molecular weight cut off filter  (Millipore)


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    Structured Review

    Millipore molecular weight cut off filter
    Molecular Weight Cut Off Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight cut off filter/product/Millipore
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    molecular weight cut off filter - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Source and Purity of Dengue-Viral Preparations Impact Requirement for Enhancing Antibody to Induce Elevated IL-1β Secretion: A Primary Human Monocyte Model
    Article Snippet: .. Briefly, crude supernatants from DENV-infected Vero cells were concentrated by centrifugation at 1500 x g for 20–25 minutes in 15 ml Millipore Amicon Centrifugal Filter Units with a 100-kDa cutoff, allowing components < 100 kDa to pass through but not virus. ..

    Filtration:

    Article Title: Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein
    Article Snippet: .. Likewise GrB activity was determined in MBP-fur-GrB samples containing equivalent protein amounts but enriched for either unprocessed (retentate) or processed MBP-fur-GrB protein (filtrate) by filtration of culture supernatants through Amicon Ultra centrifugal filters with 100 kDa membranes (Millipore, Schwalbach, Germany). .. A highly purified recombinant GrB derivative was included as a positive control.

    Purification:

    Article Title: Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes
    Article Snippet: .. Therefore, ssDNA_ODN in the eluates was concentrated and purified using Amicon Ultra 0.5 mL 30K centrifugal filters (Millipore GmbH, Schwalbach/Ts., Germany) according to the manufacturer's instructions to prevent an effect on qPCR. .. The used ssDNA_ODN had a length of 90 nucleotides and an approximate molecular weight of 29.7 kDa.

    Real-time Polymerase Chain Reaction:

    Article Title: Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes
    Article Snippet: .. Therefore, ssDNA_ODN in the eluates was concentrated and purified using Amicon Ultra 0.5 mL 30K centrifugal filters (Millipore GmbH, Schwalbach/Ts., Germany) according to the manufacturer's instructions to prevent an effect on qPCR. .. The used ssDNA_ODN had a length of 90 nucleotides and an approximate molecular weight of 29.7 kDa.

    Concentration Assay:

    Article Title: Improved sensitivity of the urine CAA lateral-flow assay for diagnosing active Schistosoma infections by using larger sample volumes
    Article Snippet: .. This step involves the concentration of the TCA-soluble fraction of urine samples using Amicon Ultra Centrifugal Filter Devices (Millipore Corp.), allowing larger sample input, increasing the volume from 10 μL urine to 250 and 2000 μL, or even 7500 μL. .. The results confirm that the larger sample input identified samples with CAA concentrations well below the 30 pg/mL cutoff threshold as maintained for the standard dry-reagent UCP-LF CAA assay without concentration and requiring only 10 μL urine (UCAA10) [ ].

    Activity Assay:

    Article Title: Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein
    Article Snippet: .. Likewise GrB activity was determined in MBP-fur-GrB samples containing equivalent protein amounts but enriched for either unprocessed (retentate) or processed MBP-fur-GrB protein (filtrate) by filtration of culture supernatants through Amicon Ultra centrifugal filters with 100 kDa membranes (Millipore, Schwalbach, Germany). .. A highly purified recombinant GrB derivative was included as a positive control.

    Expressing:

    Article Title: RANTES/CCL5 Induces Collagen Degradation by Activating MMP-1 and MMP-13 Expression in Human Rheumatoid Arthritis Synovial Fibroblasts
    Article Snippet: .. For some experiments, RASFs were pretreated with the inhibitor of p38 (SB203980; 10 µM), ERK (PD98059; 10 µM), JNK (SP600125; 10 µM), NF-KB (PDTC; 200 µM), or PKCδ (Rottlerin; 10 µM) for 2 h followed by RANTES/CCL5 treatment for 24 h. Culture supernatants were concentrated using Amicon® Ultra centrifugal filters (Millipore) and MMP-1 and MMP-13 expression was determined using Western immunoblotting. ..

    Article Title: Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein
    Article Snippet: .. Yeast culture supernatants were collected after induction of protein expression for 3 days, 10×concentrated using Amicon Ultra centrifugal filters with 10 kDa membranes (Millipore), and dialyzed against PBS. ..

    Article Title: RANTES/CCL5 Induces Collagen Degradation by Activating MMP-1 and MMP-13 Expression in Human Rheumatoid Arthritis Synovial Fibroblasts
    Article Snippet: .. Culture supernatant were concentrated using Amicon® Ultra Centrifugal filters (Millipore) and MMP-1 and MMP-13 expression was determined using Western immunoblotting. .. Transient Transfection of siRNA To study the effect of RANTES/CCL5 knockdown on MMP-1 and MMP-13 production, RASFs were transfected with ON-TARGET plus SMART pool RANTES/CCL5 siRNA (GE Dharmacon, Lafayette, CO, USA) using Lipofectamine® RNAiMAX (Life Technologies) for 48 h and then stimulated with IL-1β (5 ng/ml) for 24 h. Conditioned media was used to study the effect of MMP-1 and MMP-13 production using ELISA.

    Western Blot:

    Article Title: RANTES/CCL5 Induces Collagen Degradation by Activating MMP-1 and MMP-13 Expression in Human Rheumatoid Arthritis Synovial Fibroblasts
    Article Snippet: .. For some experiments, RASFs were pretreated with the inhibitor of p38 (SB203980; 10 µM), ERK (PD98059; 10 µM), JNK (SP600125; 10 µM), NF-KB (PDTC; 200 µM), or PKCδ (Rottlerin; 10 µM) for 2 h followed by RANTES/CCL5 treatment for 24 h. Culture supernatants were concentrated using Amicon® Ultra centrifugal filters (Millipore) and MMP-1 and MMP-13 expression was determined using Western immunoblotting. ..

    Article Title: RANTES/CCL5 Induces Collagen Degradation by Activating MMP-1 and MMP-13 Expression in Human Rheumatoid Arthritis Synovial Fibroblasts
    Article Snippet: .. Culture supernatant were concentrated using Amicon® Ultra Centrifugal filters (Millipore) and MMP-1 and MMP-13 expression was determined using Western immunoblotting. .. Transient Transfection of siRNA To study the effect of RANTES/CCL5 knockdown on MMP-1 and MMP-13 production, RASFs were transfected with ON-TARGET plus SMART pool RANTES/CCL5 siRNA (GE Dharmacon, Lafayette, CO, USA) using Lipofectamine® RNAiMAX (Life Technologies) for 48 h and then stimulated with IL-1β (5 ng/ml) for 24 h. Conditioned media was used to study the effect of MMP-1 and MMP-13 production using ELISA.

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    Millipore molecular weight cut off filters
    Mouse urine analysis workflow. Urine samples were collected from four individual mice. Urinary metabolites obtained from 3 kDa <t>molecular</t> <t>weight</t> <t>cut-off</t> ultracentrifugation were labeled separately with 4-plex DiLeu, combined, and purified prior to LC-MS/MS
    Molecular Weight Cut Off Filters, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight cut off filters/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    molecular weight cut off filters - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Millipore 100 kd molecular weight cut off
    Electron microscopy of the rotary shadowed procollagen molecules. All procollagens had an extended structure. Globular C-propeptide is apparent in all procollagen variants. Pro II indicates procollagen II; mD4, mD4 procollagen; and mD4M, mD4M procollagen. The mean length of the mD4 procollagen was 290.3 nm (±4.04 SEM, n = 33), the mean length of the mD4M was 336 nm (±3.37 SEM, n = 15), and the mean length of normal procollagen II was 302 nm (±4.31 SEM, n = 16). Bar, <t>100</t> nm.
    100 Kd Molecular Weight Cut Off, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 kd molecular weight cut off/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    100 kd molecular weight cut off - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Millipore cut off molecular weight 10
    Electron microscopy of the rotary shadowed procollagen molecules. All procollagens had an extended structure. Globular C-propeptide is apparent in all procollagen variants. Pro II indicates procollagen II; mD4, mD4 procollagen; and mD4M, mD4M procollagen. The mean length of the mD4 procollagen was 290.3 nm (±4.04 SEM, n = 33), the mean length of the mD4M was 336 nm (±3.37 SEM, n = 15), and the mean length of normal procollagen II was 302 nm (±4.31 SEM, n = 16). Bar, <t>100</t> nm.
    Cut Off Molecular Weight 10, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cut off molecular weight 10/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cut off molecular weight 10 - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Mouse urine analysis workflow. Urine samples were collected from four individual mice. Urinary metabolites obtained from 3 kDa molecular weight cut-off ultracentrifugation were labeled separately with 4-plex DiLeu, combined, and purified prior to LC-MS/MS

    Journal: The Analyst

    Article Title: Relative quantification of amine-containing metabolites using isobaric N,N-dimethyl-leucine (DiLeu) reagents via LC-ESI-MS/MS and CE-ESI-MS/MS

    doi: 10.1039/c4an01582g

    Figure Lengend Snippet: Mouse urine analysis workflow. Urine samples were collected from four individual mice. Urinary metabolites obtained from 3 kDa molecular weight cut-off ultracentrifugation were labeled separately with 4-plex DiLeu, combined, and purified prior to LC-MS/MS

    Article Snippet: Metabolite fractions of urine were obtained by using 3 kDa molecular weight cut-off filters (Millipore Amicon Ultra) to remove urinary proteins.

    Techniques: Mouse Assay, Molecular Weight, Labeling, Purification, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Proteinase K digestion profiles of α-syn aggregates after several rounds of α-syn-PMCA. This is the same experiment as , showing the results obtained with samples from different patients with PD ( n = 3) and patients with MSA ( n = 3). The first round corresponds to direct amplification from the CSF of the patients. For the second round of amplification, aggregates produced in the first round were diluted 100-fold into fresh α-syn monomer substrate and a new round of α-syn-PMCA was performed. The assay was then repeated for the third and fourth rounds using amplified α-syn aggregates (1%) from the previous round. Amplified aggregates were treated with proteinase K (1 mg ml −1 ) for 1 h and proteins were separated on a 12% Bis-Tris gel and immunoblotted with the BD anti-α-syn clone 42 antibody. Molecular weight markers (kDa) are indicated on the left of the blot. Fig. 2e

    Journal: Nature

    Article Title: Discriminating α-synuclein strains in Parkinson’s disease and multiple system atrophy

    doi: 10.1038/s41586-020-1984-7

    Figure Lengend Snippet: Proteinase K digestion profiles of α-syn aggregates after several rounds of α-syn-PMCA. This is the same experiment as , showing the results obtained with samples from different patients with PD ( n = 3) and patients with MSA ( n = 3). The first round corresponds to direct amplification from the CSF of the patients. For the second round of amplification, aggregates produced in the first round were diluted 100-fold into fresh α-syn monomer substrate and a new round of α-syn-PMCA was performed. The assay was then repeated for the third and fourth rounds using amplified α-syn aggregates (1%) from the previous round. Amplified aggregates were treated with proteinase K (1 mg ml −1 ) for 1 h and proteins were separated on a 12% Bis-Tris gel and immunoblotted with the BD anti-α-syn clone 42 antibody. Molecular weight markers (kDa) are indicated on the left of the blot. Fig. 2e

    Article Snippet: To remove any preformed seeds or aggregates, the protein solution was filtered through a 100-kDa cut-off filter (Amicon Ultra, Millipore), separated into small aliquots and stored at −80 °C until use.

    Techniques: Amplification, Produced, Molecular Weight

    Electron microscopy of the rotary shadowed procollagen molecules. All procollagens had an extended structure. Globular C-propeptide is apparent in all procollagen variants. Pro II indicates procollagen II; mD4, mD4 procollagen; and mD4M, mD4M procollagen. The mean length of the mD4 procollagen was 290.3 nm (±4.04 SEM, n = 33), the mean length of the mD4M was 336 nm (±3.37 SEM, n = 15), and the mean length of normal procollagen II was 302 nm (±4.31 SEM, n = 16). Bar, 100 nm.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Prospects and limitations of the rational engineering of fibrillar collagens

    doi:

    Figure Lengend Snippet: Electron microscopy of the rotary shadowed procollagen molecules. All procollagens had an extended structure. Globular C-propeptide is apparent in all procollagen variants. Pro II indicates procollagen II; mD4, mD4 procollagen; and mD4M, mD4M procollagen. The mean length of the mD4 procollagen was 290.3 nm (±4.04 SEM, n = 33), the mean length of the mD4M was 336 nm (±3.37 SEM, n = 15), and the mean length of normal procollagen II was 302 nm (±4.31 SEM, n = 16). Bar, 100 nm.

    Article Snippet: High-molecular-weight proteins in the medium were concentrated ∼10-fold at 4°C by the use of cartridges with a 100-kD molecular-weight cut-off (Prep/Scale-TFF filter; Millipore).

    Techniques: Electron Microscopy