, CCATCACAGAGGCTG.

    Synthesized:

    Article Title: Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant
    Article Snippet: .. cDNA was synthesized as described in “Reverse transcriptase PCR and quantitative real-time PCR.” Absolute quantification was performed using a QuantStudio 3D digital PCR system (Thermo Fisher Scientific) and analyzed with QuantStudio 3D AnalysisSuite cloud software (Thermo Fisher Scientific). .. The sequences of primers and probes were as follows: p62 full-length Left, CCCACAGGGCTGAAGGAA; p62 full-length Right, CATCTGGGAGAGGGACTCAATC; p62 full-length Probe, CCCACCAGAGGCTGA; p62 variant Left, CGATGACTGGACACATTTGTCTTC; p62 variant Right, TCTGGGAGAGGGACTCAATCA; and p62 full-length Probe, CCATCACAGAGGCTG.

    Isolation:

    Article Title: Both U2 snRNA and U12 snRNA are required for accurate splicing of exon 5 of the rat calcitonin/CGRP gene
    Article Snippet: .. Total RNA was isolated using Trizol (Invitrogen) and analyzed for splice products using reverse transcriptase–PCR. cDNAs were transcribed from RNA by Thermoscript reverse transcriptase (Invitrogen) using random hexamers as primers. .. PCR reactions were performed using 30 amplification cycles, the PCR products were extracted, separated by electrophoresis on 1.5% agarose gels, and visualized by ethidium bromide staining and under UV light.

    Article Title: Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation
    Article Snippet: .. Reverse transcriptase PCR and quantitative real-time PCR analysis Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of CYP7A1 and SHP were analyzed by reverse transcriptase PCR (RT–PCR) or quantitative real-time PCR (qPCR) as indicated.

    Article Title: Orphan Nuclear Receptor Err? Induces C-Reactive Protein Gene Expression through Induction of ER-Bound Bzip Transmembrane Transcription Factor CREBH
    Article Snippet: .. Reverse Transcriptase PCR and Quantitative Real-time PCR Analyses Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of ERRγ, CREBH, CRP, and PGC1α were analyzed by reverse transcription PCR (RT–PCR) or quantitative real-time RT-PCR (qPCR) as indicated.

    Article Title: Transcriptional cross talk between orphan nuclear receptor ERR? and transmembrane transcription factor ATF6? coordinates endoplasmic reticulum stress response
    Article Snippet: .. Reverse transcriptase PCR and quantitative real-time PCR analysis Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of ATF6α and PGC1α were analyzed by RT-PCR or quantitative real-time RT-PCR (qPCR) as indicated.

    Digital PCR:

    Article Title: Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant
    Article Snippet: .. cDNA was synthesized as described in “Reverse transcriptase PCR and quantitative real-time PCR.” Absolute quantification was performed using a QuantStudio 3D digital PCR system (Thermo Fisher Scientific) and analyzed with QuantStudio 3D AnalysisSuite cloud software (Thermo Fisher Scientific). .. The sequences of primers and probes were as follows: p62 full-length Left, CCCACAGGGCTGAAGGAA; p62 full-length Right, CATCTGGGAGAGGGACTCAATC; p62 full-length Probe, CCCACCAGAGGCTGA; p62 variant Left, CGATGACTGGACACATTTGTCTTC; p62 variant Right, TCTGGGAGAGGGACTCAATCA; and p62 full-length Probe, CCATCACAGAGGCTG.

    Incubation:

    Article Title: Double strand RNA delivery system for plant-sap-feeding insects
    Article Snippet: .. Reverse transcriptase PCR was used to generate cDNA, 200 ng of total RNA was incubated with a 0.5 mM deoxynucleoside triphosphate mixture, 0.65 μM each oligo(dT)16 (Life Technologies), and random hexamers (Life Technologies) at 65°C for 5 min. A cDNA synthesis mixture containing 10 mM dithiothreitol (DTT), 100 units of Superscript Reverse Transcriptase III (Life Technologies), and 2 units of SUPERase™ In RNase inhibitor (Life Technologies) was then added to the total RNA mixture, which was incubated at 25°C for 5 min, 50°C for 50 min. .. The reaction was terminated by incubation at 70°C for 15 min and the resulting cDNA was stored at -20°C.

    Polymerase Chain Reaction:

    Article Title: Double strand RNA delivery system for plant-sap-feeding insects
    Article Snippet: .. Reverse transcriptase PCR was used to generate cDNA, 200 ng of total RNA was incubated with a 0.5 mM deoxynucleoside triphosphate mixture, 0.65 μM each oligo(dT)16 (Life Technologies), and random hexamers (Life Technologies) at 65°C for 5 min. A cDNA synthesis mixture containing 10 mM dithiothreitol (DTT), 100 units of Superscript Reverse Transcriptase III (Life Technologies), and 2 units of SUPERase™ In RNase inhibitor (Life Technologies) was then added to the total RNA mixture, which was incubated at 25°C for 5 min, 50°C for 50 min. .. The reaction was terminated by incubation at 70°C for 15 min and the resulting cDNA was stored at -20°C.

    Article Title: Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation
    Article Snippet: .. Reverse transcriptase PCR and quantitative real-time PCR analysis Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of CYP7A1 and SHP were analyzed by reverse transcriptase PCR (RT–PCR) or quantitative real-time PCR (qPCR) as indicated.

    Article Title: Nucleocytosolic Depletion of the Energy Metabolite Acetyl-Coenzyme A Stimulates Autophagy and Prolongs Lifespan
    Article Snippet: .. Quantitative Reverse-Transcriptase PCR Target mRNA quantification by quantitative reverse-transcriptase PCR using ΔΔCt-method with 18S rRNA as an internal standard was performed on an ABI StepOnePlus using SYBR Select Master Mix (Life Tech, Invitrogen) ΔΔCt-method. .. Drosophila Lifespan Analyses and Brain Immunofluorescence UAS-RNAi lines for acetyl-CoA Synthetase (P{TRiP.HMS02314}attP2) and EGFP (P{VALIUM20-EGFP.shRNA.1}attP2) serving as a background-matched control were obtained from Bloomington Drosophila Stock Center.

    Article Title: Orphan Nuclear Receptor Err? Induces C-Reactive Protein Gene Expression through Induction of ER-Bound Bzip Transmembrane Transcription Factor CREBH
    Article Snippet: .. Reverse Transcriptase PCR and Quantitative Real-time PCR Analyses Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of ERRγ, CREBH, CRP, and PGC1α were analyzed by reverse transcription PCR (RT–PCR) or quantitative real-time RT-PCR (qPCR) as indicated.

    Article Title: Transcriptional cross talk between orphan nuclear receptor ERR? and transmembrane transcription factor ATF6? coordinates endoplasmic reticulum stress response
    Article Snippet: .. Reverse transcriptase PCR and quantitative real-time PCR analysis Total RNA was isolated using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. .. The mRNAs of ATF6α and PGC1α were analyzed by RT-PCR or quantitative real-time RT-PCR (qPCR) as indicated.

    Article Title: Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant
    Article Snippet: .. cDNA was synthesized as described in “Reverse transcriptase PCR and quantitative real-time PCR.” Absolute quantification was performed using a QuantStudio 3D digital PCR system (Thermo Fisher Scientific) and analyzed with QuantStudio 3D AnalysisSuite cloud software (Thermo Fisher Scientific). .. The sequences of primers and probes were as follows: p62 full-length Left, CCCACAGGGCTGAAGGAA; p62 full-length Right, CATCTGGGAGAGGGACTCAATC; p62 full-length Probe, CCCACCAGAGGCTGA; p62 variant Left, CGATGACTGGACACATTTGTCTTC; p62 variant Right, TCTGGGAGAGGGACTCAATCA; and p62 full-length Probe, CCATCACAGAGGCTG.

    Software:

    Article Title: Negative Regulation of the Keap1-Nrf2 Pathway by a p62/Sqstm1 Splicing Variant
    Article Snippet: .. cDNA was synthesized as described in “Reverse transcriptase PCR and quantitative real-time PCR.” Absolute quantification was performed using a QuantStudio 3D digital PCR system (Thermo Fisher Scientific) and analyzed with QuantStudio 3D AnalysisSuite cloud software (Thermo Fisher Scientific). .. The sequences of primers and probes were as follows: p62 full-length Left, CCCACAGGGCTGAAGGAA; p62 full-length Right, CATCTGGGAGAGGGACTCAATC; p62 full-length Probe, CCCACCAGAGGCTGA; p62 variant Left, CGATGACTGGACACATTTGTCTTC; p62 variant Right, TCTGGGAGAGGGACTCAATCA; and p62 full-length Probe, CCATCACAGAGGCTG.

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  • 99
    Thermo Fisher m mlv reverse transcriptase 200 u µl
    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total <t>RNA</t> extraction and <t>cDNA</t> synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P
    M Mlv Reverse Transcriptase 200 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv reverse transcriptase 200 u µl/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    m mlv reverse transcriptase 200 u µl - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher revertaid m mulv reverse transcriptase
    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total <t>RNA</t> extraction and <t>cDNA</t> synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P
    Revertaid M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 148 article reviews
    Price from $9.99 to $1999.99
    revertaid m mulv reverse transcriptase - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher recombinant moloney murine leukemia virus reverse transcriptase
    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total <t>RNA</t> extraction and <t>cDNA</t> synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P
    Recombinant Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-12
    88/100 stars
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    Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total RNA extraction and cDNA synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P

    Journal: Journal of Virology

    Article Title: Polymorphisms in the Most Oncolytic Reovirus Strain Confer Enhanced Cell Attachment, Transcription, and Single-Step Replication Kinetics

    doi: 10.1128/JVI.01937-19

    Figure Lengend Snippet: Postentry steps of reovirus replication are strongly enhanced for T3D PL . (A) T3D PL , T3D KC , and T3D TD (MOIs of 1 to 3) were bound to L929 cell monolayers at 4°C and following washing to remove unbound virions, cells were incubated at 37°C and cell lysates were collected at various time points postinfection. Total proteins were separated using SDS-PAGE, and Western blot analysis with specific antibodies was used to identify reovirus proteins and β actin (loading control) (left). Densitometric band quantification of μ1C and δ and percent virus uncoating (right) were calculated using the following formula; [δ/(μ1C + δ)] ×100 ( n = 4 ± standard deviation; MOI of 1, n = 2; MOI of 3, n = 2). Linear regression analysis determined that the slopes are not significantly different. (B to D) L929 cells were exposed to T3D PL , T3D KC , and T3D TD (MOI of 3) at 4°C for 1 h, washed, and incubated at 37°C. At indicated time points, following total RNA extraction and cDNA synthesis, reovirus S4 and M2 RNA expression levels relative to the level of the housekeeping gene GAPDH were quantified using RT-qPCR ( n = 3 ± standard deviation; ***, P

    Article Snippet: Using 10 μg of glycogen (R0551; ThermoFisher Scientific) as a carrier according to manufacturer’s instructions, RNA was purified and converted to cDNA (28025013; ThermoFisher Scientific) using random primers (48190011; ThermoFisher Scientific), and RT-PCR (1725204; Bio-Rad) was performed to quantify reovirus S4, reovirus M2, and mouse GAPDH.

    Techniques: Incubation, SDS Page, Western Blot, Standard Deviation, RNA Extraction, RNA Expression, Quantitative RT-PCR